Restriction map for
pFN21AE3150
(Linear) (Six-base) MAPSORT of: KIEE3150.seq Check: 7159 from: 1 to: 1074
KIEE3150.orf
With 182 enzymes: SgfI *
July 3, 2012 17:23 ..
AccI GT'mk_AC
Cuts at: 0 849 1074
Size: 849 225
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 892 1074
Size: 631 261 182
Fragments arranged by size:
631 261 182
AgeI A'CCGG_T
Cuts at: 0 722 1074
Size: 722 352
AleI CACnn'nnGTG
Cuts at: 0 1036 1074
Size: 1036 38
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 200 410 1074
Size: 200 210 664
Fragments arranged by size:
664 210 200
BbsI GAAGACnn'nnnn_
Cuts at: 0 797 1074
Size: 797 277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 940 974 1074
Size: 940 34 100
Fragments arranged by size:
940 100 34
BfrBI ATG'CAT
Cuts at: 0 187 1074
Size: 187 887
BglII A'GATC_T
Cuts at: 0 25 510 1074
Size: 25 485 564
Fragments arranged by size:
564 485 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 399 1074
Size: 399 675
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 1074
Size: 42 1032
Bpu10I CC'TnA_GC
Cuts at: 0 526 1074
Size: 526 548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 1074
Size: 321 753
BsaBI GATnn'nnATC
Cuts at: 0 75 1074
Size: 75 999
BsaWI w'CCGG_w
Cuts at: 0 722 1074
Size: 722 352
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 569 1074
Size: 569 505
BseYI C'CCAG_C
Cuts at: 0 921 1074
Size: 921 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 987 1074
Size: 682 305 87
Fragments arranged by size:
682 305 87
BsiEI CG_ry'CG
Cuts at: 0 1013 1074
Size: 1013 61
BsmI GAATG_Cn'
Cuts at: 0 542 971 1074
Size: 542 429 103
Fragments arranged by size:
542 429 103
BsrFI r'CCGG_y
Cuts at: 0 722 1074
Size: 722 352
BstEII G'GTnAC_C
Cuts at: 0 762 1074
Size: 762 312
BstYI r'GATC_y
Cuts at: 0 25 257 510 642 1074
Size: 25 232 253 132 432
Fragments arranged by size:
432 253 232 132 25
Bsu36I CC'TnA_GG
Cuts at: 0 753 1074
Size: 753 321
BtsI GCAGTG_nn'
Cuts at: 0 946 963 1074
Size: 946 17 111
Fragments arranged by size:
946 111 17
EaeI y'GGCC_r
Cuts at: 0 1010 1074
Size: 1010 64
EagI C'GGCC_G
Cuts at: 0 1010 1074
Size: 1010 64
EarI CTCTTCn'nnn_
Cuts at: 0 61 167 1069 1074
Size: 61 106 902 5
Fragments arranged by size:
902 106 61 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 631 892 1074
Size: 42 589 261 182
Fragments arranged by size:
589 261 182 42
EcoRV GAT'ATC
Cuts at: 0 354 1074
Size: 354 720
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 459 1074
Size: 459 615
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 514 546 728 760 842 874
Size: 10 32 472 32 182 32 82 32
Cuts at: 874 1074
Size: 200
Fragments arranged by size:
472 200 182 82 32 32 32 32
10
HincII GTy'rAC
Cuts at: 0 410 1074
Size: 410 664
HindIII A'AGCT_T
Cuts at: 0 811 1074
Size: 811 263
HpaI GTT'AAC
Cuts at: 0 410 1074
Size: 410 664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 1074
Size: 22 1052
MslI CAynn'nnrTG
Cuts at: 0 182 1036 1074
Size: 182 854 38
Fragments arranged by size:
854 182 38
MspA1I CmG'CkG
Cuts at: 0 801 1025 1074
Size: 801 224 49
Fragments arranged by size:
801 224 49
NsiI A_TGCA'T
Cuts at: 0 189 1074
Size: 189 885
NspI r_CATG'y
Cuts at: 0 705 1074
Size: 705 369
PfoI T'CCnGG_A
Cuts at: 0 30 1074
Size: 30 1044
PstI C_TGCA'G
Cuts at: 0 970 1074
Size: 970 104
PvuII CAG'CTG
Cuts at: 0 801 1025 1074
Size: 801 224 49
Fragments arranged by size:
801 224 49
SapI GCTCTTCn'nnn_
Cuts at: 0 167 1074
Size: 167 907
SfcI C'TryA_G
Cuts at: 0 637 966 1074
Size: 637 329 108
Fragments arranged by size:
637 329 108
SmlI C'TyrA_G
Cuts at: 0 336 1074
Size: 336 738
SspI AAT'ATT
Cuts at: 0 161 591 1074
Size: 161 430 483
Fragments arranged by size:
483 430 161
StyI C'CwwG_G
Cuts at: 0 305 1074
Size: 305 769
TatI w'GTAC_w
Cuts at: 0 896 1074
Size: 896 178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 57 89 1074
Size: 57 32 985
Fragments arranged by size:
985 57 32
XmnI GAAnn'nnTTC
Cuts at: 0 442 614 1074
Size: 442 172 460
Fragments arranged by size:
460 442 172
Enzymes that do cut:
AccI AcuI AgeI AleI AlfI BbsI BcgI BfrBI
BglII BmrI BpmI Bpu10I BpuEI BsaBI BsaWI BseRI
BseYI BsgI BsiEI BsmI BsrFI BstEII BstYI Bsu36I
BtsI EaeI EagI EarI Eco57MI EcoRV FalI Hin4I
HincII HindIII HpaI MmeI MslI MspA1I NsiI NspI
PfoI PstI PvuII SapI SfcI SmlI SspI StyI
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BciVI BclI BglI BlpI Bme1580I BmgBI BmtI BplI
BsaI BsaAI BsaHI BsaXI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstZ17I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoICRI EcoNI EcoO109I EcoRI
FseI FspI FspAI HaeII KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
ZraI