(Circular) (Six-base) MAPSORT of: pFN21AE3154.seq Check: 9196 from: 1 to: 5821 pFN21AE3154 5821 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2249 3631 278 Size: 53 83 186 936 713 1382 2468 Fragments arranged by size: 2468 1382 936 713 186 83 53 AccI GT'mk_AC Cuts at: 1937 3164 1937 Size: 1227 4594 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3884 5268 5536 1632 Size: 2252 1384 268 1917 Fragments arranged by size: 2252 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5821 AlwNI CAG_nnn'CTG Cuts at: 3564 5136 3564 Size: 1572 4249 ApaI G_GGCC'C Cuts at: 2440 2674 2440 Size: 234 5587 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 3130 3356 3508 5617 1493 Size: 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 226 152 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 2340 2428 2537 2667 2792 2989 3146 Size: 73 397 88 109 130 125 197 157 Cuts at: 3146 1870 Size: 4545 Fragments arranged by size: 4545 397 197 157 130 125 109 88 73 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2193 2563 2603 2847 Size: 325 217 627 369 37 370 40 244 Cuts at: 2847 3142 4597 618 Size: 295 1455 1842 Fragments arranged by size: 1842 1455 627 370 369 325 295 244 217 40 37 BanII G_rGCy'C Cuts at: 1391 2151 2440 2617 2674 3140 1391 Size: 760 289 177 57 466 4072 Fragments arranged by size: 4072 760 466 289 177 57 BbeI G_GCGC'C Cuts at: 2197 2607 2197 Size: 410 5411 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2386 928 Size: 971 487 4363 Fragments arranged by size: 4363 971 487 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4012 4046 1903 Size: 34 50 34 1991 34 3678 Fragments arranged by size: 3678 1991 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2612 2796 4432 Size: 107 122 71 631 605 940 184 1636 Cuts at: 4432 136 Size: 1525 Fragments arranged by size: 1636 1525 940 631 605 184 122 107 71 BglII A'GATC_T Cuts at: 5816 5816 Size: 5821 BlpI GC'TnA_GC Cuts at: 2421 2498 3238 2421 Size: 77 740 5004 Fragments arranged by size: 5004 740 77 Bme1580I G_kGCm'C Cuts at: 1790 2100 2161 2440 2568 2674 3021 3882 5038 Size: 310 61 279 128 106 347 861 1156 Cuts at: 5038 1790 Size: 2573 Fragments arranged by size: 2573 1156 861 347 310 279 128 106 61 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3552 4510 442 Size: 416 53 83 399 24 2135 958 1753 Fragments arranged by size: 2135 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2374 2374 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2465 4481 1239 Size: 869 357 2016 2579 Fragments arranged by size: 2579 2016 869 357 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3946 4811 5109 3946 Size: 865 298 4658 Fragments arranged by size: 4658 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2216 4484 882 Size: 617 717 2268 2219 Fragments arranged by size: 2268 2219 717 617 BsaAI yAC'GTr Cuts at: 493 1204 2278 2824 3612 493 Size: 711 1074 546 788 2702 Fragments arranged by size: 2702 1074 788 711 546 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2194 2206 2246 2604 Size: 53 83 186 936 661 12 40 358 Cuts at: 2604 2766 3628 4010 4645 275 Size: 162 862 382 635 1451 Fragments arranged by size: 1451 936 862 661 635 382 358 186 162 83 53 40 12 BsaWI w'CCGG_w Cuts at: 4254 4652 4926 5073 5484 4254 Size: 398 274 147 411 4591 Fragments arranged by size: 4591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2213 2243 1178 Size: 30 165 30 810 30 4756 Fragments arranged by size: 4756 810 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2036 2039 2297 1186 Size: 847 3 3 258 4710 Fragments arranged by size: 4710 847 258 3 3 BseYI C'CCAG_C Cuts at: 2107 2287 2490 2599 2853 5024 5520 2107 Size: 180 203 109 254 2171 496 2408 Fragments arranged by size: 2408 2171 496 254 203 180 109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5248 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2174 2364 2756 2985 3271 4032 Size: 275 186 173 190 392 229 286 761 Cuts at: 4032 5060 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 392 286 275 229 190 186 173 BsiHKAI G_wGCw'C Cuts at: 3048 3882 3967 5038 3048 Size: 834 85 1071 3831 Fragments arranged by size: 3831 1071 834 85 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2151 2161 2440 2568 2617 2674 Size: 399 310 51 10 279 128 49 57 Cuts at: 2674 3021 3048 3140 3882 3967 5038 1391 Size: 347 27 92 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 347 310 279 128 92 85 57 51 49 27 10 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3012 3176 844 Size: 2168 164 3489 Fragments arranged by size: 3489 2168 164 BsrBI CCG'CTC Cuts at: 1394 1823 3708 1394 Size: 429 1885 3507 Fragments arranged by size: 3507 1885 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2371 4316 4490 65 Size: 1183 1123 1945 174 1396 Fragments arranged by size: 1945 1396 1183 1123 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2173 2542 2941 4465 4652 1700 Size: 110 363 369 399 1524 187 2869 Fragments arranged by size: 2869 1524 399 369 363 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 2587 2685 2687 2808 2904 2965 2967 3023 Size: 662 98 2 121 96 61 2 56 Cuts at: 3023 1925 Size: 4723 Fragments arranged by size: 4723 662 121 98 96 61 56 2 2 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5821 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 2257 2716 1184 Size: 1073 459 4289 Fragments arranged by size: 4289 1073 459 BstYI r'GATC_y Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816 Size: 1179 367 385 17 1452 86 12 346 Cuts at: 5816 1972 Size: 1977 Fragments arranged by size: 1977 1452 1179 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2326 2364 2756 513 Size: 552 1261 38 392 3578 Fragments arranged by size: 3578 1261 552 392 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2157 2845 487 Size: 683 56 931 688 3463 Fragments arranged by size: 3463 931 688 683 56 BtsI GCAGTG_nn' Cuts at: 3337 4131 4151 3337 Size: 794 20 5007 Fragments arranged by size: 5007 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DraIII CAC_nnn'GTG Cuts at: 2959 2959 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4828 809 Size: 507 3512 1802 Fragments arranged by size: 3512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2171 2361 Size: 54 1425 50 275 18 202 139 190 Cuts at: 2361 2630 2694 2753 2880 2982 3268 4157 5786 Size: 269 64 59 127 102 286 889 1629 Cuts at: 5786 8 Size: 43 Fragments arranged by size: 1629 1425 889 286 275 269 202 190 139 127 102 64 59 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2171 2361 2753 2982 3268 1537 Size: 275 359 190 392 229 286 4090 Fragments arranged by size: 4090 392 359 286 275 229 190 EarI CTCTTCn'nnn_ Cuts at: 1353 3053 3751 1353 Size: 1700 698 3423 Fragments arranged by size: 3423 1700 698 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2257 2543 2633 4408 4782 4928 Size: 1064 138 668 286 90 1775 374 146 Cuts at: 4928 387 Size: 1280 Fragments arranged by size: 1775 1280 1064 668 374 286 146 138 90 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2465 3884 4481 5268 5536 1239 Size: 393 476 357 1419 597 787 268 1524 Fragments arranged by size: 1524 1419 787 597 476 393 357 268 EcoNI CCTnn'n_nnAGG Cuts at: 2123 2696 2123 Size: 573 5248 EcoO109I rG'GnC_Cy Cuts at: 2024 2212 2671 2024 Size: 188 459 5174 Fragments arranged by size: 5174 459 188 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 2197 2428 2607 2654 2660 2802 2948 4968 Size: 519 231 179 47 6 142 146 2020 Cuts at: 4968 1678 Size: 2531 Fragments arranged by size: 2531 2020 519 231 179 146 142 47 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 4462 4494 4536 Size: 32 135 32 641 32 2217 32 42 Cuts at: 4536 4568 1373 Size: 32 2626 Fragments arranged by size: 2626 2217 641 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3165 3411 669 Size: 988 281 1227 246 3079 Fragments arranged by size: 3079 1227 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KasI G'GCGC_C Cuts at: 2193 2603 2193 Size: 410 5411 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4935 5119 1305 Size: 3630 184 2007 Fragments arranged by size: 3630 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2882 10 Size: 54 1425 343 1050 2949 Fragments arranged by size: 2949 1425 1050 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3041 3098 3779 4138 4297 518 Size: 648 1875 57 681 359 159 2042 Fragments arranged by size: 2042 1875 681 648 359 159 57 MspA1I CmG'CkG Cuts at: 1606 2328 2366 2420 2758 2853 3237 3914 5062 Size: 722 38 54 338 95 384 677 1148 Cuts at: 5062 5307 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 722 677 384 338 245 95 54 38 NaeI GCC'GGC Cuts at: 2943 2943 Size: 5821 NarI GG'CG_CC Cuts at: 2194 2604 2194 Size: 410 5411 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5269 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NgoMIV G'CCGG_C Cuts at: 2941 2941 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 2982 3268 2982 Size: 286 5535 NspI r_CATG'y Cuts at: 1132 1229 3179 4724 1132 Size: 97 1950 1545 2229 Fragments arranged by size: 2229 1950 1545 97 PasI CC'CwG_GG Cuts at: 1348 2152 2252 2572 1348 Size: 804 100 320 4597 Fragments arranged by size: 4597 804 320 100 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 2746 1108 Size: 1638 4183 PfoI T'CCnGG_A Cuts at: 2319 2319 Size: 5821 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PmlI CAC'GTG Cuts at: 2278 2278 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2024 2212 2024 Size: 188 5633 PshAI GACnn'nnGTC Cuts at: 1613 2390 1613 Size: 777 5044 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PspOMI G'GGCC_C Cuts at: 2436 2670 2436 Size: 234 5587 PstI C_TGCA'G Cuts at: 830 3173 830 Size: 2343 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 RsrII CG'GwC_CG Cuts at: 2323 2323 Size: 5821 SacII CC_GC'GG Cuts at: 2329 2367 2759 2329 Size: 38 392 5391 Fragments arranged by size: 5391 392 38 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SanDI GG'GwC_CC Cuts at: 2212 2212 Size: 5821 SapI GCTCTTCn'nnn_ Cuts at: 3053 3053 Size: 5821 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SexAI A'CCwGG_T Cuts at: 2225 2225 Size: 5821 SfcI C'TryA_G Cuts at: 826 1045 3169 4304 4985 5176 826 Size: 219 2124 1135 681 191 1471 Fragments arranged by size: 2124 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2195 2605 2195 Size: 410 5411 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 2941 1700 Size: 1241 4580 SmaI CCC'GGG Cuts at: 1872 2342 2539 2669 2794 2991 3148 1872 Size: 470 197 130 125 197 157 4545 Fragments arranged by size: 4545 470 197 197 157 130 125 SmlI C'TyrA_G Cuts at: 820 1017 1943 3925 4826 5088 820 Size: 197 926 1982 901 262 1553 Fragments arranged by size: 1982 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 2702 1517 Size: 1185 4636 StyI C'CwwG_G Cuts at: 513 1065 3260 513 Size: 552 2195 3074 Fragments arranged by size: 3074 2195 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3848 3865 4018 4203 4648 325 Size: 1255 2268 17 153 185 445 1498 Fragments arranged by size: 2268 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4067 5779 96 Size: 274 80 33 51 1443 2090 1712 138 Fragments arranged by size: 2090 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2292 2324 5781 5813 2292 Size: 32 3457 32 2300 Fragments arranged by size: 3457 2300 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2227 1316 Size: 911 4910 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 2340 2537 2667 2792 2989 3146 1870 Size: 470 197 130 125 197 157 4545 Fragments arranged by size: 4545 470 197 197 157 130 125 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2247 3629 276 Size: 53 83 186 936 713 1382 2468 Fragments arranged by size: 2468 1382 936 713 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI PvuII SacI SfiI SrfI SwaI XcmI