Restriction map for
pFN21AE9222
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwNI AocI
Aor51HI AscI Asp718I AspEI AspI
AviII BamHI BbeI BbrPI BbvCI
BfrI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BssHII BssSI Bst1107I BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRV FseI FspI
HaeII Hin1I HindIII KasI KpnI
KspI MaeII MluI MroI NarI
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Alw44I 1426 GTGCAC G^TGCAC
ApaI 2707 GGGCCC GGGCC^C
ApaLI 1426 GTGCAC G^TGCAC
Asp700I 895 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 54 CTCGAG C^[CT]CG[GA]G
AvrII 2979 CCTAGG C^CTAGG
BalI 478 TGGCCA TGG^CCA
BanI 1743 GGTGCC G^G[CT][GA]CC
BcgI 2749 CGAAGAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 945 GTATCC GTATCC(6/5)
BglII 363 AGATCT A^GATCT
BlnI 2979 CCTAGG C^CTAGG
BsaBI 2458 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 865 GTGCAG GTGCAG(16/14)
BsiEI 29 CGGCCG CG[GA][CT]^CG
BsoBI 54 CTCGAG C^[CT]CG[GA]G
BspMI 1365 ACCTGC ACCTGC(4/8)
BsrFI 31 GCCGGC [GA]^CCGG[CT]
BstAPI 385 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 35 GCGC GCG^C
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
DraI 1674 TTTAAA TTT^AAA
EagI 29 CGGCCG C^GGCCG
EclXI 29 CGGCCG C^GGCCG
Eco52I 29 CGGCCG C^GGCCG
EcoNI 1734 CCTTCGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 772 GAATTC G^AATTC
EcoT22I 376 ATGCAT ATGCA^T
HhaI 35 GCGC GCG^C
HinP1I 35 GCGC G^CGC
HpaI 829 GTTAAC GTT^AAC
MamI 2458 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 544 CAATTG C^AATTG
MluNI 478 TGGCCA TGG^CCA
MscI 478 TGGCCA TGG^CCA
MunI 544 CAATTG C^AATTG
NaeI 31 GCCGGC GCC^GGC
NdeI 1402 CATATG CA^TATG
NgoMIV 31 GCCGGC G^CCGGC
NheI 816 GCTAGC G^CTAGC
NsiI 376 ATGCAT ATGCA^T
PaeR7I 54 CTCGAG C^TCGAG
PflMI 452 CCAATGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 104 AGGACCC [GA]G^G[AT]CC[CT]
ScaI 1693 AGTACT AGT^ACT
SexAI 2663 ACCTGGT A^CC[AT]GGT
Van91I 452 CCAATGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3003 TCTAGA T^CTAGA
XcmI 2891 CCAAGGATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 54 CTCGAG C^TCGAG
XmnI 895 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 27 CGCG CG^CG
36 CGCG
AflIII 508 ACATGT A^C[GA][CT]GT
1190 ACATGT
AspHI 1426 GTGCAC G[AT]GC[AT]^C
2934 GTGCTC
AvaII 105 GGACC G^G[AT]CC
2530 GGTCC
BanII 193 GAGCCC G[GA]GC[CT]^C
2707 GGGCCC
BclI 2808 TGATCA T^GATCA
2849 TGATCA
BcnI 1323 CCCGG CC^[GC]GG
2715 CCGGG
BglI 31 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
480 GCCAGTTGGGC
BsaI 931 GAGACC GGTCTC(1/5)
2347 GAGACC
BsiHKAI 1426 GTGCAC G[AT]GC[AT]^C
2934 GTGCTC
BsmFI 836 GTCCC GGGAC(10/14)
1122 GTCCC
Bsp1407I 1193 TGTACA T^GTACA
1937 TGTACA
BspLU11I 508 ACATGT A^CATGT
1190 ACATGT
BsrBI 1318 CCGCTC CCGCTC(-3/-3)
2277 GAGCGG
BsrDI 315 CATTGC GCAATG(2/0)
2684 GCAATG
BsrGI 1193 TGTACA T^GTACA
1937 TGTACA
BstUI 27 CGCG CG^CG
36 CGCG
DraIII 1036 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2294 CACTGAGTG
FauI 1317 CCCGC CCCGC(4/6)
2711 CCCGC
FbaI 2808 TGATCA T^GATCA
2849 TGATCA
HgaI 25 GACGC GACGC(5/10)
805 GACGC
HincII 829 GTTAAC GT[CT]^[GA]AC
1086 GTTGAC
HindII 829 GTTAAC GT[CT]^[GA]AC
1086 GTTGAC
MvnI 27 CGCG CG^CG
36 CGCG
NciI 1323 CCCGG CC^[GC]GG
2715 CCGGG
NcoI 998 CCATGG C^CATGG
1562 CCATGG
SfaNI 375 GATGC GCATC(5/9)
2905 GATGC
SphI 1758 GCATGC GCATG^C
2211 GCATGC
SspBI 1193 TGTACA T^GTACA
1937 TGTACA
StuI 1905 AGGCCT AGG^CCT
2616 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmI Bsp1286I BspHI BsrI BssKI
BstBI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
NspV PleI PshBI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfcI SfuI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII