Restriction map for
pFN21AE9619
(Linear) (Six-base) MAPSORT of: KIEE9619.seq Check: 2248 from: 1 to: 1254
KIEE9619.orf
With 182 enzymes: SgfI *
July 3, 2012 19:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 1254
Size: 494 760
AflIII A'CryG_T
Cuts at: 0 125 1254
Size: 125 1129
ApaLI G'TGCA_C
Cuts at: 0 703 1254
Size: 703 551
ApoI r'AATT_y
Cuts at: 0 256 769 1254
Size: 256 513 485
Fragments arranged by size:
513 485 256
BbsI GAAGACnn'nnnn_
Cuts at: 0 500 1037 1254
Size: 500 537 217
Fragments arranged by size:
537 500 217
Bme1580I G_kGCm'C
Cuts at: 0 573 707 1254
Size: 573 134 547
Fragments arranged by size:
573 547 134
BmgBI CAC'GTC
Cuts at: 0 126 1254
Size: 126 1128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1254
Size: 281 973
Bpu10I CC'TnA_GC
Cuts at: 0 394 1254
Size: 394 860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 1103 1254
Size: 141 962 151
Fragments arranged by size:
962 151 141
BsaI GGTCTCn'nnnn_
Cuts at: 0 199 1254
Size: 199 1055
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 284 1254
Size: 284 970
BseYI C'CCAG_C
Cuts at: 0 171 572 1254
Size: 171 401 682
Fragments arranged by size:
682 401 171
BsiEI CG_ry'CG
Cuts at: 0 25 1254
Size: 25 1229
BsiHKAI G_wGCw'C
Cuts at: 0 707 1254
Size: 707 547
BsmI GAATG_Cn'
Cuts at: 0 921 1062 1254
Size: 921 141 192
Fragments arranged by size:
921 192 141
Bsp1286I G_dGCh'C
Cuts at: 0 573 707 1254
Size: 573 134 547
Fragments arranged by size:
573 547 134
BspHI T'CATG_A
Cuts at: 0 483 645 1254
Size: 483 162 609
Fragments arranged by size:
609 483 162
BstBI TT'CG_AA
Cuts at: 0 495 1254
Size: 495 759
Bsu36I CC'TnA_GG
Cuts at: 0 1051 1254
Size: 1051 203
BtgI C'CryG_G
Cuts at: 0 7 25 1254
Size: 7 18 1229
Fragments arranged by size:
1229 18 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 816 1254
Size: 816 438
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 252 287 1254
Size: 252 35 967
Fragments arranged by size:
967 252 35
DraI TTT'AAA
Cuts at: 0 1082 1176 1254
Size: 1082 94 78
Fragments arranged by size:
1082 94 78
EaeI y'GGCC_r
Cuts at: 0 22 1254
Size: 22 1232
EagI C'GGCC_G
Cuts at: 0 22 1254
Size: 22 1232
EarI CTCTTCn'nnn_
Cuts at: 0 291 1254
Size: 291 963
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 494 1254
Size: 281 213 760
Fragments arranged by size:
760 281 213
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1246 1254
Size: 1246 8
EcoO109I rG'GnC_Cy
Cuts at: 0 559 1254
Size: 559 695
EcoRI G'AATT_C
Cuts at: 0 256 1254
Size: 256 998
EcoRV GAT'ATC
Cuts at: 0 641 936 1254
Size: 641 295 318
Fragments arranged by size:
641 318 295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 198 230 266 298 1027 1059 1254
Size: 198 32 36 32 729 32 195
Fragments arranged by size:
729 198 195 36 32 32 32
HincII GTy'rAC
Cuts at: 0 896 1254
Size: 896 358
MslI CAynn'nnrTG
Cuts at: 0 871 1254
Size: 871 383
MspA1I CmG'CkG
Cuts at: 0 9 27 532 999 1254
Size: 9 18 505 467 255
Fragments arranged by size:
505 467 255 18 9
NotI GC'GGCC_GC
Cuts at: 0 22 1254
Size: 22 1232
PfoI T'CCnGG_A
Cuts at: 0 297 968 1254
Size: 297 671 286
Fragments arranged by size:
671 297 286
PpuMI rG'GwC_Cy
Cuts at: 0 559 1254
Size: 559 695
PvuII CAG'CTG
Cuts at: 0 532 999 1254
Size: 532 467 255
Fragments arranged by size:
532 467 255
SacII CC_GC'GG
Cuts at: 0 10 28 1254
Size: 10 18 1226
Fragments arranged by size:
1226 18 10
SmlI C'TyrA_G
Cuts at: 0 156 1118 1254
Size: 156 962 136
Fragments arranged by size:
962 156 136
SspI AAT'ATT
Cuts at: 0 756 1254
Size: 756 498
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 1254
Size: 163 1091
TatI w'GTAC_w
Cuts at: 0 472 1254
Size: 472 782
XmnI GAAnn'nnTTC
Cuts at: 0 81 980 1044 1254
Size: 81 899 64 210
Fragments arranged by size:
899 210 81 64
Enzymes that do cut:
AcuI AflIII ApaLI ApoI BbsI Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BstBI Bsu36I BtgI BtgZI CspCI DraI
EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV
Hin4I HincII MslI MspA1I NotI PfoI PpuMI PvuII
SacII SmlI SspI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I
BtsI ClaI DraIII DrdI EciI EcoICRI FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI StyI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI