################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII Aor51HI AscI AseI AsnI Asp700I Asp718I AvrII BbeI BclI BfaI BfrI BlnI BsaBI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssSI Bst1107I BstZ17I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I FbaI FseI HindIII HpaI KasI KpnI MaeI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI PacI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 393 GAATTC [GA]^AATT[CT] AgeI 2395 ACCGGT A^CCGGT AhdI 1513 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 393 GAATTC [GA]^AATT[CT] AspEI 1513 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 901 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2436 TGCGCA TGC^GCA BbrPI 1819 CACGTG CAC^GTG BbsI 1869 GAAGAC GAAGAC(2/6) BcgI 1295 CGAGGGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 154 GTATCC GTATCC(6/5) BlpI 2937 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2937 GCTCAGC GC^T[ACGT]AGC BpuAI 1869 GAAGAC GAAGAC(2/6) BsaAI 1819 CACGTG [CT]AC^GT[GA] BsaI 1611 GGTCTC GGTCTC(1/5) BsrDI 1602 CATTGC GCAATG(2/0) BssHII 2528 GCGCGC G^CGCGC BstAPI 2059 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 956 TTCGAA TT^CGAA CelII 2937 GCTCAGC GC^T[ACGT]AGC Eam1105I 1513 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 393 GAATTC G^AATTC EcoT22I 1859 ATGCAT ATGCA^T FspI 2436 TGCGCA TGC^GCA HaeII 758 AGCGCC [GA]GCGC^[CT] HincII 1849 GTCAAC GT[CT]^[GA]AC HindII 1849 GTCAAC GT[CT]^[GA]AC KspI 221 CCGCGG CCGC^GG NsiI 1859 ATGCAT ATGCA^T NspI 1328 GCATGC [GA]CATG^[CT] NspV 956 TTCGAA TT^CGAA PflFI 901 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1566 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2395 ACCGGT A^CCGGT PmaCI 1819 CACGTG CAC^GTG PmlI 1819 CACGTG CAC^GTG PshAI 318 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 221 CCGCGG CCGC^GG SapI 229 GCTCTTC GCTCTTC(1/4) SexAI 902 ACCAGGT A^CC[AT]GGT SfuI 956 TTCGAA TT^CGAA SmaI 320 CCCGGG CCC^GGG SphI 1328 GCATGC GCATG^C StuI 2561 AGGCCT AGG^CCT Tth111I 901 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1566 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 320 CCCGGG C^CCGGG XmaI 320 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 1814 GACGCC G[GA]^CG[CT]C 2713 GGCGTC Alw44I 1657 GTGCAC G^TGCAC 2703 GTGCAC AocI 1413 CCTGAGG CC^T[ACGT]AGG 2564 CCTCAGG ApaI 2354 GGGCCC GGGCC^C 2897 GGGCCC ApaLI 1657 GTGCAC G^TGCAC 2703 GTGCAC BalI 302 TGGCCA TGG^CCA 2210 TGGCCA BamHI 1157 GGATCC G^GATCC 2875 GGATCC BanI 1406 GGCACC G^G[CT][GA]CC 1664 GGCACC BglII 517 AGATCT A^GATCT 2625 AGATCT BsaHI 1814 GACGCC G[GA]^CG[CT]C 2713 GGCGTC BsgI 2497 CTGCAC GTGCAG(16/14) 1318 GTGCAG BsmBI 62 GAGACG CGTCTC(1/5) 1447 GAGACG BstEII 192 GGTCACC G^GT[ACGT]ACC 324 GGTCACC BstPI 192 GGTCACC G^GT[ACGT]ACC 324 GGTCACC Bsu36I 1413 CCTGAGG CC^T[ACGT]AGG 2564 CCTCAGG DrdI 1108 GACGTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1930 GACGATATTGTC Eco81I 1413 CCTGAGG CC^T[ACGT]AGG 2564 CCTCAGG EcoNI 1164 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1275 CCTGGCTGAGG EcoO65I 192 GGTCACC G^GT[ACGT]ACC 324 GGTCACC EcoRV 259 GATATC GAT^ATC 883 GATATC Hin1I 1814 GACGCC G[GA]^CG[CT]C 2713 GGCGTC MluNI 302 TGGCCA TGG^CCA 2210 TGGCCA MscI 302 TGGCCA TGG^CCA 2210 TGGCCA NcoI 636 CCATGG C^CATGG 2787 CCATGG TfiI 783 GATTC G^A[AT]TC 2828 GAATC XcmI 636 CCATGGACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 731 CCAACATCGCCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanII BbvCI BbvI BcnI BglI BmyI BpmI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PaeR7I PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoI XhoII