(Circular) (Six-base) MAPSORT of: pFN21AE4028.seq Check: 4903 from: 1 to: 5413 pFN21AE4028 5413 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3223 278 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2756 1937 Size: 819 4594 Acc65I G'GTAC_C Cuts at: 2734 2734 Size: 5413 AclI AA'CG_TT Cuts at: 1563 3540 3913 1563 Size: 1977 373 3063 Fragments arranged by size: 3063 1977 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3476 4860 5128 1632 Size: 1844 1384 268 1917 Fragments arranged by size: 1917 1844 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5216 AflIII A'CryG_T Cuts at: 1203 4312 5289 1203 Size: 3109 977 1327 Fragments arranged by size: 3109 1327 977 AgeI A'CCGG_T Cuts at: 4244 4244 Size: 5413 AhdI GACnn_n'nnGTC Cuts at: 4142 4142 Size: 5413 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5413 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5413 AlwNI CAG_nnn'CTG Cuts at: 2711 3156 4728 2711 Size: 445 1572 3396 Fragments arranged by size: 3396 1572 445 ApaLI G'TGCA_C Cuts at: 3470 4626 3470 Size: 1156 4257 ApoI r'AATT_y Cuts at: 1493 2519 2722 2948 3100 5209 1493 Size: 1026 203 226 152 2109 1697 Fragments arranged by size: 2109 1697 1026 226 203 152 AseI AT'TA_AT Cuts at: 160 1032 3967 160 Size: 872 2935 1606 Fragments arranged by size: 2935 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 AvaI C'yCGr_G Cuts at: 1870 1943 2024 2514 2738 1870 Size: 73 81 490 224 4545 Fragments arranged by size: 4545 490 224 81 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2424 2457 2424 Size: 33 5380 BamHI G'GATC_C Cuts at: 2238 2743 3110 2238 Size: 505 367 4541 Fragments arranged by size: 4541 505 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2734 4189 618 Size: 325 217 627 947 1455 1842 Fragments arranged by size: 1842 1455 947 627 325 217 BanII G_rGCy'C Cuts at: 1391 2109 2732 1391 Size: 718 623 4072 Fragments arranged by size: 4072 718 623 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4442 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3604 3638 1903 Size: 34 1667 34 3678 Fragments arranged by size: 3678 1667 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2272 3305 4515 2272 Size: 1033 1210 3170 Fragments arranged by size: 3170 1210 1033 BclI T'GATC_A Cuts at: 1548 1548 Size: 5413 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4024 136 Size: 107 122 71 631 605 2352 1525 Fragments arranged by size: 2352 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5408 5408 Size: 5413 BlpI GC'TnA_GC Cuts at: 2830 2830 Size: 5413 Bme1580I G_kGCm'C Cuts at: 1790 3474 4630 1790 Size: 1684 1156 2573 Fragments arranged by size: 2573 1684 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5413 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2069 3144 4102 Size: 416 53 83 399 24 652 1075 958 Cuts at: 4102 442 Size: 1753 Fragments arranged by size: 1753 1075 958 652 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5413 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4073 1239 Size: 2834 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3538 4403 4701 3538 Size: 865 298 4250 Fragments arranged by size: 4250 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4076 882 Size: 617 2577 2219 Fragments arranged by size: 2577 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2603 3204 493 Size: 711 1399 601 2702 Fragments arranged by size: 2702 1399 711 601 BsaBI GATnn'nnATC Cuts at: 2205 2237 5407 2205 Size: 32 3170 2211 Fragments arranged by size: 3170 2211 32 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3220 3602 4237 275 Size: 53 83 186 936 1687 382 635 1451 Fragments arranged by size: 1687 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2688 3846 4244 4518 4665 5076 2688 Size: 1158 398 274 147 411 3025 Fragments arranged by size: 3025 1158 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5188 Fragments arranged by size: 5188 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5413 BseYI C'CCAG_C Cuts at: 2102 2134 4616 5112 2102 Size: 32 2482 496 2403 Fragments arranged by size: 2482 2403 496 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4840 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2863 3624 4652 1540 Size: 275 186 862 761 1028 2301 Fragments arranged by size: 2301 1028 862 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3474 3559 4630 3474 Size: 85 1071 4257 Fragments arranged by size: 4257 1071 85 BsmI GAATG_Cn' Cuts at: 2924 3017 2924 Size: 93 5320 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2732 3474 3559 4630 1391 Size: 399 319 623 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 623 399 319 85 BspEI T'CCGG_A Cuts at: 2688 5076 2688 Size: 2388 3025 BspHI T'CATG_A Cuts at: 2203 3302 5032 2203 Size: 1099 1730 2584 Fragments arranged by size: 2584 1730 1099 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2768 844 Size: 1924 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3300 1394 Size: 429 1477 3507 Fragments arranged by size: 3507 1477 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3908 4082 65 Size: 1183 2660 174 1396 Fragments arranged by size: 2660 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4057 4244 1700 Size: 110 2247 187 2869 Fragments arranged by size: 2869 2247 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5413 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5413 BssSI C'ACGA_G Cuts at: 3473 4485 3473 Size: 1012 4401 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5413 BstBI TT'CG_AA Cuts at: 4217 4217 Size: 5413 BstEII G'GTnAC_C Cuts at: 1184 2485 1184 Size: 1301 4112 BstYI r'GATC_y Cuts at: 1972 2238 2743 3110 3495 3512 4964 5050 5062 Size: 266 505 367 385 17 1452 86 12 Cuts at: 5062 5408 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 505 385 367 346 266 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2631 513 Size: 552 939 627 3295 Fragments arranged by size: 3295 939 627 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4674 Fragments arranged by size: 4674 683 56 BtsI GCAGTG_nn' Cuts at: 2929 3723 3743 2929 Size: 794 20 4599 Fragments arranged by size: 4599 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5413 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5378 DraI TTT'AAA Cuts at: 2717 2717 Size: 5413 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4420 809 Size: 507 3104 1802 Fragments arranged by size: 3104 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2628 2860 3749 Size: 54 1425 50 275 18 798 232 889 Cuts at: 3749 5378 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 798 275 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2860 1537 Size: 275 1048 4090 Fragments arranged by size: 4090 1048 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2150 2663 3343 1353 Size: 797 513 680 3423 Fragments arranged by size: 3423 797 680 513 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4000 4374 4520 387 Size: 1064 138 2411 374 146 1280 Fragments arranged by size: 2411 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3476 4073 4860 5128 1239 Size: 393 1844 597 787 268 1524 Fragments arranged by size: 1844 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2042 2042 Size: 5413 EcoRI G'AATT_C Cuts at: 2519 2722 3100 2519 Size: 203 378 4832 Fragments arranged by size: 4832 378 203 FspI TGC'GCA Cuts at: 3919 3919 Size: 5413 HaeII r_GCGC'y Cuts at: 1678 4560 1678 Size: 2882 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2627 2659 4054 4086 4128 Size: 32 135 32 1055 32 1395 32 42 Cuts at: 4128 4160 1373 Size: 32 2626 Fragments arranged by size: 2626 1395 1055 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2757 3003 669 Size: 988 281 819 246 3079 Fragments arranged by size: 3079 988 819 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5413 HpaI GTT'AAC Cuts at: 3003 3003 Size: 5413 KpnI G_GTAC'C Cuts at: 2738 2738 Size: 5413 MfeI C'AATT_G Cuts at: 3012 3012 Size: 5413 MluI A'CGCG_T Cuts at: 5289 5289 Size: 5413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2443 4527 4711 1305 Size: 1138 2084 184 2007 Fragments arranged by size: 2084 2007 1138 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2630 10 Size: 54 1425 343 798 2793 Fragments arranged by size: 2793 1425 798 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 3371 3730 3889 518 Size: 648 1002 1203 359 159 2042 Fragments arranged by size: 2042 1203 1002 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2102 2285 2526 2829 3506 4654 4899 1606 Size: 496 183 241 303 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 496 303 245 241 183 NcoI C'CATG_G Cuts at: 513 1065 2004 2631 513 Size: 552 939 627 3295 Fragments arranged by size: 3295 939 627 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5413 NotI GC'GGCC_GC Cuts at: 2860 2860 Size: 5413 NspI r_CATG'y Cuts at: 1132 1229 2771 4316 1132 Size: 97 1542 1545 2229 Fragments arranged by size: 2229 1545 1542 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5413 PciI A'CATG_T Cuts at: 4312 4312 Size: 5413 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5413 PmeI GTTT'AAAC Cuts at: 2717 2717 Size: 5413 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3514 4995 5027 3482 Size: 32 1481 32 3868 Fragments arranged by size: 3868 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5413 PsiI TTA'TAA Cuts at: 2229 2983 2229 Size: 754 4659 PstI C_TGCA'G Cuts at: 830 2765 830 Size: 1935 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5413 PvuII CAG'CTG Cuts at: 2285 2526 2285 Size: 241 5172 SalI G'TCGA_C Cuts at: 1936 2755 1936 Size: 819 4594 SbfI CC_TGCA'GG Cuts at: 2765 2765 Size: 5413 ScaI AGT'ACT Cuts at: 2625 3661 5373 2625 Size: 1036 1712 2665 Fragments arranged by size: 2665 1712 1036 SfcI C'TryA_G Cuts at: 826 1045 2089 2761 3896 4577 4768 826 Size: 219 1044 672 1135 681 191 1471 Fragments arranged by size: 1471 1135 1044 681 672 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5413 SmaI CCC'GGG Cuts at: 1872 2740 1872 Size: 868 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3517 4418 4680 820 Size: 197 926 1574 901 262 1553 Fragments arranged by size: 1574 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2603 3204 493 Size: 2110 601 2702 Fragments arranged by size: 2702 2110 601 SpeI A'CTAG_T Cuts at: 152 2125 152 Size: 1973 3440 SphI G_CATG'C Cuts at: 1132 2771 1132 Size: 1639 3774 SspI AAT'ATT Cuts at: 5 52 3337 5 Size: 47 3285 2081 Fragments arranged by size: 3285 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5413 StyI C'CwwG_G Cuts at: 513 1065 2004 2490 2508 2631 2852 513 Size: 552 939 486 18 123 221 3074 Fragments arranged by size: 3074 939 552 486 221 123 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2126 3440 3457 3610 3795 4240 325 Size: 1255 546 1314 17 153 185 445 1498 Fragments arranged by size: 1498 1314 1255 546 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 2623 3659 Size: 274 80 33 51 1443 141 505 1036 Cuts at: 3659 5371 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1036 505 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5373 5405 5373 Size: 32 5381 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5413 XbaI T'CTAG_A Cuts at: 2749 2749 Size: 5413 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2053 2053 Size: 5413 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5413 XmaI C'CCGG_G Cuts at: 1870 2738 1870 Size: 868 4545 XmnI GAAnn'nnTTC Cuts at: 3542 3542 Size: 5413 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3221 276 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI