(Linear) (Six-base) MAPSORT of: KIEE3154.seq Check: 322 from: 1 to: 1116 KIEE3154.orf With 182 enzymes: SgfI * July 3, 2012 17:23 .. AatII G_ACGT'C Cuts at: 0 244 1116 Size: 244 872 ApaI G_GGCC'C Cuts at: 0 435 669 1116 Size: 435 234 447 Fragments arranged by size: 447 435 234 AvaI C'yCGr_G Cuts at: 0 335 423 532 662 787 984 1116 Size: 335 88 109 130 125 197 132 Fragments arranged by size: 335 197 132 130 125 109 88 BanI G'GyrC_C Cuts at: 0 151 188 558 598 842 1116 Size: 151 37 370 40 244 274 Fragments arranged by size: 370 274 244 151 40 37 BanII G_rGCy'C Cuts at: 0 146 435 612 669 1116 Size: 146 289 177 57 447 Fragments arranged by size: 447 289 177 146 57 BbeI G_GCGC'C Cuts at: 0 192 602 1116 Size: 192 410 514 Fragments arranged by size: 514 410 192 BbsI GAAGACnn'nnnn_ Cuts at: 0 381 1116 Size: 381 735 BglI GCCn_nnn'nGGC Cuts at: 0 607 791 1116 Size: 607 184 325 Fragments arranged by size: 607 325 184 BlpI GC'TnA_GC Cuts at: 0 416 493 1116 Size: 416 77 623 Fragments arranged by size: 623 416 77 Bme1580I G_kGCm'C Cuts at: 0 95 156 435 563 669 1016 1116 Size: 95 61 279 128 106 347 100 Fragments arranged by size: 347 279 128 106 100 95 61 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 369 1116 Size: 369 747 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 460 1116 Size: 103 357 656 Fragments arranged by size: 656 357 103 BsaI GGTCTCn'nnnn_ Cuts at: 0 211 1116 Size: 211 905 BsaAI yAC'GTr Cuts at: 0 273 819 1116 Size: 273 546 297 Fragments arranged by size: 546 297 273 BsaHI Gr'CG_yC Cuts at: 0 189 201 241 599 761 1116 Size: 189 12 40 358 162 355 Fragments arranged by size: 358 355 189 162 40 12 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 208 238 1116 Size: 208 30 878 Fragments arranged by size: 878 208 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 28 31 34 292 1116 Size: 28 3 3 258 824 Fragments arranged by size: 824 258 28 3 3 BseYI C'CCAG_C Cuts at: 0 102 282 485 594 848 1116 Size: 102 180 203 109 254 268 Fragments arranged by size: 268 254 203 180 109 102 BsiEI CG_ry'CG Cuts at: 0 169 359 751 980 1116 Size: 169 190 392 229 136 Fragments arranged by size: 392 229 190 169 136 BsiHKAI G_wGCw'C Cuts at: 0 1043 1116 Size: 1043 73 Bsp1286I G_dGCh'C Cuts at: 0 95 146 156 435 563 612 669 1016 Size: 95 51 10 279 128 49 57 347 Cuts at: 1016 1043 1116 Size: 27 73 Fragments arranged by size: 347 279 128 95 73 57 51 49 27 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1007 1116 Size: 1007 109 BsrDI GCAATG_nn' Cuts at: 0 366 1116 Size: 366 750 BsrFI r'CCGG_y Cuts at: 0 168 537 936 1116 Size: 168 369 399 180 Fragments arranged by size: 399 369 180 168 BssHII G'CGCG_C Cuts at: 0 582 680 682 803 899 960 962 1018 Size: 582 98 2 121 96 61 2 56 Cuts at: 1018 1116 Size: 98 Fragments arranged by size: 582 121 98 98 96 61 56 2 2 BstEII G'GTnAC_C Cuts at: 0 252 711 1116 Size: 252 459 405 Fragments arranged by size: 459 405 252 BtgI C'CryG_G Cuts at: 0 321 359 751 1116 Size: 321 38 392 365 Fragments arranged by size: 392 365 321 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 152 840 1116 Size: 152 688 276 Fragments arranged by size: 688 276 152 DraIII CAC_nnn'GTG Cuts at: 0 954 1116 Size: 954 162 EaeI y'GGCC_r Cuts at: 0 27 166 356 625 689 748 875 977 Size: 27 139 190 269 64 59 127 102 Cuts at: 977 1116 Size: 139 Fragments arranged by size: 269 190 139 139 127 102 64 59 27 EagI C'GGCC_G Cuts at: 0 166 356 748 977 1116 Size: 166 190 392 229 139 Fragments arranged by size: 392 229 190 166 139 EarI CTCTTCn'nnn_ Cuts at: 0 1048 1116 Size: 1048 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 252 538 628 1116 Size: 252 286 90 488 Fragments arranged by size: 488 286 252 90 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 460 1116 Size: 103 357 656 Fragments arranged by size: 656 357 103 EcoNI CCTnn'n_nnAGG Cuts at: 0 118 691 1116 Size: 118 573 425 Fragments arranged by size: 573 425 118 EcoO109I rG'GnC_Cy Cuts at: 0 19 207 666 1116 Size: 19 188 459 450 Fragments arranged by size: 459 450 188 19 HaeII r_GCGC'y Cuts at: 0 192 423 602 649 655 797 943 1116 Size: 192 231 179 47 6 142 146 173 Fragments arranged by size: 231 192 179 173 146 142 47 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 208 240 1116 Size: 208 32 876 Fragments arranged by size: 876 208 32 KasI G'GCGC_C Cuts at: 0 188 598 1116 Size: 188 410 518 Fragments arranged by size: 518 410 188 MscI TGG'CCA Cuts at: 0 877 1116 Size: 877 239 MslI CAynn'nnrTG Cuts at: 0 1036 1093 1116 Size: 1036 57 23 Fragments arranged by size: 1036 57 23 MspA1I CmG'CkG Cuts at: 0 323 361 415 753 848 1116 Size: 323 38 54 338 95 268 Fragments arranged by size: 338 323 268 95 54 38 NaeI GCC'GGC Cuts at: 0 938 1116 Size: 938 178 NarI GG'CG_CC Cuts at: 0 189 599 1116 Size: 189 410 517 Fragments arranged by size: 517 410 189 NgoMIV G'CCGG_C Cuts at: 0 936 1116 Size: 936 180 NotI GC'GGCC_GC Cuts at: 0 977 1116 Size: 977 139 PasI CC'CwG_GG Cuts at: 0 147 247 567 1116 Size: 147 100 320 549 Fragments arranged by size: 549 320 147 100 PflMI CCAn_nnn'nTGG Cuts at: 0 741 1116 Size: 741 375 PfoI T'CCnGG_A Cuts at: 0 314 1116 Size: 314 802 PmlI CAC'GTG Cuts at: 0 273 1116 Size: 273 843 PpuMI rG'GwC_Cy Cuts at: 0 19 207 1116 Size: 19 188 909 Fragments arranged by size: 909 188 19 PshAI GACnn'nnGTC Cuts at: 0 385 1116 Size: 385 731 PspOMI G'GGCC_C Cuts at: 0 431 665 1116 Size: 431 234 451 Fragments arranged by size: 451 431 234 RsrII CG'GwC_CG Cuts at: 0 318 1116 Size: 318 798 SacII CC_GC'GG Cuts at: 0 324 362 754 1116 Size: 324 38 392 362 Fragments arranged by size: 392 362 324 38 SanDI GG'GwC_CC Cuts at: 0 207 1116 Size: 207 909 SapI GCTCTTCn'nnn_ Cuts at: 0 1048 1116 Size: 1048 68 SexAI A'CCwGG_T Cuts at: 0 220 1116 Size: 220 896 SfoI GGC'GCC Cuts at: 0 190 600 1116 Size: 190 410 516 Fragments arranged by size: 516 410 190 SgrAI Cr'CCGG_yG Cuts at: 0 936 1116 Size: 936 180 SmaI CCC'GGG Cuts at: 0 337 534 664 789 986 1116 Size: 337 197 130 125 197 130 Fragments arranged by size: 337 197 197 130 130 125 StuI AGG'CCT Cuts at: 0 697 1116 Size: 697 419 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 287 319 1116 Size: 287 32 797 Fragments arranged by size: 797 287 32 Tth111I GACn'n_nGTC Cuts at: 0 222 1116 Size: 222 894 XmaI C'CCGG_G Cuts at: 0 335 532 662 787 984 1116 Size: 335 197 130 125 197 132 Fragments arranged by size: 335 197 197 132 130 125 ZraI GAC'GTC Cuts at: 0 242 1116 Size: 242 874 Enzymes that do cut: AatII ApaI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BplI BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssHII BstEII BtgI BtgZI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV NotI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI RsrII SacII SanDI SapI SexAI SfoI SgrAI SmaI StuI TstI Tth111I XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI Bpu10I BpuEI BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NruI NsiI NspI PacI PciI PmeI PpiI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII TatI XbaI XcmI XhoI XmnI