################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BanII BbrPI BbvCI BciVI BfrI BglI BlnI BsaAI BsaBI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FspI HgaI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp509I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 220 GACGTC GACGT^C AccI 1465 GTCGAC GT^[AC][GT]AC AccII 1526 CGCG CG^CG AfaI 993 GTAC GT^AC AflIII 1515 ACATGT A^C[GA][CT]GT AgeI 390 ACCGGT A^CCGGT Aor51HI 209 AGCGCT AGC^GCT Asp700I 1188 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 410 GGCGCC GGCGC^C BbsI 1219 GTCTTC GAAGAC(2/6) BcgI 1305 CGATGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 515 TGATCA T^GATCA BfaI 884 CTAG C^TAG BglII 1400 AGATCT A^GATCT BpuAI 1219 GTCTTC GAAGAC(2/6) BsaI 1120 GAGACC GGTCTC(1/5) BsaWI 390 ACCGGT [AT]^CCGG[AT] BsmI 423 GAATGC GAATGC(1/-1) BspHI 669 TCATGA T^CATGA BspLU11I 1515 ACATGT A^CATGT BssSI 655 CTCGTG CACGAG(-5/-1) BstUI 1526 CGCG CG^CG DsaI 133 CCATGG C^C[GA][CT]GG EarI 1408 CTCTTC CTCTTC(1/4) Eco47III 209 AGCGCT AGC^GCT EcoT14I 133 CCATGG C^C[AT][AT]GG FbaI 515 TGATCA T^GATCA FseI 319 GGCCGGCC GGCCGG^CC HinfI 382 GACTC G^A[ACGT]TC KasI 410 GGCGCC G^GCGCC Ksp632I 1408 CTCTTC CTCTTC(1/4) MaeI 884 CTAG C^TAG MseI 1319 TTAA T^TAA MslI 246 CACGCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 1526 CGCG CG^CG NarI 410 GGCGCC GG^CGCC NcoI 133 CCATGG C^CATGG PflFI 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 974 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 390 ACCGGT A^CCGGT PleI 382 GACTC GAGTC(4/5) PvuII 1090 CAGCTG CAG^CTG RcaI 669 TCATGA T^CATGA RsaI 993 GTAC GT^AC SalI 1465 GTCGAC G^TCGAC SfoI 410 GGCGCC GGC^GCC StyI 133 CCATGG C^C[AT][AT]GG Tru9I 1319 TTAA T^TAA Tth111I 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 974 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1188 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 220 GACGTC G[GA]^CG[CT]C 410 GGCGCC AlwI 231 GATCC GGATC(4/5) 1026 GATCC AspHI 79 GTGCTC G[AT]GC[AT]^C 808 GAGCAC AvaI 98 CTCGGG C^[CT]CG[GA]G 151 CTCGGG BlpI 261 GCTGAGC GC^T[ACGT]AGC 648 GCTCAGC Bpu1102I 261 GCTGAGC GC^T[ACGT]AGC 648 GCTCAGC BsaHI 220 GACGTC G[GA]^CG[CT]C 410 GGCGCC BsiHKAI 79 GTGCTC G[AT]GC[AT]^C 808 GAGCAC BsmAI 682 GAGAC GTCTC(1/5) 1120 GAGAC BsoBI 98 CTCGGG C^[CT]CG[GA]G 151 CTCGGG BsrBI 1060 GAGCGG CCGCTC(-3/-3) 1288 GAGCGG CelII 261 GCTGAGC GC^T[ACGT]AGC 648 GCTCAGC Hin1I 220 GACGTC G[GA]^CG[CT]C 410 GGCGCC MaeII 221 ACGT A^CGT 593 ACGT MaeIII 964 GTCAC ^GT[ACGT]AC 1000 GTGAC MwoI 418 GCCCAGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1065 GCGGCCCCGGC NaeI 320 GCCGGC GCC^GGC 407 GCCGGC NgoMIV 320 GCCGGC G^CCGGC 407 GCCGGC PpuMI 101 GGGTCCC [GA]G^G[AT]CC[CT] 1265 AGGACCC SfaNI 645 GATGC GCATC(5/9) 1344 GATGC SgrAI 406 CGCCGGCG C[GA]^CCGG[CT]G 1366 CGCCGGTG SmlI 82 CTCAAG C^T[CT][GA]AG 1329 CTTGAG TaqI 557 TCGA T^CGA 1466 TCGA Tsp45I 964 GTCAC ^GT[GC]AC 1000 GTGAC TthHB8I 557 TCGA T^CGA 1466 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AluI AlwNI AvaII BalI BanI BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HpaII HphI ItaI MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI NciI NdeII NlaIII NlaIV NspI PstI Sau3AI Sau96I ScrFI SfcI StuI TseI TspRI XcmI XhoII