Restriction map for
pFN21AE3355
(Linear) (Six-base) MAPSORT of: KIEE3355.seq Check: 8061 from: 1 to: 3141
KIEE3355.orf
With 182 enzymes: SgfI *
August 27, 2018 19:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 31 3141
Size: 31 3110
AatII G_ACGT'C
Cuts at: 0 2120 3141
Size: 2120 1021
AccI GT'mk_AC
Cuts at: 0 2668 2705 3141
Size: 2668 37 436
Fragments arranged by size:
2668 436 37
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1055 2510 2574 2935 3010 3141
Size: 697 358 1455 64 361 75 131
Fragments arranged by size:
1455 697 361 358 131 75 64
AfeI AGC'GCT
Cuts at: 0 249 2987 3141
Size: 249 2738 154
Fragments arranged by size:
2738 249 154
AflIII A'CryG_T
Cuts at: 0 799 1653 3141
Size: 799 854 1488
Fragments arranged by size:
1488 854 799
AgeI A'CCGG_T
Cuts at: 0 1618 3141
Size: 1618 1523
AleI CACnn'nnGTG
Cuts at: 0 1526 3141
Size: 1526 1615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2653 3141
Size: 2653 488
AlwNI CAG_nnn'CTG
Cuts at: 0 1692 3141
Size: 1692 1449
ApaI G_GGCC'C
Cuts at: 0 628 979 3141
Size: 628 351 2162
Fragments arranged by size:
2162 628 351
ApoI r'AATT_y
Cuts at: 0 922 3141
Size: 922 2219
AseI AT'TA_AT
Cuts at: 0 1871 3141
Size: 1871 1270
AvaI C'yCGr_G
Cuts at: 0 58 611 628 836 950 1070 1233 3141
Size: 58 553 17 208 114 120 163 1908
Fragments arranged by size:
1908 553 208 163 120 114 58 17
BamHI G'GATC_C
Cuts at: 0 1019 1561 3141
Size: 1019 542 1580
Fragments arranged by size:
1580 1019 542
BanI G'GyrC_C
Cuts at: 0 217 237 344 1066 1750 2380 3141
Size: 217 20 107 722 684 630 761
Fragments arranged by size:
761 722 684 630 217 107 20
BanII G_rGCy'C
Cuts at: 0 628 951 979 1293 2310 3141
Size: 628 323 28 314 1017 831
Fragments arranged by size:
1017 831 628 323 314 28
BbeI G_GCGC'C
Cuts at: 0 221 348 1754 3141
Size: 221 127 1406 1387
Fragments arranged by size:
1406 1387 221 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 744 962 3141
Size: 744 218 2179
Fragments arranged by size:
2179 744 218
BbvCI CC'TCA_GC
Cuts at: 0 2191 2561 2860 3141
Size: 2191 370 299 281
Fragments arranged by size:
2191 370 299 281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 811 845 3141
Size: 811 34 2296
Fragments arranged by size:
2296 811 34
BclI T'GATC_A
Cuts at: 0 884 3141
Size: 884 2257
BglI GCCn_nnn'nGGC
Cuts at: 0 322 2756 3141
Size: 322 2434 385
Fragments arranged by size:
2434 385 322
BglII A'GATC_T
Cuts at: 0 1604 3141
Size: 1604 1537
BlpI GC'TnA_GC
Cuts at: 0 244 2321 3141
Size: 244 2077 820
Fragments arranged by size:
2077 820 244
Bme1580I G_kGCm'C
Cuts at: 0 177 476 628 979 1387 3141
Size: 177 299 152 351 408 1754
Fragments arranged by size:
1754 408 351 299 177 152
BmrI ACTGGGnnnn_n'
Cuts at: 0 1139 2894 3141
Size: 1139 1755 247
Fragments arranged by size:
1755 1139 247
BmtI G_CTAG'C
Cuts at: 0 1466 3141
Size: 1466 1675
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1468 2491 3141
Size: 709 759 1023 650
Fragments arranged by size:
1023 759 709 650
Bpu10I CC'TnA_GC
Cuts at: 0 106 2191 2309 2561 2860 3141
Size: 106 2085 118 252 299 281
Fragments arranged by size:
2085 299 281 252 118 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1918 3141
Size: 244 1674 1223
Fragments arranged by size:
1674 1223 244
BsaI GGTCTCn'nnnn_
Cuts at: 0 1187 3141
Size: 1187 1954
BsaAI yAC'GTr
Cuts at: 0 802 890 3141
Size: 802 88 2251
Fragments arranged by size:
2251 802 88
BsaHI Gr'CG_yC
Cuts at: 0 218 345 557 1751 2117 3141
Size: 218 127 212 1194 366 1024
Fragments arranged by size:
1194 1024 366 218 212 127
BsaWI w'CCGG_w
Cuts at: 0 1618 2258 3141
Size: 1618 640 883
Fragments arranged by size:
1618 883 640
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2537 2567 2933 2963 3141
Size: 2537 30 366 30 178
Fragments arranged by size:
2537 366 178 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1283 2994 3141
Size: 1283 1711 147
Fragments arranged by size:
1711 1283 147
BseYI C'CCAG_C
Cuts at: 0 444 972 1484 2316 2899 3141
Size: 444 528 512 832 583 242
Fragments arranged by size:
832 583 528 512 444 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 809 1550 3141
Size: 613 196 741 1591
Fragments arranged by size:
1591 741 613 196
BsiHKAI G_wGCw'C
Cuts at: 0 1973 2632 3141
Size: 1973 659 509
Fragments arranged by size:
1973 659 509
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2710 3141
Size: 2710 431
Bsp1286I G_dGCh'C
Cuts at: 0 177 476 628 951 979 1293 1387 1973
Size: 177 299 152 323 28 314 94 586
Cuts at: 1973 2310 2632 3141
Size: 337 322 509
Fragments arranged by size:
586 509 337 323 322 314 299 177
152 94 28
BspHI T'CATG_A
Cuts at: 0 1580 3141
Size: 1580 1561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 31 197 290 1677 2631 2680 3141
Size: 31 166 93 1387 954 49 461
Fragments arranged by size:
1387 954 461 166 93 49 31
BsrBI CCG'CTC
Cuts at: 0 2325 3117 3141
Size: 2325 792 24
Fragments arranged by size:
2325 792 24
BsrFI r'CCGG_y
Cuts at: 0 530 812 1618 3141
Size: 530 282 806 1523
Fragments arranged by size:
1523 806 530 282
BsrGI T'GTAC_A
Cuts at: 0 2553 3141
Size: 2553 588
BssSI C'ACGA_G
Cuts at: 0 1799 3141
Size: 1799 1342
BstBI TT'CG_AA
Cuts at: 0 1086 3141
Size: 1086 2055
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1950 2391 2433 3141
Size: 1950 441 42 708
Fragments arranged by size:
1950 708 441 42
BstYI r'GATC_y
Cuts at: 0 704 1019 1561 1604 2993 3141
Size: 704 315 542 43 1389 148
Fragments arranged by size:
1389 704 542 315 148 43
BstZ17I GTA'TAC
Cuts at: 0 2669 3141
Size: 2669 472
BtgI C'CryG_G
Cuts at: 0 127 182 1187 1590 3141
Size: 127 55 1005 403 1551
Fragments arranged by size:
1551 1005 403 127 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 981 1904 2313 3141
Size: 981 923 409 828
Fragments arranged by size:
981 923 828 409
BtsI GCAGTG_nn'
Cuts at: 0 175 2166 2954 3141
Size: 175 1991 788 187
Fragments arranged by size:
1991 788 187 175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2358 2393 3141
Size: 2358 35 748
Fragments arranged by size:
2358 748 35
DraIII CAC_nnn'GTG
Cuts at: 0 603 3141
Size: 603 2538
EaeI y'GGCC_r
Cuts at: 0 124 1994 2233 2245 2326 3141
Size: 124 1870 239 12 81 815
Fragments arranged by size:
1870 815 239 124 81 12
EarI CTCTTCn'nnn_
Cuts at: 0 2270 3141
Size: 2270 871
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2827 3141
Size: 2827 314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 709 1055 1468 2491 2510 2574 2935
Size: 697 12 346 413 1023 19 64 361
Cuts at: 2935 3010 3141
Size: 75 131
Fragments arranged by size:
1023 697 413 361 346 131 75 64
19 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 712 859 1882 3141
Size: 712 147 1023 1259
Fragments arranged by size:
1259 1023 712 147
EcoO109I rG'GnC_Cy
Cuts at: 0 136 292 567 624 625 793 852 976
Size: 136 156 275 57 1 168 59 124
Cuts at: 976 2181 2313 3124 3141
Size: 1205 132 811 17
Fragments arranged by size:
1205 811 275 168 156 136 132 124
59 57 17 1
EcoRI G'AATT_C
Cuts at: 0 922 3141
Size: 922 2219
EcoRV GAT'ATC
Cuts at: 0 1117 3141
Size: 1117 2024
FseI GG_CCGG'CC
Cuts at: 0 816 3141
Size: 816 2325
FspI TGC'GCA
Cuts at: 0 2410 3141
Size: 2410 731
HaeII r_GCGC'y
Cuts at: 0 7 221 251 348 1754 2364 2989 3141
Size: 7 214 30 97 1406 610 625 152
Fragments arranged by size:
1406 625 610 214 152 97 30 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 714 746 1321 1353 3141
Size: 714 32 575 32 1788
Fragments arranged by size:
1788 714 575 32 32
HincII GTy'rAC
Cuts at: 0 2770 3141
Size: 2770 371
KasI G'GCGC_C
Cuts at: 0 217 344 1750 3141
Size: 217 127 1406 1391
Fragments arranged by size:
1406 1391 217 127
MfeI C'AATT_G
Cuts at: 0 480 1370 3141
Size: 480 890 1771
Fragments arranged by size:
1771 890 480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1967 3141
Size: 1967 1174
MscI TGG'CCA
Cuts at: 0 126 1996 3141
Size: 126 1870 1145
Fragments arranged by size:
1870 1145 126
MslI CAynn'nnrTG
Cuts at: 0 1526 2101 2635 3141
Size: 1526 575 534 506
Fragments arranged by size:
1526 575 534 506
MspA1I CmG'CkG
Cuts at: 0 142 243 2232 2320 2859 3141
Size: 142 101 1989 88 539 282
Fragments arranged by size:
1989 539 282 142 101 88
NaeI GCC'GGC
Cuts at: 0 814 3141
Size: 814 2327
NarI GG'CG_CC
Cuts at: 0 218 345 1751 3141
Size: 218 127 1406 1390
Fragments arranged by size:
1406 1390 218 127
NcoI C'CATG_G
Cuts at: 0 1187 1590 3141
Size: 1187 403 1551
Fragments arranged by size:
1551 1187 403
NdeI CA'TA_TG
Cuts at: 0 1709 3141
Size: 1709 1432
NgoMIV G'CCGG_C
Cuts at: 0 812 3141
Size: 812 2329
NheI G'CTAG_C
Cuts at: 0 1462 3141
Size: 1462 1679
NspI r_CATG'y
Cuts at: 0 1470 1657 2106 3141
Size: 1470 187 449 1035
Fragments arranged by size:
1470 1035 449 187
PasI CC'CwG_GG
Cuts at: 0 1277 3141
Size: 1277 1864
PciI A'CATG_T
Cuts at: 0 1653 3141
Size: 1653 1488
PflMI CCAn_nnn'nTGG
Cuts at: 0 2335 2523 3141
Size: 2335 188 618
Fragments arranged by size:
2335 618 188
PfoI T'CCnGG_A
Cuts at: 0 3129 3141
Size: 3129 12
PmlI CAC'GTG
Cuts at: 0 802 3141
Size: 802 2339
PpuMI rG'GwC_Cy
Cuts at: 0 136 292 2313 3124 3141
Size: 136 156 2021 811 17
Fragments arranged by size:
2021 811 156 136 17
PspOMI G'GGCC_C
Cuts at: 0 624 975 3141
Size: 624 351 2166
Fragments arranged by size:
2166 624 351
PstI C_TGCA'G
Cuts at: 0 575 1224 1499 2144 3141
Size: 575 649 275 645 997
Fragments arranged by size:
997 649 645 575 275
PvuII CAG'CTG
Cuts at: 0 2320 2859 3141
Size: 2320 539 282
Fragments arranged by size:
2320 539 282
SanDI GG'GwC_CC
Cuts at: 0 136 3141
Size: 136 3005
SapI GCTCTTCn'nnn_
Cuts at: 0 2270 3141
Size: 2270 871
SbfI CC_TGCA'GG
Cuts at: 0 575 2144 3141
Size: 575 1569 997
Fragments arranged by size:
1569 997 575
SexAI A'CCwGG_T
Cuts at: 0 599 3141
Size: 599 2542
SfcI C'TryA_G
Cuts at: 0 571 806 1220 1495 2121 2140 3141
Size: 571 235 414 275 626 19 1001
Fragments arranged by size:
1001 626 571 414 275 235 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2756 3141
Size: 2756 385
SfoI GGC'GCC
Cuts at: 0 219 346 1752 3141
Size: 219 127 1406 1389
Fragments arranged by size:
1406 1389 219 127
SmaI CCC'GGG
Cuts at: 0 60 838 952 1072 3141
Size: 60 778 114 120 2069
Fragments arranged by size:
2069 778 120 114 60
SmlI C'TyrA_G
Cuts at: 0 259 1933 3141
Size: 259 1674 1208
Fragments arranged by size:
1674 1208 259
SphI G_CATG'C
Cuts at: 0 2106 3141
Size: 2106 1035
StuI AGG'CCT
Cuts at: 0 865 2464 2566 3141
Size: 865 1599 102 575
Fragments arranged by size:
1599 865 575 102
StyI C'CwwG_G
Cuts at: 0 1187 1590 3014 3141
Size: 1187 403 1424 127
Fragments arranged by size:
1424 1187 403 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 436 655 1175 1269 3141
Size: 436 219 520 94 1872
Fragments arranged by size:
1872 520 436 219 94
TatI w'GTAC_w
Cuts at: 0 2553 3141
Size: 2553 588
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 128 160 477 509 2933 2965 3141
Size: 128 32 317 32 2424 32 176
Fragments arranged by size:
2424 317 176 128 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1174 3141
Size: 1174 1967
XbaI T'CTAG_A
Cuts at: 0 1821 3141
Size: 1821 1320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1194 1950 3141
Size: 1194 756 1191
Fragments arranged by size:
1194 1191 756
XmaI C'CCGG_G
Cuts at: 0 58 836 950 1070 3141
Size: 58 778 114 120 2071
Fragments arranged by size:
2071 778 120 114 58
ZraI GAC'GTC
Cuts at: 0 2118 3141
Size: 2118 1023
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApoI AseI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI
DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FseI FspI HaeII Hin4I HincII KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SanDI SapI SbfI SexAI
SfcI SfiI SfoI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AhdI AloI ApaLI AscI AsiSI
AvrII BaeI BciVI BfrBI BmgBI BplI BsaBI BsiEI
BsiWI BsmI BspEI BsrDI BssHII BstAPI BstEII Bsu36I
ClaI DraI DrdI EagI EcoICRI FalI FspAI HindIII
HpaI KpnI MluI NotI NruI NsiI PacI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI ScaI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI XhoI XmnI