################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MaeII MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Alw44I 875 GTGCAC G^TGCAC AocI 851 CCTCAGG CC^T[ACGT]AGG ApaI 968 GGGCCC GGGCC^C ApaLI 875 GTGCAC G^TGCAC AvaI 1043 CTCGAG C^[CT]CG[GA]G BalI 1530 TGGCCA TGG^CCA BamHI 1788 GGATCC G^GATCC BciVI 1857 GTATCC GTATCC(6/5) BsaWI 222 ACCGGA [AT]^CCGG[AT] BseRI 337 GAGGAG GAGGAG(10/8) BsgI 1513 CTGCAC GTGCAG(16/14) BsoBI 1043 CTCGAG C^[CT]CG[GA]G BstBI 2238 TTCGAA TT^CGAA BstXI 2096 CCAATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 851 CCTCAGG CC^T[ACGT]AGG CfoI 135 GCGC GCG^C DraI 802 TTTAAA TTT^AAA DsaI 564 CCACGG C^C[GA][CT]GG EaeI 1530 TGGCCA [CT]^GGCC[GA] EarI 2007 GAAGAG CTCTTC(1/4) Eco81I 851 CCTCAGG CC^T[ACGT]AGG EcoNI 285 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2318 ATGCAT ATGCA^T FauI 971 CCCGC CCCGC(4/6) HapII 223 CCGG C^CGG HgaI 522 GCGTC GACGC(5/10) HhaI 135 GCGC GCG^C HinP1I 135 GCGC G^CGC HincII 2171 GTCAAC GT[CT]^[GA]AC HindII 2171 GTCAAC GT[CT]^[GA]AC HindIII 8 AAGCTT A^AGCTT HpaII 223 CCGG C^CGG Ksp632I 2007 GAAGAG CTCTTC(1/4) MluNI 1530 TGGCCA TGG^CCA MscI 1530 TGGCCA TGG^CCA MslI 952 CACCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 223 CCGG C^CGG NdeI 1993 CATATG CA^TATG NsiI 2318 ATGCAT ATGCA^T NspV 2238 TTCGAA TT^CGAA PaeR7I 1043 CTCGAG C^TCGAG PflMI 639 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 634 AGGTCCC [GA]G^G[AT]CC[CT] SacI 295 GAGCTC GAGCT^C ScaI 895 AGTACT AGT^ACT SexAI 1460 ACCTGGT A^CC[AT]GGT SfuI 2238 TTCGAA TT^CGAA SphI 974 GCATGC GCATG^C Van91I 639 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1043 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 129 CCGC CCGC(-3/-1) 972 CCGC AcsI 1593 AAATTT [GA]^AATT[CT] 1706 AAATTT ApoI 1593 AAATTT [GA]^AATT[CT] 1706 AAATTT BanII 295 GAGCTC G[GA]GC[CT]^C 968 GGGCCC BbsI 312 GAAGAC GAAGAC(2/6) 651 GAAGAC BpuAI 312 GAAGAC GAAGAC(2/6) 651 GAAGAC BsaBI 1783 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1789 GATCCTGATC BsaI 219 GAGACC GGTCTC(1/5) 1566 GAGACC BsmI 1651 GAATGC GAATGC(1/-1) 2200 GAATGC BspMI 1511 ACCTGC ACCTGC(4/8) 81 GCAGGT DraII 18 AGGCCCT [GA]G^G[ACGT]CC[CT] 634 AGGTCCC EcoO109I 18 AGGCCCT [GA]G^G[ACGT]CC[CT] 634 AGGTCCC MamI 1783 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1789 GATCCTGATC NspI 422 ACATGC [GA]CATG^[CT] 974 GCATGC PstI 683 CTGCAG CTGCA^G 1001 CTGCAG StuI 395 AGGCCT AGG^CCT 1065 AGGCCT TaqI 1044 TCGA T^CGA 2239 TCGA TthHB8I 1044 TCGA T^CGA 2239 TCGA XbaI 363 TCTAGA T^CTAGA 2358 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AfaI AluI AlwI AlwNI AspHI AvaII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII