(Linear) (Six-base) MAPSORT of: KIEE3238.seq Check: 7909 from: 1 to: 1596 KIEE3238.orf With 182 enzymes: SgfI * July 3, 2012 17:30 .. AatII G_ACGT'C Cuts at: 0 917 1596 Size: 917 679 AccI GT'mk_AC Cuts at: 0 911 1596 Size: 911 685 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 909 1454 1596 Size: 909 545 142 Fragments arranged by size: 909 545 142 AflII C'TTAA_G Cuts at: 0 646 1069 1596 Size: 646 423 527 Fragments arranged by size: 646 527 423 AleI CACnn'nnGTG Cuts at: 0 204 1596 Size: 204 1392 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1239 1596 Size: 1239 357 ApoI r'AATT_y Cuts at: 0 271 402 1321 1596 Size: 271 131 919 275 Fragments arranged by size: 919 275 271 131 AseI AT'TA_AT Cuts at: 0 422 1596 Size: 422 1174 AvaI C'yCGr_G Cuts at: 0 223 1596 Size: 223 1373 BanI G'GyrC_C Cuts at: 0 60 1239 1254 1596 Size: 60 1179 15 342 Fragments arranged by size: 1179 342 60 15 BanII G_rGCy'C Cuts at: 0 741 1596 Size: 741 855 BbeI G_GCGC'C Cuts at: 0 64 1596 Size: 64 1532 BbsI GAAGACnn'nnnn_ Cuts at: 0 440 1085 1596 Size: 440 645 511 Fragments arranged by size: 645 511 440 BclI T'GATC_A Cuts at: 0 1167 1596 Size: 1167 429 BlpI GC'TnA_GC Cuts at: 0 187 1596 Size: 187 1409 Bme1580I G_kGCm'C Cuts at: 0 36 632 1140 1596 Size: 36 596 508 456 Fragments arranged by size: 596 508 456 36 BmgBI CAC'GTC Cuts at: 0 180 1596 Size: 180 1416 BmtI G_CTAG'C Cuts at: 0 710 1596 Size: 710 886 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 58 646 1596 Size: 27 31 588 950 Fragments arranged by size: 950 588 31 27 BsaHI Gr'CG_yC Cuts at: 0 61 914 1596 Size: 61 853 682 Fragments arranged by size: 853 682 61 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 156 186 1596 Size: 156 30 1410 Fragments arranged by size: 1410 156 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 24 1462 1465 1468 1596 Size: 24 1438 3 3 128 Fragments arranged by size: 1438 128 24 3 3 BseYI C'CCAG_C Cuts at: 0 751 1596 Size: 751 845 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 903 1596 Size: 903 693 BsiEI CG_ry'CG Cuts at: 0 48 128 1596 Size: 48 80 1468 Fragments arranged by size: 1468 80 48 BsiHKAI G_wGCw'C Cuts at: 0 201 741 792 1596 Size: 201 540 51 804 Fragments arranged by size: 804 540 201 51 BsmI GAATG_Cn' Cuts at: 0 753 1596 Size: 753 843 Bsp1286I G_dGCh'C Cuts at: 0 36 201 632 741 792 1140 1596 Size: 36 165 431 109 51 348 456 Fragments arranged by size: 456 431 348 165 109 51 36 BsrBI CCG'CTC Cuts at: 0 132 1596 Size: 132 1464 BsrDI GCAATG_nn' Cuts at: 0 877 1596 Size: 877 719 BsrGI T'GTAC_A Cuts at: 0 900 1596 Size: 900 696 BstXI CCAn_nnnn'nTGG Cuts at: 0 834 1596 Size: 834 762 BstYI r'GATC_y Cuts at: 0 700 820 1144 1596 Size: 700 120 324 452 Fragments arranged by size: 700 452 324 120 BtsI GCAGTG_nn' Cuts at: 0 199 640 1596 Size: 199 441 956 Fragments arranged by size: 956 441 199 ClaI AT'CG_AT Cuts at: 0 1011 1596 Size: 1011 585 DrdI GACnn_nn'nnGTC Cuts at: 0 1556 1596 Size: 1556 40 EaeI y'GGCC_r Cuts at: 0 45 125 1315 1596 Size: 45 80 1190 281 Fragments arranged by size: 1190 281 80 45 EagI C'GGCC_G Cuts at: 0 45 125 1596 Size: 45 80 1471 Fragments arranged by size: 1471 80 45 EarI CTCTTCn'nnn_ Cuts at: 0 50 370 851 1596 Size: 50 320 481 745 Fragments arranged by size: 745 481 320 50 EcoICRI GAG'CTC Cuts at: 0 739 1596 Size: 739 857 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 58 646 909 1454 1596 Size: 27 31 588 263 545 142 Fragments arranged by size: 588 545 263 142 31 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 9 1596 Size: 9 1587 EcoRI G'AATT_C Cuts at: 0 402 1596 Size: 402 1194 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 270 362 1596 Size: 270 92 1234 Fragments arranged by size: 1234 270 92 HaeII r_GCGC'y Cuts at: 0 64 94 231 1596 Size: 64 30 137 1365 Fragments arranged by size: 1365 137 64 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 280 312 1173 1205 1596 Size: 280 32 861 32 391 Fragments arranged by size: 861 391 280 32 32 HincII GTy'rAC Cuts at: 0 98 912 1596 Size: 98 814 684 Fragments arranged by size: 814 684 98 HindIII A'AGCT_T Cuts at: 0 583 940 1596 Size: 583 357 656 Fragments arranged by size: 656 583 357 HpaI GTT'AAC Cuts at: 0 98 1596 Size: 98 1498 KasI G'GCGC_C Cuts at: 0 60 1596 Size: 60 1536 MslI CAynn'nnrTG Cuts at: 0 204 889 1596 Size: 204 685 707 Fragments arranged by size: 707 685 204 MspA1I CmG'CkG Cuts at: 0 79 906 934 1596 Size: 79 827 28 662 Fragments arranged by size: 827 662 79 28 NarI GG'CG_CC Cuts at: 0 61 1596 Size: 61 1535 NdeI CA'TA_TG Cuts at: 0 448 1596 Size: 448 1148 NheI G'CTAG_C Cuts at: 0 706 1596 Size: 706 890 NspI r_CATG'y Cuts at: 0 901 1596 Size: 901 695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 978 1010 1596 Size: 978 32 586 Fragments arranged by size: 978 586 32 PshAI GACnn'nnGTC Cuts at: 0 498 781 1596 Size: 498 283 815 Fragments arranged by size: 815 498 283 PsiI TTA'TAA Cuts at: 0 889 1294 1327 1596 Size: 889 405 33 269 Fragments arranged by size: 889 405 269 33 PvuII CAG'CTG Cuts at: 0 906 1596 Size: 906 690 SacI G_AGCT'C Cuts at: 0 741 1596 Size: 741 855 SalI G'TCGA_C Cuts at: 0 910 1596 Size: 910 686 SfoI GGC'GCC Cuts at: 0 62 1596 Size: 62 1534 SmlI C'TyrA_G Cuts at: 0 646 1069 1596 Size: 646 423 527 Fragments arranged by size: 646 527 423 SspI AAT'ATT Cuts at: 0 473 1596 Size: 473 1123 StyI C'CwwG_G Cuts at: 0 1417 1486 1596 Size: 1417 69 110 Fragments arranged by size: 1417 110 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 510 782 1596 Size: 510 272 814 Fragments arranged by size: 814 510 272 TatI w'GTAC_w Cuts at: 0 900 1596 Size: 900 696 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 670 1596 Size: 670 926 XmnI GAAnn'nnTTC Cuts at: 0 1308 1586 1596 Size: 1308 278 10 Fragments arranged by size: 1308 278 10 ZraI GAC'GTC Cuts at: 0 915 1596 Size: 915 681 Enzymes that do cut: AatII AccI AcuI AflII AleI AlfI ApoI AseI AvaI BanI BanII BbeI BbsI BclI BlpI Bme1580I BmgBI BmtI BpmI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrGI BstXI BstYI BtsI ClaI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoRI FalI HaeII Hin4I HincII HindIII HpaI KasI MslI MspA1I NarI NdeI NheI NspI PpiI PshAI PsiI PvuII SacI SalI SfoI SmlI SspI StyI TaqII TatI XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflIII AgeI AhdI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglI BglII BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BspMI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI CspCI DraI DraIII EciI EcoO109I EcoRV FseI FspI FspAI KpnI MfeI MluI MmeI MscI NaeI NcoI NgoMIV NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI PspOMI PspXI PsrI PstI PvuI RsrII SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I XbaI XhoI XmaI