################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BanII BbeI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AfaI 242 GTAC GT^AC AlwNI 124 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 430 ATTAAT AT^TAAT AsnI 430 ATTAAT AT^TAAT BalI 260 TGGCCA TGG^CCA BamHI 554 GGATCC G^GATCC BbrPI 563 CACGTG CAC^GTG BbsI 274 GAAGAC GAAGAC(2/6) BclI 183 TGATCA T^GATCA BfaI 67 CTAG C^TAG BpuAI 274 GAAGAC GAAGAC(2/6) BsaAI 563 CACGTG [CT]AC^GT[GA] BsaI 448 GGTCTC GGTCTC(1/5) BsaJI 203 CCGAGG C^C[ACGT][ACGT]GG BseRI 370 GAGGAG GAGGAG(10/8) BsmAI 449 GTCTC GTCTC(1/5) BsmFI 41 GGGAC GGGAC(10/14) BsmI 15 GCATTC GAATGC(1/-1) BspMI 282 GCAGGT ACCTGC(4/8) BsrBI 54 CCGCTC CCGCTC(-3/-3) BsrDI 407 GCAATG GCAATG(2/0) DdeI 270 CTTAG C^T[ACGT]AG DrdI 504 GACAGTAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 260 TGGCCA [CT]^GGCC[GA] FauI 404 CCCGC CCCGC(4/6) FbaI 183 TGATCA T^GATCA HindIII 233 AAGCTT A^AGCTT MaeI 67 CTAG C^TAG MaeII 564 ACGT A^CGT MaeIII 308 GTGAC ^GT[ACGT]AC MluNI 260 TGGCCA TGG^CCA MscI 260 TGGCCA TGG^CCA MspA1I 22 CCGCTG C[AC]G^C[GT]G NheI 66 GCTAGC G^CTAGC PflMI 559 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 563 CACGTG CAC^GTG PmlI 563 CACGTG CAC^GTG PpuMI 41 GGGACCT [GA]G^G[AT]CC[CT] PshBI 430 ATTAAT AT^TAAT PstI 484 CTGCAG CTGCA^G RsaI 242 GTAC GT^AC Tsp45I 308 GTGAC ^GT[GC]AC Van91I 559 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AvaII 42 GGACC G^G[AT]CC 512 GGTCC BbvI 349 GCTGC GCAGC(8/12) 483 GCTGC BsrI 550 ACTGG ACTGG(1/-1) 169 CCAGT BstYI 398 AGATCC [GA]^GATC[CT] 554 GGATCC Cac8I 66 GCTAGC GC[ACGT]^[ACGT]GC 175 GCAAGC MflI 398 AGATCC [GA]^GATC[CT] 554 GGATCC MseI 376 TTAA T^TAA 431 TTAA NlaIII 250 CATG CATG^ 520 CATG PleI 151 GACTC GAGTC(4/5) 215 GACTC SfcI 48 CTGTAG C^T[GA][CT]AG 484 CTGCAG TfiI 78 GATTC G^A[AT]TC 106 GAATC Tru9I 376 TTAA T^TAA 431 TTAA TseI 349 GCTGC G^C[AT]GC 483 GCTGC XhoII 398 AGATCC [GA]^GATC[CT] 554 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AciI AluI AlwI BsiYI BslI BssKI BstF5I BstNI Cfr13I DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI TspRI