(Linear) (Six-base) MAPSORT of: KIBB0472.seq Check: 161 from: 1 to: 2979 KIBB0472.orf With 182 enzymes: SgfI * July 3, 2012 16:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 577 2073 2695 2783 2979 Size: 577 1496 622 88 196 Fragments arranged by size: 1496 622 577 196 88 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 1009 1102 1189 2132 2979 Size: 163 846 93 87 943 847 Fragments arranged by size: 943 847 846 163 93 87 AgeI A'CCGG_T Cuts at: 0 2395 2979 Size: 2395 584 AhdI GACnn_n'nnGTC Cuts at: 0 1518 2979 Size: 1518 1461 AleI CACnn'nnGTG Cuts at: 0 667 2979 Size: 667 2312 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2239 2979 Size: 2239 740 AlwNI CAG_nnn'CTG Cuts at: 0 89 1572 2065 2979 Size: 89 1483 493 914 Fragments arranged by size: 1483 914 493 89 ApaI G_GGCC'C Cuts at: 0 2358 2901 2979 Size: 2358 543 78 Fragments arranged by size: 2358 543 78 ApaLI G'TGCA_C Cuts at: 0 1657 2703 2979 Size: 1657 1046 276 Fragments arranged by size: 1657 1046 276 ApoI r'AATT_y Cuts at: 0 393 2979 Size: 393 2586 AvaI C'yCGr_G Cuts at: 0 55 320 1015 1093 1141 2366 2779 2979 Size: 55 265 695 78 48 1225 413 200 Fragments arranged by size: 1225 695 413 265 200 78 55 48 BamHI G'GATC_C Cuts at: 0 1157 2875 2979 Size: 1157 1718 104 Fragments arranged by size: 1718 1157 104 BanI G'GyrC_C Cuts at: 0 1406 1664 2979 Size: 1406 258 1315 Fragments arranged by size: 1406 1315 258 BanII G_rGCy'C Cuts at: 0 56 119 206 2358 2808 2901 2939 2979 Size: 56 63 87 2152 450 93 38 40 Fragments arranged by size: 2152 450 93 87 63 56 40 38 BbsI GAAGACnn'nnnn_ Cuts at: 0 1876 2979 Size: 1876 1103 BbvCI CC'TCA_GC Cuts at: 0 1280 1775 2299 2764 2979 Size: 1280 495 524 465 215 Fragments arranged by size: 1280 524 495 465 215 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1284 1318 2979 Size: 1284 34 1661 Fragments arranged by size: 1661 1284 34 BciVI GTATCCnnnnn_n' Cuts at: 0 165 2979 Size: 165 2814 BfrBI ATG'CAT Cuts at: 0 1861 2979 Size: 1861 1118 BglI GCCn_nnn'nGGC Cuts at: 0 1144 2171 2674 2896 2979 Size: 1144 1027 503 222 83 Fragments arranged by size: 1144 1027 503 222 83 BglII A'GATC_T Cuts at: 0 517 2625 2979 Size: 517 2108 354 Fragments arranged by size: 2108 517 354 BlpI GC'TnA_GC Cuts at: 0 2938 2979 Size: 2938 41 Bme1580I G_kGCm'C Cuts at: 0 870 1134 1460 1661 1812 2358 2426 2707 Size: 870 264 326 201 151 546 68 281 Cuts at: 2707 2901 2979 Size: 194 78 Fragments arranged by size: 870 546 326 281 264 201 194 151 78 68 BmgBI CAC'GTC Cuts at: 0 1110 2979 Size: 1110 1869 BmrI ACTGGGnnnn_n' Cuts at: 0 179 230 317 521 662 2979 Size: 179 51 87 204 141 2317 Fragments arranged by size: 2317 204 179 141 87 51 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 120 2979 Size: 120 2859 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 103 835 1522 1612 2398 2524 2979 Size: 70 33 732 687 90 786 126 455 Fragments arranged by size: 786 732 687 455 126 90 70 33 Bpu10I CC'TnA_GC Cuts at: 0 1280 1670 1775 2299 2764 2979 Size: 1280 390 105 524 465 215 Fragments arranged by size: 1280 524 465 390 215 105 BsaI GGTCTCn'nnnn_ Cuts at: 0 1617 2979 Size: 1617 1362 BsaAI yAC'GTr Cuts at: 0 1821 2979 Size: 1821 1158 BsaHI Gr'CG_yC Cuts at: 0 1815 2714 2979 Size: 1815 899 265 Fragments arranged by size: 1815 899 265 BsaWI w'CCGG_w Cuts at: 0 1781 2023 2395 2979 Size: 1781 242 372 584 Fragments arranged by size: 1781 584 372 242 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 70 100 849 879 2979 Size: 70 30 749 30 2100 Fragments arranged by size: 2100 749 70 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 91 480 1297 1519 1522 2302 2343 2653 Size: 91 389 817 222 3 780 41 310 Cuts at: 2653 2925 2979 Size: 272 54 Fragments arranged by size: 817 780 389 310 272 222 91 54 41 3 BseYI C'CCAG_C Cuts at: 0 483 1349 2092 2128 2385 2403 2886 2900 Size: 483 866 743 36 257 18 483 14 Cuts at: 2900 2979 Size: 79 Fragments arranged by size: 866 743 483 483 257 79 36 18 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1339 2482 2979 Size: 1339 1143 497 Fragments arranged by size: 1339 1143 497 BsiHKAI G_wGCw'C Cuts at: 0 56 119 509 1661 2294 2707 2939 2979 Size: 56 63 390 1152 633 413 232 40 Fragments arranged by size: 1152 633 413 390 232 63 56 40 BsmBI CGTCTCn'nnnn_ Cuts at: 0 56 1441 2979 Size: 56 1385 1538 Fragments arranged by size: 1538 1385 56 Bsp1286I G_dGCh'C Cuts at: 0 56 119 206 509 870 1134 1460 1661 Size: 56 63 87 303 361 264 326 201 Cuts at: 1661 1812 2294 2358 2426 2707 2808 2901 2939 Size: 151 482 64 68 281 101 93 38 Cuts at: 2939 2979 Size: 40 Fragments arranged by size: 482 361 326 303 281 264 201 151 101 93 87 68 64 63 56 40 38 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 53 74 141 577 1646 2013 2073 2695 Size: 53 21 67 436 1069 367 60 622 Cuts at: 2695 2783 2979 Size: 88 196 Fragments arranged by size: 1069 622 436 367 196 88 67 60 53 21 BsrDI GCAATG_nn' Cuts at: 0 1601 2979 Size: 1601 1378 BsrFI r'CCGG_y Cuts at: 0 1480 2088 2395 2710 2854 2979 Size: 1480 608 307 315 144 125 Fragments arranged by size: 1480 608 315 307 144 125 BssHII G'CGCG_C Cuts at: 0 2528 2979 Size: 2528 451 BstAPI GCAn_nnn'nTGC Cuts at: 0 2065 2979 Size: 2065 914 BstBI TT'CG_AA Cuts at: 0 957 2979 Size: 957 2022 BstEII G'GTnAC_C Cuts at: 0 192 324 2979 Size: 192 132 2655 Fragments arranged by size: 2655 192 132 BstXI CCAn_nnnn'nTGG Cuts at: 0 39 169 669 2199 2979 Size: 39 130 500 1530 780 Fragments arranged by size: 1530 780 500 130 39 BstYI r'GATC_y Cuts at: 0 311 517 1157 1741 2625 2783 2875 2979 Size: 311 206 640 584 884 158 92 104 Fragments arranged by size: 884 640 584 311 206 158 104 92 Bsu36I CC'TnA_GG Cuts at: 0 1414 2565 2979 Size: 1414 1151 414 Fragments arranged by size: 1414 1151 414 BtgI C'CryG_G Cuts at: 0 221 636 740 1397 2633 2787 2979 Size: 221 415 104 657 1236 154 192 Fragments arranged by size: 1236 657 415 221 192 154 104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 720 2979 Size: 720 2259 DrdI GACnn_nn'nnGTC Cuts at: 0 1114 1936 2979 Size: 1114 822 1043 Fragments arranged by size: 1114 1043 822 EaeI y'GGCC_r Cuts at: 0 302 2210 2618 2979 Size: 302 1908 408 361 Fragments arranged by size: 1908 408 361 302 EarI CTCTTCn'nnn_ Cuts at: 0 236 1398 1439 2320 2979 Size: 236 1162 41 881 659 Fragments arranged by size: 1162 881 659 236 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1534 2979 Size: 1534 1445 EcoICRI GAG'CTC Cuts at: 0 54 117 2937 2979 Size: 54 63 2820 42 Fragments arranged by size: 2820 63 54 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 103 163 835 1009 1102 1189 1522 Size: 70 33 60 672 174 93 87 333 Cuts at: 1522 1612 2132 2398 2524 2979 Size: 90 520 266 126 455 Fragments arranged by size: 672 520 455 333 266 174 126 93 90 87 70 60 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 1168 1279 2979 Size: 1168 111 1700 Fragments arranged by size: 1700 1168 111 EcoO109I rG'GnC_Cy Cuts at: 0 86 1137 1419 2002 2354 2355 2380 2652 Size: 86 1051 282 583 352 1 25 272 Cuts at: 2652 2844 2897 2979 Size: 192 53 82 Fragments arranged by size: 1051 583 352 282 272 192 86 82 53 25 1 EcoRI G'AATT_C Cuts at: 0 393 2979 Size: 393 2586 EcoRV GAT'ATC Cuts at: 0 261 885 2979 Size: 261 624 2094 Fragments arranged by size: 2094 624 261 FspI TGC'GCA Cuts at: 0 2438 2979 Size: 2438 541 HaeII r_GCGC'y Cuts at: 0 762 2979 Size: 762 2217 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 70 102 1241 1273 1504 1536 2979 Size: 70 32 1139 32 231 32 1443 Fragments arranged by size: 1443 1139 231 70 32 32 32 HincII GTy'rAC Cuts at: 0 1851 2979 Size: 1851 1128 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 755 1769 2210 2979 Size: 755 1014 441 769 Fragments arranged by size: 1014 769 755 441 MscI TGG'CCA Cuts at: 0 304 2212 2979 Size: 304 1908 767 Fragments arranged by size: 1908 767 304 MslI CAynn'nnrTG Cuts at: 0 502 667 739 1606 2197 2341 2979 Size: 502 165 72 867 591 144 638 Fragments arranged by size: 867 638 591 502 165 144 72 MspA1I CmG'CkG Cuts at: 0 13 223 300 552 780 1209 2034 2861 Size: 13 210 77 252 228 429 825 827 Cuts at: 2861 2904 2979 Size: 43 75 Fragments arranged by size: 827 825 429 252 228 210 77 75 43 13 NaeI GCC'GGC Cuts at: 0 1482 2090 2712 2856 2979 Size: 1482 608 622 144 123 Fragments arranged by size: 1482 622 608 144 123 NcoI C'CATG_G Cuts at: 0 636 2787 2979 Size: 636 2151 192 Fragments arranged by size: 2151 636 192 NgoMIV G'CCGG_C Cuts at: 0 1480 2088 2710 2854 2979 Size: 1480 608 622 144 125 Fragments arranged by size: 1480 622 608 144 125 NsiI A_TGCA'T Cuts at: 0 1863 2979 Size: 1863 1116 NspI r_CATG'y Cuts at: 0 1332 2979 Size: 1332 1647 PasI CC'CwG_GG Cuts at: 0 1968 1998 2376 2456 2724 2979 Size: 1968 30 378 80 268 255 Fragments arranged by size: 1968 378 268 255 80 30 PflMI CCAn_nnn'nTGG Cuts at: 0 1572 2979 Size: 1572 1407 PfoI T'CCnGG_A Cuts at: 0 812 2979 Size: 812 2167 PmlI CAC'GTG Cuts at: 0 1821 2979 Size: 1821 1158 PpuMI rG'GwC_Cy Cuts at: 0 86 1419 2002 2380 2844 2979 Size: 86 1333 583 378 464 135 Fragments arranged by size: 1333 583 464 378 135 86 PshAI GACnn'nnGTC Cuts at: 0 322 2979 Size: 322 2657 PspOMI G'GGCC_C Cuts at: 0 2354 2897 2979 Size: 2354 543 82 Fragments arranged by size: 2354 543 82 PspXI vC'TCGA_Gb Cuts at: 0 1141 2979 Size: 1141 1838 PstI C_TGCA'G Cuts at: 0 152 2061 2516 2749 2909 2979 Size: 152 1909 455 233 160 70 Fragments arranged by size: 1909 455 233 160 152 70 PvuII CAG'CTG Cuts at: 0 13 300 1209 2904 2979 Size: 13 287 909 1695 75 Fragments arranged by size: 1695 909 287 75 13 SacI G_AGCT'C Cuts at: 0 56 119 2939 2979 Size: 56 63 2820 40 Fragments arranged by size: 2820 63 56 40 SacII CC_GC'GG Cuts at: 0 224 2979 Size: 224 2755 SanDI GG'GwC_CC Cuts at: 0 2380 2979 Size: 2380 599 SapI GCTCTTCn'nnn_ Cuts at: 0 236 2979 Size: 236 2743 SexAI A'CCwGG_T Cuts at: 0 902 2979 Size: 902 2077 SfcI C'TryA_G Cuts at: 0 148 399 2057 2512 2745 2905 2979 Size: 148 251 1658 455 233 160 74 Fragments arranged by size: 1658 455 251 233 160 148 74 SmaI CCC'GGG Cuts at: 0 322 2979 Size: 322 2657 SmlI C'TyrA_G Cuts at: 0 55 1015 1141 2979 Size: 55 960 126 1838 Fragments arranged by size: 1838 960 126 55 SphI G_CATG'C Cuts at: 0 1332 2979 Size: 1332 1647 StuI AGG'CCT Cuts at: 0 2563 2979 Size: 2563 416 StyI C'CwwG_G Cuts at: 0 636 1371 1701 2787 2979 Size: 636 735 330 1086 192 Fragments arranged by size: 1086 735 636 330 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 327 343 401 2979 Size: 327 16 58 2578 Fragments arranged by size: 2578 327 58 16 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 849 881 2979 Size: 849 32 2098 Fragments arranged by size: 2098 849 32 Tth111I GACn'n_nGTC Cuts at: 0 904 2979 Size: 904 2075 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 643 738 2979 Size: 643 95 2241 Fragments arranged by size: 2241 643 95 XhoI C'TCGA_G Cuts at: 0 55 1015 1141 2979 Size: 55 960 126 1838 Fragments arranged by size: 1838 960 126 55 XmaI C'CCGG_G Cuts at: 0 320 2979 Size: 320 2659 Enzymes that do cut: AarI AcuI AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SanDI SapI SexAI SfcI SmaI SmlI SphI StuI StyI TaqII TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AloI AscI AseI AsiSI AvrII BaeI BbeI BclI BmtI BpuEI BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssSI BstZ17I BtsI ClaI CspCI DraI DraIII EagI FalI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI PacI PciI PmeI PpiI PsiI PsrI PvuI RsrII SalI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI XbaI XmnI ZraI