(Linear) (Six-base) MAPSORT of: KIBB0406.seq Check: 786 from: 1 to: 2427 KIBB0406.orf With 182 enzymes: SgfI * July 3, 2012 16:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 72 2427 Size: 72 2355 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 794 845 961 2427 Size: 523 271 51 116 1466 Fragments arranged by size: 1466 523 271 116 51 AleI CACnn'nnGTG Cuts at: 0 956 2427 Size: 956 1471 AlwNI CAG_nnn'CTG Cuts at: 0 22 276 483 1461 2339 2427 Size: 22 254 207 978 878 88 Fragments arranged by size: 978 878 254 207 88 22 ApaI G_GGCC'C Cuts at: 0 972 2427 Size: 972 1455 ApaLI G'TGCA_C Cuts at: 0 875 2427 Size: 875 1552 ApoI r'AATT_y Cuts at: 0 1593 1706 2427 Size: 1593 113 721 Fragments arranged by size: 1593 721 113 AvaI C'yCGr_G Cuts at: 0 1043 2427 Size: 1043 1384 BamHI G'GATC_C Cuts at: 0 1788 2427 Size: 1788 639 BanII G_rGCy'C Cuts at: 0 299 972 2427 Size: 299 673 1455 Fragments arranged by size: 1455 673 299 BbsI GAAGACnn'nnnn_ Cuts at: 0 319 658 2427 Size: 319 339 1769 Fragments arranged by size: 1769 339 319 BciVI GTATCCnnnnn_n' Cuts at: 0 1868 2427 Size: 1868 559 BfrBI ATG'CAT Cuts at: 0 2320 2427 Size: 2320 107 Bme1580I G_kGCm'C Cuts at: 0 879 972 2427 Size: 879 93 1455 Fragments arranged by size: 1455 879 93 BmrI ACTGGGnnnn_n' Cuts at: 0 622 1249 2427 Size: 622 627 1178 Fragments arranged by size: 1178 627 622 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 123 728 2427 Size: 123 605 1699 Fragments arranged by size: 1699 605 123 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 286 346 376 460 565 2427 Size: 283 3 60 30 84 105 1862 Fragments arranged by size: 1862 283 105 84 60 30 3 Bpu10I CC'TnA_GC Cuts at: 0 740 2427 Size: 740 1687 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 512 1219 2427 Size: 512 707 1208 Fragments arranged by size: 1208 707 512 BsaI GGTCTCn'nnnn_ Cuts at: 0 213 1560 2427 Size: 213 1347 867 Fragments arranged by size: 1347 867 213 BsaBI GATnn'nnATC Cuts at: 0 1787 1793 2427 Size: 1787 6 634 Fragments arranged by size: 1787 634 6 BsaWI w'CCGG_w Cuts at: 0 222 2427 Size: 222 2205 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 70 100 2427 Size: 70 30 2327 Fragments arranged by size: 2327 70 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 352 2427 Size: 352 2075 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1498 2427 Size: 1498 929 BsiHKAI G_wGCw'C Cuts at: 0 299 879 2095 2427 Size: 299 580 1216 332 Fragments arranged by size: 1216 580 332 299 BsmI GAATG_Cn' Cuts at: 0 1657 2206 2427 Size: 1657 549 221 Fragments arranged by size: 1657 549 221 Bsp1286I G_dGCh'C Cuts at: 0 299 879 972 2095 2427 Size: 299 580 93 1123 332 Fragments arranged by size: 1123 580 332 299 93 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 72 1520 2427 Size: 72 1448 907 Fragments arranged by size: 1448 907 72 BstBI TT'CG_AA Cuts at: 0 2239 2427 Size: 2239 188 BstXI CCAn_nnnn'nTGG Cuts at: 0 2103 2427 Size: 2103 324 BstYI r'GATC_y Cuts at: 0 66 1115 1788 2427 Size: 66 1049 673 639 Fragments arranged by size: 1049 673 639 66 Bsu36I CC'TnA_GG Cuts at: 0 852 2427 Size: 852 1575 BtgI C'CryG_G Cuts at: 0 564 2427 Size: 564 1863 BtsI GCAGTG_nn' Cuts at: 0 350 2427 Size: 350 2077 DraI TTT'AAA Cuts at: 0 804 2427 Size: 804 1623 EaeI y'GGCC_r Cuts at: 0 1530 2427 Size: 1530 897 EarI CTCTTCn'nnn_ Cuts at: 0 2002 2427 Size: 2002 425 EcoICRI GAG'CTC Cuts at: 0 297 2427 Size: 297 2130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 286 346 376 460 523 565 794 Size: 283 3 60 30 84 63 42 229 Cuts at: 794 845 961 2427 Size: 51 116 1466 Fragments arranged by size: 1466 283 229 116 84 63 60 51 42 30 3 EcoNI CCTnn'n_nnAGG Cuts at: 0 289 2427 Size: 289 2138 EcoO109I rG'GnC_Cy Cuts at: 0 19 635 2427 Size: 19 616 1792 Fragments arranged by size: 1792 616 19 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 61 2427 Size: 61 2366 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 306 338 679 711 747 779 1460 1492 Size: 306 32 341 32 36 32 681 32 Cuts at: 1492 2427 Size: 935 Fragments arranged by size: 935 681 341 306 36 32 32 32 32 HincII GTy'rAC Cuts at: 0 2173 2427 Size: 2173 254 HindIII A'AGCT_T Cuts at: 0 8 2427 Size: 8 2419 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1147 2427 Size: 1147 1280 MscI TGG'CCA Cuts at: 0 1532 2427 Size: 1532 895 MslI CAynn'nnrTG Cuts at: 0 956 2427 Size: 956 1471 NdeI CA'TA_TG Cuts at: 0 1994 2427 Size: 1994 433 NsiI A_TGCA'T Cuts at: 0 2322 2427 Size: 2322 105 NspI r_CATG'y Cuts at: 0 426 978 2427 Size: 426 552 1449 Fragments arranged by size: 1449 552 426 PflMI CCAn_nnn'nTGG Cuts at: 0 645 2427 Size: 645 1782 PfoI T'CCnGG_A Cuts at: 0 581 2427 Size: 581 1846 PpuMI rG'GwC_Cy Cuts at: 0 635 2427 Size: 635 1792 PspOMI G'GGCC_C Cuts at: 0 968 2427 Size: 968 1459 PspXI vC'TCGA_Gb Cuts at: 0 1043 2427 Size: 1043 1384 PstI C_TGCA'G Cuts at: 0 687 1005 2427 Size: 687 318 1422 Fragments arranged by size: 1422 687 318 SacI G_AGCT'C Cuts at: 0 299 2427 Size: 299 2128 ScaI AGT'ACT Cuts at: 0 897 2427 Size: 897 1530 SexAI A'CCwGG_T Cuts at: 0 1460 2427 Size: 1460 967 SfcI C'TryA_G Cuts at: 0 608 683 1001 2427 Size: 608 75 318 1426 Fragments arranged by size: 1426 608 318 75 SmlI C'TyrA_G Cuts at: 0 527 1043 1234 2427 Size: 527 516 191 1193 Fragments arranged by size: 1193 527 516 191 SphI G_CATG'C Cuts at: 0 978 2427 Size: 978 1449 StuI AGG'CCT Cuts at: 0 397 1067 2427 Size: 397 670 1360 Fragments arranged by size: 1360 670 397 StyI C'CwwG_G Cuts at: 0 236 290 1062 2366 2427 Size: 236 54 772 1304 61 Fragments arranged by size: 1304 772 236 61 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 443 968 1978 2427 Size: 443 525 1010 449 Fragments arranged by size: 1010 525 449 443 TatI w'GTAC_w Cuts at: 0 895 2427 Size: 895 1532 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 70 102 711 743 970 1002 1357 1389 Size: 70 32 609 32 227 32 355 32 Cuts at: 1389 1802 1834 2427 Size: 413 32 593 Fragments arranged by size: 609 593 413 355 227 70 32 32 32 32 32 XbaI T'CTAG_A Cuts at: 0 363 2358 2427 Size: 363 1995 69 Fragments arranged by size: 1995 363 69 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 484 547 962 1163 1675 2427 Size: 484 63 415 201 512 752 Fragments arranged by size: 752 512 484 415 201 63 XhoI C'TCGA_G Cuts at: 0 1043 2427 Size: 1043 1384 Enzymes that do cut: AarI AcuI AleI AlwNI ApaI ApaLI ApoI AvaI BamHI BanII BbsI BciVI BfrBI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BstBI BstXI BstYI Bsu36I BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII MmeI MscI MslI NdeI NsiI NspI PflMI PfoI PpuMI PspOMI PspXI PstI SacI ScaI SexAI SfcI SmlI SphI StuI StyI TaqII TatI TstI XbaI XcmI XhoI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BclI BglI BglII BlpI BmgBI BmtI BsaAI BsaHI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstZ17I BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XmaI XmnI ZraI