(Circular) (Six-base) MAPSORT of: pFN21AE9621.seq Check: 5505 from: 1 to: 6373 pFN21AE9621 6373 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4183 278 Size: 53 83 186 936 2647 2468 Fragments arranged by size: 2647 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3716 1937 Size: 1779 4594 Acc65I G'GTAC_C Cuts at: 3694 3694 Size: 6373 AclI AA'CG_TT Cuts at: 1563 4500 4873 1563 Size: 2937 373 3063 Fragments arranged by size: 3063 2937 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2154 2841 3449 4436 5820 6088 1632 Size: 522 687 608 987 1384 268 1917 Fragments arranged by size: 1917 1384 987 687 608 522 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6176 AflIII A'CryG_T Cuts at: 1203 2017 5272 6249 1203 Size: 814 3255 977 1327 Fragments arranged by size: 3255 1327 977 814 AgeI A'CCGG_T Cuts at: 5204 5204 Size: 6373 AhdI GACnn_n'nnGTC Cuts at: 5102 5102 Size: 6373 AleI CACnn'nnGTG Cuts at: 1166 2018 1166 Size: 852 5521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6373 AlwNI CAG_nnn'CTG Cuts at: 4116 5688 4116 Size: 1572 4801 ApaLI G'TGCA_C Cuts at: 4430 5586 4430 Size: 1156 5217 ApoI r'AATT_y Cuts at: 1493 2133 2302 2342 2440 2493 2536 2709 3566 Size: 640 169 40 98 53 43 173 857 Cuts at: 3566 3682 3908 4060 6169 1493 Size: 116 226 152 2109 1697 Fragments arranged by size: 2109 1697 857 640 226 173 169 152 116 98 53 43 40 AseI AT'TA_AT Cuts at: 160 1032 4927 160 Size: 872 3895 1606 Fragments arranged by size: 3895 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6373 AvaI C'yCGr_G Cuts at: 1870 1943 3698 1870 Size: 73 1755 4545 Fragments arranged by size: 4545 1755 73 BamHI G'GATC_C Cuts at: 3703 4070 3703 Size: 367 6006 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3694 5149 618 Size: 325 217 627 1907 1455 1842 Fragments arranged by size: 1907 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2922 3692 1391 Size: 1531 770 4072 Fragments arranged by size: 4072 1531 770 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2509 2694 928 Size: 971 610 185 4607 Fragments arranged by size: 4607 971 610 185 BbvCI CC'TCA_GC Cuts at: 3345 3345 Size: 6373 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4564 4598 1903 Size: 34 2627 34 3678 Fragments arranged by size: 3678 2627 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3280 4265 5475 3280 Size: 985 1210 4178 Fragments arranged by size: 4178 1210 985 BclI T'GATC_A Cuts at: 1548 2668 1548 Size: 1120 5253 BfrBI ATG'CAT Cuts at: 2695 2695 Size: 6373 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4984 136 Size: 107 122 71 631 605 3312 1525 Fragments arranged by size: 3312 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6368 6368 Size: 6373 BlpI GC'TnA_GC Cuts at: 3611 3790 3611 Size: 179 6194 Bme1580I G_kGCm'C Cuts at: 1790 4434 5590 1790 Size: 2644 1156 2573 Fragments arranged by size: 2644 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6373 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2080 4104 5062 Size: 416 53 83 399 24 663 2024 958 Cuts at: 5062 442 Size: 1753 Fragments arranged by size: 2024 1753 958 663 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6373 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2601 5033 1239 Size: 1362 2432 2579 Fragments arranged by size: 2579 2432 1362 Bpu10I CC'TnA_GC Cuts at: 3345 3345 Size: 6373 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4498 5363 5661 4498 Size: 865 298 5210 Fragments arranged by size: 5210 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5036 882 Size: 617 3537 2219 Fragments arranged by size: 3537 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4164 493 Size: 711 2960 2702 Fragments arranged by size: 2960 2702 711 BsaBI GATnn'nnATC Cuts at: 6367 6367 Size: 6373 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4180 4562 5197 275 Size: 53 83 186 936 2647 382 635 1451 Fragments arranged by size: 2647 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4806 5204 5478 5625 6036 4806 Size: 398 274 147 411 5143 Fragments arranged by size: 5143 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6148 Fragments arranged by size: 6148 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2905 3042 1186 Size: 1719 137 4517 Fragments arranged by size: 4517 1719 137 BseYI C'CCAG_C Cuts at: 5576 6072 5576 Size: 496 5877 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5800 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3823 4584 5612 1540 Size: 275 186 1822 761 1028 2301 Fragments arranged by size: 2301 1822 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2922 3625 4434 4519 5590 2922 Size: 703 809 85 1071 3705 Fragments arranged by size: 3705 1071 809 703 85 BsmI GAATG_Cn' Cuts at: 2377 3510 3884 3977 2377 Size: 1133 374 93 4773 Fragments arranged by size: 4773 1133 374 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2922 3625 3692 4434 4519 5590 1391 Size: 399 1132 703 67 742 85 1071 2174 Fragments arranged by size: 2174 1132 1071 742 703 399 85 67 BspEI T'CCGG_A Cuts at: 6036 6036 Size: 6373 BspHI T'CATG_A Cuts at: 2347 3280 4262 5992 2347 Size: 933 982 1730 2728 Fragments arranged by size: 2728 1730 982 933 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3728 844 Size: 2884 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4260 1394 Size: 429 2437 3507 Fragments arranged by size: 3507 2437 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2330 2862 3427 4868 5042 65 Size: 1183 1082 532 565 1441 174 1396 Fragments arranged by size: 1441 1396 1183 1082 565 532 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5017 5204 1700 Size: 110 3207 187 2869 Fragments arranged by size: 3207 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6373 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6373 BssSI C'ACGA_G Cuts at: 4433 5445 4433 Size: 1012 5361 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6373 BstBI TT'CG_AA Cuts at: 2164 2197 5177 2164 Size: 33 2980 3360 Fragments arranged by size: 3360 2980 33 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6373 BstYI r'GATC_y Cuts at: 1972 2905 3263 3703 4070 4455 4472 5924 6010 Size: 933 358 440 367 385 17 1452 86 Cuts at: 6010 6022 6368 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 933 440 385 367 358 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5821 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5634 Fragments arranged by size: 5634 683 56 BtsI GCAGTG_nn' Cuts at: 2245 3889 4683 4703 2245 Size: 1644 794 20 3915 Fragments arranged by size: 3915 1644 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6373 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6338 DraI TTT'AAA Cuts at: 3121 3677 3121 Size: 556 5817 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5380 809 Size: 507 4064 1802 Fragments arranged by size: 4064 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 3820 4709 Size: 54 1425 50 275 18 216 1774 889 Cuts at: 4709 6338 8 Size: 1629 43 Fragments arranged by size: 1774 1629 1425 889 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3820 1537 Size: 275 2008 4090 Fragments arranged by size: 4090 2008 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2817 3014 4303 1353 Size: 1464 197 1289 3423 Fragments arranged by size: 3423 1464 1289 197 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4960 5334 5480 387 Size: 1064 138 3371 374 146 1280 Fragments arranged by size: 3371 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2920 2920 Size: 6373 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2601 2841 3449 4436 5033 5820 Size: 393 522 447 240 608 987 597 787 Cuts at: 5820 6088 1239 Size: 268 1524 Fragments arranged by size: 1524 987 787 608 597 522 447 393 268 240 EcoRI G'AATT_C Cuts at: 2536 3682 4060 2536 Size: 1146 378 4849 Fragments arranged by size: 4849 1146 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2267 2267 Size: 6373 FspI TGC'GCA Cuts at: 4879 4879 Size: 6373 HaeII r_GCGC'y Cuts at: 1678 5520 1678 Size: 3842 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2638 2670 3338 3370 3460 Size: 32 135 32 1066 32 668 32 90 Cuts at: 3460 3492 5014 5046 5088 5120 1373 Size: 32 1522 32 42 32 2626 Fragments arranged by size: 2626 1522 1066 668 135 90 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2287 3048 3717 3963 669 Size: 988 281 349 761 669 246 3079 Fragments arranged by size: 3079 988 761 669 349 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6373 HpaI GTT'AAC Cuts at: 3963 3963 Size: 6373 KpnI G_GTAC'C Cuts at: 3698 3698 Size: 6373 MfeI C'AATT_G Cuts at: 2847 3972 2847 Size: 1125 5248 MluI A'CGCG_T Cuts at: 6249 6249 Size: 6373 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5487 5671 1305 Size: 4182 184 2007 Fragments arranged by size: 4182 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 10 Size: 54 1425 343 216 4335 Fragments arranged by size: 4335 1425 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2018 2969 4331 4690 4849 518 Size: 648 852 951 1362 359 159 2042 Fragments arranged by size: 2042 1362 951 852 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3560 3598 3789 4466 5614 5859 1606 Size: 1954 38 191 677 1148 245 2120 Fragments arranged by size: 2120 1954 1148 677 245 191 38 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5821 NdeI CA'TA_TG Cuts at: 387 387 Size: 6373 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6373 NotI GC'GGCC_GC Cuts at: 3820 3820 Size: 6373 NsiI A_TGCA'T Cuts at: 2697 2697 Size: 6373 NspI r_CATG'y Cuts at: 1132 1229 2021 2695 3731 5276 1132 Size: 97 792 674 1036 1545 2229 Fragments arranged by size: 2229 1545 1036 792 674 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6373 PciI A'CATG_T Cuts at: 2017 5272 2017 Size: 3255 3118 PflMI CCAn_nnn'nTGG Cuts at: 1108 2055 1108 Size: 947 5426 PmeI GTTT'AAAC Cuts at: 3677 3677 Size: 6373 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4442 4474 5955 5987 4442 Size: 32 1481 32 4828 Fragments arranged by size: 4828 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6373 PsiI TTA'TAA Cuts at: 3666 3943 3666 Size: 277 6096 PstI C_TGCA'G Cuts at: 830 2638 3725 830 Size: 1808 1087 3478 Fragments arranged by size: 3478 1808 1087 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6373 PvuII CAG'CTG Cuts at: 3560 3598 3560 Size: 38 6335 SacI G_AGCT'C Cuts at: 2922 2922 Size: 6373 SalI G'TCGA_C Cuts at: 1936 3715 1936 Size: 1779 4594 SbfI CC_TGCA'GG Cuts at: 3725 3725 Size: 6373 ScaI AGT'ACT Cuts at: 4621 6333 4621 Size: 1712 4661 SfcI C'TryA_G Cuts at: 826 1045 2634 3532 3721 4856 5537 5728 826 Size: 219 1589 898 189 1135 681 191 1471 Fragments arranged by size: 1589 1471 1135 898 681 219 191 189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6373 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6373 SmaI CCC'GGG Cuts at: 1872 3700 1872 Size: 1828 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4477 5378 5640 820 Size: 197 926 2534 901 262 1553 Fragments arranged by size: 2534 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4164 493 Size: 3671 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6373 SphI G_CATG'C Cuts at: 1132 3731 1132 Size: 2599 3774 SspI AAT'ATT Cuts at: 5 52 2808 3301 3433 4297 5 Size: 47 2756 493 132 864 2081 Fragments arranged by size: 2756 2081 864 493 132 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6373 StyI C'CwwG_G Cuts at: 513 1065 2145 3812 513 Size: 552 1080 1667 3074 Fragments arranged by size: 3074 1667 1080 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2158 2375 3561 4400 4417 4570 4755 Size: 1255 578 217 1186 839 17 153 185 Cuts at: 4755 5200 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1186 839 578 445 217 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4619 6331 96 Size: 274 80 33 51 1443 2642 1712 138 Fragments arranged by size: 2642 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6333 6365 6333 Size: 32 6341 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6373 XbaI T'CTAG_A Cuts at: 2113 3709 2113 Size: 1596 4777 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6373 XmaI C'CCGG_G Cuts at: 1870 3698 1870 Size: 1828 4545 XmnI GAAnn'nnTTC Cuts at: 2133 2247 2343 4502 2133 Size: 114 96 2159 4004 Fragments arranged by size: 4004 2159 114 96 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4181 276 Size: 53 83 186 936 2647 2468 Fragments arranged by size: 2647 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI