(Circular) (Six-base) MAPSORT of: pFN21AE9619.seq Check: 3613 from: 1 to: 5959 pFN21AE9619 5959 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2499 4022 5406 5674 1632 Size: 867 1523 1384 268 1917 Fragments arranged by size: 1917 1523 1384 867 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 2130 4858 5835 1203 Size: 927 2728 977 1327 Fragments arranged by size: 2728 1327 977 927 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 3702 5274 3702 Size: 1572 4387 ApaLI G'TGCA_C Cuts at: 2708 4016 5172 2708 Size: 1308 1156 3495 Fragments arranged by size: 3495 1308 1156 ApoI r'AATT_y Cuts at: 1493 2261 2774 3268 3494 3646 5755 1493 Size: 768 513 494 226 152 2109 1697 Fragments arranged by size: 2109 1697 768 513 494 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 3284 1870 Size: 73 1341 4545 Fragments arranged by size: 4545 1341 73 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3280 4735 618 Size: 325 217 627 1493 1455 1842 Fragments arranged by size: 1842 1493 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3278 1391 Size: 1887 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2505 3042 928 Size: 971 606 537 3845 Fragments arranged by size: 3845 971 606 537 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4150 4184 1903 Size: 34 2213 34 3678 Fragments arranged by size: 3678 2213 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4570 136 Size: 107 122 71 631 605 2898 1525 Fragments arranged by size: 2898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 2578 2712 4020 5176 1790 Size: 788 134 1308 1156 2573 Fragments arranged by size: 2573 1308 1156 788 134 BmgBI CAC'GTC Cuts at: 1318 2131 1318 Size: 813 5146 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3690 4648 442 Size: 416 53 83 399 24 2273 958 1753 Fragments arranged by size: 2273 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2286 4619 1239 Size: 1047 2333 2579 Fragments arranged by size: 2579 2333 1047 Bpu10I CC'TnA_GC Cuts at: 2399 2399 Size: 5959 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2146 3108 4084 4949 5247 2146 Size: 962 976 865 298 2858 Fragments arranged by size: 2858 976 962 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2204 4622 882 Size: 617 705 2418 2219 Fragments arranged by size: 2418 2219 705 617 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3766 4148 4783 275 Size: 53 83 186 936 2233 382 635 1451 Fragments arranged by size: 2233 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5734 Fragments arranged by size: 5734 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2289 1186 Size: 1103 4856 BseYI C'CCAG_C Cuts at: 2176 2577 5162 5658 2176 Size: 401 2585 496 2477 Fragments arranged by size: 2585 2477 496 401 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5386 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2030 3409 4170 5198 1540 Size: 275 186 29 1379 761 1028 2301 Fragments arranged by size: 2301 1379 1028 761 275 186 29 BsiHKAI G_wGCw'C Cuts at: 2712 4020 4105 5176 2712 Size: 1308 85 1071 3495 Fragments arranged by size: 3495 1308 1071 85 BsmI GAATG_Cn' Cuts at: 2926 3067 3470 3563 2926 Size: 141 403 93 5322 Fragments arranged by size: 5322 403 141 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2578 2712 3278 4020 4105 5176 1391 Size: 399 788 134 566 742 85 1071 2174 Fragments arranged by size: 2174 1071 788 742 566 399 134 85 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 2488 2650 3848 5578 2488 Size: 162 1198 1730 2869 Fragments arranged by size: 2869 1730 1198 162 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3314 844 Size: 2470 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4454 4628 65 Size: 1183 3206 174 1396 Fragments arranged by size: 3206 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4603 4790 1700 Size: 110 2793 187 2869 Fragments arranged by size: 2869 2793 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 2500 4763 2500 Size: 2263 3696 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 3289 3656 4041 4058 5510 5596 5608 5954 Size: 1317 367 385 17 1452 86 12 346 Cuts at: 5954 1972 Size: 1977 Fragments arranged by size: 1977 1452 1317 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3056 3056 Size: 5959 BtgI C'CryG_G Cuts at: 513 1065 2004 2012 2030 513 Size: 552 939 8 18 4442 Fragments arranged by size: 4442 939 552 18 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2821 487 Size: 683 56 1595 3625 Fragments arranged by size: 3625 1595 683 56 BtsI GCAGTG_nn' Cuts at: 3475 4269 4289 3475 Size: 794 20 5145 Fragments arranged by size: 5145 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2257 2292 529 Size: 35 1693 35 4196 Fragments arranged by size: 4196 1693 35 35 DraI TTT'AAA Cuts at: 3087 3181 3263 3087 Size: 94 82 5783 Fragments arranged by size: 5783 94 82 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2027 3406 4295 Size: 54 1425 50 275 18 197 1379 889 Cuts at: 4295 5924 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1379 889 275 197 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2027 3406 1537 Size: 275 215 1379 4090 Fragments arranged by size: 4090 1379 275 215 EarI CTCTTCn'nnn_ Cuts at: 1353 2296 3889 1353 Size: 943 1593 3423 Fragments arranged by size: 3423 1593 943 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2499 4022 4619 5406 5674 1239 Size: 393 654 213 1523 597 787 268 1524 Fragments arranged by size: 1524 1523 787 654 597 393 268 213 EcoNI CCTnn'n_nnAGG Cuts at: 3251 3251 Size: 5959 EcoO109I rG'GnC_Cy Cuts at: 2564 2564 Size: 5959 EcoRI G'AATT_C Cuts at: 2261 3268 3646 2261 Size: 1007 378 4574 Fragments arranged by size: 4574 1007 378 EcoRV GAT'ATC Cuts at: 2646 2941 2646 Size: 295 5664 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 5106 1678 Size: 3428 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2203 2235 2271 2303 3032 Size: 32 135 32 631 32 36 32 729 Cuts at: 3032 3064 4600 4632 4674 4706 1373 Size: 32 1536 32 42 32 2626 Fragments arranged by size: 2626 1536 729 631 135 42 36 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2901 3303 3549 669 Size: 988 281 963 402 246 3079 Fragments arranged by size: 3079 988 963 402 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5073 5257 1305 Size: 3768 184 2007 Fragments arranged by size: 3768 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4137 Fragments arranged by size: 4137 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2876 3917 4276 4435 518 Size: 648 1710 1041 359 159 2042 Fragments arranged by size: 2042 1710 1041 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2032 2537 3004 3375 4052 5200 5445 Size: 408 18 505 467 371 677 1148 245 Cuts at: 5445 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 505 467 408 371 245 18 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4468 Fragments arranged by size: 4468 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 2027 3406 2027 Size: 1379 4580 NspI r_CATG'y Cuts at: 1132 1229 3317 4862 1132 Size: 97 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PfoI T'CCnGG_A Cuts at: 2302 2973 2302 Size: 671 5288 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2564 2564 Size: 5959 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 3311 830 Size: 2481 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 2537 3004 2537 Size: 467 5492 SacII CC_GC'GG Cuts at: 2015 2033 2015 Size: 18 5941 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 3307 4442 5123 5314 826 Size: 219 2262 1135 681 191 1471 Fragments arranged by size: 2262 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2161 3123 4063 4964 5226 820 Size: 197 926 218 962 940 901 262 1553 Fragments arranged by size: 1553 962 940 926 901 262 218 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 2761 3883 5 Size: 47 2709 1122 2081 Fragments arranged by size: 2709 2081 1122 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2004 3398 513 Size: 552 939 1394 3074 Fragments arranged by size: 3074 1394 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2168 3986 4003 4156 4341 4786 325 Size: 1255 588 1818 17 153 185 445 1498 Fragments arranged by size: 1818 1498 1255 588 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2477 4205 5917 Size: 274 80 33 51 1443 500 1728 1712 Cuts at: 5917 96 Size: 138 Fragments arranged by size: 1728 1712 1443 500 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5919 5951 5919 Size: 32 5927 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 2086 2985 3049 4088 2086 Size: 899 64 1039 3957 Fragments arranged by size: 3957 1039 899 64 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI