Restriction map for
pFN21AE1690
(Circular) (Six-base) MAPSORT of: pFN21AE1690.seq Check: 6283 from: 1 to: 5305
pFN21AE1690 5305 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2278 2278
Size: 5305
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3115 278
Size: 53 83 186 936 1579 2468
Fragments arranged by size:
2468 1579 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2648 1937
Size: 711 4594
Acc65I G'GTAC_C
Cuts at: 2626 2626
Size: 5305
AclI AA'CG_TT
Cuts at: 1563 3432 3805 1563
Size: 1869 373 3063
Fragments arranged by size:
3063 1869 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3368 4752 5020 1632
Size: 1736 1384 268 1917
Fragments arranged by size:
1917 1736 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5108
AflIII A'CryG_T
Cuts at: 1203 4204 5181 1203
Size: 3001 977 1327
Fragments arranged by size:
3001 1327 977
AgeI A'CCGG_T
Cuts at: 4136 4136
Size: 5305
AhdI GACnn_n'nnGTC
Cuts at: 4034 4034
Size: 5305
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5305
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2071 2103 2071
Size: 32 5273
AlwNI CAG_nnn'CTG
Cuts at: 2134 3048 4620 2134
Size: 914 1572 2819
Fragments arranged by size:
2819 1572 914
ApaLI G'TGCA_C
Cuts at: 3362 4518 3362
Size: 1156 4149
ApoI r'AATT_y
Cuts at: 1493 2614 2840 2992 5101 1493
Size: 1121 226 152 2109 1697
Fragments arranged by size:
2109 1697 1121 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2436 3859 160
Size: 872 1404 1423 1606
Fragments arranged by size:
1606 1423 1404 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5305
AvaI C'yCGr_G
Cuts at: 1870 1943 2630 1870
Size: 73 687 4545
Fragments arranged by size:
4545 687 73
BamHI G'GATC_C
Cuts at: 2559 2635 3002 2559
Size: 76 367 4862
Fragments arranged by size:
4862 367 76
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2626 4081 618
Size: 325 217 627 839 1455 1842
Fragments arranged by size:
1842 1455 839 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2624 1391
Size: 1233 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2286 928
Size: 971 387 3947
Fragments arranged by size:
3947 971 387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3496 3530 1903
Size: 34 1559 34 3678
Fragments arranged by size:
3678 1559 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3197 4407 3197
Size: 1210 4095
BclI T'GATC_A
Cuts at: 1548 2188 1548
Size: 640 4665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3916 136
Size: 107 122 71 631 605 2244 1525
Fragments arranged by size:
2244 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5300 5300
Size: 5305
BlpI GC'TnA_GC
Cuts at: 2722 2722
Size: 5305
Bme1580I G_kGCm'C
Cuts at: 1790 3366 4522 1790
Size: 1576 1156 2573
Fragments arranged by size:
2573 1576 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5305
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2565 3036 3994
Size: 416 53 83 399 24 1148 471 958
Cuts at: 3994 442
Size: 1753
Fragments arranged by size:
1753 1148 958 471 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2075 1056
Size: 1019 4286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3965 1239
Size: 2726 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3430 4295 4593 3430
Size: 865 298 4142
Fragments arranged by size:
4142 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2459 3968 882
Size: 617 960 1509 2219
Fragments arranged by size:
2219 1509 960 617
BsaAI yAC'GTr
Cuts at: 493 1204 2570 3096 493
Size: 711 1366 526 2702
Fragments arranged by size:
2702 1366 711 526
BsaBI GATnn'nnATC
Cuts at: 5299 5299
Size: 5305
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3112 3494 4129 275
Size: 53 83 186 936 1579 382 635 1451
Fragments arranged by size:
1579 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3738 4136 4410 4557 4968 3738
Size: 398 274 147 411 4075
Fragments arranged by size:
4075 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5080
Fragments arranged by size:
5080 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 1186
Size: 1204 4101
BseYI C'CCAG_C
Cuts at: 2563 4508 5004 2563
Size: 1945 496 2864
Fragments arranged by size:
2864 1945 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4732
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2755 3516 4544 1540
Size: 275 186 754 761 1028 2301
Fragments arranged by size:
2301 1028 761 754 275 186
BsiHKAI G_wGCw'C
Cuts at: 3366 3451 4522 3366
Size: 85 1071 4149
Fragments arranged by size:
4149 1071 85
BsmI GAATG_Cn'
Cuts at: 2020 2816 2909 2020
Size: 796 93 4416
Fragments arranged by size:
4416 796 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2624 3366 3451 4522 1391
Size: 399 834 742 85 1071 2174
Fragments arranged by size:
2174 1071 834 742 399 85
BspEI T'CCGG_A
Cuts at: 4968 4968
Size: 5305
BspHI T'CATG_A
Cuts at: 3194 4924 3194
Size: 1730 3575
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2278 2660 844
Size: 1434 382 3489
Fragments arranged by size:
3489 1434 382
BsrBI CCG'CTC
Cuts at: 1394 1823 2061 3192 1394
Size: 429 238 1131 3507
Fragments arranged by size:
3507 1131 429 238
BsrDI GCAATG_nn'
Cuts at: 65 1248 2419 3800 3974 65
Size: 1183 1171 1381 174 1396
Fragments arranged by size:
1396 1381 1183 1171 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3949 4136 1700
Size: 110 2139 187 2869
Fragments arranged by size:
2869 2139 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5305
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5305
BssSI C'ACGA_G
Cuts at: 3365 4377 3365
Size: 1012 4293
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5305
BstBI TT'CG_AA
Cuts at: 4109 4109
Size: 5305
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5305
BstYI r'GATC_y
Cuts at: 1972 2403 2559 2635 3002 3387 3404 4856 4942
Size: 431 156 76 367 385 17 1452 86
Cuts at: 4942 4954 5300 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 431 385 367 346 156 86
76 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3814
Fragments arranged by size:
3814 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4566
Fragments arranged by size:
4566 683 56
BtsI GCAGTG_nn'
Cuts at: 2821 3615 3635 2821
Size: 794 20 4491
Fragments arranged by size:
4491 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5270
DraI TTT'AAA
Cuts at: 2609 2609
Size: 5305
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2515 4312 809
Size: 507 1199 1797 1802
Fragments arranged by size:
1802 1797 1199 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2265 2752 3641
Size: 54 1425 50 275 18 435 487 889
Cuts at: 3641 5270 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 487 435 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2752 1537
Size: 275 940 4090
Fragments arranged by size:
4090 940 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3235 1353
Size: 1882 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3892 4266 4412 387
Size: 1064 138 2303 374 146 1280
Fragments arranged by size:
2303 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3368 3965 4752 5020 1239
Size: 393 1736 597 787 268 1524
Fragments arranged by size:
1736 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2047 2131 2476 2047
Size: 84 345 4876
Fragments arranged by size:
4876 345 84
EcoRI G'AATT_C
Cuts at: 2614 2992 2614
Size: 378 4927
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2249 2249
Size: 5305
FspI TGC'GCA
Cuts at: 3811 3811
Size: 5305
HaeII r_GCGC'y
Cuts at: 1678 4452 1678
Size: 2774 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3946 3978 4020 4052 1373
Size: 32 135 32 2374 32 42 32 2626
Fragments arranged by size:
2626 2374 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2649 2895 669
Size: 988 281 711 246 3079
Fragments arranged by size:
3079 988 711 281 246
HindIII A'AGCT_T
Cuts at: 748 2238 748
Size: 1490 3815
HpaI GTT'AAC
Cuts at: 2895 2895
Size: 5305
KpnI G_GTAC'C
Cuts at: 2630 2630
Size: 5305
MfeI C'AATT_G
Cuts at: 2904 2904
Size: 5305
MluI A'CGCG_T
Cuts at: 5181 5181
Size: 5305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2422 4419 4603 1305
Size: 1117 1997 184 2007
Fragments arranged by size:
2007 1997 1117 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2267 10
Size: 54 1425 343 435 3048
Fragments arranged by size:
3048 1425 435 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3263 3622 3781 518
Size: 648 2097 359 159 2042
Fragments arranged by size:
2097 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2721 3398 4546 4791 1606
Size: 423 692 677 1148 245 2120
Fragments arranged by size:
2120 1148 692 677 423 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3814
Fragments arranged by size:
3814 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5305
NheI G'CTAG_C
Cuts at: 1052 2071 1052
Size: 1019 4286
NotI GC'GGCC_GC
Cuts at: 2752 2752
Size: 5305
NspI r_CATG'y
Cuts at: 1132 1229 2663 4208 1132
Size: 97 1434 1545 2229
Fragments arranged by size:
2229 1545 1434 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5305
PciI A'CATG_T
Cuts at: 4204 4204
Size: 5305
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2570 1108
Size: 1462 3843
PfoI T'CCnGG_A
Cuts at: 2391 2391
Size: 5305
PmeI GTTT'AAAC
Cuts at: 2609 2609
Size: 5305
PmlI CAC'GTG
Cuts at: 2570 2570
Size: 5305
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 4887 4919 3374
Size: 32 1481 32 3760
Fragments arranged by size:
3760 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2047
Size: 5305
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5305
PsiI TTA'TAA
Cuts at: 2875 2875
Size: 5305
PstI C_TGCA'G
Cuts at: 830 2493 2657 830
Size: 1663 164 3478
Fragments arranged by size:
3478 1663 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5305
SalI G'TCGA_C
Cuts at: 1936 2647 1936
Size: 711 4594
SbfI CC_TGCA'GG
Cuts at: 2657 2657
Size: 5305
ScaI AGT'ACT
Cuts at: 3553 5265 3553
Size: 1712 3593
SfcI C'TryA_G
Cuts at: 826 1045 2053 2489 2653 3788 4469 4660 826
Size: 219 1008 436 164 1135 681 191 1471
Fragments arranged by size:
1471 1135 1008 681 436 219 191 164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5305
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5305
SmaI CCC'GGG
Cuts at: 1872 2632 1872
Size: 760 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3409 4310 4572 820
Size: 197 926 1466 901 262 1553
Fragments arranged by size:
1553 1466 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3096 493
Size: 2603 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5305
SphI G_CATG'C
Cuts at: 1132 2663 1132
Size: 1531 3774
SspI AAT'ATT
Cuts at: 5 52 3229 5
Size: 47 3177 2081
Fragments arranged by size:
3177 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5305
StyI C'CwwG_G
Cuts at: 513 1065 2004 2744 513
Size: 552 939 740 3074
Fragments arranged by size:
3074 939 740 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2222 3332 3349 3502 3687 4132 325
Size: 1255 642 1110 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1110 642 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3551 5263 96
Size: 274 80 33 51 1443 1574 1712 138
Fragments arranged by size:
1712 1574 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5265 5297 5265
Size: 32 5273
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5305
XbaI T'CTAG_A
Cuts at: 2641 2641
Size: 5305
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5305
XmaI C'CCGG_G
Cuts at: 1870 2630 1870
Size: 760 4545
XmnI GAAnn'nnTTC
Cuts at: 3434 3434
Size: 5305
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3113 276
Size: 53 83 186 936 1579 2468
Fragments arranged by size:
2468 1579 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI