Restriction map for
pFN21AE9222
(Circular) (Six-base) MAPSORT of: pFN21AE9222.seq Check: 106 from: 1 to: 7840
pFN21AE9222 7840 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5650 278
Size: 53 83 186 936 4114 2468
Fragments arranged by size:
4114 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2332 2703 4398 5183 1937
Size: 395 371 1695 785 4594
Fragments arranged by size:
4594 1695 785 395 371
Acc65I G'GTAC_C
Cuts at: 5161 5161
Size: 7840
AclI AA'CG_TT
Cuts at: 1563 5967 6340 1563
Size: 4404 373 3063
Fragments arranged by size:
4404 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2156 2253 2294 2642 2919 2942 3411 3540
Size: 524 97 41 348 277 23 469 129
Cuts at: 3540 3623 4158 4367 4544 5903 7287 7555 1632
Size: 83 535 209 177 1359 1384 268 1917
Fragments arranged by size:
1917 1384 1359 535 524 469 348 277
268 209 177 129 97 83 41 23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7643
AflIII A'CryG_T
Cuts at: 1203 2513 3195 6739 7716 1203
Size: 1310 682 3544 977 1327
Fragments arranged by size:
3544 1327 1310 977 682
AgeI A'CCGG_T
Cuts at: 6671 6671
Size: 7840
AhdI GACnn_n'nnGTC
Cuts at: 6569 6569
Size: 7840
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7840
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4977 1184
Size: 3793 4047
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4842 4874 4842
Size: 32 7808
AlwNI CAG_nnn'CTG
Cuts at: 5583 7155 5583
Size: 1572 6268
ApaI G_GGCC'C
Cuts at: 4716 4716
Size: 7840
ApaLI G'TGCA_C
Cuts at: 3431 5897 7053 3431
Size: 2466 1156 4218
Fragments arranged by size:
4218 2466 1156
ApoI r'AATT_y
Cuts at: 1493 2349 2558 2696 2777 3147 3164 3264 3353
Size: 856 209 138 81 370 17 100 89
Cuts at: 3353 3619 5149 5375 5527 7636 1493
Size: 266 1530 226 152 2109 1697
Fragments arranged by size:
2109 1697 1530 856 370 266 226 209
152 138 100 89 81 17
AseI AT'TA_AT
Cuts at: 160 1032 3948 4836 4881 6394 160
Size: 872 2916 888 45 1513 1606
Fragments arranged by size:
2916 1606 1513 888 872 45
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7840
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 5165 1870
Size: 73 116 3106 4545
Fragments arranged by size:
4545 3106 116 73
AvrII C'CTAG_G
Cuts at: 4984 4984
Size: 7840
BamHI G'GATC_C
Cuts at: 5170 5537 5170
Size: 367 7473
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3748 5161 6616 618
Size: 325 217 627 1961 1413 1455 1842
Fragments arranged by size:
1961 1842 1455 1413 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2202 4716 5159 1391
Size: 811 2514 443 4072
Fragments arranged by size:
4072 2514 811 443
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2572 3555 3629 928
Size: 971 673 983 74 5139
Fragments arranged by size:
5139 983 971 673 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4743 4777 6031 6065 1903
Size: 34 2806 34 1254 34 3678
Fragments arranged by size:
3678 2806 1254 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2961 5732 6942 2961
Size: 2771 1210 3859
Fragments arranged by size:
3859 2771 1210
BclI T'GATC_A
Cuts at: 1548 4813 4854 1548
Size: 3265 41 4534
Fragments arranged by size:
4534 3265 41
BfrBI ATG'CAT
Cuts at: 2383 2383
Size: 7840
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2042 2491 6451
Size: 107 122 71 631 605 370 449 3960
Cuts at: 6451 136
Size: 1525
Fragments arranged by size:
3960 1525 631 605 449 370 122 107
71
BglII A'GATC_T
Cuts at: 2368 7835 2368
Size: 5467 2373
BlpI GC'TnA_GC
Cuts at: 5257 5257
Size: 7840
Bme1580I G_kGCm'C
Cuts at: 1790 3435 4716 5901 7057 1790
Size: 1645 1281 1185 1156 2573
Fragments arranged by size:
2573 1645 1281 1185 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7840
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2196 5571 6529
Size: 416 53 83 399 24 779 3375 958
Cuts at: 6529 442
Size: 1753
Fragments arranged by size:
3375 1753 958 779 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2825 1056
Size: 1769 6071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2357 2485 3069 4312 4790 6500 1239
Size: 1118 128 584 1243 478 1710 2579
Fragments arranged by size:
2579 1710 1243 1118 584 478 128
Bpu10I CC'TnA_GC
Cuts at: 3304 3304
Size: 7840
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3091 3830 4019 4497 5965 6830 7128 3091
Size: 739 189 478 1468 865 298 3803
Fragments arranged by size:
3803 1468 865 739 478 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2930 4346 6503 882
Size: 617 1431 1416 2157 2219
Fragments arranged by size:
2219 2157 1431 1416 617
BsaAI yAC'GTr
Cuts at: 493 1204 5631 493
Size: 711 4427 2702
Fragments arranged by size:
4427 2702 711
BsaBI GATnn'nnATC
Cuts at: 4467 7834 4467
Size: 3367 4473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5647 6029 6664 275
Size: 53 83 186 936 4114 382 635 1451
Fragments arranged by size:
4114 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6273 6671 6945 7092 7503 6273
Size: 398 274 147 411 6610
Fragments arranged by size:
6610 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2775 2805 1178
Size: 30 165 30 1372 30 6213
Fragments arranged by size:
6213 1372 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2482 3626 3664 4097 4129 1186
Size: 1296 1144 38 433 32 4897
Fragments arranged by size:
4897 1296 1144 433 38 32
BseYI C'CCAG_C
Cuts at: 3310 7043 7539 3310
Size: 3733 496 3611
Fragments arranged by size:
3733 3611 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2891 1163
Size: 573 1155 6112
Fragments arranged by size:
6112 1155 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 5290 6051 7079 1540
Size: 275 186 36 3253 761 1028 2301
Fragments arranged by size:
3253 2301 1028 761 275 186 36
BsiHKAI G_wGCw'C
Cuts at: 3435 4943 5901 5986 7057 3435
Size: 1508 958 85 1071 4218
Fragments arranged by size:
4218 1508 1071 958 85
BsmI GAATG_Cn'
Cuts at: 2103 2738 2813 4310 4783 4866 5351 5444 2103
Size: 635 75 1497 473 83 485 93 4499
Fragments arranged by size:
4499 1497 635 485 473 93 83 75
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 3435 4716 4943 5159 5901 5986
Size: 399 412 1233 1281 227 216 742 85
Cuts at: 5986 7057 1391
Size: 1071 2174
Fragments arranged by size:
2174 1281 1233 1071 742 412 399 227
216 85
BspEI T'CCGG_A
Cuts at: 7503 7503
Size: 7840
BspHI T'CATG_A
Cuts at: 2290 3778 4923 5729 7459 2290
Size: 1488 1145 806 1730 2671
Fragments arranged by size:
2671 1730 1488 1145 806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3379 5195 844
Size: 2535 1816 3489
Fragments arranged by size:
3489 2535 1816
BsrBI CCG'CTC
Cuts at: 1394 1823 3325 4284 5727 1394
Size: 429 1502 959 1443 3507
Fragments arranged by size:
3507 1502 1443 959 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2319 4696 6335 6509 65
Size: 1183 1071 2377 1639 174 1396
Fragments arranged by size:
2377 1639 1396 1183 1071 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 6484 6671 1700
Size: 110 226 4448 187 2869
Fragments arranged by size:
4448 2869 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 3198 3942 96
Size: 3102 744 3994
Fragments arranged by size:
3994 3102 744
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7840
BssSI C'ACGA_G
Cuts at: 5900 6912 5900
Size: 1012 6828
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2396 794
Size: 1602 6238
BstBI TT'CG_AA
Cuts at: 2563 3220 3241 6644 2563
Size: 657 21 3403 3759
Fragments arranged by size:
3759 3403 657 21
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7840
BstYI r'GATC_y
Cuts at: 1972 2368 2722 5011 5170 5537 5922 5939 7391
Size: 396 354 2289 159 367 385 17 1452
Cuts at: 7391 7477 7489 7835 1972
Size: 86 12 346 1977
Fragments arranged by size:
2289 1977 1452 396 385 367 354 346
159 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2053 3003 3567 513
Size: 552 988 950 564 4786
Fragments arranged by size:
4786 988 950 564 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7101
Fragments arranged by size:
7101 683 56
BtsI GCAGTG_nn'
Cuts at: 3433 5356 6150 6170 3433
Size: 1923 794 20 5103
Fragments arranged by size:
5103 1923 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7840
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7805
DraI TTT'AAA
Cuts at: 3681 5144 3681
Size: 1463 6377
DraIII CAC_nnn'GTG
Cuts at: 3046 4304 3046
Size: 1258 6582
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6847 809
Size: 507 5531 1802
Fragments arranged by size:
5531 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2050 2483
Size: 54 1425 50 275 18 204 16 433
Cuts at: 2483 2944 5287 6176 7805 8
Size: 461 2343 889 1629 43
Fragments arranged by size:
2343 1629 1425 889 461 433 275 204
54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2034 5287 1537
Size: 275 222 3253 4090
Fragments arranged by size:
4090 3253 275 222
EarI CTCTTCn'nnn_
Cuts at: 1353 2359 2961 3490 4089 4189 4274 4551 5770
Size: 1006 602 529 599 100 85 277 1219
Cuts at: 5770 1353
Size: 3423
Fragments arranged by size:
3423 1219 1006 602 599 529 277 100
85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6427 6801 6947 387
Size: 1064 138 4838 374 146 1280
Fragments arranged by size:
4838 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2253 2294 2357 2485 2642 2919
Size: 393 524 97 41 63 128 157 277
Cuts at: 2919 2942 3069 3411 3540 3623 4158 4312 4367
Size: 23 127 342 129 83 535 154 55
Cuts at: 4367 4544 4790 5903 6500 7287 7555 1239
Size: 177 246 1113 597 787 268 1524
Fragments arranged by size:
1524 1113 787 597 535 524 393 342
277 268 246 177 157 154 129 128
127 97 83 63 55 41 23
EcoNI CCTnn'n_nnAGG
Cuts at: 3743 3743
Size: 7840
EcoO109I rG'GnC_Cy
Cuts at: 2110 4712 4713 2110
Size: 2602 1 5237
Fragments arranged by size:
5237 2602 1
EcoRI G'AATT_C
Cuts at: 2777 5149 5527 2777
Size: 2372 378 5090
Fragments arranged by size:
5090 2372 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2324 4676 2324
Size: 2352 5488
FspI TGC'GCA
Cuts at: 6346 6346
Size: 7840
HaeII r_GCGC'y
Cuts at: 1678 6987 1678
Size: 5309 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2559 2591 3440 3472 3541
Size: 32 135 32 987 32 849 32 69
Cuts at: 3541 3573 3989 4021 4472 4504 4802 4834 6481
Size: 32 416 32 451 32 298 32 1647
Cuts at: 6481 6513 6555 6587 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1647 987 849 451 416 298 135
69 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2836 3093 5184 5430 669
Size: 988 281 898 257 2091 246 3079
Fragments arranged by size:
3079 2091 988 898 281 257 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7840
HpaI GTT'AAC
Cuts at: 2836 5430 2836
Size: 2594 5246
KpnI G_GTAC'C
Cuts at: 5165 5165
Size: 7840
MfeI C'AATT_G
Cuts at: 2549 5439 2549
Size: 2890 4950
MluI A'CGCG_T
Cuts at: 7716 7716
Size: 7840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5072 6954 7138 1305
Size: 3767 1882 184 2007
Fragments arranged by size:
3767 2007 1882 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2485 10
Size: 54 1425 343 653 5365
Fragments arranged by size:
5365 1425 653 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 3407 3572 4550 4838 5798 6157
Size: 648 1479 762 165 978 288 960 359
Cuts at: 6157 6316 518
Size: 159 2042
Fragments arranged by size:
2042 1479 978 960 762 648 359 288
165 159
MspA1I CmG'CkG
Cuts at: 1606 2875 4978 5096 5256 5933 7081 7326 1606
Size: 1269 2103 118 160 677 1148 245 2120
Fragments arranged by size:
2120 2103 1269 1148 677 245 160 118
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 7840
NcoI C'CATG_G
Cuts at: 513 1065 3003 3567 513
Size: 552 1938 564 4786
Fragments arranged by size:
4786 1938 564 552
NdeI CA'TA_TG
Cuts at: 387 3408 387
Size: 3021 4819
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 7840
NheI G'CTAG_C
Cuts at: 1052 2821 1052
Size: 1769 6071
NotI GC'GGCC_GC
Cuts at: 5287 5287
Size: 7840
NsiI A_TGCA'T
Cuts at: 2385 2385
Size: 7840
NspI r_CATG'y
Cuts at: 1132 1229 2517 3199 3337 3767 4220 4391 5198
Size: 97 1288 682 138 430 453 171 807
Cuts at: 5198 6743 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1288 807 682 453 430 171
138 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7840
PciI A'CATG_T
Cuts at: 2513 3195 6739 2513
Size: 682 3544 3614
Fragments arranged by size:
3614 3544 682
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2463 1108
Size: 1355 6485
PfoI T'CCnGG_A
Cuts at: 2793 3327 2793
Size: 534 7306
PmeI GTTT'AAAC
Cuts at: 5144 5144
Size: 7840
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5909 5941 7422 7454 5909
Size: 32 1481 32 6295
Fragments arranged by size:
6295 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 2110
Size: 7840
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7840
PsiI TTA'TAA
Cuts at: 5410 5410
Size: 7840
PspOMI G'GGCC_C
Cuts at: 4712 4712
Size: 7840
PspXI vC'TCGA_Gb
Cuts at: 2059 2059
Size: 7840
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3654 3686 3654
Size: 32 7808
PstI C_TGCA'G
Cuts at: 830 2022 2401 2716 3273 4977 5023 5192 830
Size: 1192 379 315 557 1704 46 169 3478
Fragments arranged by size:
3478 1704 1192 557 379 315 169 46
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7840
PvuII CAG'CTG
Cuts at: 2875 4978 5096 2875
Size: 2103 118 5619
Fragments arranged by size:
5619 2103 118
SalI G'TCGA_C
Cuts at: 1936 5182 1936
Size: 3246 4594
SapI GCTCTTCn'nnn_
Cuts at: 3490 4089 4274 3490
Size: 599 185 7056
Fragments arranged by size:
7056 599 185
SbfI CC_TGCA'GG
Cuts at: 5192 5192
Size: 7840
ScaI AGT'ACT
Cuts at: 3700 6088 7800 3700
Size: 2388 1712 3740
Fragments arranged by size:
3740 2388 1712
SexAI A'CCwGG_T
Cuts at: 4668 4668
Size: 7840
SfcI C'TryA_G
Cuts at: 826 1045 2018 2220 2397 2712 3269 4973 5019
Size: 219 973 202 177 315 557 1704 46
Cuts at: 5019 5188 6323 7004 7195 826
Size: 169 1135 681 191 1471
Fragments arranged by size:
1704 1471 1135 973 681 557 315 219
202 191 177 169 46
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7840
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7840
SmaI CCC'GGG
Cuts at: 1872 5167 1872
Size: 3295 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2059 3106 3845 4034 4512 5944
Size: 197 926 116 1047 739 189 478 1432
Cuts at: 5944 6845 7107 820
Size: 901 262 1553
Fragments arranged by size:
1553 1432 1047 926 901 739 478 262
197 189 116
SnaBI TAC'GTA
Cuts at: 493 5631 493
Size: 5138 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7840
SphI G_CATG'C
Cuts at: 1132 3767 4220 5198 1132
Size: 2635 453 978 3774
Fragments arranged by size:
3774 2635 978 453
SspI AAT'ATT
Cuts at: 5 52 5764 5
Size: 47 5712 2081
Fragments arranged by size:
5712 2081 47
StuI AGG'CCT
Cuts at: 1517 3912 4623 1517
Size: 2395 711 4734
Fragments arranged by size:
4734 2395 711
StyI C'CwwG_G
Cuts at: 513 1065 3003 3130 3459 3567 4896 4948 4984
Size: 552 1938 127 329 108 1329 52 36
Cuts at: 4984 5279 513
Size: 295 3074
Fragments arranged by size:
3074 1938 1329 552 329 295 127 108
52 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3549 4435 5867 5884 6037 6222 6667
Size: 1255 1969 886 1432 17 153 185 445
Cuts at: 6667 325
Size: 1498
Fragments arranged by size:
1969 1498 1432 1255 886 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2599 3198 3362
Size: 274 80 33 51 1443 622 599 164
Cuts at: 3362 3467 3473 3698 3723 3849 3942 4560 6086
Size: 105 6 225 25 126 93 618 1526
Cuts at: 6086 7798 96
Size: 1712 138
Fragments arranged by size:
1712 1526 1443 622 618 599 274 225
164 138 126 105 93 80 51 33
25 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2775 2807 7800 7832 2775
Size: 32 4993 32 2783
Fragments arranged by size:
4993 2783 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7840
XbaI T'CTAG_A
Cuts at: 5008 5176 5008
Size: 168 7672
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4903 4903
Size: 7840
XhoI C'TCGA_G
Cuts at: 1943 2059 1943
Size: 116 7724
XmaI C'CCGG_G
Cuts at: 1870 5165 1870
Size: 3295 4545
XmnI GAAnn'nnTTC
Cuts at: 2904 5969 2904
Size: 3065 4775
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5648 276
Size: 53 83 186 936 4114 2468
Fragments arranged by size:
4114 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BbvCI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI
KasI NarI NruI PacI PmlI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI