(Circular) (Six-base) MAPSORT of: pFN21AE1959.seq Check: 2650 from: 1 to: 6253 pFN21AE1959 6253 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2229 4063 278 Size: 53 83 186 936 693 1834 2468 Fragments arranged by size: 2468 1834 936 693 186 83 53 AccI GT'mk_AC Cuts at: 1937 3471 3596 1937 Size: 1534 125 4594 Fragments arranged by size: 4594 1534 125 Acc65I G'GTAC_C Cuts at: 3574 3574 Size: 6253 AclI AA'CG_TT Cuts at: 1563 3552 4380 4753 1563 Size: 1989 828 373 3063 Fragments arranged by size: 3063 1989 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2362 4316 5700 5968 1632 Size: 730 1954 1384 268 1917 Fragments arranged by size: 1954 1917 1384 730 268 AfeI AGC'GCT Cuts at: 2216 2216 Size: 6253 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6056 AflIII A'CryG_T Cuts at: 1203 3520 5152 6129 1203 Size: 2317 1632 977 1327 Fragments arranged by size: 2317 1632 1327 977 AgeI A'CCGG_T Cuts at: 2395 5084 2395 Size: 2689 3564 AhdI GACnn_n'nnGTC Cuts at: 4982 4982 Size: 6253 AleI CACnn'nnGTG Cuts at: 1166 2255 1166 Size: 1089 5164 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6253 AlwNI CAG_nnn'CTG Cuts at: 2296 2390 2539 2552 3439 3996 5568 2296 Size: 94 149 13 887 557 1572 2981 Fragments arranged by size: 2981 1572 887 557 149 94 13 ApaLI G'TGCA_C Cuts at: 4310 5466 4310 Size: 1156 5097 ApoI r'AATT_y Cuts at: 1493 3562 3788 3940 6049 1493 Size: 2069 226 152 2109 1697 Fragments arranged by size: 2109 2069 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4807 160 Size: 872 3775 1606 Fragments arranged by size: 3775 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6253 AvaI C'yCGr_G Cuts at: 1870 1943 2103 2156 3578 1870 Size: 73 160 53 1422 4545 Fragments arranged by size: 4545 1422 160 73 53 BamHI G'GATC_C Cuts at: 3583 3950 3583 Size: 367 5886 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2415 2606 2858 3574 5029 Size: 325 217 627 628 191 252 716 1455 Cuts at: 5029 618 Size: 1842 Fragments arranged by size: 1842 1455 716 628 627 325 252 217 191 BanII G_rGCy'C Cuts at: 1391 3572 1391 Size: 2181 4072 BbeI G_GCGC'C Cuts at: 2419 2419 Size: 6253 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3217 928 Size: 971 1318 3964 Fragments arranged by size: 3964 1318 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3299 3333 4444 4478 1903 Size: 34 1362 34 1111 34 3678 Fragments arranged by size: 3678 1362 1111 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4145 5355 4145 Size: 1210 5043 BclI T'GATC_A Cuts at: 1548 2520 1548 Size: 972 5281 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4864 136 Size: 107 122 71 631 605 3192 1525 Fragments arranged by size: 3192 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3405 6248 3405 Size: 2843 3410 BlpI GC'TnA_GC Cuts at: 2267 2654 3670 2267 Size: 387 1016 4850 Fragments arranged by size: 4850 1016 387 Bme1580I G_kGCm'C Cuts at: 1790 2559 2863 4314 5470 1790 Size: 769 304 1451 1156 2573 Fragments arranged by size: 2573 1451 1156 769 304 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6253 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2011 3984 4942 Size: 416 53 83 399 24 594 1973 958 Cuts at: 4942 442 Size: 1753 Fragments arranged by size: 1973 1753 958 594 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6253 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2710 3292 2710 Size: 582 5671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2507 3007 3143 3461 4913 1239 Size: 1268 500 136 318 1452 2579 Fragments arranged by size: 2579 1452 1268 500 318 136 Bpu10I CC'TnA_GC Cuts at: 2511 3291 2511 Size: 780 5473 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2072 3355 4378 5243 5541 2072 Size: 1283 1023 865 298 2784 Fragments arranged by size: 2784 1283 1023 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3119 4916 882 Size: 617 1620 1797 2219 Fragments arranged by size: 2219 1797 1620 617 BsaAI yAC'GTr Cuts at: 493 1204 4044 493 Size: 711 2840 2702 Fragments arranged by size: 2840 2702 711 BsaBI GATnn'nnATC Cuts at: 6247 6247 Size: 6253 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2226 2416 4060 4442 Size: 53 83 186 936 693 190 1644 382 Cuts at: 4442 5077 275 Size: 635 1451 Fragments arranged by size: 1644 1451 936 693 635 382 190 186 83 53 BsaWI w'CCGG_w Cuts at: 2395 4686 5084 5358 5505 5916 2395 Size: 2291 398 274 147 411 2732 Fragments arranged by size: 2732 2291 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2429 2459 1178 Size: 30 165 30 1026 30 4972 Fragments arranged by size: 4972 1026 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2504 2686 3130 1186 Size: 1318 182 444 4309 Fragments arranged by size: 4309 1318 444 182 BseYI C'CCAG_C Cuts at: 2461 3093 5456 5952 2461 Size: 632 2363 496 2762 Fragments arranged by size: 2762 2363 632 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5680 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3703 4464 5492 1540 Size: 275 186 1702 761 1028 2301 Fragments arranged by size: 2301 1702 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2088 2817 4314 4399 5470 2088 Size: 729 1497 85 1071 2871 Fragments arranged by size: 2871 1497 1071 729 85 BsmI GAATG_Cn' Cuts at: 2434 3764 3857 2434 Size: 1330 93 4830 Fragments arranged by size: 4830 1330 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2559 2817 2863 3572 4314 4399 Size: 399 298 471 258 46 709 742 85 Cuts at: 4399 5470 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 709 471 399 298 258 85 46 BspEI T'CCGG_A Cuts at: 5916 5916 Size: 6253 BspHI T'CATG_A Cuts at: 2674 4142 5872 2674 Size: 1468 1730 3055 Fragments arranged by size: 3055 1730 1468 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2517 2745 3232 3608 844 Size: 1673 228 487 376 3489 Fragments arranged by size: 3489 1673 487 376 228 BsrBI CCG'CTC Cuts at: 1394 1823 3067 3295 4140 1394 Size: 429 1244 228 845 3507 Fragments arranged by size: 3507 1244 845 429 228 BsrDI GCAATG_nn' Cuts at: 65 1248 4748 4922 65 Size: 1183 3500 174 1396 Fragments arranged by size: 3500 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2325 2395 2412 3372 4897 5084 1700 Size: 110 515 70 17 960 1525 187 2869 Fragments arranged by size: 2869 1525 960 515 187 110 70 17 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6253 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6253 BssSI C'ACGA_G Cuts at: 2660 4313 5325 2660 Size: 1653 1012 3588 Fragments arranged by size: 3588 1653 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6253 BstBI TT'CG_AA Cuts at: 5057 5057 Size: 6253 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6253 BstXI CCAn_nnnn'nTGG Cuts at: 2139 2145 3264 2139 Size: 6 1119 5128 Fragments arranged by size: 5128 1119 6 BstYI r'GATC_y Cuts at: 1972 2235 3030 3405 3583 3950 4335 4352 5804 Size: 263 795 375 178 367 385 17 1452 Cuts at: 5804 5890 5902 6248 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 795 385 375 367 346 263 178 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2138 513 Size: 552 939 134 4628 Fragments arranged by size: 4628 939 552 134 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2434 2658 487 Size: 683 56 1208 224 4082 Fragments arranged by size: 4082 1208 683 224 56 BtsI GCAGTG_nn' Cuts at: 3315 3769 4563 4583 3315 Size: 454 794 20 4985 Fragments arranged by size: 4985 794 454 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6253 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6218 DraI TTT'AAA Cuts at: 3557 3557 Size: 6253 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5260 809 Size: 507 3944 1802 Fragments arranged by size: 3944 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2362 2592 Size: 54 1425 50 275 18 204 328 230 Cuts at: 2592 3393 3420 3700 4589 6218 8 Size: 801 27 280 889 1629 43 Fragments arranged by size: 1629 1425 889 801 328 280 275 230 204 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3700 1537 Size: 275 1888 4090 Fragments arranged by size: 4090 1888 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3419 4183 1353 Size: 2066 764 3423 Fragments arranged by size: 3423 2066 764 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4840 5214 5360 387 Size: 1064 138 3251 374 146 1280 Fragments arranged by size: 3251 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2362 2507 3007 3143 3461 4316 4913 Size: 393 730 145 500 136 318 855 597 Cuts at: 4913 5700 5968 1239 Size: 787 268 1524 Fragments arranged by size: 1524 855 787 730 597 500 393 318 268 145 136 EcoO109I rG'GnC_Cy Cuts at: 2107 2293 2515 2549 2885 3271 2107 Size: 186 222 34 336 386 5089 Fragments arranged by size: 5089 386 336 222 186 34 EcoRI G'AATT_C Cuts at: 3562 3940 3562 Size: 378 5875 FseI GG_CCGG'CC Cuts at: 2329 2329 Size: 6253 FspI TGC'GCA Cuts at: 4759 4759 Size: 6253 HaeII r_GCGC'y Cuts at: 1678 2218 2419 3373 5400 1678 Size: 540 201 954 2027 2531 Fragments arranged by size: 2531 2027 954 540 201 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4894 4926 4968 5000 1373 Size: 32 135 32 3322 32 42 32 2626 Fragments arranged by size: 3322 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2929 3247 3472 3597 3843 669 Size: 988 281 991 318 225 125 246 3079 Fragments arranged by size: 3079 991 988 318 281 246 225 125 HindIII A'AGCT_T Cuts at: 748 748 Size: 6253 HpaI GTT'AAC Cuts at: 3843 3843 Size: 6253 KasI G'GCGC_C Cuts at: 2415 2415 Size: 6253 KpnI G_GTAC'C Cuts at: 3578 3578 Size: 6253 MfeI C'AATT_G Cuts at: 3852 3852 Size: 6253 MluI A'CGCG_T Cuts at: 6129 6129 Size: 6253 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3157 5367 5551 1305 Size: 1852 2210 184 2007 Fragments arranged by size: 2210 2007 1852 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2364 2594 3395 3422 10 Size: 54 1425 343 532 230 801 27 2841 Fragments arranged by size: 2841 1425 801 532 343 230 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 2255 4211 4570 4729 518 Size: 648 1089 1956 359 159 2042 Fragments arranged by size: 2042 1956 1089 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2898 3097 3513 3669 4346 5494 5739 1606 Size: 1292 199 416 156 677 1148 245 2120 Fragments arranged by size: 2120 1292 1148 677 416 245 199 156 NaeI GCC'GGC Cuts at: 2327 2414 2327 Size: 87 6166 NarI GG'CG_CC Cuts at: 2416 2416 Size: 6253 NcoI C'CATG_G Cuts at: 513 1065 2004 2138 513 Size: 552 939 134 4628 Fragments arranged by size: 4628 939 552 134 NdeI CA'TA_TG Cuts at: 387 387 Size: 6253 NgoMIV G'CCGG_C Cuts at: 2325 2412 2325 Size: 87 6166 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6253 NotI GC'GGCC_GC Cuts at: 3700 3700 Size: 6253 NspI r_CATG'y Cuts at: 1132 1229 2165 2498 3524 3611 5156 1132 Size: 97 936 333 1026 87 1545 2229 Fragments arranged by size: 2229 1545 1026 936 333 97 87 PasI CC'CwG_GG Cuts at: 1348 2173 1348 Size: 825 5428 PciI A'CATG_T Cuts at: 3520 5152 3520 Size: 1632 4621 PflMI CCAn_nnn'nTGG Cuts at: 1108 2985 1108 Size: 1877 4376 PfoI T'CCnGG_A Cuts at: 2699 2808 2844 2984 3120 2699 Size: 109 36 140 136 5832 Fragments arranged by size: 5832 140 136 109 36 PmeI GTTT'AAAC Cuts at: 3557 3557 Size: 6253 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4322 4354 5835 5867 4322 Size: 32 1481 32 4708 Fragments arranged by size: 4708 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2107 3271 2107 Size: 1164 5089 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6253 PsiI TTA'TAA Cuts at: 3823 3823 Size: 6253 PstI C_TGCA'G Cuts at: 830 2235 2757 2799 3605 830 Size: 1405 522 42 806 3478 Fragments arranged by size: 3478 1405 806 522 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6253 PvuII CAG'CTG Cuts at: 3097 3097 Size: 6253 SalI G'TCGA_C Cuts at: 1936 3470 3595 1936 Size: 1534 125 4594 Fragments arranged by size: 4594 1534 125 SanDI GG'GwC_CC Cuts at: 2107 2107 Size: 6253 SbfI CC_TGCA'GG Cuts at: 3605 3605 Size: 6253 ScaI AGT'ACT Cuts at: 4501 6213 4501 Size: 1712 4541 SfcI C'TryA_G Cuts at: 826 1045 2231 2753 2795 3431 3601 4736 5417 Size: 219 1186 522 42 636 170 1135 681 Cuts at: 5417 5608 826 Size: 191 1471 Fragments arranged by size: 1471 1186 1135 681 636 522 219 191 170 42 SfoI GGC'GCC Cuts at: 2417 2417 Size: 6253 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6253 SgrAI Cr'CCGG_yG Cuts at: 1700 2412 3372 1700 Size: 712 960 4581 Fragments arranged by size: 4581 960 712 SmaI CCC'GGG Cuts at: 1872 3580 1872 Size: 1708 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2087 3334 4357 5258 5520 820 Size: 197 926 144 1247 1023 901 262 1553 Fragments arranged by size: 1553 1247 1023 926 901 262 197 144 SnaBI TAC'GTA Cuts at: 493 4044 493 Size: 3551 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6253 SphI G_CATG'C Cuts at: 1132 3611 1132 Size: 2479 3774 SspI AAT'ATT Cuts at: 5 52 4177 5 Size: 47 4125 2081 Fragments arranged by size: 4125 2081 47 StuI AGG'CCT Cuts at: 1517 2101 2118 2768 3113 1517 Size: 584 17 650 345 4657 Fragments arranged by size: 4657 650 584 345 17 StyI C'CwwG_G Cuts at: 513 1065 2004 2138 3692 513 Size: 552 939 134 1554 3074 Fragments arranged by size: 3074 1554 939 552 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2071 4280 4297 4450 4635 5080 325 Size: 1255 491 2209 17 153 185 445 1498 Fragments arranged by size: 2209 1498 1255 491 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4499 6211 96 Size: 274 80 33 51 1443 2522 1712 138 Fragments arranged by size: 2522 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6213 6245 6213 Size: 32 6221 Tth111I GACn'n_nGTC Cuts at: 1316 2966 1316 Size: 1650 4603 XbaI T'CTAG_A Cuts at: 3589 3589 Size: 6253 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2068 2136 2257 2068 Size: 68 121 6064 Fragments arranged by size: 6064 121 68 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6253 XmaI C'CCGG_G Cuts at: 1870 3578 1870 Size: 1708 4545 XmnI GAAnn'nnTTC Cuts at: 3197 4382 3197 Size: 1185 5068 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2227 4061 276 Size: 53 83 186 936 693 1834 2468 Fragments arranged by size: 2468 1834 936 693 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI