(Linear) (Six-base) MAPSORT of: KIBB6220.seq Check: 8320 from: 1 to: 2202 KIBB6220.orf With 182 enzymes: SgfI * July 3, 2012 16:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 534 1202 2202 Size: 534 668 1000 Fragments arranged by size: 1000 668 534 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 140 1161 1800 2202 Size: 140 1021 639 402 Fragments arranged by size: 1021 639 402 140 AfeI AGC'GCT Cuts at: 0 1354 1561 1657 1813 2202 Size: 1354 207 96 156 389 Fragments arranged by size: 1354 389 207 156 96 AflIII A'CryG_T Cuts at: 0 1837 2202 Size: 1837 365 AleI CACnn'nnGTG Cuts at: 0 1136 1880 2027 2202 Size: 1136 744 147 175 Fragments arranged by size: 1136 744 175 147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1126 2202 Size: 1126 1076 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1216 1248 1301 1333 2202 Size: 1216 32 53 32 869 Fragments arranged by size: 1216 869 53 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 118 216 249 276 493 2097 2202 Size: 118 98 33 27 217 1604 105 Fragments arranged by size: 1604 217 118 105 98 33 27 ApaI G_GGCC'C Cuts at: 0 173 500 735 982 1402 2202 Size: 173 327 235 247 420 800 Fragments arranged by size: 800 420 327 247 235 173 ApaLI G'TGCA_C Cuts at: 0 1297 1600 2202 Size: 1297 303 602 Fragments arranged by size: 1297 602 303 AscI GG'CGCG_CC Cuts at: 0 1488 2202 Size: 1488 714 AvaI C'yCGr_G Cuts at: 0 334 1395 1435 1798 1966 2202 Size: 334 1061 40 363 168 236 Fragments arranged by size: 1061 363 334 236 168 40 AvrII C'CTAG_G Cuts at: 0 511 2202 Size: 511 1691 BamHI G'GATC_C Cuts at: 0 960 2202 Size: 960 1242 BanI G'GyrC_C Cuts at: 0 1118 2202 Size: 1118 1084 BanII G_rGCy'C Cuts at: 0 22 173 446 500 735 982 1402 2202 Size: 22 151 273 54 235 247 420 800 Fragments arranged by size: 800 420 273 247 235 151 54 22 BbvCI CC'TCA_GC Cuts at: 0 1114 2202 Size: 1114 1088 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 325 359 2202 Size: 325 34 1843 Fragments arranged by size: 1843 325 34 BclI T'GATC_A Cuts at: 0 989 2202 Size: 989 1213 BglI GCCn_nnn'nGGC Cuts at: 0 866 2202 Size: 866 1336 BglII A'GATC_T Cuts at: 0 674 2202 Size: 674 1528 BlpI GC'TnA_GC Cuts at: 0 1409 2202 Size: 1409 793 Bme1580I G_kGCm'C Cuts at: 0 173 500 735 982 1301 1402 1604 2202 Size: 173 327 235 247 319 101 202 598 Fragments arranged by size: 598 327 319 247 235 202 173 101 BmgBI CAC'GTC Cuts at: 0 834 2202 Size: 834 1368 BmrI ACTGGGnnnn_n' Cuts at: 0 544 704 1052 2202 Size: 544 160 348 1150 Fragments arranged by size: 1150 544 348 160 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 249 726 2202 Size: 249 477 1476 Fragments arranged by size: 1476 477 249 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 286 432 470 607 1522 2202 Size: 271 15 146 38 137 915 680 Fragments arranged by size: 915 680 271 146 137 38 15 Bpu10I CC'TnA_GC Cuts at: 0 1114 2202 Size: 1114 1088 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1813 2202 Size: 1813 389 BsaI GGTCTCn'nnnn_ Cuts at: 0 1914 2202 Size: 1914 288 BsaWI w'CCGG_w Cuts at: 0 140 2202 Size: 140 2062 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 462 492 614 644 1216 1246 2202 Size: 462 30 122 30 572 30 956 Fragments arranged by size: 956 572 462 122 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 97 100 172 352 568 655 948 1237 Size: 97 3 72 180 216 87 293 289 Cuts at: 1237 2202 Size: 965 Fragments arranged by size: 965 293 289 216 180 97 87 72 3 BseYI C'CCAG_C Cuts at: 0 15 188 751 922 1036 2006 2202 Size: 15 173 563 171 114 970 196 Fragments arranged by size: 970 563 196 173 171 114 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 331 430 1176 1479 2202 Size: 110 221 99 746 303 723 Fragments arranged by size: 746 723 303 221 110 99 BsiEI CG_ry'CG Cuts at: 0 1068 1170 1231 1569 2202 Size: 1068 102 61 338 633 Fragments arranged by size: 1068 633 338 102 61 BsiHKAI G_wGCw'C Cuts at: 0 36 725 1301 1508 1604 1928 2164 2202 Size: 36 689 576 207 96 324 236 38 Fragments arranged by size: 689 576 324 236 207 96 38 36 Bsp1286I G_dGCh'C Cuts at: 0 22 36 173 446 500 725 735 982 Size: 22 14 137 273 54 225 10 247 Cuts at: 982 1301 1402 1508 1604 1928 2164 2202 Size: 319 101 106 96 324 236 38 Fragments arranged by size: 324 319 273 247 236 225 137 106 101 96 54 38 22 14 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 534 793 1118 1202 1896 2202 Size: 534 259 325 84 694 306 Fragments arranged by size: 694 534 325 306 259 84 BsrBI CCG'CTC Cuts at: 0 1227 1574 1614 1698 2202 Size: 1227 347 40 84 504 Fragments arranged by size: 1227 504 347 84 40 BsrFI r'CCGG_y Cuts at: 0 1592 1676 1858 2012 2202 Size: 1592 84 182 154 190 Fragments arranged by size: 1592 190 182 154 84 BssHII G'CGCG_C Cuts at: 0 1271 1273 1488 2202 Size: 1271 2 215 714 Fragments arranged by size: 1271 714 215 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 1163 1197 1466 2097 2202 Size: 1163 34 269 631 105 Fragments arranged by size: 1163 631 269 105 34 BstXI CCAn_nnnn'nTGG Cuts at: 0 655 2140 2202 Size: 655 1485 62 Fragments arranged by size: 1485 655 62 BstYI r'GATC_y Cuts at: 0 296 674 872 960 1127 2202 Size: 296 378 198 88 167 1075 Fragments arranged by size: 1075 378 296 198 167 88 Bsu36I CC'TnA_GG Cuts at: 0 638 701 2202 Size: 638 63 1501 Fragments arranged by size: 1501 638 63 BtgI C'CryG_G Cuts at: 0 950 1383 2202 Size: 950 433 819 Fragments arranged by size: 950 819 433 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1088 2202 Size: 1088 1114 BtsI GCAGTG_nn' Cuts at: 0 283 1875 2202 Size: 283 1592 327 Fragments arranged by size: 1592 327 283 EaeI y'GGCC_r Cuts at: 0 322 867 1065 1167 1228 1251 1335 1566 Size: 322 545 198 102 61 23 84 231 Cuts at: 1566 1638 1806 1974 2142 2202 Size: 72 168 168 168 60 Fragments arranged by size: 545 322 231 198 168 168 168 102 84 72 61 60 23 EagI C'GGCC_G Cuts at: 0 1065 1167 1228 1566 2202 Size: 1065 102 61 338 636 Fragments arranged by size: 1065 636 338 102 61 EarI CTCTTCn'nnn_ Cuts at: 0 1277 2202 Size: 1277 925 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 395 1723 2059 2202 Size: 395 1328 336 143 Fragments arranged by size: 1328 395 336 143 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 140 271 286 432 470 607 1161 1522 Size: 140 131 15 146 38 137 554 361 Cuts at: 1522 1800 2202 Size: 278 402 Fragments arranged by size: 554 402 361 278 146 140 137 131 38 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 637 714 2202 Size: 637 77 1488 Fragments arranged by size: 1488 637 77 EcoO109I rG'GnC_Cy Cuts at: 0 52 108 115 169 170 180 423 706 Size: 52 56 7 54 1 10 243 283 Cuts at: 706 731 732 843 945 979 1399 2202 Size: 25 1 111 102 34 420 803 Fragments arranged by size: 803 420 283 243 111 102 56 54 52 34 25 10 7 1 1 FspI TGC'GCA Cuts at: 0 905 1267 2101 2202 Size: 905 362 834 101 Fragments arranged by size: 905 834 362 101 HaeII r_GCGC'y Cuts at: 0 1008 1356 1409 1563 1659 1815 1827 2198 Size: 1008 348 53 154 96 156 12 371 Cuts at: 2198 2202 Size: 4 Fragments arranged by size: 1008 371 348 156 154 96 53 12 4 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 412 444 2202 Size: 412 32 1758 Fragments arranged by size: 1758 412 32 MluI A'CGCG_T Cuts at: 0 1837 2202 Size: 1837 365 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 505 1685 2202 Size: 505 1180 517 Fragments arranged by size: 1180 517 505 MscI TGG'CCA Cuts at: 0 869 1253 1808 2202 Size: 869 384 555 394 Fragments arranged by size: 869 555 394 384 MslI CAynn'nnrTG Cuts at: 0 1136 1152 1880 2027 2202 Size: 1136 16 728 147 175 Fragments arranged by size: 1136 728 175 147 16 MspA1I CmG'CkG Cuts at: 0 321 1001 1740 2076 2202 Size: 321 680 739 336 126 Fragments arranged by size: 739 680 336 321 126 NotI GC'GGCC_GC Cuts at: 0 1167 2202 Size: 1167 1035 NspI r_CATG'y Cuts at: 0 910 2048 2202 Size: 910 1138 154 Fragments arranged by size: 1138 910 154 PasI CC'CwG_GG Cuts at: 0 728 769 839 1013 2202 Size: 728 41 70 174 1189 Fragments arranged by size: 1189 728 174 70 41 PflMI CCAn_nnn'nTGG Cuts at: 0 493 1001 2202 Size: 493 508 1201 Fragments arranged by size: 1201 508 493 PfoI T'CCnGG_A Cuts at: 0 584 2202 Size: 584 1618 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1216 1248 2202 Size: 1216 32 954 Fragments arranged by size: 1216 954 32 PpuMI rG'GwC_Cy Cuts at: 0 108 423 706 843 945 2202 Size: 108 315 283 137 102 1257 Fragments arranged by size: 1257 315 283 137 108 102 PspOMI G'GGCC_C Cuts at: 0 169 496 731 978 1398 2202 Size: 169 327 235 247 420 804 Fragments arranged by size: 804 420 327 247 235 169 PstI C_TGCA'G Cuts at: 0 545 785 1370 1589 2202 Size: 545 240 585 219 613 Fragments arranged by size: 613 585 545 240 219 SanDI GG'GwC_CC Cuts at: 0 108 706 2202 Size: 108 598 1496 Fragments arranged by size: 1496 598 108 SapI GCTCTTCn'nnn_ Cuts at: 0 1277 2202 Size: 1277 925 SbfI CC_TGCA'GG Cuts at: 0 545 2202 Size: 545 1657 SfcI C'TryA_G Cuts at: 0 541 781 1366 1585 2202 Size: 541 240 585 219 617 Fragments arranged by size: 617 585 541 240 219 SfiI GGCCn_nnn'nGGCC Cuts at: 0 866 2202 Size: 866 1336 SgrAI Cr'CCGG_yG Cuts at: 0 1592 1676 1858 2012 2202 Size: 1592 84 182 154 190 Fragments arranged by size: 1592 190 182 154 84 SmaI CCC'GGG Cuts at: 0 1397 2202 Size: 1397 805 SmlI C'TyrA_G Cuts at: 0 1828 2202 Size: 1828 374 SphI G_CATG'C Cuts at: 0 910 2048 2202 Size: 910 1138 154 Fragments arranged by size: 1138 910 154 StuI AGG'CCT Cuts at: 0 6 1729 2065 2202 Size: 6 1723 336 137 Fragments arranged by size: 1723 336 137 6 StyI C'CwwG_G Cuts at: 0 236 511 2202 Size: 236 275 1691 Fragments arranged by size: 1691 275 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 44 625 2020 2119 2202 Size: 44 581 1395 99 83 Fragments arranged by size: 1395 581 99 83 44 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 691 2202 Size: 691 1511 XmaI C'CCGG_G Cuts at: 0 1395 2202 Size: 1395 807 XmnI GAAnn'nnTTC Cuts at: 0 2122 2202 Size: 2122 80 Enzymes that do cut: AarI AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaI ApaLI AscI AvaI AvrII BamHI BanI BanII BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I MluI MmeI MscI MslI MspA1I NotI NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI SanDI SapI SbfI SfcI SfiI SgrAI SmaI SmlI SphI StuI StyI TaqII XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI ApoI AseI AsiSI BaeI BbeI BbsI BciVI BfrBI BmtI BsaAI BsaBI BsaHI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI ScaI SexAI SfoI SgfI SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI ZraI