################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaHI BsaI BseAI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Cac8I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III EcoNI EcoO109I EcoRI FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI KspI MaeI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 848 GTAGAC GT^[AC][GT]AC AgeI 722 ACCGGT A^CCGGT AocI 752 CCTTAGG CC^T[ACGT]AGG AvaII 207 GGACC G^G[AT]CC BbsI 790 GAAGAC GAAGAC(2/6) BcgI 951 GCATTTCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 21 CTGGAG CTGGAG(16/14) BpuAI 790 GAAGAC GAAGAC(2/6) BsaBI 71 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 305 CCTTGG C^C[ACGT][ACGT]GG BsaWI 722 ACCGGT [AT]^CCGG[AT] BseRI 554 GAGGAG GAGGAG(10/8) BsiEI 1010 CGGCCG CG[GA][CT]^CG BsmFI 740 GGGAC GGGAC(10/14) BsrFI 722 ACCGGT [GA]^CCGG[CT] BstEII 762 GGTGACC G^GT[ACGT]ACC BstPI 762 GGTGACC G^GT[ACGT]ACC Bsu36I 752 CCTTAGG CC^T[ACGT]AGG Cfr10I 722 ACCGGT [GA]^CCGG[CT] Cfr13I 207 GGACC G^G[ACGT]CC EaeI 1010 CGGCCG [CT]^GGCC[GA] EagI 1010 CGGCCG C^GGCCG EclXI 1010 CGGCCG C^GGCCG Eco52I 1010 CGGCCG C^GGCCG Eco81I 752 CCTTAGG CC^T[ACGT]AGG EcoO65I 762 GGTGACC G^GT[ACGT]ACC EcoRV 352 GATATC GAT^ATC EcoT14I 305 CCTTGG C^C[AT][AT]GG EcoT22I 185 ATGCAT ATGCA^T HapII 723 CCGG C^CGG HincII 408 GTTAAC GT[CT]^[GA]AC HindII 408 GTTAAC GT[CT]^[GA]AC HindIII 811 AAGCTT A^AGCTT HpaI 408 GTTAAC GTT^AAC HpaII 723 CCGG C^CGG MaeII 772 ACGT A^CGT MamI 71 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 723 CCGG C^CGG MwoI 627 GCAACAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NsiI 185 ATGCAT ATGCA^T NspI 701 ACATGC [GA]CATG^[CT] PinAI 722 ACCGGT A^CCGGT PleI 988 GACTC GAGTC(4/5) PstI 966 CTGCAG CTGCA^G SapI 172 GAAGAGC GCTCTTC(1/4) Sau96I 207 GGACC G^G[ACGT]CC SfaNI 187 GCATC GCATC(5/9) SmlI 336 CTCAAG C^T[CT][GA]AG StyI 305 CCTTGG C^C[AT][AT]GG TfiI 76 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 1045 CCGC CCGC(-3/-1) 1009 GCGG Asp700I 438 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 610 GAAACTATTC BglII 25 AGATCT A^GATCT 510 AGATCT BsgI 697 CTGCAC GTGCAG(16/14) 1002 CTGCAC BsmAI 941 GAGAC GTCTC(1/5) 1041 GTCTC BsmI 542 GCATTC GAATGC(1/-1) 971 GCATTC BsrI 20 ACTGG ACTGG(1/-1) 405 CCAGT HinfI 76 GAATC G^A[ACGT]TC 988 GACTC MslI 178 CATGAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1032 CACGCCTGTG MspA1I 799 CAGCTG C[AC]G^C[GT]G 1023 CAGCTG PvuII 799 CAGCTG CAG^CTG 1023 CAGCTG SfcI 637 CTACAG C^T[GA][CT]AG 966 CTGCAG SspI 159 AATATT AAT^ATT 589 AATATT TaqI 190 TCGA T^CGA 1058 TCGA TthHB8I 190 TCGA T^CGA 1058 TCGA XmnI 438 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 610 GAAACTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AfaI AluI AlwI BbvI BsiYI BslI BssKI BstF5I BstNI BstYI DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MvaI NdeII NlaIII RsaI Sau3AI ScrFI Tru9I TseI Tsp45I Tsp509I TspRI XhoII