(Circular) (Six-base) MAPSORT of: pFN21AE9399.seq Check: 4400 from: 1 to: 12850 pFN21AE9399 12850 bp With 182 enzymes: SgfI * August 18, 2017 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6179 6179 Size: 12850 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5952 9722 10660 278 Size: 53 83 186 936 4416 3770 938 2468 Fragments arranged by size: 4416 3770 2468 938 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5007 9741 10193 1937 Size: 3070 4734 452 4594 Fragments arranged by size: 4734 4594 3070 452 Acc65I G'GTAC_C Cuts at: 10171 10171 Size: 12850 AclI AA'CG_TT Cuts at: 1563 10149 10977 11350 1563 Size: 8586 828 373 3063 Fragments arranged by size: 8586 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3587 4001 4047 4557 4686 6518 6959 7935 Size: 1955 414 46 510 129 1832 441 976 Cuts at: 7935 10913 12297 12565 1632 Size: 2978 1384 268 1917 Fragments arranged by size: 2978 1955 1917 1832 1384 976 510 441 414 268 129 46 AfeI AGC'GCT Cuts at: 5813 6368 8155 10130 5813 Size: 555 1787 1975 8533 Fragments arranged by size: 8533 1975 1787 555 AflII C'TTAA_G Cuts at: 820 1017 9710 820 Size: 197 8693 3960 Fragments arranged by size: 8693 3960 197 AflIII A'CryG_T Cuts at: 1203 4612 4924 5553 6826 9570 11749 12726 1203 Size: 3409 312 629 1273 2744 2179 977 1327 Fragments arranged by size: 3409 2744 2179 1327 1273 977 629 312 AgeI A'CCGG_T Cuts at: 11681 11681 Size: 12850 AhdI GACnn_n'nnGTC Cuts at: 4939 11579 4939 Size: 6640 6210 AleI CACnn'nnGTG Cuts at: 1166 5588 5766 9966 1166 Size: 4422 178 4200 4050 Fragments arranged by size: 4422 4200 4050 178 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2710 4901 6083 6446 9957 10121 1184 Size: 1526 2191 1182 363 3511 164 3913 Fragments arranged by size: 3913 3511 2191 1526 1182 363 164 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4364 4396 6860 6892 4364 Size: 32 2464 32 10322 Fragments arranged by size: 10322 2464 32 32 AlwNI CAG_nnn'CTG Cuts at: 2581 2831 3085 4216 4225 4249 4799 4826 5357 Size: 250 254 1131 9 24 550 27 531 Cuts at: 5357 5976 6262 7017 7525 10593 12165 2581 Size: 619 286 755 508 3068 1572 3266 Fragments arranged by size: 3266 3068 1572 1131 755 619 550 531 508 286 254 250 27 24 9 ApaI G_GGCC'C Cuts at: 2312 2605 2820 3952 4982 5166 5196 5303 5603 Size: 293 215 1132 1030 184 30 107 300 Cuts at: 5603 5850 6229 6287 7928 8063 8314 8512 9057 Size: 247 379 58 1641 135 251 198 545 Cuts at: 9057 2312 Size: 6105 Fragments arranged by size: 6105 1641 1132 1030 545 379 300 293 251 247 215 198 184 135 107 58 30 ApaLI G'TGCA_C Cuts at: 5507 5986 6395 6747 7418 9559 10907 12063 5507 Size: 479 409 352 671 2141 1348 1156 6294 Fragments arranged by size: 6294 2141 1348 1156 671 479 409 352 ApoI r'AATT_y Cuts at: 1493 5753 7341 10159 10385 10537 12646 1493 Size: 4260 1588 2818 226 152 2109 1697 Fragments arranged by size: 4260 2818 2109 1697 1588 226 152 AseI AT'TA_AT Cuts at: 160 1032 11404 160 Size: 872 10372 1606 Fragments arranged by size: 10372 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12850 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2065 2186 2311 2451 2661 3459 Size: 73 93 29 121 125 140 210 798 Cuts at: 3459 3849 4083 4580 4650 5166 5195 5294 5328 Size: 390 234 497 70 516 29 99 34 Cuts at: 5328 5862 6906 7005 8062 8238 9339 9534 9987 Size: 534 1044 99 1057 176 1101 195 453 Cuts at: 9987 10175 1870 Size: 188 4545 Fragments arranged by size: 4545 1101 1057 1044 798 534 516 497 453 390 234 210 195 188 176 140 125 121 99 99 93 73 70 34 29 29 AvrII C'CTAG_G Cuts at: 8365 8365 Size: 12850 BamHI G'GATC_C Cuts at: 5786 6679 6706 10180 10547 5786 Size: 893 27 3474 367 8089 Fragments arranged by size: 8089 3474 893 367 27 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2081 2608 3476 4660 5421 Size: 325 217 627 294 527 868 1184 761 Cuts at: 5421 5880 6947 7335 7825 8419 9150 10121 10171 Size: 459 1067 388 490 594 731 971 50 Cuts at: 10171 11626 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1184 1067 971 868 761 731 627 594 527 490 459 388 325 294 217 50 BanII G_rGCy'C Cuts at: 1391 2187 2223 2312 2605 2643 2760 2820 2907 Size: 796 36 89 293 38 117 60 87 Cuts at: 2907 3059 3952 4024 4177 4469 4818 4982 5166 Size: 152 893 72 153 292 349 164 184 Cuts at: 5166 5196 5295 5303 5603 5850 6117 6229 6272 Size: 30 99 8 300 247 267 112 43 Cuts at: 6272 6287 6492 6933 7446 7498 7594 7928 8010 Size: 15 205 441 513 52 96 334 82 Cuts at: 8010 8063 8170 8314 8512 8553 9033 9057 9179 Size: 53 107 144 198 41 480 24 122 Cuts at: 9179 9451 9975 10062 10169 1391 Size: 272 524 87 107 4072 Fragments arranged by size: 4072 893 796 524 513 480 441 349 334 300 293 292 272 267 247 205 198 184 164 153 152 144 122 117 112 107 107 99 96 89 87 87 82 72 60 53 52 43 41 38 36 30 24 15 8 BbeI G_GCGC'C Cuts at: 2085 5425 5884 6951 8423 10125 2085 Size: 3340 459 1067 1472 1702 4810 Fragments arranged by size: 4810 3340 1702 1472 1067 459 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3534 3657 5084 6048 6132 6850 7036 Size: 971 1635 123 1427 964 84 718 186 Cuts at: 7036 7923 9722 928 Size: 887 1799 4056 Fragments arranged by size: 4056 1799 1635 1427 971 964 887 718 186 123 84 BbvCI CC'TCA_GC Cuts at: 3165 4109 6685 6980 7815 8220 3165 Size: 944 2576 295 835 405 7795 Fragments arranged by size: 7795 2576 944 835 405 295 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8219 8253 11041 11075 1903 Size: 34 6282 34 2788 34 3678 Fragments arranged by size: 6282 3678 2788 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4720 8923 10742 11952 4720 Size: 4203 1819 1210 5618 Fragments arranged by size: 5618 4203 1819 1210 BclI T'GATC_A Cuts at: 1548 2916 1548 Size: 1368 11482 BfrBI ATG'CAT Cuts at: 5825 9934 5825 Size: 4109 8741 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2868 2877 Size: 107 122 71 631 605 337 859 9 Cuts at: 2877 6205 7363 8036 8058 11461 136 Size: 3328 1158 673 22 3403 1525 Fragments arranged by size: 3403 3328 1525 1158 859 673 631 605 337 122 107 71 22 9 BglII A'GATC_T Cuts at: 3984 4509 12845 3984 Size: 525 8336 3989 Fragments arranged by size: 8336 3989 525 BlpI GC'TnA_GC Cuts at: 4071 4209 4463 6266 8795 10267 4071 Size: 138 254 1803 2529 1472 6654 Fragments arranged by size: 6654 2529 1803 1472 254 138 Bme1580I G_kGCm'C Cuts at: 1790 2312 2605 2820 3479 3607 3763 3952 4690 Size: 522 293 215 659 128 156 189 738 Cuts at: 4690 4982 5166 5196 5303 5511 5603 5850 5990 Size: 292 184 30 107 208 92 247 140 Cuts at: 5990 6229 6287 6399 6751 7006 7422 7608 7830 Size: 239 58 112 352 255 416 186 222 Cuts at: 7830 7928 8063 8252 8314 8512 9057 9563 10911 Size: 98 135 189 62 198 545 506 1348 Cuts at: 10911 12067 1790 Size: 1156 2573 Fragments arranged by size: 2573 1348 1156 738 659 545 522 506 416 352 293 292 255 247 239 222 215 208 198 189 189 186 184 156 140 135 128 112 107 98 92 62 58 30 BmgBI CAC'GTC Cuts at: 1318 5764 6829 9665 1318 Size: 4446 1065 2836 4503 Fragments arranged by size: 4503 4446 2836 1065 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2400 3302 3776 Size: 416 53 83 399 24 983 902 474 Cuts at: 3776 5953 6100 6298 8639 9139 10581 11539 442 Size: 2177 147 198 2341 500 1442 958 1753 Fragments arranged by size: 2341 2177 1753 1442 983 958 902 500 474 416 399 198 147 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12850 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3496 4588 5224 6133 7525 3496 Size: 1092 636 909 1392 8821 Fragments arranged by size: 8821 1392 1092 909 636 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3213 3385 4050 4161 4491 4848 5115 5714 Size: 1974 172 665 111 330 357 267 599 Cuts at: 5714 5822 5916 5982 6215 6239 6492 6657 6749 Size: 108 94 66 233 24 253 165 92 Cuts at: 6749 7318 7367 7409 7512 7551 8184 9044 9698 Size: 569 49 42 103 39 633 860 654 Cuts at: 9698 11510 1239 Size: 1812 2579 Fragments arranged by size: 2579 1974 1812 860 665 654 633 599 569 357 330 267 253 233 172 165 111 108 103 94 92 66 49 42 39 24 Bpu10I CC'TnA_GC Cuts at: 2754 3165 4035 4077 4109 6218 6685 6980 7500 Size: 411 870 42 32 2109 467 295 520 Cuts at: 7500 7815 7820 8220 8547 9650 2754 Size: 315 5 400 327 1103 5954 Fragments arranged by size: 5954 2109 1103 870 520 467 411 400 327 315 295 42 32 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4376 5393 6866 9158 10975 11840 12138 4376 Size: 1017 1473 2292 1817 865 298 5088 Fragments arranged by size: 5088 2292 1817 1473 1017 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2664 3685 6446 6644 7704 11513 882 Size: 617 1165 1021 2761 198 1060 3809 2219 Fragments arranged by size: 3809 2761 2219 1165 1060 1021 617 198 BsaAI yAC'GTr Cuts at: 493 1204 3396 3758 4925 10000 10641 493 Size: 711 2192 362 1167 5075 641 2702 Fragments arranged by size: 5075 2702 2192 1167 711 641 362 BsaBI GATnn'nnATC Cuts at: 9806 12844 9806 Size: 3038 9812 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2082 4739 5317 5422 Size: 53 83 186 936 549 2657 578 105 Cuts at: 5422 5881 5949 6531 6948 8420 9719 9924 10122 Size: 459 68 582 417 1472 1299 205 198 Cuts at: 10122 10657 11039 11674 275 Size: 535 382 635 1451 Fragments arranged by size: 2657 1472 1451 1299 936 635 582 578 549 535 459 417 382 205 198 186 105 83 68 53 BsaWI w'CCGG_w Cuts at: 3523 4530 5010 9088 9552 11283 11681 11955 12102 Size: 1007 480 4078 464 1731 398 274 147 Cuts at: 12102 12513 3523 Size: 411 3860 Fragments arranged by size: 4078 3860 1731 1007 480 464 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3259 3289 3469 3499 3994 Size: 30 165 30 1856 30 180 30 495 Cuts at: 3994 4024 4364 4394 6860 6890 7268 7298 7937 Size: 30 340 30 2466 30 378 30 639 Cuts at: 7937 7967 9290 9320 1178 Size: 30 1323 30 4708 Fragments arranged by size: 4708 2466 1856 1323 639 495 378 340 180 165 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2351 2378 2381 2481 2484 3182 3216 Size: 1162 3 27 3 100 3 698 34 Cuts at: 3216 3234 3264 3366 3371 3474 3601 3759 3855 Size: 18 30 102 5 103 127 158 96 Cuts at: 3855 3913 3996 3999 4002 4038 4379 4385 4499 Size: 58 83 3 3 36 341 6 114 Cuts at: 4499 5186 5213 5301 5375 5711 6458 6878 7415 Size: 687 27 88 74 336 747 420 537 Cuts at: 7415 7554 7788 8093 8096 8417 8711 9518 10070 Size: 139 234 305 3 321 294 807 552 Cuts at: 10070 1186 Size: 3966 Fragments arranged by size: 3966 1162 807 747 698 687 552 537 420 341 336 321 305 294 234 158 139 127 114 103 102 100 96 88 83 74 58 36 34 30 27 27 18 6 5 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2215 2304 2433 3388 3431 3500 3871 4242 5156 Size: 89 129 955 43 69 371 371 914 Cuts at: 5156 5718 5849 5991 6185 7723 7751 7996 8033 Size: 562 131 142 194 1538 28 245 37 Cuts at: 8033 8150 8470 8593 8618 9035 9450 9591 12053 Size: 117 320 123 25 417 415 141 2462 Cuts at: 12053 12549 2215 Size: 496 2516 Fragments arranged by size: 2516 2462 1538 955 914 562 496 417 415 371 371 320 245 194 142 141 131 129 123 117 89 69 43 37 28 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2594 4427 4949 6283 6314 8249 9665 Size: 573 858 1833 522 1334 31 1935 1416 Cuts at: 9665 1163 Size: 4348 Fragments arranged by size: 4348 1935 1833 1416 1334 858 573 522 31 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2075 2893 8863 10300 11061 12089 Size: 275 186 74 818 5970 1437 761 1028 Cuts at: 12089 1540 Size: 2301 Fragments arranged by size: 5970 2301 1437 1028 818 761 275 186 74 BsiHKAI G_wGCw'C Cuts at: 3401 5511 5583 5990 6255 6272 6399 6751 6933 Size: 2110 72 407 265 17 127 352 182 Cuts at: 6933 7197 7286 7304 7422 7498 9179 9563 9606 Size: 264 89 18 118 76 1681 384 43 Cuts at: 9606 10062 10911 10996 12067 3401 Size: 456 849 85 1071 4184 Fragments arranged by size: 4184 2110 1681 1071 849 456 407 384 352 265 264 182 127 118 89 85 76 72 43 18 17 BsmI GAATG_Cn' Cuts at: 6692 10361 10454 6692 Size: 3669 93 9088 Fragments arranged by size: 9088 3669 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3796 3796 Size: 12850 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2223 2312 2605 2643 2760 2820 Size: 399 397 36 89 293 38 117 60 Cuts at: 2820 2907 3059 3401 3479 3607 3763 3952 4024 Size: 87 152 342 78 128 156 189 72 Cuts at: 4024 4177 4469 4690 4818 4982 5166 5196 5295 Size: 153 292 221 128 164 184 30 99 Cuts at: 5295 5303 5511 5583 5603 5850 5990 6117 6229 Size: 8 208 72 20 247 140 127 112 Cuts at: 6229 6255 6272 6287 6399 6492 6751 6933 7006 Size: 26 17 15 112 93 259 182 73 Cuts at: 7006 7197 7286 7304 7422 7446 7498 7594 7608 Size: 191 89 18 118 24 52 96 14 Cuts at: 7608 7830 7928 8010 8063 8170 8252 8314 8512 Size: 222 98 82 53 107 82 62 198 Cuts at: 8512 8553 9033 9057 9179 9451 9563 9606 9975 Size: 41 480 24 122 272 112 43 369 Cuts at: 9975 10062 10169 10911 10996 12067 1391 Size: 87 107 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 480 399 397 369 342 293 292 272 259 247 222 221 208 198 191 189 184 182 164 156 153 152 140 128 128 127 122 118 117 112 112 112 107 107 99 98 96 93 89 89 87 87 85 82 82 78 73 72 72 62 60 53 52 43 41 38 36 30 26 24 24 20 18 17 15 14 8 BspEI T'CCGG_A Cuts at: 3523 9088 9552 12513 3523 Size: 5565 464 2961 3860 Fragments arranged by size: 5565 3860 2961 464 BspHI T'CATG_A Cuts at: 10739 12469 10739 Size: 1730 11120 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6179 8337 9138 10205 844 Size: 5335 2158 801 1067 3489 Fragments arranged by size: 5335 3489 2158 1067 801 BsrBI CCG'CTC Cuts at: 1394 1823 3448 3609 4567 5707 6222 9834 10737 Size: 429 1625 161 958 1140 515 3612 903 Cuts at: 10737 1394 Size: 3507 Fragments arranged by size: 3612 3507 1625 1140 958 903 515 429 161 BsrDI GCAATG_nn' Cuts at: 65 1248 9946 11345 11519 65 Size: 1183 8698 1399 174 1396 Fragments arranged by size: 8698 1399 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2070 2084 2238 2258 3414 5370 8862 Size: 110 260 14 154 20 1156 1956 3492 Cuts at: 8862 11494 11681 1700 Size: 2632 187 2869 Fragments arranged by size: 3492 2869 2632 1956 1156 260 187 154 110 20 14 BsrGI T'GTAC_A Cuts at: 96 6026 6165 6801 96 Size: 5930 139 636 6145 Fragments arranged by size: 6145 5930 636 139 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12850 BssSI C'ACGA_G Cuts at: 5642 10910 11922 5642 Size: 5268 1012 6570 Fragments arranged by size: 6570 5268 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4225 4234 5757 9601 9885 794 Size: 3431 9 1523 3844 284 3759 Fragments arranged by size: 3844 3759 3431 1523 284 9 BstBI TT'CG_AA Cuts at: 11654 11654 Size: 12850 BstEII G'GTnAC_C Cuts at: 1184 4135 1184 Size: 2951 9899 BstXI CCAn_nnnn'nTGG Cuts at: 5768 6280 7759 7785 7996 9676 5768 Size: 512 1479 26 211 1680 8942 Fragments arranged by size: 8942 1680 1479 512 211 26 BstYI r'GATC_y Cuts at: 1972 3038 3984 4509 5786 5889 6135 6679 6706 Size: 1066 946 525 1277 103 246 544 27 Cuts at: 6706 10180 10547 10932 10949 12401 12487 12499 12845 Size: 3474 367 385 17 1452 86 12 346 Cuts at: 12845 1972 Size: 1977 Fragments arranged by size: 3474 1977 1452 1277 1066 946 544 525 385 367 346 246 103 86 27 17 12 BstZ17I GTA'TAC Cuts at: 5008 5008 Size: 12850 Bsu36I CC'TnA_GG Cuts at: 3815 4573 5112 5358 7867 8322 8937 9609 3815 Size: 758 539 246 2509 455 615 672 7056 Fragments arranged by size: 7056 2509 758 672 615 539 455 246 BtgI C'CryG_G Cuts at: 513 1065 2004 2075 2101 2264 2863 2872 2887 Size: 552 939 71 26 163 599 9 15 Cuts at: 2887 3510 3540 4656 6951 7358 8040 8053 8682 Size: 623 30 1116 2295 407 682 13 629 Cuts at: 8682 8736 9523 9687 9757 513 Size: 54 787 164 70 3606 Fragments arranged by size: 3606 2295 1116 939 787 682 629 623 599 552 407 164 163 71 70 54 30 26 15 13 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 12111 Fragments arranged by size: 12111 683 56 BtsI GCAGTG_nn' Cuts at: 2350 4095 4859 5003 5345 6077 6287 6635 7535 Size: 1745 764 144 342 732 210 348 900 Cuts at: 7535 8806 8962 9268 10366 11160 11180 2350 Size: 1271 156 306 1098 794 20 4020 Fragments arranged by size: 4020 1745 1271 1098 900 794 764 732 348 342 306 210 156 144 20 ClaI AT'CG_AT Cuts at: 1554 4422 1554 Size: 2868 9982 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5764 5799 529 Size: 35 5200 35 7580 Fragments arranged by size: 7580 5200 35 35 DraI TTT'AAA Cuts at: 10154 10154 Size: 12850 DraIII CAC_nnn'GTG Cuts at: 3758 5736 6094 6306 9601 3758 Size: 1978 358 212 3295 7007 Fragments arranged by size: 7007 3295 1978 358 212 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5778 7040 7743 8128 11857 809 Size: 507 4462 1262 703 385 3729 1802 Fragments arranged by size: 4462 3729 1802 1262 703 507 385 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2072 2681 2890 Size: 54 1425 50 275 18 242 609 209 Cuts at: 2890 4491 6379 7355 7541 7986 8860 9429 9690 Size: 1601 1888 976 186 445 874 569 261 Cuts at: 9690 10297 11186 12815 8 Size: 607 889 1629 43 Fragments arranged by size: 1888 1629 1601 1425 976 889 874 609 607 569 445 275 261 242 209 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2072 2890 8860 10297 1537 Size: 275 260 818 5970 1437 4090 Fragments arranged by size: 5970 4090 1437 818 275 260 EarI CTCTTCn'nnn_ Cuts at: 1353 3025 3096 3135 3189 3401 3794 7268 7368 Size: 1672 71 39 54 212 393 3474 100 Cuts at: 7368 8013 9180 9627 10780 1353 Size: 645 1167 447 1153 3423 Fragments arranged by size: 3474 3423 1672 1167 1153 645 447 393 212 100 71 54 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2121 2190 2213 2234 3458 6866 Size: 1064 138 532 69 23 21 1224 3408 Cuts at: 6866 7103 8122 8741 11437 11811 11957 387 Size: 237 1019 619 2696 374 146 1280 Fragments arranged by size: 3408 2696 1280 1224 1064 1019 619 532 374 237 146 138 69 23 21 EcoICRI GAG'CTC Cuts at: 6270 6931 7496 9177 10060 6270 Size: 661 565 1681 883 9060 Fragments arranged by size: 9060 1681 883 661 565 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3213 3385 3587 4001 4047 4050 4161 Size: 393 1581 172 202 414 46 3 111 Cuts at: 4161 4491 4557 4686 4848 5115 5714 5822 5916 Size: 330 66 129 162 267 599 108 94 Cuts at: 5916 5982 6215 6239 6492 6518 6657 6749 6959 Size: 66 233 24 253 26 139 92 210 Cuts at: 6959 7318 7367 7409 7512 7551 7935 8184 9044 Size: 359 49 42 103 39 384 249 860 Cuts at: 9044 9698 10913 11510 12297 12565 1239 Size: 654 1215 597 787 268 1524 Fragments arranged by size: 1581 1524 1215 860 787 654 599 597 414 393 384 359 330 268 267 253 249 233 210 202 172 162 139 129 111 108 103 94 92 66 66 49 46 42 39 26 24 3 EcoNI CCTnn'n_nnAGG Cuts at: 2698 3928 4253 4593 4643 5429 5696 6245 7866 Size: 1230 325 340 50 786 267 549 1621 Cuts at: 7866 2698 Size: 7682 Fragments arranged by size: 7682 1621 1230 786 549 340 325 267 50 EcoO109I rG'GnC_Cy Cuts at: 2230 2308 2690 2816 2828 2835 3518 3933 3949 Size: 78 382 126 12 7 683 415 16 Cuts at: 3949 4523 4577 4598 4711 4796 4833 4941 4978 Size: 574 54 21 113 85 37 108 37 Cuts at: 4978 4979 5162 5163 5192 5299 5300 5569 5599 Size: 1 183 1 29 107 1 269 30 Cuts at: 5599 5600 5846 6214 6225 6226 6238 6259 6338 Size: 1 246 368 11 1 12 21 79 Cuts at: 6338 6733 7459 7629 8059 8287 8310 8326 8372 Size: 395 726 170 430 228 23 16 46 Cuts at: 8372 8508 8509 8714 9004 9054 9109 9220 2230 Size: 136 1 205 290 50 55 111 5860 Fragments arranged by size: 5860 726 683 574 430 415 395 382 368 290 269 246 228 205 183 170 136 126 113 111 108 107 85 79 78 55 54 50 46 37 37 30 29 23 21 21 16 16 12 12 11 7 1 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 7341 10159 10537 7341 Size: 2818 378 9654 Fragments arranged by size: 9654 2818 378 EcoRV GAT'ATC Cuts at: 5266 5266 Size: 12850 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 7060 7695 9580 7060 Size: 635 1885 10330 Fragments arranged by size: 10330 1885 635 FseI GG_CCGG'CC Cuts at: 2074 2262 3418 2074 Size: 188 1156 11506 Fragments arranged by size: 11506 1156 188 FspI TGC'GCA Cuts at: 4889 6076 6627 11356 4889 Size: 1187 551 4729 6383 Fragments arranged by size: 6383 4729 1187 551 FspAI rTGC'GCAy Cuts at: 4889 6076 4889 Size: 1187 11663 HaeII r_GCGC'y Cuts at: 1678 2085 2450 4858 5239 5425 5815 5884 6370 Size: 407 365 2408 381 186 390 69 486 Cuts at: 6370 6951 7324 7507 7560 8157 8423 10125 10132 Size: 581 373 183 53 597 266 1702 7 Cuts at: 10132 11997 1678 Size: 1865 2531 Fragments arranged by size: 2531 2408 1865 1702 597 581 486 407 390 381 373 365 266 186 183 69 53 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2339 2371 3259 3291 3795 Size: 32 135 32 767 32 888 32 504 Cuts at: 3795 3827 4192 4224 4512 4544 4715 4718 4747 Size: 32 365 32 288 32 171 3 29 Cuts at: 4747 4750 4925 4957 6761 6793 7937 7969 8069 Size: 3 175 32 1804 32 1144 32 100 Cuts at: 8069 8101 8318 8350 9179 9211 9738 9770 11491 Size: 32 217 32 829 32 527 32 1721 Cuts at: 11491 11523 11565 11597 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1804 1721 1144 888 829 767 527 504 365 288 217 175 171 135 100 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2996 4951 6832 8587 9657 9843 Size: 988 281 1058 1955 1881 1755 1070 186 Cuts at: 9843 10194 10440 669 Size: 351 246 3079 Fragments arranged by size: 3079 1955 1881 1755 1070 1058 988 351 281 246 186 HindIII A'AGCT_T Cuts at: 748 748 Size: 12850 HpaI GTT'AAC Cuts at: 10440 10440 Size: 12850 KasI G'GCGC_C Cuts at: 2081 5421 5880 6947 8419 10121 2081 Size: 3340 459 1067 1472 1702 4810 Fragments arranged by size: 4810 3340 1702 1472 1067 459 KpnI G_GTAC'C Cuts at: 10175 10175 Size: 12850 MfeI C'AATT_G Cuts at: 2957 10449 2957 Size: 7492 5358 MluI A'CGCG_T Cuts at: 12726 12726 Size: 12850 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2382 3016 3518 4539 5300 5882 8722 11964 Size: 1077 634 502 1021 761 582 2840 3242 Cuts at: 11964 12148 1305 Size: 184 2007 Fragments arranged by size: 3242 2840 2007 1077 1021 761 634 582 502 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6381 9692 10 Size: 54 1425 343 4549 3311 3168 Fragments arranged by size: 4549 3311 3168 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2388 5588 5766 6428 6621 7190 7783 Size: 648 1222 3200 178 662 193 569 593 Cuts at: 7783 7946 9411 9692 9939 9966 9998 10808 11167 Size: 163 1465 281 247 27 32 810 359 Cuts at: 11167 11326 518 Size: 159 2042 Fragments arranged by size: 3200 2042 1465 1222 810 662 648 593 569 359 281 247 193 178 163 159 32 27 MspA1I CmG'CkG Cuts at: 1606 2077 2103 2266 2275 2735 2799 2889 3182 Size: 471 26 163 9 460 64 90 293 Cuts at: 3182 3439 4070 4282 4357 4462 6204 8033 8185 Size: 257 631 212 75 105 1742 1829 152 Cuts at: 8185 8474 9035 10266 10943 12091 12336 1606 Size: 289 561 1231 677 1148 245 2120 Fragments arranged by size: 2120 1829 1742 1231 1148 677 631 561 471 460 293 289 257 245 212 163 152 105 90 75 64 26 9 NaeI GCC'GGC Cuts at: 2072 2086 2240 2260 3416 5372 2072 Size: 14 154 20 1156 1956 9550 Fragments arranged by size: 9550 1956 1156 154 20 14 NarI GG'CG_CC Cuts at: 2082 5422 5881 6948 8420 10122 2082 Size: 3340 459 1067 1472 1702 4810 Fragments arranged by size: 4810 3340 1702 1472 1067 459 NcoI C'CATG_G Cuts at: 513 1065 2004 4656 7358 8040 9523 9687 513 Size: 552 939 2652 2702 682 1483 164 3676 Fragments arranged by size: 3676 2702 2652 1483 939 682 552 164 NdeI CA'TA_TG Cuts at: 387 387 Size: 12850 NgoMIV G'CCGG_C Cuts at: 2070 2084 2238 2258 3414 5370 2070 Size: 14 154 20 1156 1956 9550 Fragments arranged by size: 9550 1956 1156 154 20 14 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12850 NotI GC'GGCC_GC Cuts at: 10297 10297 Size: 12850 NsiI A_TGCA'T Cuts at: 5827 9936 5827 Size: 4109 8741 NspI r_CATG'y Cuts at: 1132 1229 2637 4616 6101 6896 9350 9574 10208 Size: 97 1408 1979 1485 795 2454 224 634 Cuts at: 10208 11753 1132 Size: 1545 2229 Fragments arranged by size: 2454 2229 1979 1545 1485 1408 795 634 224 97 PasI CC'CwG_GG Cuts at: 1348 4639 4799 5091 5189 7222 8504 8513 1348 Size: 3291 160 292 98 2033 1282 9 5685 Fragments arranged by size: 5685 3291 2033 1282 292 160 98 9 PciI A'CATG_T Cuts at: 4612 9570 11749 4612 Size: 4958 2179 5713 Fragments arranged by size: 5713 4958 2179 PflMI CCAn_nnn'nTGG Cuts at: 1108 2831 5475 5647 6549 8697 8951 1108 Size: 1723 2644 172 902 2148 254 5007 Fragments arranged by size: 5007 2644 2148 1723 902 254 172 PfoI T'CCnGG_A Cuts at: 2824 3401 3926 4698 5799 7026 7068 7622 8013 Size: 577 525 772 1101 1227 42 554 391 Cuts at: 8013 8696 9060 2824 Size: 683 364 6614 Fragments arranged by size: 6614 1227 1101 772 683 577 554 525 391 364 42 PmeI GTTT'AAAC Cuts at: 10154 10154 Size: 12850 PmlI CAC'GTG Cuts at: 3758 10000 3758 Size: 6242 6608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4364 4396 5636 5668 6860 6892 9257 9289 10919 Size: 32 1240 32 1192 32 2365 32 1630 Cuts at: 10919 10951 12432 12464 4364 Size: 32 1481 32 4750 Fragments arranged by size: 4750 2365 1630 1481 1240 1192 32 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2828 2835 4577 4598 4711 4833 4941 6338 6733 Size: 7 1742 21 113 122 108 1397 395 Cuts at: 6733 7459 8287 8326 8372 9004 9109 2828 Size: 726 828 39 46 632 105 6569 Fragments arranged by size: 6569 1742 1397 828 726 632 395 122 113 108 105 46 39 21 7 PshAI GACnn'nnGTC Cuts at: 1613 2959 6738 6827 1613 Size: 1346 3779 89 7636 Fragments arranged by size: 7636 3779 1346 89 PsiI TTA'TAA Cuts at: 10420 10420 Size: 12850 PspOMI G'GGCC_C Cuts at: 2308 2601 2816 3948 4978 5162 5192 5299 5599 Size: 293 215 1132 1030 184 30 107 300 Cuts at: 5599 5846 6225 6283 7924 8059 8310 8508 9053 Size: 247 379 58 1641 135 251 198 545 Cuts at: 9053 2308 Size: 6105 Fragments arranged by size: 6105 1641 1132 1030 545 379 300 293 251 247 215 198 184 135 107 58 30 PstI C_TGCA'G Cuts at: 830 2699 4221 4230 4287 5220 5394 7399 8302 Size: 1869 1522 9 57 933 174 2005 903 Cuts at: 8302 10202 830 Size: 1900 3478 Fragments arranged by size: 3478 2005 1900 1869 1522 933 903 174 57 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12850 PvuII CAG'CTG Cuts at: 2735 2799 3182 4070 4282 8185 8474 2735 Size: 64 383 888 212 3903 289 7111 Fragments arranged by size: 7111 3903 888 383 289 212 64 RsrII CG'GwC_CG Cuts at: 6176 6460 6176 Size: 284 12566 SacI G_AGCT'C Cuts at: 6272 6933 7498 9179 10062 6272 Size: 661 565 1681 883 9060 Fragments arranged by size: 9060 1681 883 661 565 SacII CC_GC'GG Cuts at: 2078 2104 2267 2890 2078 Size: 26 163 623 12038 Fragments arranged by size: 12038 623 163 26 SalI G'TCGA_C Cuts at: 1936 10192 1936 Size: 8256 4594 SanDI GG'GwC_CC Cuts at: 2835 4833 2835 Size: 1998 10852 SapI GCTCTTCn'nnn_ Cuts at: 7268 7368 9180 7268 Size: 100 1812 10938 Fragments arranged by size: 10938 1812 100 SbfI CC_TGCA'GG Cuts at: 2699 4230 10202 2699 Size: 1531 5972 5347 Fragments arranged by size: 5972 5347 1531 ScaI AGT'ACT Cuts at: 6548 9815 11098 12810 6548 Size: 3267 1283 1712 6588 Fragments arranged by size: 6588 3267 1712 1283 SexAI A'CCwGG_T Cuts at: 5732 5732 Size: 12850 SfcI C'TryA_G Cuts at: 826 1045 2695 3159 4217 4226 4283 5216 5390 Size: 219 1650 464 1058 9 57 933 174 Cuts at: 5390 7395 8298 9742 10198 11333 12014 12205 826 Size: 2005 903 1444 456 1135 681 191 1471 Fragments arranged by size: 2005 1650 1471 1444 1135 1058 933 903 681 464 456 219 191 174 57 9 SfiI GGCCn_nnn'nGGCC Cuts at: 7363 7363 Size: 12850 SfoI GGC'GCC Cuts at: 2083 5423 5882 6949 8421 10123 2083 Size: 3340 459 1067 1472 1702 4810 Fragments arranged by size: 4810 3340 1702 1472 1067 459 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12850 SgrAI Cr'CCGG_yG Cuts at: 1700 2084 1700 Size: 384 12466 SmaI CCC'GGG Cuts at: 1872 2038 2067 2188 3851 4652 5168 5296 5864 Size: 166 29 121 1663 801 516 128 568 Cuts at: 5864 7007 8064 10177 1872 Size: 1143 1057 2113 4545 Fragments arranged by size: 4545 2113 1663 1143 1057 801 568 516 166 128 121 29 SmlI C'TyrA_G Cuts at: 820 1017 1943 4391 5408 6881 9137 9710 10954 Size: 197 926 2448 1017 1473 2256 573 1244 Cuts at: 10954 11855 12117 820 Size: 901 262 1553 Fragments arranged by size: 2448 2256 1553 1473 1244 1017 926 901 573 262 197 SnaBI TAC'GTA Cuts at: 493 10641 493 Size: 10148 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 12850 SphI G_CATG'C Cuts at: 1132 6101 6896 9350 10208 1132 Size: 4969 795 2454 858 3774 Fragments arranged by size: 4969 3774 2454 858 795 SrfI GCCC'GGGC Cuts at: 4652 7007 4652 Size: 2355 10495 SspI AAT'ATT Cuts at: 5 52 10774 5 Size: 47 10722 2081 Fragments arranged by size: 10722 2081 47 StuI AGG'CCT Cuts at: 1517 4232 5117 8457 8921 9768 1517 Size: 2715 885 3340 464 847 4599 Fragments arranged by size: 4599 3340 2715 885 847 464 StyI C'CwwG_G Cuts at: 513 1065 2004 2964 4656 6273 6780 7358 7632 Size: 552 939 960 1692 1617 507 578 274 Cuts at: 7632 8040 8290 8365 8452 8533 9523 9687 10289 Size: 408 250 75 87 81 990 164 602 Cuts at: 10289 513 Size: 3074 Fragments arranged by size: 3074 1692 1617 990 960 939 602 578 552 507 408 274 250 164 87 81 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5202 5697 6005 6082 6317 6348 9725 Size: 1255 3622 495 308 77 235 31 3377 Cuts at: 9725 10877 10894 11047 11232 11677 325 Size: 1152 17 153 185 445 1498 Fragments arranged by size: 3622 3377 1498 1255 1152 495 445 308 235 185 153 77 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6026 6165 6546 Size: 274 80 33 51 1443 4049 139 381 Cuts at: 6546 6801 7293 9813 11096 12808 96 Size: 255 492 2520 1283 1712 138 Fragments arranged by size: 4049 2520 1712 1443 1283 492 381 274 255 139 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2533 2565 3469 3501 5939 5971 9290 9322 12810 Size: 32 904 32 2438 32 3319 32 3488 Cuts at: 12810 12842 2533 Size: 32 2541 Fragments arranged by size: 3488 3319 2541 2438 904 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2289 4908 1316 Size: 973 2619 9258 Fragments arranged by size: 9258 2619 973 XbaI T'CTAG_A Cuts at: 10186 10186 Size: 12850 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3032 5797 5940 7522 9113 9730 3032 Size: 2765 143 1582 1591 617 6152 Fragments arranged by size: 6152 2765 1591 1582 617 143 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 12850 XmaI C'CCGG_G Cuts at: 1870 2036 2065 2186 3849 4650 5166 5294 5862 Size: 166 29 121 1663 801 516 128 568 Cuts at: 5862 7005 8062 10175 1870 Size: 1143 1057 2113 4545 Fragments arranged by size: 4545 2113 1663 1143 1057 801 568 516 166 128 121 29 XmnI GAAnn'nnTTC Cuts at: 4379 7043 10979 4379 Size: 2664 3936 6250 Fragments arranged by size: 6250 3936 2664 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5950 9720 10658 276 Size: 53 83 186 936 4416 3770 938 2468 Fragments arranged by size: 4416 3770 2468 938 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BsiWI NruI PacI PspXI PsrI SwaI