(Circular) (Six-base) MAPSORT of: pFN21AB0406.seq Check: 9515 from: 1 to: 7132 pFN21AB0406 7132 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2077 2077 Size: 7132 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4942 278 Size: 53 83 186 936 3406 2468 Fragments arranged by size: 3406 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4475 1937 Size: 2538 4594 Acc65I G'GTAC_C Cuts at: 4453 4453 Size: 7132 AclI AA'CG_TT Cuts at: 1563 5259 5632 1563 Size: 3696 373 3063 Fragments arranged by size: 3696 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2528 2799 2850 2966 5195 6579 6847 1632 Size: 896 271 51 116 2229 1384 268 1917 Fragments arranged by size: 2229 1917 1384 896 271 268 116 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6935 AflIII A'CryG_T Cuts at: 1203 6031 7008 1203 Size: 4828 977 1327 Fragments arranged by size: 4828 1327 977 AgeI A'CCGG_T Cuts at: 5963 5963 Size: 7132 AhdI GACnn_n'nnGTC Cuts at: 5861 5861 Size: 7132 AleI CACnn'nnGTG Cuts at: 1166 2961 1166 Size: 1795 5337 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7132 AlwNI CAG_nnn'CTG Cuts at: 2027 2281 2488 3466 4344 4875 6447 2027 Size: 254 207 978 878 531 1572 2712 Fragments arranged by size: 2712 1572 978 878 531 254 207 ApaI G_GGCC'C Cuts at: 2977 2977 Size: 7132 ApaLI G'TGCA_C Cuts at: 2880 5189 6345 2880 Size: 2309 1156 3667 Fragments arranged by size: 3667 2309 1156 ApoI r'AATT_y Cuts at: 1493 3598 3711 4441 4667 4819 6928 1493 Size: 2105 113 730 226 152 2109 1697 Fragments arranged by size: 2109 2105 1697 730 226 152 113 AseI AT'TA_AT Cuts at: 160 1032 5686 160 Size: 872 4654 1606 Fragments arranged by size: 4654 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7132 AvaI C'yCGr_G Cuts at: 1870 1943 3048 4457 1870 Size: 73 1105 1409 4545 Fragments arranged by size: 4545 1409 1105 73 BamHI G'GATC_C Cuts at: 3793 4462 4829 3793 Size: 669 367 6096 Fragments arranged by size: 6096 669 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4453 5908 618 Size: 325 217 627 2666 1455 1842 Fragments arranged by size: 2666 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2304 2977 4451 1391 Size: 913 673 1474 4072 Fragments arranged by size: 4072 1474 913 673 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2324 2663 928 Size: 971 425 339 5397 Fragments arranged by size: 5397 971 425 339 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5323 5357 1903 Size: 34 3386 34 3678 Fragments arranged by size: 3678 3386 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3873 5024 6234 3873 Size: 1151 1210 4771 Fragments arranged by size: 4771 1210 1151 BclI T'GATC_A Cuts at: 1548 1548 Size: 7132 BfrBI ATG'CAT Cuts at: 4325 4325 Size: 7132 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5743 136 Size: 107 122 71 631 605 4071 1525 Fragments arranged by size: 4071 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7127 7127 Size: 7132 BlpI GC'TnA_GC Cuts at: 4549 4549 Size: 7132 Bme1580I G_kGCm'C Cuts at: 1790 2884 2977 5193 6349 1790 Size: 1094 93 2216 1156 2573 Fragments arranged by size: 2573 2216 1156 1094 93 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7132 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2627 3254 4863 Size: 416 53 83 399 24 1210 627 1609 Cuts at: 4863 5821 442 Size: 958 1753 Fragments arranged by size: 1753 1609 1210 958 627 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7132 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2128 2733 2128 Size: 605 6527 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 2291 2351 2381 2465 2570 5792 1239 Size: 1049 3 60 30 84 105 3222 2579 Fragments arranged by size: 3222 2579 1049 105 84 60 30 3 Bpu10I CC'TnA_GC Cuts at: 2745 2745 Size: 7132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2517 3224 5257 6122 6420 2517 Size: 707 2033 865 298 3229 Fragments arranged by size: 3229 2033 865 707 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2218 3565 5795 882 Size: 617 719 1347 2230 2219 Fragments arranged by size: 2230 2219 1347 719 617 BsaAI yAC'GTr Cuts at: 493 1204 4923 493 Size: 711 3719 2702 Fragments arranged by size: 3719 2702 711 BsaBI GATnn'nnATC Cuts at: 3792 3798 7126 3792 Size: 6 3328 3798 Fragments arranged by size: 3798 3328 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4939 5321 5956 275 Size: 53 83 186 936 3406 382 635 1451 Fragments arranged by size: 3406 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2227 5565 5963 6237 6384 6795 2227 Size: 3338 398 274 147 411 2564 Fragments arranged by size: 3338 2564 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2075 2105 1178 Size: 30 165 30 672 30 6205 Fragments arranged by size: 6205 672 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2357 1186 Size: 1171 5961 BseYI C'CCAG_C Cuts at: 6335 6831 6335 Size: 496 6636 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3503 1163 Size: 573 1767 4792 Fragments arranged by size: 4792 1767 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4582 5343 6371 1540 Size: 275 186 2581 761 1028 2301 Fragments arranged by size: 2581 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 2884 4100 5193 5278 6349 2304 Size: 580 1216 1093 85 1071 3087 Fragments arranged by size: 3087 1216 1093 1071 580 85 BsmI GAATG_Cn' Cuts at: 3662 4211 4643 4736 3662 Size: 549 432 93 6058 Fragments arranged by size: 6058 549 432 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2884 2977 4100 4451 5193 5278 Size: 399 514 580 93 1123 351 742 85 Cuts at: 5278 6349 1391 Size: 1071 2174 Fragments arranged by size: 2174 1123 1071 742 580 514 399 351 93 85 BspEI T'CCGG_A Cuts at: 6795 6795 Size: 7132 BspHI T'CATG_A Cuts at: 5021 6751 5021 Size: 1730 5402 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2077 3525 4487 844 Size: 1233 1448 962 3489 Fragments arranged by size: 3489 1448 1233 962 BsrBI CCG'CTC Cuts at: 1394 1823 5019 1394 Size: 429 3196 3507 Fragments arranged by size: 3507 3196 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5627 5801 65 Size: 1183 4379 174 1396 Fragments arranged by size: 4379 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5776 5963 1700 Size: 110 3966 187 2869 Fragments arranged by size: 3966 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7132 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7132 BssSI C'ACGA_G Cuts at: 5192 6204 5192 Size: 1012 6120 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7132 BstBI TT'CG_AA Cuts at: 4244 5936 4244 Size: 1692 5440 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7132 BstXI CCAn_nnnn'nTGG Cuts at: 4108 4108 Size: 7132 BstYI r'GATC_y Cuts at: 1972 2071 3120 3793 4462 4829 5214 5231 6683 Size: 99 1049 673 669 367 385 17 1452 Cuts at: 6683 6769 6781 7127 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1049 673 669 385 367 346 99 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2857 2857 Size: 7132 BtgI C'CryG_G Cuts at: 513 1065 2569 513 Size: 552 1504 5076 Fragments arranged by size: 5076 1504 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6393 Fragments arranged by size: 6393 683 56 BtsI GCAGTG_nn' Cuts at: 2355 4648 5442 5462 2355 Size: 2293 794 20 4025 Fragments arranged by size: 4025 2293 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7132 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7097 DraI TTT'AAA Cuts at: 2809 4436 2809 Size: 1627 5505 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6139 809 Size: 507 4823 1802 Fragments arranged by size: 4823 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3535 4579 5468 Size: 54 1425 50 275 18 1705 1044 889 Cuts at: 5468 7097 8 Size: 1629 43 Fragments arranged by size: 1705 1629 1425 1044 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4579 1537 Size: 275 2767 4090 Fragments arranged by size: 4090 2767 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4007 5062 1353 Size: 2654 1055 3423 Fragments arranged by size: 3423 2654 1055 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5719 6093 6239 387 Size: 1064 138 4130 374 146 1280 Fragments arranged by size: 4130 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2302 2302 Size: 7132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2291 2351 2381 2465 2528 2570 Size: 393 656 3 60 30 84 63 42 Cuts at: 2570 2799 2850 2966 5195 5792 6579 6847 1239 Size: 229 51 116 2229 597 787 268 1524 Fragments arranged by size: 2229 1524 787 656 597 393 268 229 116 84 63 60 51 42 30 3 EcoNI CCTnn'n_nnAGG Cuts at: 2294 2294 Size: 7132 EcoO109I rG'GnC_Cy Cuts at: 2024 2640 2024 Size: 616 6516 EcoRI G'AATT_C Cuts at: 4441 4819 4441 Size: 378 6754 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2066 2066 Size: 7132 FspI TGC'GCA Cuts at: 5638 5638 Size: 7132 HaeII r_GCGC'y Cuts at: 1678 6279 1678 Size: 4601 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2311 2343 2684 2716 2752 Size: 32 135 32 739 32 341 32 36 Cuts at: 2752 2784 3465 3497 5773 5805 5847 5879 1373 Size: 32 681 32 2276 32 42 32 2626 Fragments arranged by size: 2626 2276 739 681 341 135 42 36 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4178 4476 4722 669 Size: 988 281 2240 298 246 3079 Fragments arranged by size: 3079 2240 988 298 281 246 HindIII A'AGCT_T Cuts at: 748 2013 748 Size: 1265 5867 HpaI GTT'AAC Cuts at: 4722 4722 Size: 7132 KpnI G_GTAC'C Cuts at: 4457 4457 Size: 7132 MfeI C'AATT_G Cuts at: 4731 4731 Size: 7132 MluI A'CGCG_T Cuts at: 7008 7008 Size: 7132 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3152 6246 6430 1305 Size: 1847 3094 184 2007 Fragments arranged by size: 3094 2007 1847 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3537 10 Size: 54 1425 343 1705 3605 Fragments arranged by size: 3605 1705 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2961 5090 5449 5608 518 Size: 648 1795 2129 359 159 2042 Fragments arranged by size: 2129 2042 1795 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4548 5225 6373 6618 1606 Size: 2942 677 1148 245 2120 Fragments arranged by size: 2942 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6580 NdeI CA'TA_TG Cuts at: 387 3999 387 Size: 3612 3520 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7132 NotI GC'GGCC_GC Cuts at: 4579 4579 Size: 7132 NsiI A_TGCA'T Cuts at: 4327 4327 Size: 7132 NspI r_CATG'y Cuts at: 1132 1229 2431 2983 4490 6035 1132 Size: 97 1202 552 1507 1545 2229 Fragments arranged by size: 2229 1545 1507 1202 552 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7132 PciI A'CATG_T Cuts at: 6031 6031 Size: 7132 PflMI CCAn_nnn'nTGG Cuts at: 1108 2650 1108 Size: 1542 5590 PfoI T'CCnGG_A Cuts at: 2586 2586 Size: 7132 PmeI GTTT'AAAC Cuts at: 4436 4436 Size: 7132 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5201 5233 6714 6746 5201 Size: 32 1481 32 5587 Fragments arranged by size: 5587 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2640 2640 Size: 7132 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7132 PsiI TTA'TAA Cuts at: 4702 4702 Size: 7132 PspOMI G'GGCC_C Cuts at: 2973 2973 Size: 7132 PspXI vC'TCGA_Gb Cuts at: 3048 3048 Size: 7132 PstI C_TGCA'G Cuts at: 830 2692 3010 4484 830 Size: 1862 318 1474 3478 Fragments arranged by size: 3478 1862 1474 318 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7132 SacI G_AGCT'C Cuts at: 2304 2304 Size: 7132 SalI G'TCGA_C Cuts at: 1936 4474 1936 Size: 2538 4594 SbfI CC_TGCA'GG Cuts at: 4484 4484 Size: 7132 ScaI AGT'ACT Cuts at: 2902 5380 7092 2902 Size: 2478 1712 2942 Fragments arranged by size: 2942 2478 1712 SexAI A'CCwGG_T Cuts at: 3465 3465 Size: 7132 SfcI C'TryA_G Cuts at: 826 1045 2613 2688 3006 4480 5615 6296 6487 Size: 219 1568 75 318 1474 1135 681 191 Cuts at: 6487 826 Size: 1471 Fragments arranged by size: 1568 1474 1471 1135 681 318 219 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7132 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7132 SmaI CCC'GGG Cuts at: 1872 4459 1872 Size: 2587 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2532 3048 3239 5236 6137 6399 Size: 197 926 589 516 191 1997 901 262 Cuts at: 6399 820 Size: 1553 Fragments arranged by size: 1997 1553 926 901 589 516 262 197 191 SnaBI TAC'GTA Cuts at: 493 4923 493 Size: 4430 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7132 SphI G_CATG'C Cuts at: 1132 2983 4490 1132 Size: 1851 1507 3774 Fragments arranged by size: 3774 1851 1507 SspI AAT'ATT Cuts at: 5 52 5056 5 Size: 47 5004 2081 Fragments arranged by size: 5004 2081 47 StuI AGG'CCT Cuts at: 1517 2402 3072 1517 Size: 885 670 5577 Fragments arranged by size: 5577 885 670 StyI C'CwwG_G Cuts at: 513 1065 2241 2295 3067 4371 4571 513 Size: 552 1176 54 772 1304 200 3074 Fragments arranged by size: 3074 1304 1176 772 552 200 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2448 2973 3983 5159 5176 5329 5514 Size: 1255 868 525 1010 1176 17 153 185 Cuts at: 5514 5959 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1176 1010 868 525 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2900 5378 7090 Size: 274 80 33 51 1443 923 2478 1712 Cuts at: 7090 96 Size: 138 Fragments arranged by size: 2478 1712 1443 923 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2075 2107 2716 2748 2975 3007 3362 3394 3807 Size: 32 609 32 227 32 355 32 413 Cuts at: 3807 3839 7092 7124 2075 Size: 32 3253 32 2083 Fragments arranged by size: 3253 2083 609 413 355 227 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7132 XbaI T'CTAG_A Cuts at: 2368 4363 4468 2368 Size: 1995 105 5032 Fragments arranged by size: 5032 1995 105 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2489 2552 2967 3168 3680 2489 Size: 63 415 201 512 5941 Fragments arranged by size: 5941 512 415 201 63 XhoI C'TCGA_G Cuts at: 1943 3048 1943 Size: 1105 6027 XmaI C'CCGG_G Cuts at: 1870 4457 1870 Size: 2587 4545 XmnI GAAnn'nnTTC Cuts at: 5261 5261 Size: 7132 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4940 276 Size: 53 83 186 936 3406 2468 Fragments arranged by size: 3406 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI