################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BcnI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AciI 955 GCGG CCGC(-3/-1) AfaI 772 GTAC GT^AC AflIII 12 ACATGT A^C[GA][CT]GT BalI 41 TGGCCA TGG^CCA BanII 913 GAGCTC G[GA]GC[CT]^C BbvCI 1339 GCTGAGG CCTCAGC(-5/-2) BciVI 1281 GGATAC GTATCC(6/5) BclI 663 TGATCA T^GATCA BfaI 109 CTAG C^TAG BlpI 1605 GCTGAGC GC^T[ACGT]AGC BpmI 575 CTGGAG CTGGAG(16/14) Bpu1102I 1605 GCTGAGC GC^T[ACGT]AGC BsmFI 1647 GGGAC GGGAC(10/14) BspLU11I 12 ACATGT A^CATGT CelII 1605 GCTGAGC GC^T[ACGT]AGC DraI 1114 TTTAAA TTT^AAA EaeI 41 TGGCCA [CT]^GGCC[GA] EcoRI 531 GAATTC G^AATTC EcoT14I 140 CCAAGG C^C[AT][AT]GG EcoT22I 688 ATGCAT ATGCA^T FbaI 663 TGATCA T^GATCA MaeI 109 CTAG C^TAG MaeIII 1538 GTGAC ^GT[ACGT]AC MfeI 842 CAATTG C^AATTG MluNI 41 TGGCCA TGG^CCA MscI 41 TGGCCA TGG^CCA MunI 842 CAATTG C^AATTG NlaIV 1647 GGGACC GG[ACGT]^[ACGT]CC NsiI 688 ATGCAT ATGCA^T PflMI 44 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 629 CTGCAG CTGCA^G RsaI 772 GTAC GT^AC SacI 913 GAGCTC GAGCT^C StyI 140 CCAAGG C^C[AT][AT]GG Tsp45I 1538 GTGAC ^GT[GC]AC Van91I 44 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 108 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AspHI 913 GAGCTC G[AT]GC[AT]^C 1616 GTGCTC AvaII 541 GGTCC G^G[AT]CC 1648 GGACC BbsI 497 GAAGAC GAAGAC(2/6) 682 GAAGAC BmyI 913 GAGCTC G[AGT]GC[ACT]^C 1616 GTGCTC BpuAI 497 GAAGAC GAAGAC(2/6) 682 GAAGAC BseRI 885 GAGGAG GAGGAG(10/8) 1022 GAGGAG BsiHKAI 913 GAGCTC G[AT]GC[AT]^C 1616 GTGCTC BsmAI 1183 GAGAC GTCTC(1/5) 1432 GAGAC BsmI 366 GAATGC GAATGC(1/-1) 1499 GAATGC Bsp1286I 913 GAGCTC G[AGT]GC[ACT]^C 1616 GTGCTC BspHI 342 TCATGA T^CATGA 1275 TCATGA BssKI 271 CCAGG ^CC[ACGT]GG 1053 CCAGG BstBI 158 TTCGAA TT^CGAA 191 TTCGAA BstF5I 1187 CATCC GGATG(2/0) 1245 GGATG BstNI 271 CCAGG CC^[AT]GG 1053 CCAGG BstYI 900 AGATCC [GA]^GATC[CT] 1258 GGATCT Cac8I 54 GCTTGC GC[ACGT]^[ACGT]GC 1593 GCTGGC EarI 817 GAAGAG CTCTTC(1/4) 1014 GAAGAG EcoRII 271 CCAGG ^CC[AT]GG 1053 CCAGG FokI 1187 CATCC GGATG(9/13) 1245 GGATG HincII 280 GTTGAC GT[CT]^[GA]AC 1041 GTCAAC HindII 280 GTTGAC GT[CT]^[GA]AC 1041 GTCAAC HphI 144 GGTGA GGTGA(8/7) 136 TCACC Ksp632I 817 GAAGAG CTCTTC(1/4) 1014 GAAGAG MflI 900 AGATCC [GA]^GATC[CT] 1258 GGATCT MslI 9 CACACATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 960 CACACTCATG MspA1I 1553 CAGCTG C[AC]G^C[GT]G 1591 CAGCTG MvaI 271 CCAGG CC^[AT]GG 1053 CCAGG MwoI 714 GCCTTCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1618 GCTCCCTATGC NspI 12 ACATGT [GA]CATG^[CT] 686 ACATGC NspV 158 TTCGAA TT^CGAA 191 TTCGAA PleI 1144 GAGTC GAGTC(4/5) 1405 GAGTC PvuII 1553 CAGCTG CAG^CTG 1591 CAGCTG RcaI 342 TCATGA T^CATGA 1275 TCATGA ScrFI 271 CCAGG CC^[ACGT]GG 1053 CCAGG SfaNI 1194 GATGC GCATC(5/9) 307 GCATC SfcI 629 CTGCAG C^T[GA][CT]AG 1527 CTGTAG SfuI 158 TTCGAA TT^CGAA 191 TTCGAA TaqI 159 TCGA T^CGA 192 TCGA TthHB8I 159 TCGA T^CGA 192 TCGA XhoII 900 AGATCC [GA]^GATC[CT] 1258 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AcsI AluI AlwI ApoI Asp700I BbvI BsaJI BsiYI BslI BsrDI BsrI Cfr13I DdeI DpnII Fnu4HI HaeIII HinfI ItaI MboI MboII MnlI MseI NdeII NlaIII Sau3AI Sau96I SspI TfiI Tru9I TseI Tsp509I TspRI XmnI