################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstF5I BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MseI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 683 TCCGGA T^CCGGA AcsI 514 GAATTC [GA]^AATT[CT] ApoI 514 GAATTC [GA]^AATT[CT] AvaII 309 GGACC G^G[AT]CC BalI 623 TGGCCA TGG^CCA BamHI 233 GGATCC G^GATCC BanII 100 GGGCTC G[GA]GC[CT]^C BciVI 273 GGATAC GTATCC(6/5) BfaI 121 CTAG C^TAG BmyI 100 GGGCTC G[AGT]GC[ACT]^C BsaAI 596 TACGTA [CT]AC^GT[GA] BsaWI 683 TCCGGA [AT]^CCGG[AT] BseAI 683 TCCGGA T^CCGGA BsmAI 535 GAGAC GTCTC(1/5) Bsp1286I 100 GGGCTC G[AGT]GC[ACT]^C BspEI 683 TCCGGA T^CCGGA BspHI 198 TCATGA T^CATGA BssKI 30 CCAGG ^CC[ACGT]GG BstEII 480 GGTCACC G^GT[ACGT]ACC BstNI 30 CCAGG CC^[AT]GG BstPI 480 GGTCACC G^GT[ACGT]ACC BstYI 233 GGATCC [GA]^GATC[CT] Cac8I 252 GCCTGC GC[ACGT]^[ACGT]GC DraII 36 AGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 626 CCATGG C^C[GA][CT]GG EaeI 623 TGGCCA [CT]^GGCC[GA] EcoO109I 36 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 480 GGTCACC G^GT[ACGT]ACC EcoRI 514 GAATTC G^AATTC EcoRII 30 CCAGG ^CC[AT]GG HapII 684 CCGG C^CGG HpaII 684 CCGG C^CGG MaeI 121 CTAG C^TAG MflI 233 GGATCC [GA]^GATC[CT] MluNI 623 TGGCCA TGG^CCA MroI 683 TCCGGA T^CCGGA MscI 623 TGGCCA TGG^CCA MslI 159 CATGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 684 CCGG C^CGG MvaI 30 CCAGG CC^[AT]GG NcoI 626 CCATGG C^CATGG PleI 410 GAGTC GAGTC(4/5) RcaI 198 TCATGA T^CATGA ScaI 618 AGTACT AGT^ACT ScrFI 30 CCAGG CC^[ACGT]GG SfcI 84 CTGTAG C^T[GA][CT]AG SnaBI 596 TACGTA TAC^GTA SpeI 120 ACTAGT A^CTAGT Tsp45I 481 GTCAC ^GT[GC]AC Tsp509I 515 AATT ^AATT TspRI 654 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 41 CCAAGAGGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 233 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AvaI 19 CTCGGG C^[CT]CG[GA]G 509 CCCGAG BsaBI 196 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 228 GATCAGGATC BsiYI 581 CCCTTGTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 582 CCTTGTACGGG BslI 581 CCCTTGTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 582 CCTTGTACGGG BsmFI 308 GGGAC GGGAC(10/14) 524 GTCCC BsoBI 19 CTCGGG C^[CT]CG[GA]G 509 CCCGAG DdeI 472 CTAAG C^T[ACGT]AG 641 CTAAG EarI 150 GAAGAG CTCTTC(1/4) 663 GAAGAG FauI 94 CCCGC CCCGC(4/6) 306 GCGGG HphI 482 TCACC GGTGA(8/7) 676 TCACC Ksp632I 150 GAAGAG CTCTTC(1/4) 663 GAAGAG MaeII 417 ACGT A^CGT 597 ACGT MamI 196 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 228 GATCAGGATC PvuII 278 CAGCTG CAG^CTG 519 CAGCTG SfaNI 126 GATGC GCATC(5/9) 217 GATGC TaqI 366 TCGA T^CGA 408 TCGA TthHB8I 366 TCGA T^CGA 408 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BsaJI BsrI Cfr13I DpnII EcoT14I Fnu4HI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MspA1I MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I StyI TfiI TseI