(Circular) (Six-base) MAPSORT of: pFN21AE9516.seq Check: 7362 from: 1 to: 6931 pFN21AE9516 6931 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3305 3483 3305 Size: 178 6753 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4741 278 Size: 53 83 186 936 3205 2468 Fragments arranged by size: 3205 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4274 1937 Size: 2337 4594 Acc65I G'GTAC_C Cuts at: 4252 4252 Size: 6931 AclI AA'CG_TT Cuts at: 1563 5058 5431 1563 Size: 3495 373 3063 Fragments arranged by size: 3495 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4994 6378 6646 1632 Size: 3362 1384 268 1917 Fragments arranged by size: 3362 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6734 AflIII A'CryG_T Cuts at: 1203 5830 6807 1203 Size: 4627 977 1327 Fragments arranged by size: 4627 1327 977 AgeI A'CCGG_T Cuts at: 5762 5762 Size: 6931 AhdI GACnn_n'nnGTC Cuts at: 5660 5660 Size: 6931 AleI CACnn'nnGTG Cuts at: 1166 3380 1166 Size: 2214 4717 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6931 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2966 2998 2966 Size: 32 6899 AlwNI CAG_nnn'CTG Cuts at: 2079 2578 3202 3271 3673 3774 4674 6246 2079 Size: 499 624 69 402 101 900 1572 2764 Fragments arranged by size: 2764 1572 900 624 499 402 101 69 ApaI G_GGCC'C Cuts at: 4207 4207 Size: 6931 ApaLI G'TGCA_C Cuts at: 4988 6144 4988 Size: 1156 5775 ApoI r'AATT_y Cuts at: 1493 2237 4160 4240 4466 4618 6727 1493 Size: 744 1923 80 226 152 2109 1697 Fragments arranged by size: 2109 1923 1697 744 226 152 80 AseI AT'TA_AT Cuts at: 160 1032 5485 160 Size: 872 4453 1606 Fragments arranged by size: 4453 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6931 AvaI C'yCGr_G Cuts at: 1870 1943 2287 4200 4256 1870 Size: 73 344 1913 56 4545 Fragments arranged by size: 4545 1913 344 73 56 BamHI G'GATC_C Cuts at: 2742 2902 4261 4628 2742 Size: 160 1359 367 5045 Fragments arranged by size: 5045 1359 367 160 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 2680 2694 4061 4252 Size: 325 217 627 304 589 14 1367 191 Cuts at: 4252 5707 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1367 627 589 325 304 217 191 14 BanII G_rGCy'C Cuts at: 1391 3458 3506 3636 4207 4250 1391 Size: 2067 48 130 571 43 4072 Fragments arranged by size: 4072 2067 571 130 48 43 BbeI G_GCGC'C Cuts at: 2684 4065 2684 Size: 1381 5550 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5960 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2199 2233 5122 5156 1903 Size: 34 262 34 2889 34 3678 Fragments arranged by size: 3678 2889 262 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4823 6033 4823 Size: 1210 5721 BclI T'GATC_A Cuts at: 1548 3372 1548 Size: 1824 5107 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2442 3624 3699 Size: 107 122 71 631 605 770 1182 75 Cuts at: 3699 4059 4211 5542 136 Size: 360 152 1331 1525 Fragments arranged by size: 1525 1331 1182 770 631 605 360 152 122 107 75 71 BglII A'GATC_T Cuts at: 6926 6926 Size: 6931 BlpI GC'TnA_GC Cuts at: 2602 4348 2602 Size: 1746 5185 Bme1580I G_kGCm'C Cuts at: 1790 2086 2096 3246 3714 4207 4992 6148 1790 Size: 296 10 1150 468 493 785 1156 2573 Fragments arranged by size: 2573 1156 1150 785 493 468 296 10 BmgBI CAC'GTC Cuts at: 1318 3178 3349 4176 1318 Size: 1860 171 827 4073 Fragments arranged by size: 4073 1860 827 171 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3256 4662 5620 Size: 416 53 83 399 24 1839 1406 958 Cuts at: 5620 442 Size: 1753 Fragments arranged by size: 1839 1753 1406 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6931 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2383 2383 Size: 6931 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2056 2550 2682 2913 3023 3650 3826 5591 Size: 817 494 132 231 110 627 176 1765 Cuts at: 5591 1239 Size: 2579 Fragments arranged by size: 2579 1765 817 627 494 231 176 132 110 Bpu10I CC'TnA_GC Cuts at: 3314 3314 Size: 6931 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3152 5056 5921 6219 3152 Size: 1904 865 298 3864 Fragments arranged by size: 3864 1904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3153 5594 882 Size: 617 1654 2441 2219 Fragments arranged by size: 2441 2219 1654 617 BsaAI yAC'GTr Cuts at: 493 1204 4722 493 Size: 711 3518 2702 Fragments arranged by size: 3518 2702 711 BsaBI GATnn'nnATC Cuts at: 6925 6925 Size: 6931 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2681 3046 3729 4062 Size: 53 83 186 936 1148 365 683 333 Cuts at: 4062 4738 5120 5755 275 Size: 676 382 635 1451 Fragments arranged by size: 1451 1148 936 683 676 635 382 365 333 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 5364 5762 6036 6183 6594 4116 Size: 1248 398 274 147 411 4453 Fragments arranged by size: 4453 1248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2001 2031 1178 Size: 30 165 30 598 30 6078 Fragments arranged by size: 6078 598 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2095 3020 1186 Size: 909 925 5097 Fragments arranged by size: 5097 925 909 BseYI C'CCAG_C Cuts at: 2709 3271 4139 6134 6630 2709 Size: 562 868 1995 496 3010 Fragments arranged by size: 3010 1995 868 562 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2302 2383 3461 3566 3961 4064 1163 Size: 573 566 81 1078 105 395 103 4030 Fragments arranged by size: 4030 1078 573 566 395 105 103 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3763 4012 4381 5142 6170 1540 Size: 275 186 1762 249 369 761 1028 2301 Fragments arranged by size: 2301 1762 1028 761 369 275 249 186 BsiHKAI G_wGCw'C Cuts at: 3636 4992 5077 6148 3636 Size: 1356 85 1071 4419 Fragments arranged by size: 4419 1356 1071 85 BsmI GAATG_Cn' Cuts at: 2027 2980 4442 4535 2027 Size: 953 1462 93 4423 Fragments arranged by size: 4423 1462 953 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2658 3736 2658 Size: 1078 5853 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2096 3246 3458 3506 3636 3714 Size: 399 296 10 1150 212 48 130 78 Cuts at: 3714 4207 4250 4992 5077 6148 1391 Size: 493 43 742 85 1071 2174 Fragments arranged by size: 2174 1150 1071 742 493 399 296 212 130 85 78 48 43 10 BspEI T'CCGG_A Cuts at: 6594 6594 Size: 6931 BspHI T'CATG_A Cuts at: 2736 4820 6550 2736 Size: 2084 1730 3117 Fragments arranged by size: 3117 2084 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2985 3305 3483 4083 4286 844 Size: 2141 320 178 600 203 3489 Fragments arranged by size: 3489 2141 600 320 203 178 BsrBI CCG'CTC Cuts at: 1394 1823 2824 4818 1394 Size: 429 1001 1994 3507 Fragments arranged by size: 3507 1994 1001 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2951 3850 5426 5600 65 Size: 1183 1703 899 1576 174 1396 Fragments arranged by size: 1703 1576 1396 1183 899 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3521 5575 5762 1700 Size: 110 1711 2054 187 2869 Fragments arranged by size: 2869 2054 1711 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6931 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6931 BssSI C'ACGA_G Cuts at: 2107 4991 6003 2107 Size: 2884 1012 3035 Fragments arranged by size: 3035 2884 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3202 3297 4087 794 Size: 2408 95 790 3638 Fragments arranged by size: 3638 2408 790 95 BstBI TT'CG_AA Cuts at: 2792 5735 2792 Size: 2943 3988 BstEII G'GTnAC_C Cuts at: 1184 2611 1184 Size: 1427 5504 BstXI CCAn_nnnn'nTGG Cuts at: 3517 3517 Size: 6931 BstYI r'GATC_y Cuts at: 1972 2742 2902 4261 4628 5013 5030 6482 6568 Size: 770 160 1359 367 385 17 1452 86 Cuts at: 6568 6580 6926 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1359 770 385 367 346 160 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4208 4208 Size: 6931 BtgI C'CryG_G Cuts at: 513 1065 2004 2412 2916 3028 3191 513 Size: 552 939 408 504 112 163 4253 Fragments arranged by size: 4253 939 552 504 408 163 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3937 487 Size: 683 56 2711 3481 Fragments arranged by size: 3481 2711 683 56 BtsI GCAGTG_nn' Cuts at: 4081 4447 5241 5261 4081 Size: 366 794 20 5751 Fragments arranged by size: 5751 794 366 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6931 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6896 DraI TTT'AAA Cuts at: 4235 4235 Size: 6931 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2837 5938 809 Size: 507 1521 3101 1802 Fragments arranged by size: 3101 1802 1521 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2748 2958 3519 Size: 54 1425 50 275 18 918 210 561 Cuts at: 3519 3541 3570 3597 3760 3948 4186 4378 5267 Size: 22 29 27 163 188 238 192 889 Cuts at: 5267 6896 8 Size: 1629 43 Fragments arranged by size: 1629 1425 918 889 561 275 238 210 192 188 163 54 50 43 29 27 22 18 EagI C'GGCC_G Cuts at: 1537 1812 3760 4378 1537 Size: 275 1948 618 4090 Fragments arranged by size: 4090 1948 618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3151 3215 3407 3500 3968 4861 1353 Size: 1798 64 192 93 468 893 3423 Fragments arranged by size: 3423 1798 893 468 192 93 64 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5518 5892 6038 387 Size: 1064 138 3929 374 146 1280 Fragments arranged by size: 3929 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3634 3634 Size: 6931 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2056 2550 2682 2913 3023 3650 3826 Size: 393 424 494 132 231 110 627 176 Cuts at: 3826 4994 5591 6378 6646 1239 Size: 1168 597 787 268 1524 Fragments arranged by size: 1524 1168 787 627 597 494 424 393 268 231 176 132 110 EcoO109I rG'GnC_Cy Cuts at: 2575 2674 2768 2909 3670 3818 4204 2575 Size: 99 94 141 761 148 386 5302 Fragments arranged by size: 5302 761 386 148 141 99 94 EcoRI G'AATT_C Cuts at: 4160 4240 4618 4160 Size: 80 378 6473 Fragments arranged by size: 6473 378 80 EcoRV GAT'ATC Cuts at: 2734 2734 Size: 6931 FspI TGC'GCA Cuts at: 5437 5437 Size: 6931 HaeII r_GCGC'y Cuts at: 1678 2684 3395 3938 4065 6078 1678 Size: 1006 711 543 127 2013 2531 Fragments arranged by size: 2531 2013 1006 711 543 127 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2001 2033 2448 2480 2641 Size: 32 135 32 429 32 415 32 161 Cuts at: 2641 2673 5572 5604 5646 5678 1373 Size: 32 2899 32 42 32 2626 Fragments arranged by size: 2899 2626 429 415 161 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3061 4275 4521 669 Size: 988 281 1123 1214 246 3079 Fragments arranged by size: 3079 1214 1123 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6931 HpaI GTT'AAC Cuts at: 4521 4521 Size: 6931 KasI G'GCGC_C Cuts at: 2680 4061 2680 Size: 1381 5550 KpnI G_GTAC'C Cuts at: 4256 4256 Size: 6931 MfeI C'AATT_G Cuts at: 4530 4530 Size: 6931 MluI A'CGCG_T Cuts at: 6807 6807 Size: 6931 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3061 3762 6045 6229 1305 Size: 1756 701 2283 184 2007 Fragments arranged by size: 2283 2007 1756 701 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2750 2960 4188 10 Size: 54 1425 343 918 210 1228 2753 Fragments arranged by size: 2753 1425 1228 918 343 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 2382 3380 3857 4889 5248 5407 518 Size: 648 1216 998 477 1032 359 159 2042 Fragments arranged by size: 2042 1216 1032 998 648 477 359 159 MspA1I CmG'CkG Cuts at: 1606 2427 2433 2713 2891 4347 5024 6172 6417 Size: 821 6 280 178 1456 677 1148 245 Cuts at: 6417 1606 Size: 2120 Fragments arranged by size: 2120 1456 1148 821 677 280 245 178 6 NaeI GCC'GGC Cuts at: 3523 3523 Size: 6931 NarI GG'CG_CC Cuts at: 2681 4062 2681 Size: 1381 5550 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5440 Fragments arranged by size: 5440 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6931 NgoMIV G'CCGG_C Cuts at: 3521 3521 Size: 6931 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6931 NotI GC'GGCC_GC Cuts at: 4378 4378 Size: 6931 NspI r_CATG'y Cuts at: 1132 1229 4289 5834 1132 Size: 97 3060 1545 2229 Fragments arranged by size: 3060 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6931 PciI A'CATG_T Cuts at: 5830 5830 Size: 6931 PflMI CCAn_nnn'nTGG Cuts at: 1108 2578 3271 1108 Size: 1470 693 4768 Fragments arranged by size: 4768 1470 693 PfoI T'CCnGG_A Cuts at: 2571 2659 2817 2571 Size: 88 158 6685 Fragments arranged by size: 6685 158 88 PmeI GTTT'AAAC Cuts at: 4235 4235 Size: 6931 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5000 5032 6513 6545 5000 Size: 32 1481 32 5386 Fragments arranged by size: 5386 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2575 3818 2575 Size: 1243 5688 PshAI GACnn'nnGTC Cuts at: 1613 3866 1613 Size: 2253 4678 PsiI TTA'TAA Cuts at: 4501 4501 Size: 6931 PspOMI G'GGCC_C Cuts at: 4203 4203 Size: 6931 PstI C_TGCA'G Cuts at: 830 4059 4283 830 Size: 3229 224 3478 Fragments arranged by size: 3478 3229 224 PvuI CG_AT'CG Cuts at: 2001 4012 2001 Size: 2011 4920 PvuII CAG'CTG Cuts at: 2427 2713 2891 2427 Size: 286 178 6467 Fragments arranged by size: 6467 286 178 SacI G_AGCT'C Cuts at: 3636 3636 Size: 6931 SalI G'TCGA_C Cuts at: 1936 4273 1936 Size: 2337 4594 SapI GCTCTTCn'nnn_ Cuts at: 3968 3968 Size: 6931 SbfI CC_TGCA'GG Cuts at: 4059 4283 4059 Size: 224 6707 ScaI AGT'ACT Cuts at: 4154 5179 6891 4154 Size: 1025 1712 4194 Fragments arranged by size: 4194 1712 1025 SfcI C'TryA_G Cuts at: 826 1045 3745 4055 4279 5414 6095 6286 826 Size: 219 2700 310 224 1135 681 191 1471 Fragments arranged by size: 2700 1471 1135 681 310 224 219 191 SfoI GGC'GCC Cuts at: 2682 4063 2682 Size: 1381 5550 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6931 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6931 SmaI CCC'GGG Cuts at: 1872 2289 4202 4258 1872 Size: 417 1913 56 4545 Fragments arranged by size: 4545 1913 417 56 SmlI C'TyrA_G Cuts at: 820 1017 1943 3131 5035 5936 6198 820 Size: 197 926 1188 1904 901 262 1553 Fragments arranged by size: 1904 1553 1188 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4722 493 Size: 4229 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6931 SphI G_CATG'C Cuts at: 1132 4289 1132 Size: 3157 3774 SspI AAT'ATT Cuts at: 5 52 4855 5 Size: 47 4803 2081 Fragments arranged by size: 4803 2081 47 StuI AGG'CCT Cuts at: 1517 3627 1517 Size: 2110 4821 StyI C'CwwG_G Cuts at: 513 1065 2004 3090 3171 3661 4370 513 Size: 552 939 1086 81 490 709 3074 Fragments arranged by size: 3074 1086 939 709 552 490 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2723 4958 4975 5128 5313 5758 325 Size: 1255 1143 2235 17 153 185 445 1498 Fragments arranged by size: 2235 1498 1255 1143 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4152 5177 6889 Size: 274 80 33 51 1443 2175 1025 1712 Cuts at: 6889 96 Size: 138 Fragments arranged by size: 2175 1712 1443 1025 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3119 3151 6891 6923 3119 Size: 32 3740 32 3127 Fragments arranged by size: 3740 3127 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2486 2567 1316 Size: 1170 81 5680 Fragments arranged by size: 5680 1170 81 XbaI T'CTAG_A Cuts at: 4267 4267 Size: 6931 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3385 3514 3385 Size: 129 6802 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6931 XmaI C'CCGG_G Cuts at: 1870 2287 4200 4256 1870 Size: 417 1913 56 4545 Fragments arranged by size: 4545 1913 417 56 XmnI GAAnn'nnTTC Cuts at: 5060 5060 Size: 6931 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4739 276 Size: 53 83 186 936 3205 2468 Fragments arranged by size: 3205 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoNI FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI