(Circular) (Six-base) MAPSORT of: pFN21AE1187.seq Check: 8638 from: 1 to: 7000 pFN21AE1187 7000 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2555 2555 Size: 7000 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3402 4810 278 Size: 53 83 186 936 1866 1408 2468 Fragments arranged by size: 2468 1866 1408 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4343 1937 Size: 2406 4594 Acc65I G'GTAC_C Cuts at: 4321 4321 Size: 7000 AclI AA'CG_TT Cuts at: 1563 2510 5127 5500 1563 Size: 947 2617 373 3063 Fragments arranged by size: 3063 2617 947 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2580 2951 2969 3084 3153 3155 3460 5063 Size: 948 371 18 115 69 2 305 1603 Cuts at: 5063 6447 6715 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1603 1384 948 371 305 268 115 69 18 2 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6803 AflIII A'CryG_T Cuts at: 1203 3189 3695 3864 5899 6876 1203 Size: 1986 506 169 2035 977 1327 Fragments arranged by size: 2035 1986 1327 977 506 169 AgeI A'CCGG_T Cuts at: 5831 5831 Size: 7000 AhdI GACnn_n'nnGTC Cuts at: 5729 5729 Size: 7000 AleI CACnn'nnGTG Cuts at: 1166 3442 3444 3869 4173 1166 Size: 2276 2 425 304 3993 Fragments arranged by size: 3993 2276 425 304 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2547 2633 2742 1184 Size: 1363 86 109 5442 Fragments arranged by size: 5442 1363 109 86 AlwNI CAG_nnn'CTG Cuts at: 2047 2239 2366 3392 4743 6315 2047 Size: 192 127 1026 1351 1572 2732 Fragments arranged by size: 2732 1572 1351 1026 192 127 ApaLI G'TGCA_C Cuts at: 5057 6213 5057 Size: 1156 5844 ApoI r'AATT_y Cuts at: 1493 2170 3100 3582 3919 3932 4309 4535 4687 Size: 677 930 482 337 13 377 226 152 Cuts at: 4687 6796 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 930 677 482 377 337 226 152 13 AseI AT'TA_AT Cuts at: 160 1032 5554 160 Size: 872 4522 1606 Fragments arranged by size: 4522 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 AvaI C'yCGr_G Cuts at: 1870 1943 2810 3827 4325 1870 Size: 73 867 1017 498 4545 Fragments arranged by size: 4545 1017 867 498 73 BamHI G'GATC_C Cuts at: 4021 4330 4697 4021 Size: 309 367 6324 Fragments arranged by size: 6324 367 309 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4321 5776 618 Size: 325 217 627 2534 1455 1842 Fragments arranged by size: 2534 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3782 3801 3845 4319 1391 Size: 2391 19 44 474 4072 Fragments arranged by size: 4072 2391 474 44 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3141 3159 3514 4274 928 Size: 971 1242 18 355 760 3654 Fragments arranged by size: 3654 1242 971 760 355 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3566 3600 5191 5225 1903 Size: 34 1629 34 1591 34 3678 Fragments arranged by size: 3678 1629 1591 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4892 6102 4892 Size: 1210 5790 BclI T'GATC_A Cuts at: 1548 1548 Size: 7000 BfrBI ATG'CAT Cuts at: 2796 2796 Size: 7000 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3948 5611 136 Size: 107 122 71 631 605 2276 1663 1525 Fragments arranged by size: 2276 1663 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2266 3366 3702 6995 2266 Size: 1100 336 3293 2271 Fragments arranged by size: 3293 2271 1100 336 BlpI GC'TnA_GC Cuts at: 3800 4417 3800 Size: 617 6383 Bme1580I G_kGCm'C Cuts at: 1790 5061 6217 1790 Size: 3271 1156 2573 Fragments arranged by size: 3271 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3867 1318 Size: 2549 4451 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2691 3687 3905 Size: 416 53 83 399 24 1274 996 218 Cuts at: 3905 4731 5689 442 Size: 826 958 1753 Fragments arranged by size: 1753 1274 996 958 826 416 399 218 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3051 4262 1056 Size: 1995 1211 3794 Fragments arranged by size: 3794 1995 1211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2024 2822 3812 4222 5660 1239 Size: 785 798 990 410 1438 2579 Fragments arranged by size: 2579 1438 990 798 785 410 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5125 5990 6288 5125 Size: 865 298 5837 Fragments arranged by size: 5837 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3255 3809 5663 882 Size: 617 1756 554 1854 2219 Fragments arranged by size: 2219 1854 1756 617 554 BsaAI yAC'GTr Cuts at: 493 1204 4791 493 Size: 711 3587 2702 Fragments arranged by size: 3587 2702 711 BsaBI GATnn'nnATC Cuts at: 6994 6994 Size: 7000 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2877 3399 4807 5189 Size: 53 83 186 936 1344 522 1408 382 Cuts at: 5189 5824 275 Size: 635 1451 Fragments arranged by size: 1451 1408 1344 936 635 522 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5433 5831 6105 6252 6663 5433 Size: 398 274 147 411 5770 Fragments arranged by size: 5770 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3674 3704 3839 3869 1178 Size: 30 165 30 2271 30 135 30 4309 Fragments arranged by size: 4309 2271 165 135 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3809 1186 Size: 2623 4377 BseYI C'CCAG_C Cuts at: 2838 4096 6203 6699 2838 Size: 1258 2107 496 3139 Fragments arranged by size: 3139 2107 1258 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2593 1163 Size: 573 857 5570 Fragments arranged by size: 5570 857 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4450 5211 6239 1540 Size: 275 186 2449 761 1028 2301 Fragments arranged by size: 2449 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2817 3495 3738 3801 3845 5061 5146 6217 2817 Size: 678 243 63 44 1216 85 1071 3600 Fragments arranged by size: 3600 1216 1071 678 243 85 63 44 BsmI GAATG_Cn' Cuts at: 2138 2798 3337 4511 4604 2138 Size: 660 539 1174 93 4534 Fragments arranged by size: 4534 1174 660 539 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3113 3113 Size: 7000 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2817 3495 3738 3782 3801 3845 4319 Size: 399 1027 678 243 44 19 44 474 Cuts at: 4319 5061 5146 6217 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1027 742 678 474 399 243 85 44 44 19 BspEI T'CCGG_A Cuts at: 6663 6663 Size: 7000 BspHI T'CATG_A Cuts at: 4889 6619 4889 Size: 1730 5270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2555 3299 4355 844 Size: 1711 744 1056 3489 Fragments arranged by size: 3489 1711 1056 744 BsrBI CCG'CTC Cuts at: 1394 1823 3601 3726 4887 1394 Size: 429 1778 125 1161 3507 Fragments arranged by size: 3507 1778 1161 429 125 BsrDI GCAATG_nn' Cuts at: 65 1248 5495 5669 65 Size: 1183 4247 174 1396 Fragments arranged by size: 4247 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4100 5644 5831 1700 Size: 110 2290 1544 187 2869 Fragments arranged by size: 2869 2290 1544 187 110 BsrGI T'GTAC_A Cuts at: 96 2250 96 Size: 2154 4846 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7000 BssSI C'ACGA_G Cuts at: 5060 6072 5060 Size: 1012 5988 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7000 BstBI TT'CG_AA Cuts at: 5804 5804 Size: 7000 BstEII G'GTnAC_C Cuts at: 1184 3647 4175 1184 Size: 2463 528 4009 Fragments arranged by size: 4009 2463 528 BstXI CCAn_nnnn'nTGG Cuts at: 2833 3989 2833 Size: 1156 5844 BstYI r'GATC_y Cuts at: 1972 2235 2266 3366 3702 4021 4330 4697 5082 Size: 263 31 1100 336 319 309 367 385 Cuts at: 5082 5099 6551 6637 6649 6995 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1100 385 367 346 336 319 309 263 86 31 17 12 Bsu36I CC'TnA_GG Cuts at: 4065 4065 Size: 7000 BtgI C'CryG_G Cuts at: 513 1065 2004 2754 4168 513 Size: 552 939 750 1414 3345 Fragments arranged by size: 3345 1414 939 750 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4277 487 Size: 683 56 3051 3210 Fragments arranged by size: 3210 3051 683 56 BtsI GCAGTG_nn' Cuts at: 3757 4112 4516 5310 5330 3757 Size: 355 404 794 20 5427 Fragments arranged by size: 5427 794 404 355 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7000 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6965 DraI TTT'AAA Cuts at: 2113 2355 3217 4304 2113 Size: 242 862 1087 4809 Fragments arranged by size: 4809 1087 862 242 DraIII CAC_nnn'GTG Cuts at: 3913 3913 Size: 7000 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3172 6007 809 Size: 507 1856 2835 1802 Fragments arranged by size: 2835 1856 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4131 4447 5336 Size: 54 1425 50 275 18 2301 316 889 Cuts at: 5336 6965 8 Size: 1629 43 Fragments arranged by size: 2301 1629 1425 889 316 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4447 1537 Size: 275 2635 4090 Fragments arranged by size: 4090 2635 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4930 1353 Size: 3577 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5587 5961 6107 387 Size: 1064 138 3998 374 146 1280 Fragments arranged by size: 3998 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3799 3843 3799 Size: 44 6956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2024 2580 2822 2951 2969 3084 3153 Size: 393 392 556 242 129 18 115 69 Cuts at: 3153 3155 3460 3812 4222 5063 5660 6447 6715 Size: 2 305 352 410 841 597 787 268 Cuts at: 6715 1239 Size: 1524 Fragments arranged by size: 1524 841 787 597 556 410 393 392 352 305 268 242 129 115 69 18 2 EcoRI G'AATT_C Cuts at: 2170 3582 4309 4687 2170 Size: 1412 727 378 4483 Fragments arranged by size: 4483 1412 727 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2090 2090 Size: 7000 FspI TGC'GCA Cuts at: 5506 5506 Size: 7000 HaeII r_GCGC'y Cuts at: 1678 6147 1678 Size: 4469 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2326 2358 2960 2992 3839 Size: 32 135 32 754 32 602 32 847 Cuts at: 3839 3871 3929 3961 3970 4002 4006 4038 4048 Size: 32 58 32 9 32 4 32 10 Cuts at: 4048 4080 5641 5673 5715 5747 1373 Size: 32 1561 32 42 32 2626 Fragments arranged by size: 2626 1561 847 754 602 135 58 42 32 32 32 32 32 32 32 32 32 32 32 10 9 4 HincII GTy'rAC Cuts at: 669 1657 1938 4344 4590 669 Size: 988 281 2406 246 3079 Fragments arranged by size: 3079 2406 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7000 HpaI GTT'AAC Cuts at: 4590 4590 Size: 7000 KpnI G_GTAC'C Cuts at: 4325 4325 Size: 7000 MfeI C'AATT_G Cuts at: 4599 4599 Size: 7000 MluI A'CGCG_T Cuts at: 3189 6876 3189 Size: 3687 3313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2493 2850 2994 3846 4006 6114 6298 1305 Size: 1188 357 144 852 160 2108 184 2007 Fragments arranged by size: 2108 2007 1188 852 357 184 160 144 MscI TGG'CCA Cuts at: 10 64 1489 1832 4133 10 Size: 54 1425 343 2301 2877 Fragments arranged by size: 2877 2301 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2124 2205 2529 3258 3342 3442 3444 Size: 648 958 81 324 729 84 100 2 Cuts at: 3444 3869 4133 4173 4958 5317 5476 518 Size: 425 264 40 785 359 159 2042 Fragments arranged by size: 2042 958 785 729 648 425 359 324 264 159 100 84 81 40 2 MspA1I CmG'CkG Cuts at: 1606 2028 2607 2690 3612 4416 5093 6241 6486 Size: 422 579 83 922 804 677 1148 245 Cuts at: 6486 1606 Size: 2120 Fragments arranged by size: 2120 1148 922 804 677 579 422 245 83 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5509 Fragments arranged by size: 5509 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7000 NheI G'CTAG_C Cuts at: 1052 3047 4258 1052 Size: 1995 1211 3794 Fragments arranged by size: 3794 1995 1211 NotI GC'GGCC_GC Cuts at: 4447 4447 Size: 7000 NsiI A_TGCA'T Cuts at: 2798 2798 Size: 7000 NspI r_CATG'y Cuts at: 1132 1229 2389 2534 2886 3665 3699 4358 5903 Size: 97 1160 145 352 779 34 659 1545 Cuts at: 5903 1132 Size: 2229 Fragments arranged by size: 2229 1545 1160 779 659 352 145 97 34 PasI CC'CwG_GG Cuts at: 1348 4183 1348 Size: 2835 4165 PciI A'CATG_T Cuts at: 3695 5899 3695 Size: 2204 4796 PflMI CCAn_nnn'nTGG Cuts at: 1108 3913 4103 1108 Size: 2805 190 4005 Fragments arranged by size: 4005 2805 190 PfoI T'CCnGG_A Cuts at: 2238 2799 2238 Size: 561 6439 PmeI GTTT'AAAC Cuts at: 4304 4304 Size: 7000 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5069 5101 6582 6614 5069 Size: 32 1481 32 5455 Fragments arranged by size: 5455 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7000 PsiI TTA'TAA Cuts at: 4570 4570 Size: 7000 PstI C_TGCA'G Cuts at: 830 2478 4119 4278 4352 830 Size: 1648 1641 159 74 3478 Fragments arranged by size: 3478 1648 1641 159 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7000 PvuII CAG'CTG Cuts at: 2028 2607 2690 3612 2028 Size: 579 83 922 5416 Fragments arranged by size: 5416 922 579 83 SacI G_AGCT'C Cuts at: 3801 3845 3801 Size: 44 6956 SalI G'TCGA_C Cuts at: 1936 4342 1936 Size: 2406 4594 SbfI CC_TGCA'GG Cuts at: 4352 4352 Size: 7000 ScaI AGT'ACT Cuts at: 5248 6960 5248 Size: 1712 5288 SexAI A'CCwGG_T Cuts at: 4296 4296 Size: 7000 SfcI C'TryA_G Cuts at: 826 1045 2367 2474 2742 4115 4274 4348 5483 Size: 219 1322 107 268 1373 159 74 1135 Cuts at: 5483 6164 6355 826 Size: 681 191 1471 Fragments arranged by size: 1471 1373 1322 1135 681 268 219 191 159 107 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7000 SmaI CCC'GGG Cuts at: 1872 4327 1872 Size: 2455 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3827 5104 6005 6267 820 Size: 197 926 1884 1277 901 262 1553 Fragments arranged by size: 1884 1553 1277 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4791 493 Size: 4298 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7000 SphI G_CATG'C Cuts at: 1132 2389 2534 4358 1132 Size: 1257 145 1824 3774 Fragments arranged by size: 3774 1824 1257 145 SspI AAT'ATT Cuts at: 5 52 4924 5 Size: 47 4872 2081 Fragments arranged by size: 4872 2081 47 StuI AGG'CCT Cuts at: 1517 2070 3130 4070 1517 Size: 553 1060 940 4447 Fragments arranged by size: 4447 1060 940 553 StyI C'CwwG_G Cuts at: 513 1065 2004 2065 4439 513 Size: 552 939 61 2374 3074 Fragments arranged by size: 3074 2374 939 552 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3237 3924 5027 5044 5197 5382 5827 Size: 1255 1657 687 1103 17 153 185 445 Cuts at: 5827 325 Size: 1498 Fragments arranged by size: 1657 1498 1255 1103 687 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2250 2371 2399 Size: 274 80 33 51 1443 273 121 28 Cuts at: 2399 5246 6958 96 Size: 2847 1712 138 Fragments arranged by size: 2847 1712 1443 274 273 138 121 80 51 33 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2807 2839 6960 6992 2807 Size: 32 4121 32 2815 Fragments arranged by size: 4121 2815 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7000 XbaI T'CTAG_A Cuts at: 2263 4336 2263 Size: 2073 4927 XhoI C'TCGA_G Cuts at: 1943 3827 1943 Size: 1884 5116 XmaI C'CCGG_G Cuts at: 1870 4325 1870 Size: 2455 4545 XmnI GAAnn'nnTTC Cuts at: 3296 5129 3296 Size: 1833 5167 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3400 4808 276 Size: 53 83 186 936 1866 1408 2468 Fragments arranged by size: 2468 1866 1408 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI