Restriction map for
pFN21AE1187
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccI AccIII AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbrPI BbvCI BciVI BclI BfrI
BlnI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DraII
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRV FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 1393 GACGTC GACGT^C
AclI 504 AACGTT AA^CGTT
AocI 2059 CCTCAGG CC^T[ACGT]AGG
Asp700I 1287 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2126 TGGCCA TGG^CCA
BamHI 2016 GGATCC G^GATCC
BcgI 1572 GCACAGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1134 CCCGG CC^[GC]GG
BglI 1937 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1794 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1794 GCTCAGC GC^T[ACGT]AGC
BseRI 1789 GAGGAG GAGGAG(10/8)
BsgI 603 CTGCAC GTGCAG(16/14)
BsmBI 1114 GAGACG CGTCTC(1/5)
Bsp1407I 245 TGTACA T^GTACA
BspLU11I 1690 ACATGT A^CATGT
BsrFI 2095 GCCGGT [GA]^CCGG[CT]
BsrGI 245 TGTACA T^GTACA
Bsu36I 2059 CCTCAGG CC^T[ACGT]AGG
CelII 1794 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2095 GCCGGT [GA]^CCGG[CT]
DraIII 1903 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1161 GACACTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2126 TGGCCA [CT]^GGCC[GA]
Eco81I 2059 CCTCAGG CC^T[ACGT]AGG
EcoT14I 60 CCAAGG C^C[AT][AT]GG
EcoT22I 789 ATGCAT ATGCA^T
MluI 1184 ACGCGT A^CGCGT
MluNI 2126 TGGCCA TGG^CCA
MscI 2126 TGGCCA TGG^CCA
NciI 1134 CCCGG CC^[GC]GG
NsiI 789 ATGCAT ATGCA^T
PaeR7I 1822 CTCGAG C^TCGAG
Psp1406I 504 AACGTT AA^CGTT
SmlI 1822 CTCGAG C^T[CT][GA]AG
SspBI 245 TGTACA T^GTACA
StyI 60 CCAAGG C^C[AT][AT]GG
XbaI 258 TCTAGA T^CTAGA
XhoI 1822 CTCGAG C^TCGAG
XmnI 1287 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 1185 CGCG CG^CG
1663 CGCG
AcyI 871 GACGCC G[GA]^CG[CT]C
1393 GACGTC
AvaI 805 CCCGAG C^[CT]CG[GA]G
1822 CTCGAG
BsaHI 871 GACGCC G[GA]^CG[CT]C
1393 GACGTC
BsaI 1810 GAGACC GGTCTC(1/5)
1244 GGTCTC
BsoBI 805 CCCGAG C^[CT]CG[GA]G
1822 CTCGAG
BspMI 559 GCAGGT ACCTGC(4/8)
1303 GCAGGT
BsrBI 1719 CCGCTC CCGCTC(-3/-3)
1594 GAGCGG
BstEII 1642 GGTGACC G^GT[ACGT]ACC
2170 GGTGACC
BstPI 1642 GGTGACC G^GT[ACGT]ACC
2170 GGTGACC
BstUI 1185 CGCG CG^CG
1663 CGCG
BstXI 821 CCAACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1977 CCAACCTCATGG
CfoI 112 GCGC GCG^C
1664 GCGC
DsaI 749 CCACGG C^C[GA][CT]GG
2163 CCACGG
EcoO65I 1642 GGTGACC G^GT[ACGT]ACC
2170 GGTGACC
EcoRI 165 GAATTC G^AATTC
1577 GAATTC
HhaI 112 GCGC GCG^C
1664 GCGC
Hin1I 871 GACGCC G[GA]^CG[CT]C
1393 GACGTC
HinP1I 112 GCGC G^CGC
1664 GCGC
MvnI 1185 CGCG CG^CG
1663 CGCG
NheI 1042 GCTAGC G^CTAGC
2253 GCTAGC
PflMI 1902 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2092 CCAGCCGGTGG
SacI 1792 GAGCTC GAGCT^C
1836 GAGCTC
SphI 380 GCATGC GCATG^C
525 GCATGC
Van91I 1902 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2092 CCAGCCGGTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
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AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII