(Circular) (Six-base) MAPSORT of: pFN21AB6220.seq Check: 7756 from: 1 to: 6907 pFN21AB6220 6907 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2539 3207 2539 Size: 668 6239 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4717 278 Size: 53 83 186 936 3181 2468 Fragments arranged by size: 3181 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4250 1937 Size: 2313 4594 Acc65I G'GTAC_C Cuts at: 4228 4228 Size: 6907 AclI AA'CG_TT Cuts at: 1563 5034 5407 1563 Size: 3471 373 3063 Fragments arranged by size: 3471 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2145 3166 3805 4970 6354 6622 1632 Size: 513 1021 639 1165 1384 268 1917 Fragments arranged by size: 1917 1384 1165 1021 639 513 268 AfeI AGC'GCT Cuts at: 3359 3566 3662 3818 3359 Size: 207 96 156 6448 Fragments arranged by size: 6448 207 156 96 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6710 AflIII A'CryG_T Cuts at: 1203 3842 5806 6783 1203 Size: 2639 1964 977 1327 Fragments arranged by size: 2639 1964 1327 977 AgeI A'CCGG_T Cuts at: 5738 5738 Size: 6907 AhdI GACnn_n'nnGTC Cuts at: 5636 5636 Size: 6907 AleI CACnn'nnGTG Cuts at: 1166 3141 3885 4032 1166 Size: 1975 744 147 4041 Fragments arranged by size: 4041 1975 744 147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3131 1184 Size: 1947 4960 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3221 3253 3306 3338 3221 Size: 32 53 32 6790 Fragments arranged by size: 6790 53 32 32 AlwNI CAG_nnn'CTG Cuts at: 2123 2221 2254 2281 2498 4102 4650 6222 2123 Size: 98 33 27 217 1604 548 1572 2808 Fragments arranged by size: 2808 1604 1572 548 217 98 33 27 ApaI G_GGCC'C Cuts at: 2178 2505 2740 2987 3407 2178 Size: 327 235 247 420 5678 Fragments arranged by size: 5678 420 327 247 235 ApaLI G'TGCA_C Cuts at: 3302 3605 4964 6120 3302 Size: 303 1359 1156 4089 Fragments arranged by size: 4089 1359 1156 303 ApoI r'AATT_y Cuts at: 1493 4216 4442 4594 6703 1493 Size: 2723 226 152 2109 1697 Fragments arranged by size: 2723 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 3493 3493 Size: 6907 AseI AT'TA_AT Cuts at: 160 1032 5461 160 Size: 872 4429 1606 Fragments arranged by size: 4429 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6907 AvaI C'yCGr_G Cuts at: 1870 1943 2339 3400 3440 3803 3971 4232 1870 Size: 73 396 1061 40 363 168 261 4545 Fragments arranged by size: 4545 1061 396 363 261 168 73 40 AvrII C'CTAG_G Cuts at: 2516 2516 Size: 6907 BamHI G'GATC_C Cuts at: 2965 4237 4604 2965 Size: 1272 367 5268 Fragments arranged by size: 5268 1272 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3123 4228 5683 618 Size: 325 217 627 1336 1105 1455 1842 Fragments arranged by size: 1842 1455 1336 1105 627 325 217 BanII G_rGCy'C Cuts at: 1391 2027 2178 2451 2505 2740 2987 3407 4226 Size: 636 151 273 54 235 247 420 819 Cuts at: 4226 1391 Size: 4072 Fragments arranged by size: 4072 819 636 420 273 247 235 151 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5936 BbvCI CC'TCA_GC Cuts at: 3119 3119 Size: 6907 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2330 2364 5098 5132 1903 Size: 34 393 34 2734 34 3678 Fragments arranged by size: 3678 2734 393 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4799 6009 4799 Size: 1210 5697 BclI T'GATC_A Cuts at: 1548 2994 1548 Size: 1446 5461 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2871 5518 136 Size: 107 122 71 631 605 1199 2647 1525 Fragments arranged by size: 2647 1525 1199 631 605 122 107 71 BglII A'GATC_T Cuts at: 2679 6902 2679 Size: 4223 2684 BlpI GC'TnA_GC Cuts at: 3414 4324 3414 Size: 910 5997 Bme1580I G_kGCm'C Cuts at: 1790 2178 2505 2740 2987 3306 3407 3609 4968 Size: 388 327 235 247 319 101 202 1359 Cuts at: 4968 6124 1790 Size: 1156 2573 Fragments arranged by size: 2573 1359 1156 388 327 319 247 235 202 101 BmgBI CAC'GTC Cuts at: 1318 2839 1318 Size: 1521 5386 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2549 2709 3057 Size: 416 53 83 399 24 1132 160 348 Cuts at: 3057 4638 5596 442 Size: 1581 958 1753 Fragments arranged by size: 1753 1581 1132 958 416 399 348 160 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6907 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2254 2731 2254 Size: 477 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2291 2437 2475 2612 3527 5567 1239 Size: 1037 15 146 38 137 915 2040 2579 Fragments arranged by size: 2579 2040 1037 915 146 137 38 15 Bpu10I CC'TnA_GC Cuts at: 3119 3119 Size: 6907 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3818 5032 5897 6195 3818 Size: 1214 865 298 4530 Fragments arranged by size: 4530 1214 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3919 5570 882 Size: 617 2420 1651 2219 Fragments arranged by size: 2420 2219 1651 617 BsaAI yAC'GTr Cuts at: 493 1204 4698 493 Size: 711 3494 2702 Fragments arranged by size: 3494 2702 711 BsaBI GATnn'nnATC Cuts at: 6901 6901 Size: 6907 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4714 5096 5731 275 Size: 53 83 186 936 3181 382 635 1451 Fragments arranged by size: 3181 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2145 5340 5738 6012 6159 6570 2145 Size: 3195 398 274 147 411 2482 Fragments arranged by size: 3195 2482 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2467 2497 2619 2649 3221 Size: 30 165 30 1064 30 122 30 572 Cuts at: 3221 3251 1178 Size: 30 4834 Fragments arranged by size: 4834 1064 572 165 122 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 2105 2177 2357 2573 2660 2953 3242 Size: 916 3 72 180 216 87 293 289 Cuts at: 3242 1186 Size: 4851 Fragments arranged by size: 4851 916 293 289 216 180 87 72 3 BseYI C'CCAG_C Cuts at: 2020 2193 2756 2927 3041 4011 6110 6606 2020 Size: 173 563 171 114 970 2099 496 2321 Fragments arranged by size: 2321 2099 970 563 496 173 171 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2115 2336 2435 3181 3484 1163 Size: 573 379 221 99 746 303 4586 Fragments arranged by size: 4586 746 573 379 303 221 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3073 3175 3236 3574 4357 5118 Size: 275 186 1072 102 61 338 783 761 Cuts at: 5118 6146 1540 Size: 1028 2301 Fragments arranged by size: 2301 1072 1028 783 761 338 275 186 102 61 BsiHKAI G_wGCw'C Cuts at: 2041 2730 3306 3513 3609 3933 4169 4968 5053 Size: 689 576 207 96 324 236 799 85 Cuts at: 5053 6124 2041 Size: 1071 2824 Fragments arranged by size: 2824 1071 799 689 576 324 236 207 96 85 BsmI GAATG_Cn' Cuts at: 4418 4511 4418 Size: 93 6814 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2041 2178 2451 2505 2730 2740 Size: 399 237 14 137 273 54 225 10 Cuts at: 2740 2987 3306 3407 3513 3609 3933 4169 4226 Size: 247 319 101 106 96 324 236 57 Cuts at: 4226 4968 5053 6124 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 324 319 273 247 237 236 225 137 106 101 96 85 57 54 14 10 BspEI T'CCGG_A Cuts at: 6570 6570 Size: 6907 BspHI T'CATG_A Cuts at: 4796 6526 4796 Size: 1730 5177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2539 2798 3123 3207 3901 4262 844 Size: 1695 259 325 84 694 361 3489 Fragments arranged by size: 3489 1695 694 361 325 259 84 BsrBI CCG'CTC Cuts at: 1394 1823 3232 3579 3619 3703 4794 1394 Size: 429 1409 347 40 84 1091 3507 Fragments arranged by size: 3507 1409 1091 429 347 84 40 BsrDI GCAATG_nn' Cuts at: 65 1248 5402 5576 65 Size: 1183 4154 174 1396 Fragments arranged by size: 4154 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3597 3681 3863 4017 5551 5738 1700 Size: 110 1787 84 182 154 1534 187 2869 Fragments arranged by size: 2869 1787 1534 187 182 154 110 84 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6907 BssHII G'CGCG_C Cuts at: 1925 3276 3278 3493 1925 Size: 1351 2 215 5339 Fragments arranged by size: 5339 1351 215 2 BssSI C'ACGA_G Cuts at: 4967 5979 4967 Size: 1012 5895 BstAPI GCAn_nnn'nTGC Cuts at: 794 3168 3202 3471 4102 794 Size: 2374 34 269 631 3599 Fragments arranged by size: 3599 2374 631 269 34 BstBI TT'CG_AA Cuts at: 5711 5711 Size: 6907 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6907 BstXI CCAn_nnnn'nTGG Cuts at: 2660 4145 2660 Size: 1485 5422 BstYI r'GATC_y Cuts at: 1972 2301 2679 2877 2965 3132 4237 4604 4989 Size: 329 378 198 88 167 1105 367 385 Cuts at: 4989 5006 6458 6544 6556 6902 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1105 385 378 367 346 329 198 167 88 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2643 2706 2643 Size: 63 6844 BtgI C'CryG_G Cuts at: 513 1065 2955 3388 513 Size: 552 1890 433 4032 Fragments arranged by size: 4032 1890 552 433 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3093 487 Size: 683 56 1867 4301 Fragments arranged by size: 4301 1867 683 56 BtsI GCAGTG_nn' Cuts at: 2288 3880 4423 5217 5237 2288 Size: 1592 543 794 20 3958 Fragments arranged by size: 3958 1592 794 543 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6907 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6872 DraI TTT'AAA Cuts at: 4211 4211 Size: 6907 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5914 809 Size: 507 4598 1802 Fragments arranged by size: 4598 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2327 2872 3070 Size: 54 1425 50 275 18 497 545 198 Cuts at: 3070 3172 3233 3256 3340 3571 3643 3811 3979 Size: 102 61 23 84 231 72 168 168 Cuts at: 3979 4147 4354 5243 6872 8 Size: 168 207 889 1629 43 Fragments arranged by size: 1629 1425 889 545 497 275 231 207 198 168 168 168 102 84 72 61 54 50 43 23 18 EagI C'GGCC_G Cuts at: 1537 1812 3070 3172 3233 3571 4354 1537 Size: 275 1258 102 61 338 783 4090 Fragments arranged by size: 4090 1258 783 338 275 102 61 EarI CTCTTCn'nnn_ Cuts at: 1353 3282 4837 1353 Size: 1929 1555 3423 Fragments arranged by size: 3423 1929 1555 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2400 3728 4064 5494 5868 6014 Size: 1064 138 811 1328 336 1430 374 146 Cuts at: 6014 387 Size: 1280 Fragments arranged by size: 1430 1328 1280 1064 811 374 336 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2145 2276 2291 2437 2475 2612 3166 Size: 393 513 131 15 146 38 137 554 Cuts at: 3166 3527 3805 4970 5567 6354 6622 1239 Size: 361 278 1165 597 787 268 1524 Fragments arranged by size: 1524 1165 787 597 554 513 393 361 278 268 146 137 131 38 15 EcoNI CCTnn'n_nnAGG Cuts at: 2642 2719 2642 Size: 77 6830 EcoO109I rG'GnC_Cy Cuts at: 2057 2113 2120 2174 2175 2185 2428 2711 2736 Size: 56 7 54 1 10 243 283 25 Cuts at: 2736 2737 2848 2950 2984 3404 2057 Size: 1 111 102 34 420 5560 Fragments arranged by size: 5560 420 283 243 111 102 56 54 34 25 10 7 1 1 EcoRI G'AATT_C Cuts at: 4216 4594 4216 Size: 378 6529 FspI TGC'GCA Cuts at: 2910 3272 4106 5413 2910 Size: 362 834 1307 4404 Fragments arranged by size: 4404 1307 834 362 HaeII r_GCGC'y Cuts at: 1678 3013 3361 3414 3568 3664 3820 3832 4203 Size: 1335 348 53 154 96 156 12 371 Cuts at: 4203 6054 1678 Size: 1851 2531 Fragments arranged by size: 2531 1851 1335 371 348 156 154 96 53 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2417 2449 5548 5580 5622 Size: 32 135 32 845 32 3099 32 42 Cuts at: 5622 5654 1373 Size: 32 2626 Fragments arranged by size: 3099 2626 845 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4251 4497 669 Size: 988 281 2313 246 3079 Fragments arranged by size: 3079 2313 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6907 HpaI GTT'AAC Cuts at: 4497 4497 Size: 6907 KpnI G_GTAC'C Cuts at: 4232 4232 Size: 6907 MfeI C'AATT_G Cuts at: 4506 4506 Size: 6907 MluI A'CGCG_T Cuts at: 3842 6783 3842 Size: 2941 3966 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2510 3690 6021 6205 1305 Size: 1205 1180 2331 184 2007 Fragments arranged by size: 2331 2007 1205 1180 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2874 3258 3813 10 Size: 54 1425 343 1042 384 555 3104 Fragments arranged by size: 3104 1425 1042 555 384 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3141 3157 3885 4032 4865 5224 5383 Size: 648 1975 16 728 147 833 359 159 Cuts at: 5383 518 Size: 2042 Fragments arranged by size: 2042 1975 833 728 648 359 159 147 16 MspA1I CmG'CkG Cuts at: 1606 2326 3006 3745 4081 4323 5000 6148 6393 Size: 720 680 739 336 242 677 1148 245 Cuts at: 6393 1606 Size: 2120 Fragments arranged by size: 2120 1148 739 720 680 677 336 245 242 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6355 NdeI CA'TA_TG Cuts at: 387 387 Size: 6907 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6907 NotI GC'GGCC_GC Cuts at: 3172 4354 3172 Size: 1182 5725 NspI r_CATG'y Cuts at: 1132 1229 2915 4053 4265 5810 1132 Size: 97 1686 1138 212 1545 2229 Fragments arranged by size: 2229 1686 1545 1138 212 97 PasI CC'CwG_GG Cuts at: 1348 2733 2774 2844 3018 1348 Size: 1385 41 70 174 5237 Fragments arranged by size: 5237 1385 174 70 41 PciI A'CATG_T Cuts at: 5806 5806 Size: 6907 PflMI CCAn_nnn'nTGG Cuts at: 1108 2498 3006 1108 Size: 1390 508 5009 Fragments arranged by size: 5009 1390 508 PfoI T'CCnGG_A Cuts at: 2589 2589 Size: 6907 PmeI GTTT'AAAC Cuts at: 4211 4211 Size: 6907 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3221 3253 4976 5008 6489 6521 3221 Size: 32 1723 32 1481 32 3607 Fragments arranged by size: 3607 1723 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2113 2428 2711 2848 2950 2113 Size: 315 283 137 102 6070 Fragments arranged by size: 6070 315 283 137 102 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6907 PsiI TTA'TAA Cuts at: 4477 4477 Size: 6907 PspOMI G'GGCC_C Cuts at: 2174 2501 2736 2983 3403 2174 Size: 327 235 247 420 5678 Fragments arranged by size: 5678 420 327 247 235 PstI C_TGCA'G Cuts at: 830 2550 2790 3375 3594 4259 830 Size: 1720 240 585 219 665 3478 Fragments arranged by size: 3478 1720 665 585 240 219 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6907 SalI G'TCGA_C Cuts at: 1936 4249 1936 Size: 2313 4594 SanDI GG'GwC_CC Cuts at: 2113 2711 2113 Size: 598 6309 SapI GCTCTTCn'nnn_ Cuts at: 3282 3282 Size: 6907 SbfI CC_TGCA'GG Cuts at: 2550 4259 2550 Size: 1709 5198 ScaI AGT'ACT Cuts at: 5155 6867 5155 Size: 1712 5195 SfcI C'TryA_G Cuts at: 826 1045 2546 2786 3371 3590 4255 5390 6071 Size: 219 1501 240 585 219 665 1135 681 Cuts at: 6071 6262 826 Size: 191 1471 Fragments arranged by size: 1501 1471 1135 681 665 585 240 219 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2871 2871 Size: 6907 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6907 SgrAI Cr'CCGG_yG Cuts at: 1700 3597 3681 3863 4017 1700 Size: 1897 84 182 154 4590 Fragments arranged by size: 4590 1897 182 154 84 SmaI CCC'GGG Cuts at: 1872 3402 4234 1872 Size: 1530 832 4545 Fragments arranged by size: 4545 1530 832 SmlI C'TyrA_G Cuts at: 820 1017 1943 3833 5011 5912 6174 820 Size: 197 926 1890 1178 901 262 1553 Fragments arranged by size: 1890 1553 1178 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4698 493 Size: 4205 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6907 SphI G_CATG'C Cuts at: 1132 2915 4053 4265 1132 Size: 1783 1138 212 3774 Fragments arranged by size: 3774 1783 1138 212 SspI AAT'ATT Cuts at: 5 52 4831 5 Size: 47 4779 2081 Fragments arranged by size: 4779 2081 47 StuI AGG'CCT Cuts at: 1517 2011 3734 4070 1517 Size: 494 1723 336 4354 Fragments arranged by size: 4354 1723 494 336 StyI C'CwwG_G Cuts at: 513 1065 2241 2516 4346 513 Size: 552 1176 275 1830 3074 Fragments arranged by size: 3074 1830 1176 552 275 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2049 2630 4025 4124 4934 4951 5104 Size: 1255 469 581 1395 99 810 17 153 Cuts at: 5104 5289 5734 325 Size: 185 445 1498 Fragments arranged by size: 1498 1395 1255 810 581 469 445 185 153 99 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5153 6865 96 Size: 274 80 33 51 1443 3176 1712 138 Fragments arranged by size: 3176 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6867 6899 6867 Size: 32 6875 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6907 XbaI T'CTAG_A Cuts at: 4243 4243 Size: 6907 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2696 2696 Size: 6907 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6907 XmaI C'CCGG_G Cuts at: 1870 3400 4232 1870 Size: 1530 832 4545 Fragments arranged by size: 4545 1530 832 XmnI GAAnn'nnTTC Cuts at: 4127 5036 4127 Size: 909 5998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4715 276 Size: 53 83 186 936 3181 2468 Fragments arranged by size: 3181 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SexAI SfoI SrfI SwaI