Restriction map for
pFN21AE3355
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# NO CUT ENZYMES ( 68 enzymes) #
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Acc65I AccIII AclI AflII AhdI
Alw44I AocI ApaLI AscI Asp700I
Asp718I AspEI AvrII BciVI BfrI
BlnI BsaBI BseAI BsiEI BsiWI
BsmI BspDI BspEI BsrDI BssHII
BstAPI BstEII BstPI Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoT22I
HindIII HpaI KpnI KspI MamI
MluI MroI NotI NruI NsiI
PacI PaeR7I PmeI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
ScaI SgrAI SnaBI SpeI SspI
SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 2116 GACGTC GACGT^C
AccII 65 CGCG CG^CG
AcsI 922 GAATTC [GA]^AATT[CT]
AgeI 1618 ACCGGT A^CCGGT
AlwNI 1687 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 922 GAATTC [GA]^AATT[CT]
AseI 1870 ATTAAT AT^TAAT
AsnI 1870 ATTAAT AT^TAAT
AspI 1171 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2408 TGCGCA TGC^GCA
BbrPI 800 CACGTG CAC^GTG
BcgI 822 GCAGGACAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 884 TGATCA T^GATCA
BglII 1604 AGATCT A^GATCT
BsaI 1193 GAGACC GGTCTC(1/5)
BsmBI 2716 GAGACG CGTCTC(1/5)
Bsp1407I 2553 TGTACA T^GTACA
BspHI 1580 TCATGA T^CATGA
BspLU11I 1653 ACATGT A^CATGT
BsrGI 2553 TGTACA T^GTACA
BssSI 1799 CTCGTG CACGAG(-5/-1)
Bst1107I 2667 GTATAC GTA^TAC
BstBI 1085 TTCGAA TT^CGAA
BstUI 65 CGCG CG^CG
BstZ17I 2667 GTATAC GTA^TAC
DraIII 598 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2275 GAAGAG CTCTTC(1/4)
EcoRI 922 GAATTC G^AATTC
EcoRV 1115 GATATC GAT^ATC
FbaI 884 TGATCA T^GATCA
FseI 811 GGCCGGCC GGCCGG^CC
FspI 2408 TGCGCA TGC^GCA
HincII 2768 GTCAAC GT[CT]^[GA]AC
HindII 2768 GTCAAC GT[CT]^[GA]AC
Ksp632I 2275 GAAGAG CTCTTC(1/4)
MvnI 65 CGCG CG^CG
NaeI 812 GCCGGC GCC^GGC
NdeI 1708 CATATG CA^TATG
NgoMIV 812 GCCGGC G^CCGGC
NheI 1462 GCTAGC G^CTAGC
NspV 1085 TTCGAA TT^CGAA
PflFI 1171 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1618 ACCGGT A^CCGGT
PmaCI 800 CACGTG CAC^GTG
PmlI 800 CACGTG CAC^GTG
PshBI 1870 ATTAAT AT^TAAT
RcaI 1580 TCATGA T^CATGA
SapI 2275 GAAGAGC GCTCTTC(1/4)
SexAI 599 ACCAGGT A^CC[AT]GGT
SfiI 2749 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1085 TTCGAA TT^CGAA
SphI 2102 GCATGC GCATG^C
SspBI 2553 TGTACA T^GTACA
Tth111I 1171 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1821 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccI 2667 GTATAC GT^[AC][GT]AC
2704 GTCTAC
AflIII 799 ACACGT A^C[GA][CT]GT
1653 ACATGT
Aor51HI 247 AGCGCT AGC^GCT
2985 AGCGCT
ApaI 624 GGGCCC GGGCC^C
975 GGGCCC
AspHI 1969 GTGCTC G[AT]GC[AT]^C
2628 GAGCAC
BalI 124 TGGCCA TGG^CCA
1994 TGGCCA
BamHI 1019 GGATCC G^GATCC
1561 GGATCC
BbsI 955 GAAGAC GAAGAC(2/6)
751 GTCTTC
BglI 316 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2750 GCCTCCCAGGC
BlpI 243 GCTGAGC GC^T[ACGT]AGC
2320 GCTGAGC
Bpu1102I 243 GCTGAGC GC^T[ACGT]AGC
2320 GCTGAGC
BpuAI 955 GAAGAC GAAGAC(2/6)
751 GTCTTC
BsaAI 800 CACGTG [CT]AC^GT[GA]
888 CACGTA
BsaWI 1618 ACCGGT [AT]^CCGG[AT]
2258 ACCGGA
BseRI 1292 CTCCTC GAGGAG(10/8)
3003 CTCCTC
BsiHKAI 1969 GTGCTC G[AT]GC[AT]^C
2628 GAGCAC
BsrBI 2323 GAGCGG CCGCTC(-3/-3)
3115 GAGCGG
CelII 243 GCTGAGC GC^T[ACGT]AGC
2320 GCTGAGC
Eco47III 247 AGCGCT AGC^GCT
2985 AGCGCT
MfeI 480 CAATTG C^AATTG
1370 CAATTG
MluNI 124 TGGCCA TGG^CCA
1994 TGGCCA
MscI 124 TGGCCA TGG^CCA
1994 TGGCCA
MunI 480 CAATTG C^AATTG
1370 CAATTG
NcoI 1187 CCATGG C^CATGG
1590 CCATGG
PflMI 2329 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2517 CCATTCAGTGG
PvuII 2318 CAGCTG CAG^CTG
2857 CAGCTG
SbfI 570 CCTGCAGG CCTGCA^GG
2139 CCTGCAGG
SmlI 259 CTCAAG C^T[CT][GA]AG
1933 CTCAAG
Sse8387I 570 CCTGCAGG CCTGCA^GG
2139 CCTGCAGG
Van91I 2329 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2517 CCATTCAGTGG
XcmI 1187 CCATGGGAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1943 CCAACTGTGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaI AvaII BanI BanII BbeI
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI