(Linear) (Six-base) MAPSORT of: KIEE9222.seq Check: 206 from: 1 to: 3135 KIEE9222.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AccI GT'mk_AC Cuts at: 0 327 698 2393 3135 Size: 327 371 1695 742 Fragments arranged by size: 1695 742 371 327 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 248 289 637 914 937 1406 1535 Size: 151 97 41 348 277 23 469 129 Cuts at: 1535 1618 2153 2362 2539 3135 Size: 83 535 209 177 596 Fragments arranged by size: 596 535 469 348 277 209 177 151 129 97 83 41 23 AflIII A'CryG_T Cuts at: 0 508 1190 3135 Size: 508 682 1945 Fragments arranged by size: 1945 682 508 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2972 3135 Size: 2972 163 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2837 2869 3135 Size: 2837 32 266 Fragments arranged by size: 2837 266 32 ApaI G_GGCC'C Cuts at: 0 2711 3135 Size: 2711 424 ApaLI G'TGCA_C Cuts at: 0 1426 3135 Size: 1426 1709 ApoI r'AATT_y Cuts at: 0 344 553 691 772 1142 1159 1259 1348 Size: 344 209 138 81 370 17 100 89 Cuts at: 1348 1614 3135 Size: 266 1521 Fragments arranged by size: 1521 370 344 266 209 138 100 89 81 17 AseI AT'TA_AT Cuts at: 0 1943 2831 2876 3135 Size: 1943 888 45 259 Fragments arranged by size: 1943 888 259 45 AvaI C'yCGr_G Cuts at: 0 54 3135 Size: 54 3081 AvrII C'CTAG_G Cuts at: 0 2979 3135 Size: 2979 156 BanI G'GyrC_C Cuts at: 0 1743 3135 Size: 1743 1392 BanII G_rGCy'C Cuts at: 0 197 2711 3135 Size: 197 2514 424 Fragments arranged by size: 2514 424 197 BbsI GAAGACnn'nnnn_ Cuts at: 0 567 1550 1624 3135 Size: 567 983 74 1511 Fragments arranged by size: 1511 983 567 74 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2738 2772 3135 Size: 2738 34 363 Fragments arranged by size: 2738 363 34 BciVI GTATCCnnnnn_n' Cuts at: 0 956 3135 Size: 956 2179 BclI T'GATC_A Cuts at: 0 2808 2849 3135 Size: 2808 41 286 Fragments arranged by size: 2808 286 41 BfrBI ATG'CAT Cuts at: 0 378 3135 Size: 378 2757 BglI GCCn_nnn'nGGC Cuts at: 0 37 486 3135 Size: 37 449 2649 Fragments arranged by size: 2649 449 37 BglII A'GATC_T Cuts at: 0 363 3135 Size: 363 2772 Bme1580I G_kGCm'C Cuts at: 0 1430 2711 3135 Size: 1430 1281 424 Fragments arranged by size: 1430 1281 424 BmrI ACTGGGnnnn_n' Cuts at: 0 191 3135 Size: 191 2944 BmtI G_CTAG'C Cuts at: 0 820 3135 Size: 820 2315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 480 1064 2307 2785 3135 Size: 352 128 584 1243 478 350 Fragments arranged by size: 1243 584 478 352 350 128 Bpu10I CC'TnA_GC Cuts at: 0 1299 3135 Size: 1299 1836 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1086 1825 2014 2492 3135 Size: 1086 739 189 478 643 Fragments arranged by size: 1086 739 643 478 189 BsaI GGTCTCn'nnnn_ Cuts at: 0 925 2341 3135 Size: 925 1416 794 Fragments arranged by size: 1416 925 794 BsaBI GATnn'nnATC Cuts at: 0 2462 3135 Size: 2462 673 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 770 800 3135 Size: 770 30 2335 Fragments arranged by size: 2335 770 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 477 1621 1659 2092 2124 3135 Size: 477 1144 38 433 32 1011 Fragments arranged by size: 1144 1011 477 433 38 32 BseYI C'CCAG_C Cuts at: 0 1305 3135 Size: 1305 1830 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 886 3135 Size: 886 2249 BsiEI CG_ry'CG Cuts at: 0 32 3135 Size: 32 3103 BsiHKAI G_wGCw'C Cuts at: 0 1430 2938 3135 Size: 1430 1508 197 Fragments arranged by size: 1508 1430 197 BsmI GAATG_Cn' Cuts at: 0 98 733 808 2305 2778 2861 3135 Size: 98 635 75 1497 473 83 274 Fragments arranged by size: 1497 635 473 274 98 83 75 Bsp1286I G_dGCh'C Cuts at: 0 197 1430 2711 2938 3135 Size: 197 1233 1281 227 197 Fragments arranged by size: 1281 1233 227 197 197 BspHI T'CATG_A Cuts at: 0 285 1773 2918 3135 Size: 285 1488 1145 217 Fragments arranged by size: 1488 1145 285 217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1374 3135 Size: 1374 1761 BsrBI CCG'CTC Cuts at: 0 1320 2279 3135 Size: 1320 959 856 Fragments arranged by size: 1320 959 856 BsrDI GCAATG_nn' Cuts at: 0 314 2691 3135 Size: 314 2377 444 Fragments arranged by size: 2377 444 314 BsrFI r'CCGG_y Cuts at: 0 31 3135 Size: 31 3104 BsrGI T'GTAC_A Cuts at: 0 1193 1937 3135 Size: 1193 744 1198 Fragments arranged by size: 1198 1193 744 BstAPI GCAn_nnn'nTGC Cuts at: 0 391 3135 Size: 391 2744 BstBI TT'CG_AA Cuts at: 0 558 1215 1236 3135 Size: 558 657 21 1899 Fragments arranged by size: 1899 657 558 21 BstYI r'GATC_y Cuts at: 0 363 717 3006 3135 Size: 363 354 2289 129 Fragments arranged by size: 2289 363 354 129 BtgI C'CryG_G Cuts at: 0 48 998 1562 3135 Size: 48 950 564 1573 Fragments arranged by size: 1573 950 564 48 BtsI GCAGTG_nn' Cuts at: 0 1428 3135 Size: 1428 1707 DraI TTT'AAA Cuts at: 0 1676 3135 Size: 1676 1459 DraIII CAC_nnn'GTG Cuts at: 0 1041 2299 3135 Size: 1041 1258 836 Fragments arranged by size: 1258 1041 836 EaeI y'GGCC_r Cuts at: 0 29 45 478 939 3135 Size: 29 16 433 461 2196 Fragments arranged by size: 2196 461 433 29 16 EagI C'GGCC_G Cuts at: 0 29 3135 Size: 29 3106 EarI CTCTTCn'nnn_ Cuts at: 0 354 956 1485 2084 2184 2269 2546 3135 Size: 354 602 529 599 100 85 277 589 Fragments arranged by size: 602 599 589 529 354 277 100 85 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 248 289 352 480 637 914 937 Size: 151 97 41 63 128 157 277 23 Cuts at: 937 1064 1406 1535 1618 2153 2307 2362 2539 Size: 127 342 129 83 535 154 55 177 Cuts at: 2539 2785 3135 Size: 246 350 Fragments arranged by size: 535 350 342 277 246 177 157 154 151 129 128 127 97 83 63 55 41 23 EcoNI CCTnn'n_nnAGG Cuts at: 0 1738 3135 Size: 1738 1397 EcoO109I rG'GnC_Cy Cuts at: 0 105 2707 2708 3135 Size: 105 2602 1 427 Fragments arranged by size: 2602 427 105 1 EcoRI G'AATT_C Cuts at: 0 772 3135 Size: 772 2363 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 319 2671 3135 Size: 319 2352 464 Fragments arranged by size: 2352 464 319 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 554 586 1435 1467 1536 1568 1984 2016 Size: 554 32 849 32 69 32 416 32 Cuts at: 2016 2467 2499 2797 2829 3135 Size: 451 32 298 32 306 Fragments arranged by size: 849 554 451 416 306 298 69 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 831 1088 3135 Size: 831 257 2047 Fragments arranged by size: 2047 831 257 HpaI GTT'AAC Cuts at: 0 831 3135 Size: 831 2304 MfeI C'AATT_G Cuts at: 0 544 3135 Size: 544 2591 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 3067 3135 Size: 3067 68 MscI TGG'CCA Cuts at: 0 480 3135 Size: 480 2655 MslI CAynn'nnrTG Cuts at: 0 640 1402 1567 2545 2833 3135 Size: 640 762 165 978 288 302 Fragments arranged by size: 978 762 640 302 288 165 MspA1I CmG'CkG Cuts at: 0 870 2973 3091 3135 Size: 870 2103 118 44 Fragments arranged by size: 2103 870 118 44 NaeI GCC'GGC Cuts at: 0 33 3135 Size: 33 3102 NcoI C'CATG_G Cuts at: 0 998 1562 3135 Size: 998 564 1573 Fragments arranged by size: 1573 998 564 NdeI CA'TA_TG Cuts at: 0 1403 3135 Size: 1403 1732 NgoMIV G'CCGG_C Cuts at: 0 31 3135 Size: 31 3104 NheI G'CTAG_C Cuts at: 0 816 3135 Size: 816 2319 NsiI A_TGCA'T Cuts at: 0 380 3135 Size: 380 2755 NspI r_CATG'y Cuts at: 0 512 1194 1332 1762 2215 2386 3135 Size: 512 682 138 430 453 171 749 Fragments arranged by size: 749 682 512 453 430 171 138 PciI A'CATG_T Cuts at: 0 508 1190 3135 Size: 508 682 1945 Fragments arranged by size: 1945 682 508 PflMI CCAn_nnn'nTGG Cuts at: 0 458 3135 Size: 458 2677 PfoI T'CCnGG_A Cuts at: 0 788 1322 3135 Size: 788 534 1813 Fragments arranged by size: 1813 788 534 PpuMI rG'GwC_Cy Cuts at: 0 105 3135 Size: 105 3030 PspOMI G'GGCC_C Cuts at: 0 2707 3135 Size: 2707 428 PspXI vC'TCGA_Gb Cuts at: 0 54 3135 Size: 54 3081 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1649 1681 3135 Size: 1649 32 1454 Fragments arranged by size: 1649 1454 32 PstI C_TGCA'G Cuts at: 0 17 396 711 1268 2972 3018 3135 Size: 17 379 315 557 1704 46 117 Fragments arranged by size: 1704 557 379 315 117 46 17 PvuII CAG'CTG Cuts at: 0 870 2973 3091 3135 Size: 870 2103 118 44 Fragments arranged by size: 2103 870 118 44 SapI GCTCTTCn'nnn_ Cuts at: 0 1485 2084 2269 3135 Size: 1485 599 185 866 Fragments arranged by size: 1485 866 599 185 ScaI AGT'ACT Cuts at: 0 1695 3135 Size: 1695 1440 SexAI A'CCwGG_T Cuts at: 0 2663 3135 Size: 2663 472 SfcI C'TryA_G Cuts at: 0 13 215 392 707 1264 2968 3014 3135 Size: 13 202 177 315 557 1704 46 121 Fragments arranged by size: 1704 557 315 202 177 121 46 13 SmlI C'TyrA_G Cuts at: 0 54 1101 1840 2029 2507 3135 Size: 54 1047 739 189 478 628 Fragments arranged by size: 1047 739 628 478 189 54 SphI G_CATG'C Cuts at: 0 1762 2215 3135 Size: 1762 453 920 Fragments arranged by size: 1762 920 453 StuI AGG'CCT Cuts at: 0 1907 2618 3135 Size: 1907 711 517 Fragments arranged by size: 1907 711 517 StyI C'CwwG_G Cuts at: 0 998 1125 1454 1562 2891 2943 2979 3135 Size: 998 127 329 108 1329 52 36 156 Fragments arranged by size: 1329 998 329 156 127 108 52 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1544 2430 3135 Size: 1544 886 705 Fragments arranged by size: 1544 886 705 TatI w'GTAC_w Cuts at: 0 594 1193 1357 1462 1468 1693 1718 1844 Size: 594 599 164 105 6 225 25 126 Cuts at: 1844 1937 2555 3135 Size: 93 618 580 Fragments arranged by size: 618 599 594 580 225 164 126 105 93 25 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 770 802 3135 Size: 770 32 2333 Fragments arranged by size: 2333 770 32 XbaI T'CTAG_A Cuts at: 0 3003 3135 Size: 3003 132 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2898 3135 Size: 2898 237 XhoI C'TCGA_G Cuts at: 0 54 3135 Size: 54 3081 XmnI GAAnn'nnTTC Cuts at: 0 899 3135 Size: 899 2236 Enzymes that do cut: AccI AcuI AflIII AlfI AloI ApaI ApaLI ApoI AseI AvaI AvrII BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI BstYI BtgI BtsI DraI DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HpaI MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NsiI NspI PciI PflMI PfoI PpuMI PspOMI PspXI PsrI PstI PvuII SapI ScaI SexAI SfcI SmlI SphI StuI StyI TaqII TatI TstI XbaI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlwNI AscI AsiSI BaeI BamHI BbeI BbvCI BlpI BmgBI BplI BsaAI BsaHI BsaWI BsiWI BsmBI BspEI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EciI EcoICRI EcoRV FseI FspI FspAI HaeII HindIII KasI KpnI MluI NarI NotI NruI PacI PasI PmeI PmlI PpiI PshAI PsiI PvuI RsrII SacI SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XmaI ZraI