Restriction map for
pFN21AE9601
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BmyI
BpmI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HapII HgaI Hin1I HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflIII 494 ACATGT A^C[GA][CT]GT
AlwNI 274 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
BbsI 295 GAAGAC GAAGAC(2/6)
BfaI 38 CTAG C^TAG
BglII 141 AGATCT A^GATCT
BlpI 279 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 279 GCTGAGC GC^T[ACGT]AGC
BpuAI 295 GAAGAC GAAGAC(2/6)
BseRI 215 CTCCTC GAGGAG(10/8)
BsgI 251 GTGCAG GTGCAG(16/14)
BsiYI 75 CCTCAAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 75 CCTCAAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 497 TGTACA T^GTACA
BspLU11I 494 ACATGT A^CATGT
BsrGI 497 TGTACA T^GTACA
BstF5I 33 CATCC GGATG(2/0)
BstYI 141 AGATCT [GA]^GATC[CT]
Cac8I 305 GCCAGC GC[ACGT]^[ACGT]GC
CelII 279 GCTGAGC GC^T[ACGT]AGC
Cfr13I 14 GGCCC G^G[ACGT]CC
FauI 106 GCGGG CCCGC(4/6)
FokI 33 CATCC GGATG(9/13)
HaeII 325 AGCGCC [GA]GCGC^[CT]
HincII 234 GTTGAC GT[CT]^[GA]AC
HindII 234 GTTGAC GT[CT]^[GA]AC
MaeI 38 CTAG C^TAG
MaeIII 90 GTTAC ^GT[ACGT]AC
MflI 141 AGATCT [GA]^GATC[CT]
MseI 556 TTAA T^TAA
MspA1I 274 CAGCTG C[AC]G^C[GT]G
NspI 494 ACATGT [GA]CATG^[CT]
PleI 237 GACTC GAGTC(4/5)
PvuII 274 CAGCTG CAG^CTG
Sau96I 14 GGCCC G^G[ACGT]CC
SfcI 155 CTACAG C^T[GA][CT]AG
SmlI 321 CTTGAG C^T[CT][GA]AG
SspBI 497 TGTACA T^GTACA
StuI 134 AGGCCT AGG^CCT
TfiI 431 GATTC G^A[AT]TC
Tru9I 556 TTAA T^TAA
Tsp509I 80 AATT ^AATT
XhoII 141 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 329 CCGC CCGC(-3/-1)
106 GCGG
AfaI 498 GTAC GT^AC
537 GTAC
BsaJI 4 CCTGGG C^C[ACGT][ACGT]GG
545 CCCAGG
BssKI 4 CCTGG ^CC[ACGT]GG
546 CCAGG
BstNI 4 CCTGG CC^[AT]GG
546 CCAGG
CfoI 326 GCGC GCG^C
473 GCGC
DpnII 126 GATC ^GATC
142 GATC
EcoRII 4 CCTGG ^CC[AT]GG
546 CCAGG
HaeIII 14 GGCC GG^CC
135 GGCC
HhaI 326 GCGC GCG^C
473 GCGC
HinP1I 326 GCGC G^CGC
473 GCGC
HinfI 237 GACTC G^A[ACGT]TC
431 GATTC
HphI 109 GGTGA GGTGA(8/7)
532 GGTGA
MboI 126 GATC ^GATC
142 GATC
MboII 295 GAAGA GAAGA(8/7)
526 GAAGA
MslI 244 CATCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
501 CATAGAAATG
MvaI 4 CCTGG CC^[AT]GG
546 CCAGG
NdeII 126 GATC ^GATC
142 GATC
RsaI 498 GTAC GT^AC
537 GTAC
Sau3AI 126 GATC ^GATC
142 GATC
ScrFI 4 CCTGG CC^[ACGT]GG
546 CCAGG
SfaNI 22 GATGC GCATC(5/9)
518 GCATC
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI BbvI DdeI Fnu4HI ItaI
MnlI NlaIII TaqI TseI TspRI
TthHB8I