(Linear) (Six-base) MAPSORT of: KIEE1959.seq Check: 7833 from: 1 to: 1548 KIEE1959.orf With 182 enzymes: SgfI * July 3, 2012 17:05 .. AatII G_ACGT'C Cuts at: 0 224 1548 Size: 224 1324 AccI GT'mk_AC Cuts at: 0 1466 1548 Size: 1466 82 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 357 1548 Size: 357 1191 AfeI AGC'GCT Cuts at: 0 211 1548 Size: 211 1337 AflIII A'CryG_T Cuts at: 0 1515 1548 Size: 1515 33 AgeI A'CCGG_T Cuts at: 0 390 1548 Size: 390 1158 AleI CACnn'nnGTG Cuts at: 0 250 1548 Size: 250 1298 AlwNI CAG_nnn'CTG Cuts at: 0 291 385 534 547 1434 1548 Size: 291 94 149 13 887 114 Fragments arranged by size: 887 291 149 114 94 13 AvaI C'yCGr_G Cuts at: 0 98 151 1548 Size: 98 53 1397 Fragments arranged by size: 1397 98 53 BanI G'GyrC_C Cuts at: 0 410 601 853 1548 Size: 410 191 252 695 Fragments arranged by size: 695 410 252 191 BbeI G_GCGC'C Cuts at: 0 414 1548 Size: 414 1134 BbsI GAAGACnn'nnnn_ Cuts at: 0 1212 1548 Size: 1212 336 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1294 1328 1548 Size: 1294 34 220 Fragments arranged by size: 1294 220 34 BclI T'GATC_A Cuts at: 0 515 1548 Size: 515 1033 BglII A'GATC_T Cuts at: 0 1400 1548 Size: 1400 148 BlpI GC'TnA_GC Cuts at: 0 262 649 1548 Size: 262 387 899 Fragments arranged by size: 899 387 262 Bme1580I G_kGCm'C Cuts at: 0 554 858 1548 Size: 554 304 690 Fragments arranged by size: 690 554 304 BmrI ACTGGGnnnn_n' Cuts at: 0 6 1548 Size: 6 1542 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 705 1287 1548 Size: 705 582 261 Fragments arranged by size: 705 582 261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 1002 1138 1456 1548 Size: 502 500 136 318 92 Fragments arranged by size: 502 500 318 136 92 Bpu10I CC'TnA_GC Cuts at: 0 506 1286 1548 Size: 506 780 262 Fragments arranged by size: 780 506 262 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 1350 1548 Size: 67 1283 198 Fragments arranged by size: 1283 198 67 BsaI GGTCTCn'nnnn_ Cuts at: 0 1114 1548 Size: 1114 434 BsaHI Gr'CG_yC Cuts at: 0 221 411 1548 Size: 221 190 1137 Fragments arranged by size: 1137 221 190 BsaWI w'CCGG_w Cuts at: 0 390 1548 Size: 390 1158 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 424 454 1548 Size: 424 30 1094 Fragments arranged by size: 1094 424 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 499 681 1125 1548 Size: 499 182 444 423 Fragments arranged by size: 499 444 423 182 BseYI C'CCAG_C Cuts at: 0 456 1088 1548 Size: 456 632 460 Fragments arranged by size: 632 460 456 BsiHKAI G_wGCw'C Cuts at: 0 83 812 1548 Size: 83 729 736 Fragments arranged by size: 736 729 83 BsmI GAATG_Cn' Cuts at: 0 429 1548 Size: 429 1119 Bsp1286I G_dGCh'C Cuts at: 0 83 554 812 858 1548 Size: 83 471 258 46 690 Fragments arranged by size: 690 471 258 83 46 BspHI T'CATG_A Cuts at: 0 669 1548 Size: 669 879 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 512 740 1227 1548 Size: 512 228 487 321 Fragments arranged by size: 512 487 321 228 BsrBI CCG'CTC Cuts at: 0 1062 1290 1548 Size: 1062 228 258 Fragments arranged by size: 1062 258 228 BsrFI r'CCGG_y Cuts at: 0 320 390 407 1367 1548 Size: 320 70 17 960 181 Fragments arranged by size: 960 320 181 70 17 BssSI C'ACGA_G Cuts at: 0 655 1548 Size: 655 893 BstXI CCAn_nnnn'nTGG Cuts at: 0 134 140 1259 1548 Size: 134 6 1119 289 Fragments arranged by size: 1119 289 134 6 BstYI r'GATC_y Cuts at: 0 230 1025 1400 1548 Size: 230 795 375 148 Fragments arranged by size: 795 375 230 148 BtgI C'CryG_G Cuts at: 0 133 1548 Size: 133 1415 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 429 653 1548 Size: 429 224 895 Fragments arranged by size: 895 429 224 BtsI GCAGTG_nn' Cuts at: 0 1310 1548 Size: 1310 238 EaeI y'GGCC_r Cuts at: 0 29 357 587 1388 1415 1548 Size: 29 328 230 801 27 133 Fragments arranged by size: 801 328 230 133 29 27 EarI CTCTTCn'nnn_ Cuts at: 0 1414 1548 Size: 1414 134 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 357 502 1002 1138 1456 1548 Size: 357 145 500 136 318 92 Fragments arranged by size: 500 357 318 145 136 92 EcoO109I rG'GnC_Cy Cuts at: 0 102 288 510 544 880 1266 1548 Size: 102 186 222 34 336 386 282 Fragments arranged by size: 386 336 282 222 186 102 34 FseI GG_CCGG'CC Cuts at: 0 324 1548 Size: 324 1224 HaeII r_GCGC'y Cuts at: 0 213 414 1368 1548 Size: 213 201 954 180 Fragments arranged by size: 954 213 201 180 HincII GTy'rAC Cuts at: 0 924 1242 1467 1548 Size: 924 318 225 81 Fragments arranged by size: 924 318 225 81 KasI G'GCGC_C Cuts at: 0 410 1548 Size: 410 1138 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1152 1548 Size: 1152 396 MscI TGG'CCA Cuts at: 0 359 589 1390 1417 1548 Size: 359 230 801 27 131 Fragments arranged by size: 801 359 230 131 27 MslI CAynn'nnrTG Cuts at: 0 250 1548 Size: 250 1298 MspA1I CmG'CkG Cuts at: 0 893 1092 1508 1548 Size: 893 199 416 40 Fragments arranged by size: 893 416 199 40 NaeI GCC'GGC Cuts at: 0 322 409 1548 Size: 322 87 1139 Fragments arranged by size: 1139 322 87 NarI GG'CG_CC Cuts at: 0 411 1548 Size: 411 1137 NcoI C'CATG_G Cuts at: 0 133 1548 Size: 133 1415 NgoMIV G'CCGG_C Cuts at: 0 320 407 1548 Size: 320 87 1141 Fragments arranged by size: 1141 320 87 NspI r_CATG'y Cuts at: 0 160 493 1519 1548 Size: 160 333 1026 29 Fragments arranged by size: 1026 333 160 29 PasI CC'CwG_GG Cuts at: 0 168 1548 Size: 168 1380 PciI A'CATG_T Cuts at: 0 1515 1548 Size: 1515 33 PflMI CCAn_nnn'nTGG Cuts at: 0 980 1548 Size: 980 568 PfoI T'CCnGG_A Cuts at: 0 694 803 839 979 1115 1548 Size: 694 109 36 140 136 433 Fragments arranged by size: 694 433 140 136 109 36 PpuMI rG'GwC_Cy Cuts at: 0 102 1266 1548 Size: 102 1164 282 Fragments arranged by size: 1164 282 102 PstI C_TGCA'G Cuts at: 0 230 752 794 1548 Size: 230 522 42 754 Fragments arranged by size: 754 522 230 42 PvuII CAG'CTG Cuts at: 0 1092 1548 Size: 1092 456 SalI G'TCGA_C Cuts at: 0 1465 1548 Size: 1465 83 SanDI GG'GwC_CC Cuts at: 0 102 1548 Size: 102 1446 SfcI C'TryA_G Cuts at: 0 226 748 790 1426 1548 Size: 226 522 42 636 122 Fragments arranged by size: 636 522 226 122 42 SfoI GGC'GCC Cuts at: 0 412 1548 Size: 412 1136 SgrAI Cr'CCGG_yG Cuts at: 0 407 1367 1548 Size: 407 960 181 Fragments arranged by size: 960 407 181 SmlI C'TyrA_G Cuts at: 0 82 1329 1548 Size: 82 1247 219 Fragments arranged by size: 1247 219 82 StuI AGG'CCT Cuts at: 0 96 113 763 1108 1548 Size: 96 17 650 345 440 Fragments arranged by size: 650 440 345 96 17 StyI C'CwwG_G Cuts at: 0 133 1548 Size: 133 1415 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 66 1548 Size: 66 1482 Tth111I GACn'n_nGTC Cuts at: 0 961 1548 Size: 961 587 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 63 131 252 1548 Size: 63 68 121 1296 Fragments arranged by size: 1296 121 68 63 XmnI GAAnn'nnTTC Cuts at: 0 1192 1548 Size: 1192 356 ZraI GAC'GTC Cuts at: 0 222 1548 Size: 222 1326 Enzymes that do cut: AatII AccI AcuI AfeI AflIII AgeI AleI AlwNI AvaI BanI BbeI BbsI BcgI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI EaeI EarI Eco57MI EcoO109I FseI HaeII HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PstI PvuII SalI SanDI SfcI SfoI SgrAI SmlI StuI StyI TaqII Tth111I XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII BbvCI BciVI BfrBI BglI BmgBI BmtI BsaAI BsaBI BsgI BsiEI BsiWI BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XhoI XmaI