Restriction map for
pFN21AE3238
(Circular) (Six-base) MAPSORT of: pFN21AE3238.seq Check: 2657 from: 1 to: 6301
pFN21AE3238 6301 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2922 4111 278
Size: 53 83 186 936 1386 1189 2468
Fragments arranged by size:
2468 1386 1189 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2916 3644 1937
Size: 979 728 4594
Fragments arranged by size:
4594 979 728
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2914 3459 4364 5748 6016 1632
Size: 1282 545 905 1384 268 1917
Fragments arranged by size:
1917 1384 1282 905 545 268
AflII C'TTAA_G
Cuts at: 820 1017 2651 3074 820
Size: 197 1634 423 4047
Fragments arranged by size:
4047 1634 423 197
AflIII A'CryG_T
Cuts at: 1203 5200 6177 1203
Size: 3997 977 1327
Fragments arranged by size:
3997 1327 977
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 2209 1166
Size: 1043 5258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3244 1184
Size: 2060 4241
AlwNI CAG_nnn'CTG
Cuts at: 4044 5616 4044
Size: 1572 4729
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 2276 2407 3326 3610 3836 3988 6097 1493
Size: 783 131 919 284 226 152 2109 1697
Fragments arranged by size:
2109 1697 919 783 284 226 152 131
AseI AT'TA_AT
Cuts at: 160 1032 2427 4855 160
Size: 872 1395 2428 1606
Fragments arranged by size:
2428 1606 1395 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 2228 3626 1870
Size: 73 285 1398 4545
Fragments arranged by size:
4545 1398 285 73
BamHI G'GATC_C
Cuts at: 3631 3998 3631
Size: 367 5934
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 3244 3259 3622 5077
Size: 325 217 627 278 1179 15 363 1455
Cuts at: 5077 618
Size: 1842
Fragments arranged by size:
1842 1455 1179 627 363 325 278 217
15
BanII G_rGCy'C
Cuts at: 1391 2746 3620 1391
Size: 1355 874 4072
Fragments arranged by size:
4072 1355 874
BbeI G_GCGC'C
Cuts at: 2069 2069
Size: 6301
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2445 3090 928
Size: 971 546 645 4139
Fragments arranged by size:
4139 971 645 546
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4492 4526 1903
Size: 34 50 34 2471 34 3678
Fragments arranged by size:
3678 2471 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4193 5403 4193
Size: 1210 5091
BclI T'GATC_A
Cuts at: 1548 3172 1548
Size: 1624 4677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4912 136
Size: 107 122 71 631 605 3240 1525
Fragments arranged by size:
3240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6296 6296
Size: 6301
BlpI GC'TnA_GC
Cuts at: 2192 3718 2192
Size: 1526 4775
Bme1580I G_kGCm'C
Cuts at: 1790 2041 2637 3145 4362 5518 1790
Size: 251 596 508 1217 1156 2573
Fragments arranged by size:
2573 1217 1156 596 508 251
BmgBI CAC'GTC
Cuts at: 1318 2185 1318
Size: 867 5434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4032 4990 442
Size: 416 53 83 399 24 2615 958 1753
Fragments arranged by size:
2615 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2715 1056
Size: 1659 4642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 2063 2651 4961 1239
Size: 793 31 588 2310 2579
Fragments arranged by size:
2579 2310 793 588 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4426 5291 5589 4426
Size: 865 298 5138
Fragments arranged by size:
5138 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4964 882
Size: 617 3465 2219
Fragments arranged by size:
3465 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4092 493
Size: 711 2888 2702
Fragments arranged by size:
2888 2702 711
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2919 4108 4490
Size: 53 83 186 936 533 853 1189 382
Cuts at: 4490 5125 275
Size: 635 1451
Fragments arranged by size:
1451 1189 936 853 635 533 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4734 5132 5406 5553 5964 4734
Size: 398 274 147 411 5071
Fragments arranged by size:
5071 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2161 2191 1178
Size: 30 165 30 758 30 5288
Fragments arranged by size:
5288 758 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 3467 3470 3473 1186
Size: 843 1438 3 3 4014
Fragments arranged by size:
4014 1438 843 3 3
BseYI C'CCAG_C
Cuts at: 2756 5504 6000 2756
Size: 2748 496 3057
Fragments arranged by size:
3057 2748 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2908 1163
Size: 573 1172 4556
Fragments arranged by size:
4556 1172 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2053 2133 3751 4512 5540 1540
Size: 275 186 52 80 1618 761 1028 2301
Fragments arranged by size:
2301 1618 1028 761 275 186 80 52
BsiHKAI G_wGCw'C
Cuts at: 2206 2746 2797 4362 4447 5518 2206
Size: 540 51 1565 85 1071 2989
Fragments arranged by size:
2989 1565 1071 540 85 51
BsmI GAATG_Cn'
Cuts at: 2758 3812 3905 2758
Size: 1054 93 5154
Fragments arranged by size:
5154 1054 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2206 2637 2746 2797 3145 3620
Size: 399 251 165 431 109 51 348 475
Cuts at: 3620 4362 4447 5518 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 475 431 399 348 251
165 109 85 51
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 4190 5920 4190
Size: 1730 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3656 844
Size: 2812 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2137 4188 1394
Size: 429 314 2051 3507
Fragments arranged by size:
3507 2051 429 314
BsrDI GCAATG_nn'
Cuts at: 65 1248 2882 4796 4970 65
Size: 1183 1634 1914 174 1396
Fragments arranged by size:
1914 1634 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4945 5132 1700
Size: 110 3135 187 2869
Fragments arranged by size:
3135 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2905 96
Size: 2809 3492
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 4361 5373 4361
Size: 1012 5289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstXI CCAn_nnnn'nTGG
Cuts at: 2839 2839
Size: 6301
BstYI r'GATC_y
Cuts at: 1972 2705 2825 3149 3631 3998 4383 4400 5852
Size: 733 120 324 482 367 385 17 1452
Cuts at: 5852 5938 5950 6296 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 733 482 385 367 346 324
120 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5562
Fragments arranged by size:
5562 683 56
BtsI GCAGTG_nn'
Cuts at: 2204 2645 3817 4611 4631 2204
Size: 441 1172 794 20 3874
Fragments arranged by size:
3874 1172 794 441 20
ClaI AT'CG_AT
Cuts at: 1554 3016 1554
Size: 1462 4839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6266
DraI TTT'AAA
Cuts at: 3605 3605
Size: 6301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3561 5308 809
Size: 507 2245 1747 1802
Fragments arranged by size:
2245 1802 1747 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2130 3320
Size: 54 1425 50 275 18 220 80 1190
Cuts at: 3320 3748 4637 6266 8
Size: 428 889 1629 43
Fragments arranged by size:
1629 1425 1190 889 428 275 220 80
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2050 2130 3748 1537
Size: 275 238 80 1618 4090
Fragments arranged by size:
4090 1618 275 238 80
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 2375 2856 4231 1353
Size: 702 320 481 1375 3423
Fragments arranged by size:
3423 1375 702 481 320
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4888 5262 5408 387
Size: 1064 138 3299 374 146 1280
Fragments arranged by size:
3299 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2744 2744
Size: 6301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2063 2651 2914 3459 4364 4961
Size: 393 400 31 588 263 545 905 597
Cuts at: 4961 5748 6016 1239
Size: 787 268 1524
Fragments arranged by size:
1524 905 787 597 588 545 400 393
268 263 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2014 2014
Size: 6301
EcoRI G'AATT_C
Cuts at: 2407 3610 3988 2407
Size: 1203 378 4720
Fragments arranged by size:
4720 1203 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 2367 2275
Size: 92 6209
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 2069 2099 2236 5448 1678
Size: 391 30 137 3212 2531
Fragments arranged by size:
3212 2531 391 137 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 3178 3210 4942
Size: 32 135 32 713 32 861 32 1732
Cuts at: 4942 4974 5016 5048 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1732 861 713 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2103 2917 3645 3891 669
Size: 988 281 165 814 728 246 3079
Fragments arranged by size:
3079 988 814 728 281 246 165
HindIII A'AGCT_T
Cuts at: 748 2588 2945 748
Size: 1840 357 4104
Fragments arranged by size:
4104 1840 357
HpaI GTT'AAC
Cuts at: 2103 3891 2103
Size: 1788 4513
KasI G'GCGC_C
Cuts at: 2065 2065
Size: 6301
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 3900 3900
Size: 6301
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5415 5599 1305
Size: 4110 184 2007
Fragments arranged by size:
4110 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4479
Fragments arranged by size:
4479 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2209 2894 4259 4618 4777 518
Size: 648 1043 685 1365 359 159 2042
Fragments arranged by size:
2042 1365 1043 685 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2911 2939 3717 4394 5542 5787 1606
Size: 478 827 28 778 677 1148 245 2120
Fragments arranged by size:
2120 1148 827 778 677 478 245 28
NarI GG'CG_CC
Cuts at: 2066 2066
Size: 6301
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5749
NdeI CA'TA_TG
Cuts at: 387 2453 387
Size: 2066 4235
NheI G'CTAG_C
Cuts at: 1052 2711 1052
Size: 1659 4642
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 2906 3659 5204 1132
Size: 97 1677 753 1545 2229
Fragments arranged by size:
2229 1677 1545 753 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6301
PciI A'CATG_T
Cuts at: 5200 5200
Size: 6301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5399
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 3015 4370 4402 5883 5915 2983
Size: 32 1355 32 1481 32 3369
Fragments arranged by size:
3369 1481 1355 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2503 2786 1613
Size: 890 283 5128
Fragments arranged by size:
5128 890 283
PsiI TTA'TAA
Cuts at: 2894 3299 3332 3871 2894
Size: 405 33 539 5324
Fragments arranged by size:
5324 539 405 33
PstI C_TGCA'G
Cuts at: 830 3653 830
Size: 2823 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
PvuII CAG'CTG
Cuts at: 2911 2911
Size: 6301
SacI G_AGCT'C
Cuts at: 2746 2746
Size: 6301
SalI G'TCGA_C
Cuts at: 1936 2915 3643 1936
Size: 979 728 4594
Fragments arranged by size:
4594 979 728
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 4549 6261 4549
Size: 1712 4589
SfcI C'TryA_G
Cuts at: 826 1045 3649 4784 5465 5656 826
Size: 219 2604 1135 681 191 1471
Fragments arranged by size:
2604 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2067 2067
Size: 6301
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3628 1872
Size: 1756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2651 3074 4405 5306 5568 820
Size: 197 926 708 423 1331 901 262 1553
Fragments arranged by size:
1553 1331 926 901 708 423 262 197
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 2478 4225 5
Size: 47 2426 1747 2081
Fragments arranged by size:
2426 2081 1747 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6301
StyI C'CwwG_G
Cuts at: 513 1065 3422 3491 3740 513
Size: 552 2357 69 249 3074
Fragments arranged by size:
3074 2357 552 249 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2515 2787 4328 4345 4498 4683 5128
Size: 1255 935 272 1541 17 153 185 445
Cuts at: 5128 325
Size: 1498
Fragments arranged by size:
1541 1498 1255 935 445 272 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2905 4547 6259
Size: 274 80 33 51 1443 928 1642 1712
Cuts at: 6259 96
Size: 138
Fragments arranged by size:
1712 1642 1443 928 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6261 6293 6261
Size: 32 6269
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2675 2675
Size: 6301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 3626 1870
Size: 1756 4545
XmnI GAAnn'nnTTC
Cuts at: 3313 3591 4430 3313
Size: 278 839 5184
Fragments arranged by size:
5184 839 278
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2920 4109 276
Size: 53 83 186 936 1386 1189 2468
Fragments arranged by size:
2468 1386 1189 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI