(Linear) (Six-base) MAPSORT of: KIEE1187.seq Check: 5906 from: 1 to: 2295 KIEE1187.orf With 182 enzymes: SgfI * July 3, 2012 16:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 550 2295 Size: 550 1745 AatII G_ACGT'C Cuts at: 0 1397 2295 Size: 1397 898 AclI AA'CG_TT Cuts at: 0 505 2295 Size: 505 1790 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 575 946 964 1079 1148 1150 1455 2295 Size: 575 371 18 115 69 2 305 840 Fragments arranged by size: 840 575 371 305 115 69 18 2 AflIII A'CryG_T Cuts at: 0 1184 1690 1859 2295 Size: 1184 506 169 436 Fragments arranged by size: 1184 506 436 169 AleI CACnn'nnGTG Cuts at: 0 1437 1439 1864 2168 2295 Size: 1437 2 425 304 127 Fragments arranged by size: 1437 425 304 127 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 542 628 737 2295 Size: 542 86 109 1558 Fragments arranged by size: 1558 542 109 86 AlwNI CAG_nnn'CTG Cuts at: 0 42 234 361 1387 2295 Size: 42 192 127 1026 908 Fragments arranged by size: 1026 908 192 127 42 ApoI r'AATT_y Cuts at: 0 165 1095 1577 1914 1927 2295 Size: 165 930 482 337 13 368 Fragments arranged by size: 930 482 368 337 165 13 AvaI C'yCGr_G Cuts at: 0 805 1822 2295 Size: 805 1017 473 Fragments arranged by size: 1017 805 473 BamHI G'GATC_C Cuts at: 0 2016 2295 Size: 2016 279 BanII G_rGCy'C Cuts at: 0 1777 1796 1840 2295 Size: 1777 19 44 455 Fragments arranged by size: 1777 455 44 19 BbsI GAAGACnn'nnnn_ Cuts at: 0 1136 1154 1509 2269 2295 Size: 1136 18 355 760 26 Fragments arranged by size: 1136 760 355 26 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1561 1595 2295 Size: 1561 34 700 Fragments arranged by size: 1561 700 34 BfrBI ATG'CAT Cuts at: 0 791 2295 Size: 791 1504 BglI GCCn_nnn'nGGC Cuts at: 0 1943 2295 Size: 1943 352 BglII A'GATC_T Cuts at: 0 261 1361 1697 2295 Size: 261 1100 336 598 Fragments arranged by size: 1100 598 336 261 BlpI GC'TnA_GC Cuts at: 0 1795 2295 Size: 1795 500 BmgBI CAC'GTC Cuts at: 0 1862 2295 Size: 1862 433 BmrI ACTGGGnnnn_n' Cuts at: 0 686 1682 1900 2295 Size: 686 996 218 395 Fragments arranged by size: 996 686 395 218 BmtI G_CTAG'C Cuts at: 0 1046 2257 2295 Size: 1046 1211 38 Fragments arranged by size: 1211 1046 38 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 19 817 1807 2217 2295 Size: 19 798 990 410 78 Fragments arranged by size: 990 798 410 78 19 BsaI GGTCTCn'nnnn_ Cuts at: 0 1250 1804 2295 Size: 1250 554 491 Fragments arranged by size: 1250 554 491 BsaHI Gr'CG_yC Cuts at: 0 872 1394 2295 Size: 872 522 901 Fragments arranged by size: 901 872 522 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1669 1699 1834 1864 2295 Size: 1669 30 135 30 431 Fragments arranged by size: 1669 431 135 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1804 2295 Size: 1804 491 BseYI C'CCAG_C Cuts at: 0 833 2091 2295 Size: 833 1258 204 Fragments arranged by size: 1258 833 204 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 588 2295 Size: 588 1707 BsiHKAI G_wGCw'C Cuts at: 0 812 1490 1733 1796 1840 2295 Size: 812 678 243 63 44 455 Fragments arranged by size: 812 678 455 243 63 44 BsmI GAATG_Cn' Cuts at: 0 133 793 1332 2295 Size: 133 660 539 963 Fragments arranged by size: 963 660 539 133 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1108 2295 Size: 1108 1187 Bsp1286I G_dGCh'C Cuts at: 0 812 1490 1733 1777 1796 1840 2295 Size: 812 678 243 44 19 44 455 Fragments arranged by size: 812 678 455 243 44 44 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 550 1294 2295 Size: 550 744 1001 Fragments arranged by size: 1001 744 550 BsrBI CCG'CTC Cuts at: 0 1596 1721 2295 Size: 1596 125 574 Fragments arranged by size: 1596 574 125 BsrFI r'CCGG_y Cuts at: 0 2095 2295 Size: 2095 200 BsrGI T'GTAC_A Cuts at: 0 245 2295 Size: 245 2050 BstEII G'GTnAC_C Cuts at: 0 1642 2170 2295 Size: 1642 528 125 Fragments arranged by size: 1642 528 125 BstXI CCAn_nnnn'nTGG Cuts at: 0 828 1984 2295 Size: 828 1156 311 Fragments arranged by size: 1156 828 311 BstYI r'GATC_y Cuts at: 0 230 261 1361 1697 2016 2295 Size: 230 31 1100 336 319 279 Fragments arranged by size: 1100 336 319 279 230 31 Bsu36I CC'TnA_GG Cuts at: 0 2060 2295 Size: 2060 235 BtgI C'CryG_G Cuts at: 0 749 2163 2295 Size: 749 1414 132 Fragments arranged by size: 1414 749 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2272 2295 Size: 2272 23 BtsI GCAGTG_nn' Cuts at: 0 1752 2107 2295 Size: 1752 355 188 Fragments arranged by size: 1752 355 188 DraI TTT'AAA Cuts at: 0 108 350 1212 2295 Size: 108 242 862 1083 Fragments arranged by size: 1083 862 242 108 DraIII CAC_nnn'GTG Cuts at: 0 1908 2295 Size: 1908 387 DrdI GACnn_nn'nnGTC Cuts at: 0 1167 2295 Size: 1167 1128 EaeI y'GGCC_r Cuts at: 0 2126 2295 Size: 2126 169 EcoICRI GAG'CTC Cuts at: 0 1794 1838 2295 Size: 1794 44 457 Fragments arranged by size: 1794 457 44 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 19 575 817 946 964 1079 1148 1150 Size: 19 556 242 129 18 115 69 2 Cuts at: 1150 1455 1807 2217 2295 Size: 305 352 410 78 Fragments arranged by size: 556 410 352 305 242 129 115 78 69 19 18 2 EcoRI G'AATT_C Cuts at: 0 165 1577 2295 Size: 165 1412 718 Fragments arranged by size: 1412 718 165 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 85 2295 Size: 85 2210 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 321 353 955 987 1834 1866 1924 1956 Size: 321 32 602 32 847 32 58 32 Cuts at: 1956 1965 1997 2001 2033 2043 2075 2295 Size: 9 32 4 32 10 32 220 Fragments arranged by size: 847 602 321 220 58 32 32 32 32 32 32 32 10 9 4 MluI A'CGCG_T Cuts at: 0 1184 2295 Size: 1184 1111 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 488 845 989 1841 2001 2295 Size: 488 357 144 852 160 294 Fragments arranged by size: 852 488 357 294 160 144 MscI TGG'CCA Cuts at: 0 2128 2295 Size: 2128 167 MslI CAynn'nnrTG Cuts at: 0 119 200 524 1253 1337 1437 1439 1864 Size: 119 81 324 729 84 100 2 425 Cuts at: 1864 2128 2168 2295 Size: 264 40 127 Fragments arranged by size: 729 425 324 264 127 119 100 84 81 40 2 MspA1I CmG'CkG Cuts at: 0 23 602 685 1607 2295 Size: 23 579 83 922 688 Fragments arranged by size: 922 688 579 83 23 NheI G'CTAG_C Cuts at: 0 1042 2253 2295 Size: 1042 1211 42 Fragments arranged by size: 1211 1042 42 NsiI A_TGCA'T Cuts at: 0 793 2295 Size: 793 1502 NspI r_CATG'y Cuts at: 0 384 529 881 1660 1694 2295 Size: 384 145 352 779 34 601 Fragments arranged by size: 779 601 384 352 145 34 PasI CC'CwG_GG Cuts at: 0 2178 2295 Size: 2178 117 PciI A'CATG_T Cuts at: 0 1690 2295 Size: 1690 605 PflMI CCAn_nnn'nTGG Cuts at: 0 1908 2098 2295 Size: 1908 190 197 Fragments arranged by size: 1908 197 190 PfoI T'CCnGG_A Cuts at: 0 233 794 2295 Size: 233 561 1501 Fragments arranged by size: 1501 561 233 PstI C_TGCA'G Cuts at: 0 473 2114 2273 2295 Size: 473 1641 159 22 Fragments arranged by size: 1641 473 159 22 PvuII CAG'CTG Cuts at: 0 23 602 685 1607 2295 Size: 23 579 83 922 688 Fragments arranged by size: 922 688 579 83 23 SacI G_AGCT'C Cuts at: 0 1796 1840 2295 Size: 1796 44 455 Fragments arranged by size: 1796 455 44 SfcI C'TryA_G Cuts at: 0 362 469 737 2110 2269 2295 Size: 362 107 268 1373 159 26 Fragments arranged by size: 1373 362 268 159 107 26 SmlI C'TyrA_G Cuts at: 0 1822 2295 Size: 1822 473 SphI G_CATG'C Cuts at: 0 384 529 2295 Size: 384 145 1766 Fragments arranged by size: 1766 384 145 StuI AGG'CCT Cuts at: 0 65 1125 2065 2295 Size: 65 1060 940 230 Fragments arranged by size: 1060 940 230 65 StyI C'CwwG_G Cuts at: 0 60 2295 Size: 60 2235 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1232 1919 2295 Size: 1232 687 376 Fragments arranged by size: 1232 687 376 TatI w'GTAC_w Cuts at: 0 245 366 394 2295 Size: 245 121 28 1901 Fragments arranged by size: 1901 245 121 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 802 834 2295 Size: 802 32 1461 Fragments arranged by size: 1461 802 32 XbaI T'CTAG_A Cuts at: 0 258 2295 Size: 258 2037 XhoI C'TCGA_G Cuts at: 0 1822 2295 Size: 1822 473 XmnI GAAnn'nnTTC Cuts at: 0 1291 2295 Size: 1291 1004 ZraI GAC'GTC Cuts at: 0 1395 2295 Size: 1395 900 Enzymes that do cut: AarI AatII AclI AcuI AflIII AleI AlfI AlwNI ApoI AvaI BamHI BanII BbsI BcgI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BpmI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EcoICRI Eco57MI EcoRI FalI Hin4I MluI MmeI MscI MslI MspA1I NheI NsiI NspI PasI PciI PflMI PfoI PstI PvuII SacI SfcI SmlI SphI StuI StyI TaqII TatI TstI XbaI XhoI XmnI ZraI Enzymes that do not cut: AccI Acc65I AfeI AflII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BciVI BclI Bme1580I BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrDI BssHII BssSI BstAPI BstBI BstZ17I ClaI CspCI EagI EarI EciI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI NaeI NarI NcoI NdeI NgoMIV NotI NruI PacI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XcmI XmaI