Restriction map for
pFN21AE9619
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI Eam1105I Eco47III EcoO65I
EcoT14I EcoT22I FbaI FseI FspI
HaeII HapII Hin1I HindIII HpaI
HpaII KasI KpnI MamI MfeI
MflI MluI MluNI MroI MscI
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AfaI 473 GTAC GT^AC
AflIII 125 ACGTGT A^C[GA][CT]GT
Alw44I 703 GTGCAC G^TGCAC
AlwI 214 GATCC GGATC(4/5)
AocI 1050 CCTTAGG CC^T[ACGT]AGG
ApaLI 703 GTGCAC G^TGCAC
AspHI 703 GTGCAC G[AT]GC[AT]^C
AvaII 559 GGACC G^G[AT]CC
BfaI 787 CTAG C^TAG
BpmI 296 CTCCAG CTGGAG(16/14)
BsaI 205 GAGACC GGTCTC(1/5)
BseRI 293 CTCCTC GAGGAG(10/8)
BsiEI 22 CGGCCG CG[GA][CT]^CG
BsiHKAI 703 GTGCAC G[AT]GC[AT]^C
BsrI 734 CCAGT ACTGG(1/-1)
BstBI 494 TTCGAA TT^CGAA
Bsu36I 1050 CCTTAGG CC^T[ACGT]AGG
CfoI 47 GCGC GCG^C
DpnII 214 GATC ^GATC
DraII 558 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 22 CGGCCG [CT]^GGCC[GA]
EagI 22 CGGCCG C^GGCCG
EarI 285 CTCTTC CTCTTC(1/4)
EclXI 22 CGGCCG C^GGCCG
Eco52I 22 CGGCCG C^GGCCG
Eco81I 1050 CCTTAGG CC^T[ACGT]AGG
EcoNI 1242 CCTTGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 558 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 256 GAATTC G^AATTC
HgaI 505 GCGTC GACGC(5/10)
HhaI 47 GCGC GCG^C
HinP1I 47 GCGC G^CGC
HincII 894 GTTGAC GT[CT]^[GA]AC
HindII 894 GTTGAC GT[CT]^[GA]AC
HphI 175 GGTGA GGTGA(8/7)
Ksp632I 285 CTCTTC CTCTTC(1/4)
MaeI 787 CTAG C^TAG
MboI 214 GATC ^GATC
MslI 867 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 214 GATC ^GATC
NotI 21 GCGGCCGC GC^GGCCGC
NspV 494 TTCGAA TT^CGAA
PpuMI 558 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 473 GTAC GT^AC
Sau3AI 214 GATC ^GATC
SfaNI 792 GCATC GCATC(5/9)
SfuI 494 TTCGAA TT^CGAA
SspI 754 AATATT AAT^ATT
TspRI 735 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 8 CGCG CG^CG
26 CGCG
AcsI 256 GAATTC [GA]^AATT[CT]
769 GAATTT
ApoI 256 GAATTC [GA]^AATT[CT]
769 GAATTT
BbsI 507 GTCTTC GAAGAC(2/6)
1044 GTCTTC
BmyI 569 GTGCCC G[AGT]GC[ACT]^C
703 GTGCAC
BpuAI 507 GTCTTC GAAGAC(2/6)
1044 GTCTTC
BsiYI 5 CCCCGCGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1242 CCTTGACCAGG
BslI 5 CCCCGCGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1242 CCTTGACCAGG
BsmI 915 GAATGC GAATGC(1/-1)
1056 GAATGC
Bsp1286I 569 GTGCCC G[AGT]GC[ACT]^C
703 GTGCAC
BspHI 483 TCATGA T^CATGA
645 TCATGA
BstF5I 1151 CATCC GGATG(2/0)
972 GGATG
BstUI 8 CGCG CG^CG
26 CGCG
Cac8I 113 GCAAGC GC[ACGT]^[ACGT]GC
167 GCAGGC
DraI 1080 TTTAAA TTT^AAA
1174 TTTAAA
DsaI 7 CCGCGG C^C[GA][CT]GG
25 CCGCGG
EcoRV 639 GATATC GAT^ATC
934 GATATC
FauI 6 CCCGC CCCGC(4/6)
9 GCGGG
FokI 1151 CATCC GGATG(9/13)
972 GGATG
KspI 7 CCGCGG CCGC^GG
25 CCGCGG
MaeIII 541 GTCAC ^GT[ACGT]AC
737 GTGAC
MvnI 8 CGCG CG^CG
26 CGCG
PleI 412 GACTC GAGTC(4/5)
535 GAGTC
PvuII 530 CAGCTG CAG^CTG
997 CAGCTG
RcaI 483 TCATGA T^CATGA
645 TCATGA
SacII 7 CCGCGG CCGC^GG
25 CCGCGG
SmlI 156 CTCAAG C^T[CT][GA]AG
1118 CTCAAG
Tsp45I 541 GTCAC ^GT[GC]AC
737 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI Asp700I BbvI BsaJI
BsmAI BssKI BstNI Cfr13I DdeI
EcoRII Fnu4HI HaeIII HinfI ItaI
MaeII MboII MnlI MseI MspA1I
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TthHB8I XmnI