Restriction map for
pFN21AE3238
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# NO CUT ENZYMES ( 119 enzymes) #
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Acc65I AccII AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BglI BglII BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
BspEI BspHI BspLU11I BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I CpoI DraI DraII DraIII
DsaI Eam1105I Eco47III Eco81I EcoO109I
EcoO65I EcoRV EcoT22I FseI FspI
HgaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NcoI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
RcaI RsrII SacII SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 913 GACGTC GACGT^C
AccI 910 GTCGAC GT^[AC][GT]AC
AseI 421 ATTAAT AT^TAAT
AsnI 421 ATTAAT AT^TAAT
AvaI 223 CCCGAG C^[CT]CG[GA]G
AvaII 1527 GGTCC G^G[AT]CC
BanII 737 GAGCTC G[GA]GC[CT]^C
BbeI 60 GGCGCC GGCGC^C
BclI 1167 TGATCA T^GATCA
BlpI 186 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 186 GCTGAGC GC^T[ACGT]AGC
BsgI 918 CTGCAC GTGCAG(16/14)
BsiYI 5 CCTGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 5 CCTGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 1384 GAGAC GTCTC(1/5)
BsmI 747 GAATGC GAATGC(1/-1)
BsoBI 223 CCCGAG C^[CT]CG[GA]G
Bsp1407I 900 TGTACA T^GTACA
BspDI 1010 ATCGAT AT^CGAT
BsrBI 130 GAGCGG CCGCTC(-3/-3)
BsrDI 878 CATTGC GCAATG(2/0)
BsrGI 900 TGTACA T^GTACA
BstXI 827 CCAATGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 186 GCTGAGC GC^T[ACGT]AGC
ClaI 1010 ATCGAT AT^CGAT
DrdI 1550 GACTTATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 5 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 402 GAATTC G^AATTC
FbaI 1167 TGATCA T^GATCA
HpaI 96 GTTAAC GTT^AAC
KasI 60 GGCGCC G^GCGCC
NarI 60 GGCGCC GG^CGCC
NdeI 447 CATATG CA^TATG
NheI 706 GCTAGC G^CTAGC
NspI 897 GCATGT [GA]CATG^[CT]
PleI 777 GACTC GAGTC(4/5)
PshBI 421 ATTAAT AT^TAAT
PvuII 904 CAGCTG CAG^CTG
SacI 737 GAGCTC GAGCT^C
SalI 910 GTCGAC G^TCGAC
SfoI 60 GGCGCC GGC^GCC
SspBI 900 TGTACA T^GTACA
SspI 471 AATATT AAT^ATT
XcmI 663 CCAGGGATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcyI 60 GGCGCC G[GA]^CG[CT]C
913 GACGTC
AfaI 344 GTAC GT^AC
901 GTAC
AflII 646 CTTAAG C^TTAAG
1069 CTTAAG
Asp700I 1304 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1582 GAAATGATTC
BbsI 433 GAAGAC GAAGAC(2/6)
1078 GAAGAC
BbvI 108 GCAGC GCAGC(8/12)
73 GCTGC
BfaI 707 CTAG C^TAG
1244 CTAG
BfrI 646 CTTAAG C^TTAAG
1069 CTTAAG
BpuAI 433 GAAGAC GAAGAC(2/6)
1078 GAAGAC
BsaHI 60 GGCGCC G[GA]^CG[CT]C
913 GACGTC
BsiEI 45 CGGCCG CG[GA][CT]^CG
125 CGGCCG
Cfr13I 212 GGCCC G^G[ACGT]CC
1527 GGTCC
EagI 45 CGGCCG C^GGCCG
125 CGGCCG
EclXI 45 CGGCCG C^GGCCG
125 CGGCCG
Eco52I 45 CGGCCG C^GGCCG
125 CGGCCG
EcoT14I 1417 CCAAGG C^C[AT][AT]GG
1486 CCTTGG
FauI 105 CCCGC CCCGC(4/6)
235 GCGGG
Hin1I 60 GGCGCC G[GA]^CG[CT]C
913 GACGTC
HincII 96 GTTAAC GT[CT]^[GA]AC
910 GTCGAC
HindII 96 GTTAAC GT[CT]^[GA]AC
910 GTCGAC
HindIII 583 AAGCTT A^AGCTT
940 AAGCTT
MaeI 707 CTAG C^TAG
1244 CTAG
MslI 200 CACTGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
885 CATTATAATG
PshAI 494 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
777 GACTCCTGTC
RsaI 344 GTAC GT^AC
901 GTAC
Sau96I 212 GGCCC G^G[ACGT]CC
1527 GGTCC
SmlI 646 CTTAAG C^T[CT][GA]AG
1069 CTTAAG
StyI 1417 CCAAGG C^C[AT][AT]GG
1486 CCTTGG
TseI 73 GCTGC G^C[AT]GC
108 GCAGC
XmnI 1304 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1582 GAAATGATTC
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI BanI BcnI BmyI BpmI
BsaJI BseRI BsiHKAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI DdeI DpnII EaeI
EarI EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
ScrFI SfaNI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I XhoII