(Circular) (Six-base) MAPSORT of: pFN21AE3355.seq Check: 1645 from: 1 to: 7846 pFN21AE3355 7846 bp With 182 enzymes: SgfI * August 27, 2018 20:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2036 2036 Size: 7846 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4125 5656 278 Size: 53 83 186 936 2589 1531 2468 Fragments arranged by size: 2589 2468 1531 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4673 4710 5189 1937 Size: 2736 37 479 4594 Fragments arranged by size: 4594 2736 479 37 Acc65I G'GTAC_C Cuts at: 5167 5167 Size: 7846 AclI AA'CG_TT Cuts at: 1563 5973 6346 1563 Size: 4410 373 3063 Fragments arranged by size: 4410 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2702 3060 4515 4579 4940 5015 5909 7293 Size: 1070 358 1455 64 361 75 894 1384 Cuts at: 7293 7561 1632 Size: 268 1917 Fragments arranged by size: 1917 1455 1384 1070 894 361 358 268 75 64 AfeI AGC'GCT Cuts at: 2254 4992 2254 Size: 2738 5108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7649 AflIII A'CryG_T Cuts at: 1203 2804 3658 6745 7722 1203 Size: 1601 854 3087 977 1327 Fragments arranged by size: 3087 1601 1327 977 854 AgeI A'CCGG_T Cuts at: 3623 6677 3623 Size: 3054 4792 AhdI GACnn_n'nnGTC Cuts at: 6575 6575 Size: 7846 AleI CACnn'nnGTG Cuts at: 1166 3531 1166 Size: 2365 5481 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4658 1184 Size: 3474 4372 AlwNI CAG_nnn'CTG Cuts at: 3697 5589 7161 3697 Size: 1892 1572 4382 Fragments arranged by size: 4382 1892 1572 ApaI G_GGCC'C Cuts at: 2633 2984 2633 Size: 351 7495 ApaLI G'TGCA_C Cuts at: 5903 7059 5903 Size: 1156 6690 ApoI r'AATT_y Cuts at: 1493 2927 5155 5381 5533 7642 1493 Size: 1434 2228 226 152 2109 1697 Fragments arranged by size: 2228 2109 1697 1434 226 152 AseI AT'TA_AT Cuts at: 160 1032 3876 6400 160 Size: 872 2844 2524 1606 Fragments arranged by size: 2844 2524 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7846 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2616 2633 2841 2955 3075 3238 Size: 73 120 553 17 208 114 120 163 Cuts at: 3238 5171 1870 Size: 1933 4545 Fragments arranged by size: 4545 1933 553 208 163 120 120 114 73 17 BamHI G'GATC_C Cuts at: 3024 3566 5176 5543 3024 Size: 542 1610 367 5327 Fragments arranged by size: 5327 1610 542 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2222 2242 2349 3071 3755 Size: 325 217 627 435 20 107 722 684 Cuts at: 3755 4385 5167 6622 618 Size: 630 782 1455 1842 Fragments arranged by size: 1842 1455 782 722 684 630 627 435 325 217 107 20 BanII G_rGCy'C Cuts at: 1391 2633 2956 2984 3298 4315 5165 1391 Size: 1242 323 28 314 1017 850 4072 Fragments arranged by size: 4072 1242 1017 850 323 314 28 BbeI G_GCGC'C Cuts at: 2226 2353 3759 2226 Size: 127 1406 6313 Fragments arranged by size: 6313 1406 127 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2749 2967 928 Size: 971 850 218 5807 Fragments arranged by size: 5807 971 850 218 BbvCI CC'TCA_GC Cuts at: 4196 4566 4865 4196 Size: 370 299 7177 Fragments arranged by size: 7177 370 299 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2816 2850 6037 6071 1903 Size: 34 879 34 3187 34 3678 Fragments arranged by size: 3678 3187 879 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5738 6948 5738 Size: 1210 6636 BclI T'GATC_A Cuts at: 1548 2889 1548 Size: 1341 6505 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2327 4761 6457 Size: 107 122 71 631 605 655 2434 1696 Cuts at: 6457 136 Size: 1525 Fragments arranged by size: 2434 1696 1525 655 631 605 122 107 71 BglII A'GATC_T Cuts at: 3609 7841 3609 Size: 4232 3614 BlpI GC'TnA_GC Cuts at: 2249 4326 5263 2249 Size: 2077 937 4832 Fragments arranged by size: 4832 2077 937 Bme1580I G_kGCm'C Cuts at: 1790 2182 2481 2633 2984 3392 5907 7063 1790 Size: 392 299 152 351 408 2515 1156 2573 Fragments arranged by size: 2573 2515 1156 408 392 351 299 152 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7846 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3144 4899 5577 Size: 416 53 83 399 24 1727 1755 678 Cuts at: 5577 6535 442 Size: 958 1753 Fragments arranged by size: 1755 1753 1727 958 678 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3471 1056 Size: 2415 5431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2714 3473 4496 6506 1239 Size: 1475 759 1023 2010 2579 Fragments arranged by size: 2579 2010 1475 1023 759 Bpu10I CC'TnA_GC Cuts at: 2111 4196 4314 4566 4865 2111 Size: 2085 118 252 299 5092 Fragments arranged by size: 5092 2085 299 252 118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2249 3923 5971 6836 7134 2249 Size: 1674 2048 865 298 2961 Fragments arranged by size: 2961 2048 1674 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3192 6509 882 Size: 617 1693 3317 2219 Fragments arranged by size: 3317 2219 1693 617 BsaAI yAC'GTr Cuts at: 493 1204 2807 2895 5637 493 Size: 711 1603 88 2742 2702 Fragments arranged by size: 2742 2702 1603 711 88 BsaBI GATnn'nnATC Cuts at: 7840 7840 Size: 7846 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2223 2350 2562 3756 Size: 53 83 186 936 690 127 212 1194 Cuts at: 3756 4122 5653 6035 6670 275 Size: 366 1531 382 635 1451 Fragments arranged by size: 1531 1451 1194 936 690 635 382 366 212 186 127 83 53 BsaWI w'CCGG_w Cuts at: 3623 4263 6279 6677 6951 7098 7509 3623 Size: 640 2016 398 274 147 411 3960 Fragments arranged by size: 3960 2016 640 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4542 4572 4938 4968 1178 Size: 30 165 30 3139 30 366 30 4056 Fragments arranged by size: 4056 3139 366 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3288 4999 1186 Size: 2102 1711 4033 Fragments arranged by size: 4033 2102 1711 BseYI C'CCAG_C Cuts at: 2449 2977 3489 4321 4904 7049 7545 2449 Size: 528 512 832 583 2145 496 2750 Fragments arranged by size: 2750 2145 832 583 528 512 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2618 2814 3555 1163 Size: 573 882 196 741 5454 Fragments arranged by size: 5454 882 741 573 196 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5296 6057 7085 1540 Size: 275 186 3295 761 1028 2301 Fragments arranged by size: 3295 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3978 4637 5907 5992 7063 3978 Size: 659 1270 85 1071 4761 Fragments arranged by size: 4761 1270 1071 659 85 BsmI GAATG_Cn' Cuts at: 5357 5450 5357 Size: 93 7753 BsmBI CGTCTCn'nnnn_ Cuts at: 4715 4715 Size: 7846 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2182 2481 2633 2956 2984 3298 3392 Size: 399 392 299 152 323 28 314 94 Cuts at: 3392 3978 4315 4637 5165 5907 5992 7063 1391 Size: 586 337 322 528 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 586 528 399 392 337 323 322 314 299 152 94 85 28 BspEI T'CCGG_A Cuts at: 7509 7509 Size: 7846 BspHI T'CATG_A Cuts at: 3585 5735 7465 3585 Size: 2150 1730 3966 Fragments arranged by size: 3966 2150 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2036 2202 2295 3682 4636 4685 5201 844 Size: 1192 166 93 1387 954 49 516 3489 Fragments arranged by size: 3489 1387 1192 954 516 166 93 49 BsrBI CCG'CTC Cuts at: 1394 1823 4330 5122 5733 1394 Size: 429 2507 792 611 3507 Fragments arranged by size: 3507 2507 792 611 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6341 6515 65 Size: 1183 5093 174 1396 Fragments arranged by size: 5093 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2535 2817 3623 6490 6677 1700 Size: 110 725 282 806 2867 187 2869 Fragments arranged by size: 2869 2867 806 725 282 187 110 BsrGI T'GTAC_A Cuts at: 96 4558 96 Size: 4462 3384 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7846 BssSI C'ACGA_G Cuts at: 3804 5906 6918 3804 Size: 2102 1012 4732 Fragments arranged by size: 4732 2102 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7846 BstBI TT'CG_AA Cuts at: 3091 6650 3091 Size: 3559 4287 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7846 BstXI CCAn_nnnn'nTGG Cuts at: 3955 4396 4438 3955 Size: 441 42 7363 Fragments arranged by size: 7363 441 42 BstYI r'GATC_y Cuts at: 1972 2709 3024 3566 3609 4998 5176 5543 5928 Size: 737 315 542 43 1389 178 367 385 Cuts at: 5928 5945 7397 7483 7495 7841 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1389 737 542 385 367 346 315 178 86 43 17 12 BstZ17I GTA'TAC Cuts at: 4674 4674 Size: 7846 BtgI C'CryG_G Cuts at: 513 1065 2004 2132 2187 3192 3595 513 Size: 552 939 128 55 1005 403 4764 Fragments arranged by size: 4764 1005 939 552 403 128 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2986 3909 4318 487 Size: 683 56 1760 923 409 4015 Fragments arranged by size: 4015 1760 923 683 409 56 BtsI GCAGTG_nn' Cuts at: 2180 4171 4959 5362 6156 6176 2180 Size: 1991 788 403 794 20 3850 Fragments arranged by size: 3850 1991 794 788 403 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7846 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4363 4398 529 Size: 35 3799 35 3977 Fragments arranged by size: 3977 3799 35 35 DraI TTT'AAA Cuts at: 5150 5150 Size: 7846 DraIII CAC_nnn'GTG Cuts at: 2608 2608 Size: 7846 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6853 809 Size: 507 5537 1802 Fragments arranged by size: 5537 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2129 3999 4238 Size: 54 1425 50 275 18 299 1870 239 Cuts at: 4238 4250 4331 5293 6182 7811 8 Size: 12 81 962 889 1629 43 Fragments arranged by size: 1870 1629 1425 962 889 299 275 239 81 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 5293 1537 Size: 275 3481 4090 Fragments arranged by size: 4090 3481 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4275 5776 1353 Size: 2922 1501 3423 Fragments arranged by size: 3423 2922 1501 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4832 6433 6807 6953 387 Size: 1064 138 3243 1601 374 146 1280 Fragments arranged by size: 3243 1601 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2702 2714 3060 3473 4496 4515 4579 Size: 393 1070 12 346 413 1023 19 64 Cuts at: 4579 4940 5015 5909 6506 7293 7561 1239 Size: 361 75 894 597 787 268 1524 Fragments arranged by size: 1524 1070 1023 894 787 597 413 393 361 346 268 75 64 19 12 EcoNI CCTnn'n_nnAGG Cuts at: 2717 2864 3887 2717 Size: 147 1023 6676 Fragments arranged by size: 6676 1023 147 EcoO109I rG'GnC_Cy Cuts at: 2141 2297 2572 2629 2630 2798 2857 2981 4186 Size: 156 275 57 1 168 59 124 1205 Cuts at: 4186 4318 5129 2141 Size: 132 811 4858 Fragments arranged by size: 4858 1205 811 275 168 156 132 124 59 57 1 EcoRI G'AATT_C Cuts at: 2927 5155 5533 2927 Size: 2228 378 5240 Fragments arranged by size: 5240 2228 378 EcoRV GAT'ATC Cuts at: 3122 3122 Size: 7846 FseI GG_CCGG'CC Cuts at: 2821 2821 Size: 7846 FspI TGC'GCA Cuts at: 4415 6352 4415 Size: 1937 5909 HaeII r_GCGC'y Cuts at: 1678 2012 2226 2256 2353 3759 4369 4994 6993 Size: 334 214 30 97 1406 610 625 1999 Cuts at: 6993 1678 Size: 2531 Fragments arranged by size: 2531 1999 1406 625 610 334 214 97 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2719 2751 3326 3358 6487 Size: 32 135 32 1147 32 575 32 3129 Cuts at: 6487 6519 6561 6593 1373 Size: 32 42 32 2626 Fragments arranged by size: 3129 2626 1147 575 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4775 5190 5436 669 Size: 988 281 2837 415 246 3079 Fragments arranged by size: 3079 2837 988 415 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7846 HpaI GTT'AAC Cuts at: 5436 5436 Size: 7846 KasI G'GCGC_C Cuts at: 2222 2349 3755 2222 Size: 127 1406 6313 Fragments arranged by size: 6313 1406 127 KpnI G_GTAC'C Cuts at: 5171 5171 Size: 7846 MfeI C'AATT_G Cuts at: 2485 3375 5445 2485 Size: 890 2070 4886 Fragments arranged by size: 4886 2070 890 MluI A'CGCG_T Cuts at: 7722 7722 Size: 7846 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3972 6960 7144 1305 Size: 2667 2988 184 2007 Fragments arranged by size: 2988 2667 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2131 4001 10 Size: 54 1425 343 299 1870 3855 Fragments arranged by size: 3855 1870 1425 343 299 54 MslI CAynn'nnrTG Cuts at: 518 1166 3531 4106 4640 5804 6163 6322 518 Size: 648 2365 575 534 1164 359 159 2042 Fragments arranged by size: 2365 2042 1164 648 575 534 359 159 MspA1I CmG'CkG Cuts at: 1606 2147 2248 4237 4325 4864 5262 5939 7087 Size: 541 101 1989 88 539 398 677 1148 Cuts at: 7087 7332 1606 Size: 245 2120 Fragments arranged by size: 2120 1989 1148 677 541 539 398 245 101 88 NaeI GCC'GGC Cuts at: 2819 2819 Size: 7846 NarI GG'CG_CC Cuts at: 2223 2350 3756 2223 Size: 127 1406 6313 Fragments arranged by size: 6313 1406 127 NcoI C'CATG_G Cuts at: 513 1065 2004 3192 3595 513 Size: 552 939 1188 403 4764 Fragments arranged by size: 4764 1188 939 552 403 NdeI CA'TA_TG Cuts at: 387 3714 387 Size: 3327 4519 NgoMIV G'CCGG_C Cuts at: 2817 2817 Size: 7846 NheI G'CTAG_C Cuts at: 1052 3467 1052 Size: 2415 5431 NotI GC'GGCC_GC Cuts at: 5293 5293 Size: 7846 NspI r_CATG'y Cuts at: 1132 1229 3475 3662 4111 5204 6749 1132 Size: 97 2246 187 449 1093 1545 2229 Fragments arranged by size: 2246 2229 1545 1093 449 187 97 PasI CC'CwG_GG Cuts at: 1348 3282 1348 Size: 1934 5912 PciI A'CATG_T Cuts at: 3658 6745 3658 Size: 3087 4759 PflMI CCAn_nnn'nTGG Cuts at: 1108 4340 4528 1108 Size: 3232 188 4426 Fragments arranged by size: 4426 3232 188 PfoI T'CCnGG_A Cuts at: 5134 5134 Size: 7846 PmeI GTTT'AAAC Cuts at: 5150 5150 Size: 7846 PmlI CAC'GTG Cuts at: 2807 2807 Size: 7846 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5915 5947 7428 7460 5915 Size: 32 1481 32 6301 Fragments arranged by size: 6301 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2141 2297 4318 5129 2141 Size: 156 2021 811 4858 Fragments arranged by size: 4858 2021 811 156 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7846 PsiI TTA'TAA Cuts at: 5416 5416 Size: 7846 PspOMI G'GGCC_C Cuts at: 2629 2980 2629 Size: 351 7495 PstI C_TGCA'G Cuts at: 830 2580 3229 3504 4149 5198 830 Size: 1750 649 275 645 1049 3478 Fragments arranged by size: 3478 1750 1049 649 645 275 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7846 PvuII CAG'CTG Cuts at: 4325 4864 4325 Size: 539 7307 SalI G'TCGA_C Cuts at: 1936 5188 1936 Size: 3252 4594 SanDI GG'GwC_CC Cuts at: 2141 2141 Size: 7846 SapI GCTCTTCn'nnn_ Cuts at: 4275 4275 Size: 7846 SbfI CC_TGCA'GG Cuts at: 2580 4149 5198 2580 Size: 1569 1049 5228 Fragments arranged by size: 5228 1569 1049 ScaI AGT'ACT Cuts at: 6094 7806 6094 Size: 1712 6134 SexAI A'CCwGG_T Cuts at: 2604 2604 Size: 7846 SfcI C'TryA_G Cuts at: 826 1045 2576 2811 3225 3500 4126 4145 5194 Size: 219 1531 235 414 275 626 19 1049 Cuts at: 5194 6329 7010 7201 826 Size: 1135 681 191 1471 Fragments arranged by size: 1531 1471 1135 1049 681 626 414 275 235 219 191 19 SfiI GGCCn_nnn'nGGCC Cuts at: 4761 4761 Size: 7846 SfoI GGC'GCC Cuts at: 2224 2351 3757 2224 Size: 127 1406 6313 Fragments arranged by size: 6313 1406 127 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7846 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7846 SmaI CCC'GGG Cuts at: 1872 2065 2843 2957 3077 5173 1872 Size: 193 778 114 120 2096 4545 Fragments arranged by size: 4545 2096 778 193 120 114 SmlI C'TyrA_G Cuts at: 820 1017 1943 2264 3938 5950 6851 7113 820 Size: 197 926 321 1674 2012 901 262 1553 Fragments arranged by size: 2012 1674 1553 926 901 321 262 197 SnaBI TAC'GTA Cuts at: 493 5637 493 Size: 5144 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7846 SphI G_CATG'C Cuts at: 1132 4111 5204 1132 Size: 2979 1093 3774 Fragments arranged by size: 3774 2979 1093 SspI AAT'ATT Cuts at: 5 52 5770 5 Size: 47 5718 2081 Fragments arranged by size: 5718 2081 47 StuI AGG'CCT Cuts at: 1517 2870 4469 4571 1517 Size: 1353 1599 102 4792 Fragments arranged by size: 4792 1599 1353 102 StyI C'CwwG_G Cuts at: 513 1065 2004 3192 3595 5019 5285 513 Size: 552 939 1188 403 1424 266 3074 Fragments arranged by size: 3074 1424 1188 939 552 403 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2441 2660 3180 3274 5873 5890 6043 Size: 1255 861 219 520 94 2599 17 153 Cuts at: 6043 6228 6673 325 Size: 185 445 1498 Fragments arranged by size: 2599 1498 1255 861 520 445 219 185 153 94 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4558 6092 7804 Size: 274 80 33 51 1443 2581 1534 1712 Cuts at: 7804 96 Size: 138 Fragments arranged by size: 2581 1712 1534 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2133 2165 2482 2514 4938 4970 7806 7838 2133 Size: 32 317 32 2424 32 2836 32 2141 Fragments arranged by size: 2836 2424 2141 317 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3179 1316 Size: 1863 5983 XbaI T'CTAG_A Cuts at: 3826 5182 3826 Size: 1356 6490 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3199 3955 3199 Size: 756 7090 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7846 XmaI C'CCGG_G Cuts at: 1870 2063 2841 2955 3075 5171 1870 Size: 193 778 114 120 2096 4545 Fragments arranged by size: 4545 2096 778 193 120 114 XmnI GAAnn'nnTTC Cuts at: 5975 5975 Size: 7846 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4123 5654 276 Size: 53 83 186 936 2589 1531 2468 Fragments arranged by size: 2589 2468 1531 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI Bsu36I EcoICRI FalI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SrfI SwaI