Restriction map for
pFN21AE9601
(Circular) (Six-base) MAPSORT of: pFN21AE9601.seq Check: 1774 from: 1 to: 5266
pFN21AE9601 5266 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3076 278
Size: 53 83 186 936 1540 2468
Fragments arranged by size:
2468 1540 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2609 1937
Size: 672 4594
Acc65I G'GTAC_C
Cuts at: 2587 2587
Size: 5266
AclI AA'CG_TT
Cuts at: 1563 3393 3766 1563
Size: 1830 373 3063
Fragments arranged by size:
3063 1830 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3329 4713 4981 1632
Size: 1697 1384 268 1917
Fragments arranged by size:
1917 1697 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5069
AflIII A'CryG_T
Cuts at: 1203 2499 4165 5142 1203
Size: 1296 1666 977 1327
Fragments arranged by size:
1666 1327 1296 977
AgeI A'CCGG_T
Cuts at: 4097 4097
Size: 5266
AhdI GACnn_n'nnGTC
Cuts at: 3995 3995
Size: 5266
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5266
AlwNI CAG_nnn'CTG
Cuts at: 2284 3009 4581 2284
Size: 725 1572 2969
Fragments arranged by size:
2969 1572 725
ApaLI G'TGCA_C
Cuts at: 3323 4479 3323
Size: 1156 4110
ApoI r'AATT_y
Cuts at: 1493 2575 2801 2953 5062 1493
Size: 1082 226 152 2109 1697
Fragments arranged by size:
2109 1697 1082 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3820 160
Size: 872 2788 1606
Fragments arranged by size:
2788 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5266
AvaI C'yCGr_G
Cuts at: 1870 1943 2591 1870
Size: 73 648 4545
Fragments arranged by size:
4545 648 73
BamHI G'GATC_C
Cuts at: 2596 2963 2596
Size: 367 4899
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2587 4042 618
Size: 325 217 627 800 1455 1842
Fragments arranged by size:
1842 1455 800 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2585 1391
Size: 1194 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2307 928
Size: 971 408 3887
Fragments arranged by size:
3887 971 408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3457 3491 1903
Size: 34 50 34 1436 34 3678
Fragments arranged by size:
3678 1436 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3158 4368 3158
Size: 1210 4056
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5266
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3877 136
Size: 107 122 71 631 605 2205 1525
Fragments arranged by size:
2205 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2146 5261 2146
Size: 3115 2151
BlpI GC'TnA_GC
Cuts at: 2285 2683 2285
Size: 398 4868
Bme1580I G_kGCm'C
Cuts at: 1790 3327 4483 1790
Size: 1537 1156 2573
Fragments arranged by size:
2573 1537 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5266
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2997 3955 442
Size: 416 53 83 399 24 1580 958 1753
Fragments arranged by size:
1753 1580 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5266
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2250 2250
Size: 5266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3926 1239
Size: 2687 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2347 3391 4256 4554 2347
Size: 1044 865 298 3059
Fragments arranged by size:
3059 1044 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3929 882
Size: 617 2430 2219
Fragments arranged by size:
2430 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3057 493
Size: 711 1853 2702
Fragments arranged by size:
2702 1853 711
BsaBI GATnn'nnATC
Cuts at: 5260 5260
Size: 5266
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3073 3455 4090 275
Size: 53 83 186 936 1540 382 635 1451
Fragments arranged by size:
1540 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3699 4097 4371 4518 4929 3699
Size: 398 274 147 411 4036
Fragments arranged by size:
4036 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5041
Fragments arranged by size:
5041 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2211 1186
Size: 1025 4241
BseYI C'CCAG_C
Cuts at: 2486 4469 4965 2486
Size: 1983 496 2787
Fragments arranged by size:
2787 1983 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2277 1163
Size: 573 541 4152
Fragments arranged by size:
4152 573 541
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2716 3477 4505 1540
Size: 275 186 715 761 1028 2301
Fragments arranged by size:
2301 1028 761 715 275 186
BsiHKAI G_wGCw'C
Cuts at: 3327 3412 4483 3327
Size: 85 1071 4110
Fragments arranged by size:
4110 1071 85
BsmI GAATG_Cn'
Cuts at: 2777 2870 2777
Size: 93 5173
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2585 3327 3412 4483 1391
Size: 399 795 742 85 1071 2174
Fragments arranged by size:
2174 1071 795 742 399 85
BspEI T'CCGG_A
Cuts at: 4929 4929
Size: 5266
BspHI T'CATG_A
Cuts at: 3155 4885 3155
Size: 1730 3536
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2621 844
Size: 1777 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3153 1394
Size: 429 1330 3507
Fragments arranged by size:
3507 1330 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3761 3935 65
Size: 1183 2513 174 1396
Fragments arranged by size:
2513 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3910 4097 1700
Size: 110 2100 187 2869
Fragments arranged by size:
2869 2100 187 110
BsrGI T'GTAC_A
Cuts at: 96 2502 96
Size: 2406 2860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5266
BssSI C'ACGA_G
Cuts at: 3326 4338 3326
Size: 1012 4254
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5266
BstBI TT'CG_AA
Cuts at: 4070 4070
Size: 5266
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5266
BstYI r'GATC_y
Cuts at: 1972 2146 2596 2963 3348 3365 4817 4903 4915
Size: 174 450 367 385 17 1452 86 12
Cuts at: 4915 5261 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 450 385 367 346 174 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4714
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4527
Fragments arranged by size:
4527 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 2782 3576 3596 2265
Size: 517 794 20 3935
Fragments arranged by size:
3935 794 517 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5231
DraI TTT'AAA
Cuts at: 2570 2570
Size: 5266
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4273 809
Size: 507 2957 1802
Fragments arranged by size:
2957 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2713 3602 5231
Size: 54 1425 50 275 18 883 889 1629
Cuts at: 5231 8
Size: 43
Fragments arranged by size:
1629 1425 889 883 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2713 1537
Size: 275 901 4090
Fragments arranged by size:
4090 901 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3196 1353
Size: 1843 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3853 4227 4373 387
Size: 1064 138 2264 374 146 1280
Fragments arranged by size:
2264 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3329 3926 4713 4981 1239
Size: 393 1697 597 787 268 1524
Fragments arranged by size:
1697 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2575 2953 2575
Size: 378 4888
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2444 2444
Size: 5266
FspI TGC'GCA
Cuts at: 3772 3772
Size: 5266
HaeII r_GCGC'y
Cuts at: 1678 2334 4413 1678
Size: 656 2079 2531
Fragments arranged by size:
2531 2079 656
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2233 2265 3907 3939 3981
Size: 32 135 32 661 32 1642 32 42
Cuts at: 3981 4013 1373
Size: 32 2626
Fragments arranged by size:
2626 1642 661 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2241 2610 2856 669
Size: 988 281 303 369 246 3079
Fragments arranged by size:
3079 988 369 303 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5266
HpaI GTT'AAC
Cuts at: 2856 2856
Size: 5266
KpnI G_GTAC'C
Cuts at: 2591 2591
Size: 5266
MfeI C'AATT_G
Cuts at: 2865 2865
Size: 5266
MluI A'CGCG_T
Cuts at: 5142 5142
Size: 5266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4380 4564 1305
Size: 3075 184 2007
Fragments arranged by size:
3075 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3444
Fragments arranged by size:
3444 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2253 2510 3224 3583 3742 518
Size: 648 1087 257 714 359 159 2042
Fragments arranged by size:
2042 1087 714 648 359 257 159
MspA1I CmG'CkG
Cuts at: 1606 2281 2682 3359 4507 4752 1606
Size: 675 401 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 675 401 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4714
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5266
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5266
NotI GC'GGCC_GC
Cuts at: 2713 2713
Size: 5266
NspI r_CATG'y
Cuts at: 1132 1229 2503 2624 4169 1132
Size: 97 1274 121 1545 2229
Fragments arranged by size:
2229 1545 1274 121 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5266
PciI A'CATG_T
Cuts at: 2499 4165 2499
Size: 1666 3600
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5266
PmeI GTTT'AAAC
Cuts at: 2570 2570
Size: 5266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3335 3367 4848 4880 3335
Size: 32 1481 32 3721
Fragments arranged by size:
3721 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5266
PsiI TTA'TAA
Cuts at: 2836 2836
Size: 5266
PstI C_TGCA'G
Cuts at: 830 2618 830
Size: 1788 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5266
PvuII CAG'CTG
Cuts at: 2281 2281
Size: 5266
SalI G'TCGA_C
Cuts at: 1936 2608 1936
Size: 672 4594
SbfI CC_TGCA'GG
Cuts at: 2618 2618
Size: 5266
ScaI AGT'ACT
Cuts at: 3514 5226 3514
Size: 1712 3554
SfcI C'TryA_G
Cuts at: 826 1045 2160 2614 3749 4430 4621 826
Size: 219 1115 454 1135 681 191 1471
Fragments arranged by size:
1471 1135 1115 681 454 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5266
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5266
SmaI CCC'GGG
Cuts at: 1872 2593 1872
Size: 721 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2326 3370 4271 4533 820
Size: 197 926 383 1044 901 262 1553
Fragments arranged by size:
1553 1044 926 901 383 262 197
SnaBI TAC'GTA
Cuts at: 493 3057 493
Size: 2564 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5266
SphI G_CATG'C
Cuts at: 1132 2624 1132
Size: 1492 3774
SspI AAT'ATT
Cuts at: 5 52 3190 5
Size: 47 3138 2081
Fragments arranged by size:
3138 2081 47
StuI AGG'CCT
Cuts at: 1517 2141 1517
Size: 624 4642
StyI C'CwwG_G
Cuts at: 513 1065 2705 513
Size: 552 1640 3074
Fragments arranged by size:
3074 1640 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3293 3310 3463 3648 4093 325
Size: 1255 1713 17 153 185 445 1498
Fragments arranged by size:
1713 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2502 2541 3512
Size: 274 80 33 51 1443 525 39 971
Cuts at: 3512 5224 96
Size: 1712 138
Fragments arranged by size:
1712 1443 971 525 274 138 80 51
39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5226 5258 5226
Size: 32 5234
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5266
XbaI T'CTAG_A
Cuts at: 2602 2602
Size: 5266
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5266
XmaI C'CCGG_G
Cuts at: 1870 2591 1870
Size: 721 4545
XmnI GAAnn'nnTTC
Cuts at: 3395 3395
Size: 5266
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3074 276
Size: 53 83 186 936 1540 2468
Fragments arranged by size:
2468 1540 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI