################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI ApoI AseI AsnI Asp718I AspEI AspI BbeI BbrPI BbsI BciVI BfrI BpuAI BsaAI BsaBI BsaHI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FseI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI ScaI SexAI SfoI SfuI SnaBI SpeI SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AflIII 1837 ACGCGT A^C[GA][CT]GT AscI 1487 GGCGCGCC GG^CGCGCC Asp700I 2118 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 511 CCTAGG C^CTAGG BamHI 960 GGATCC G^GATCC BanI 1118 GGCACC G^G[CT][GA]CC BbvCI 1113 GCTGAGG CCTCAGC(-5/-2) BcgI 336 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 989 TGATCA T^GATCA BglI 860 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 674 AGATCT A^GATCT BlnI 511 CCTAGG C^CTAGG BlpI 1408 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1408 GCTAAGC GC^T[ACGT]AGC BsaI 1908 GGTCTC GGTCTC(1/5) BsaWI 140 TCCGGT [AT]^CCGG[AT] BsmAI 1909 GTCTC GTCTC(1/5) CelII 1408 GCTAAGC GC^T[ACGT]AGC EarI 1282 GAAGAG CTCTTC(1/4) FbaI 989 TGATCA T^GATCA Ksp632I 1282 GAAGAG CTCTTC(1/4) MaeII 833 ACGT A^CGT MluI 1837 ACGCGT A^CGCGT NotI 1166 GCGGCCGC GC^GGCCGC SapI 1282 GAAGAGC GCTCTTC(1/4) SbfI 540 CCTGCAGG CCTGCA^GG SfiI 859 GGCCTTTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1395 CCCGGG CCC^GGG SmlI 1828 CTCAAG C^T[CT][GA]AG Sse8387I 540 CCTGCAGG CCTGCA^GG TaqI 1547 TCGA T^CGA TfiI 526 GAATC G^A[AT]TC Tsp45I 397 GTCAC ^GT[GC]AC TthHB8I 1547 TCGA T^CGA XcmI 684 CCACAGCAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1395 CCCGGG C^CCGGG XmaI 1395 CCCGGG C^CCGGG XmnI 2118 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### Alw44I 1297 GTGCAC G^TGCAC 1600 GTGCAC AocI 637 CCTGAGG CC^T[ACGT]AGG 700 CCTGAGG ApaLI 1297 GTGCAC G^TGCAC 1600 GTGCAC BstXI 648 CCAGAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2133 CCAGGACTGTGG Bsu36I 637 CCTGAGG CC^T[ACGT]AGG 700 CCTGAGG DsaI 950 CCACGG C^C[GA][CT]GG 1383 CCACGG Eco81I 637 CCTGAGG CC^T[ACGT]AGG 700 CCTGAGG EcoNI 633 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 710 CCTCATGGAGG EcoT14I 236 CCAAGG C^C[AT][AT]GG 511 CCTAGG MaeIII 397 GTCAC ^GT[ACGT]AC 565 GTTAC NspI 906 GCATGC [GA]CATG^[CT] 2044 GCATGC PflMI 487 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 995 CCACCCGCTGG SphI 906 GCATGC GCATG^C 2044 GCATGC StyI 236 CCAAGG C^C[AT][AT]GG 511 CCTAGG Van91I 487 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 995 CCACCCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI Aor51HI ApaI AspHI AvaI AvaII AviII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EagI EclXI Eco47III Eco52I EcoO109I EcoRII FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SgrAI StuI TseI TspRI XhoII