(Circular) (Six-base) MAPSORT of: pFN21AE3150.seq Check: 6677 from: 1 to: 5779 pFN21AE3150 5779 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3589 278 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2854 3122 1937 Size: 917 268 4594 Fragments arranged by size: 4594 917 268 Acc65I G'GTAC_C Cuts at: 3100 3100 Size: 5779 AclI AA'CG_TT Cuts at: 1563 3906 4279 1563 Size: 2343 373 3063 Fragments arranged by size: 3063 2343 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2636 2897 3842 5226 5494 1632 Size: 1004 261 945 1384 268 1917 Fragments arranged by size: 1917 1384 1004 945 268 261 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5582 AflIII A'CryG_T Cuts at: 1203 4678 5655 1203 Size: 3475 977 1327 Fragments arranged by size: 3475 1327 977 AgeI A'CCGG_T Cuts at: 2727 4610 2727 Size: 1883 3896 AhdI GACnn_n'nnGTC Cuts at: 4508 4508 Size: 5779 AleI CACnn'nnGTG Cuts at: 1166 3041 1166 Size: 1875 3904 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2205 2415 1184 Size: 1021 210 4548 Fragments arranged by size: 4548 1021 210 AlwNI CAG_nnn'CTG Cuts at: 3522 5094 3522 Size: 1572 4207 ApaLI G'TGCA_C Cuts at: 3836 4992 3836 Size: 1156 4623 ApoI r'AATT_y Cuts at: 1493 3088 3314 3466 5575 1493 Size: 1595 226 152 2109 1697 Fragments arranged by size: 2109 1697 1595 226 152 AseI AT'TA_AT Cuts at: 160 1032 4333 160 Size: 872 3301 1606 Fragments arranged by size: 3301 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 AvaI C'yCGr_G Cuts at: 1870 1943 3104 1870 Size: 73 1161 4545 Fragments arranged by size: 4545 1161 73 BamHI G'GATC_C Cuts at: 3109 3476 3109 Size: 367 5412 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3100 4555 618 Size: 325 217 627 1313 1455 1842 Fragments arranged by size: 1842 1455 1313 627 325 217 BanII G_rGCy'C Cuts at: 1391 3098 1391 Size: 1707 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2802 928 Size: 971 903 3905 Fragments arranged by size: 3905 971 903 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2945 2979 3970 4004 1903 Size: 34 50 34 924 34 991 34 3678 Fragments arranged by size: 3678 991 924 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3671 4881 3671 Size: 1210 4569 BclI T'GATC_A Cuts at: 1548 1548 Size: 5779 BfrBI ATG'CAT Cuts at: 2192 2192 Size: 5779 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4390 136 Size: 107 122 71 631 605 2718 1525 Fragments arranged by size: 2718 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2030 2515 5774 2030 Size: 485 3259 2035 Fragments arranged by size: 3259 2035 485 BlpI GC'TnA_GC Cuts at: 3196 3196 Size: 5779 Bme1580I G_kGCm'C Cuts at: 1790 3840 4996 1790 Size: 2050 1156 2573 Fragments arranged by size: 2573 2050 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5779 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2404 3510 4468 Size: 416 53 83 399 24 987 1106 958 Cuts at: 4468 442 Size: 1753 Fragments arranged by size: 1753 1106 987 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2047 4439 1239 Size: 808 2392 2579 Fragments arranged by size: 2579 2392 808 Bpu10I CC'TnA_GC Cuts at: 2531 2531 Size: 5779 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2326 3904 4769 5067 2326 Size: 1578 865 298 3038 Fragments arranged by size: 3038 1578 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4442 882 Size: 617 2943 2219 Fragments arranged by size: 2943 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3570 493 Size: 711 2366 2702 Fragments arranged by size: 2702 2366 711 BsaBI GATnn'nnATC Cuts at: 2080 5773 2080 Size: 3693 2086 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3586 3968 4603 275 Size: 53 83 186 936 2053 382 635 1451 Fragments arranged by size: 2053 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2727 4212 4610 4884 5031 5442 2727 Size: 1485 398 274 147 411 3064 Fragments arranged by size: 3064 1485 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5554 Fragments arranged by size: 5554 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2574 1186 Size: 1388 4391 BseYI C'CCAG_C Cuts at: 2926 4982 5478 2926 Size: 2056 496 3227 Fragments arranged by size: 3227 2056 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2687 2992 1163 Size: 573 951 305 3950 Fragments arranged by size: 3950 951 573 305 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3018 3229 3990 5018 1540 Size: 275 186 1017 211 761 1028 2301 Fragments arranged by size: 2301 1028 1017 761 275 211 186 BsiHKAI G_wGCw'C Cuts at: 3840 3925 4996 3840 Size: 85 1071 4623 Fragments arranged by size: 4623 1071 85 BsmI GAATG_Cn' Cuts at: 2547 2976 3290 3383 2547 Size: 429 314 93 4943 Fragments arranged by size: 4943 429 314 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3098 3840 3925 4996 1391 Size: 399 1308 742 85 1071 2174 Fragments arranged by size: 2174 1308 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5442 5442 Size: 5779 BspHI T'CATG_A Cuts at: 3668 5398 3668 Size: 1730 4049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3134 844 Size: 2290 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3666 1394 Size: 429 1843 3507 Fragments arranged by size: 3507 1843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4274 4448 65 Size: 1183 3026 174 1396 Fragments arranged by size: 3026 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2727 4423 4610 1700 Size: 110 917 1696 187 2869 Fragments arranged by size: 2869 1696 917 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5779 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5779 BssSI C'ACGA_G Cuts at: 3839 4851 3839 Size: 1012 4767 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5779 BstBI TT'CG_AA Cuts at: 4583 4583 Size: 5779 BstEII G'GTnAC_C Cuts at: 1184 2767 1184 Size: 1583 4196 BstYI r'GATC_y Cuts at: 1972 2030 2262 2515 2647 3109 3476 3861 3878 Size: 58 232 253 132 462 367 385 17 Cuts at: 3878 5330 5416 5428 5774 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 462 385 367 346 253 232 132 86 58 17 12 Bsu36I CC'TnA_GG Cuts at: 2758 2758 Size: 5779 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5227 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5040 Fragments arranged by size: 5040 683 56 BtsI GCAGTG_nn' Cuts at: 2951 2968 3295 4089 4109 2951 Size: 17 327 794 20 4621 Fragments arranged by size: 4621 794 327 20 17 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5779 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5744 DraI TTT'AAA Cuts at: 3083 3083 Size: 5779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4786 809 Size: 507 3470 1802 Fragments arranged by size: 3470 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3015 3226 4115 Size: 54 1425 50 275 18 1185 211 889 Cuts at: 4115 5744 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1185 889 275 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3015 3226 1537 Size: 275 1203 211 4090 Fragments arranged by size: 4090 1203 275 211 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 2172 3074 3709 1353 Size: 713 106 902 635 3423 Fragments arranged by size: 3423 902 713 635 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4366 4740 4886 387 Size: 1064 138 2777 374 146 1280 Fragments arranged by size: 2777 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2636 2897 3842 4439 5226 5494 Size: 393 415 589 261 945 597 787 268 Cuts at: 5494 1239 Size: 1524 Fragments arranged by size: 1524 945 787 597 589 415 393 268 261 EcoRI G'AATT_C Cuts at: 3088 3466 3088 Size: 378 5401 EcoRV GAT'ATC Cuts at: 2359 2359 Size: 5779 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2464 2464 Size: 5779 FspI TGC'GCA Cuts at: 4285 4285 Size: 5779 HaeII r_GCGC'y Cuts at: 1678 4926 1678 Size: 3248 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2519 2551 2733 Size: 32 135 32 443 32 472 32 182 Cuts at: 2733 2765 2847 2879 4420 4452 4494 4526 1373 Size: 32 82 32 1541 32 42 32 2626 Fragments arranged by size: 2626 1541 472 443 182 135 82 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2415 3123 3369 669 Size: 988 281 477 708 246 3079 Fragments arranged by size: 3079 988 708 477 281 246 HindIII A'AGCT_T Cuts at: 748 2816 748 Size: 2068 3711 HpaI GTT'AAC Cuts at: 2415 3369 2415 Size: 954 4825 KpnI G_GTAC'C Cuts at: 3104 3104 Size: 5779 MfeI C'AATT_G Cuts at: 3378 3378 Size: 5779 MluI A'CGCG_T Cuts at: 5655 5655 Size: 5779 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2027 4893 5077 1305 Size: 722 2866 184 2007 Fragments arranged by size: 2866 2007 722 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3957 Fragments arranged by size: 3957 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2187 3041 3737 4096 4255 518 Size: 648 1021 854 696 359 159 2042 Fragments arranged by size: 2042 1021 854 696 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2806 3030 3195 3872 5020 5265 1606 Size: 1200 224 165 677 1148 245 2120 Fragments arranged by size: 2120 1200 1148 677 245 224 165 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5227 NdeI CA'TA_TG Cuts at: 387 387 Size: 5779 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5779 NotI GC'GGCC_GC Cuts at: 3226 3226 Size: 5779 NsiI A_TGCA'T Cuts at: 2194 2194 Size: 5779 NspI r_CATG'y Cuts at: 1132 1229 2710 3137 4682 1132 Size: 97 1481 427 1545 2229 Fragments arranged by size: 2229 1545 1481 427 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5779 PciI A'CATG_T Cuts at: 4678 4678 Size: 5779 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5779 PfoI T'CCnGG_A Cuts at: 2035 2035 Size: 5779 PmeI GTTT'AAAC Cuts at: 3083 3083 Size: 5779 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3848 3880 5361 5393 3848 Size: 32 1481 32 4234 Fragments arranged by size: 4234 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5779 PsiI TTA'TAA Cuts at: 3349 3349 Size: 5779 PstI C_TGCA'G Cuts at: 830 2975 3131 830 Size: 2145 156 3478 Fragments arranged by size: 3478 2145 156 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5779 PvuII CAG'CTG Cuts at: 2806 3030 2806 Size: 224 5555 SalI G'TCGA_C Cuts at: 1936 3121 1936 Size: 1185 4594 SapI GCTCTTCn'nnn_ Cuts at: 2172 2172 Size: 5779 SbfI CC_TGCA'GG Cuts at: 3131 3131 Size: 5779 ScaI AGT'ACT Cuts at: 4027 5739 4027 Size: 1712 4067 SfcI C'TryA_G Cuts at: 826 1045 2642 2971 3127 4262 4943 5134 826 Size: 219 1597 329 156 1135 681 191 1471 Fragments arranged by size: 1597 1471 1135 681 329 219 191 156 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5779 SmaI CCC'GGG Cuts at: 1872 3106 1872 Size: 1234 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2341 3883 4784 5046 820 Size: 197 926 398 1542 901 262 1553 Fragments arranged by size: 1553 1542 926 901 398 262 197 SnaBI TAC'GTA Cuts at: 493 3570 493 Size: 3077 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5779 SphI G_CATG'C Cuts at: 1132 3137 1132 Size: 2005 3774 SspI AAT'ATT Cuts at: 5 52 2166 2596 3703 5 Size: 47 2114 430 1107 2081 Fragments arranged by size: 2114 2081 1107 430 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5779 StyI C'CwwG_G Cuts at: 513 1065 2310 3218 513 Size: 552 1245 908 3074 Fragments arranged by size: 3074 1245 908 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3806 3823 3976 4161 4606 325 Size: 1255 2226 17 153 185 445 1498 Fragments arranged by size: 2226 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2901 4025 5737 Size: 274 80 33 51 1443 924 1124 1712 Cuts at: 5737 96 Size: 138 Fragments arranged by size: 1712 1443 1124 924 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2062 2094 5739 5771 2062 Size: 32 3645 32 2070 Fragments arranged by size: 3645 2070 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5779 XbaI T'CTAG_A Cuts at: 3115 3115 Size: 5779 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5779 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5779 XmaI C'CCGG_G Cuts at: 1870 3104 1870 Size: 1234 4545 XmnI GAAnn'nnTTC Cuts at: 2447 2619 3908 2447 Size: 172 1289 4318 Fragments arranged by size: 4318 1289 172 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3587 276 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI