Restriction map for
pFN21AE3308
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AhdI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BbeI
BcgI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco52I EcoNI EcoO65I
EcoRV FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NarI NdeI NheI NotI NruI
NspV PacI PmeI PpuMI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI SwaI XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AgeI 2359 ACCGGT A^CCGGT
Aor51HI 851 AGCGCT AGC^GCT
AspI 409 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 863 CTCGAG C^[CT]CG[GA]G
BamHI 2005 GGATCC G^GATCC
BanI 340 GGTGCC G^G[CT][GA]CC
BbrPI 733 CACGTG CAC^GTG
BbvCI 27 CCTCAGC CCTCAGC(-5/-2)
BclI 624 TGATCA T^GATCA
BcnI 356 CCCGG CC^[GC]GG
BglII 2121 AGATCT A^GATCT
BsaI 1721 GAGACC GGTCTC(1/5)
BsaWI 2359 ACCGGT [AT]^CCGG[AT]
BseRI 1604 GAGGAG GAGGAG(10/8)
BsgI 239 CTGCAC GTGCAG(16/14)
BsmFI 1567 GGGAC GGGAC(10/14)
BsoBI 863 CTCGAG C^[CT]CG[GA]G
BstAPI 773 GCAACCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 852 GCGC GCG^C
DrdI 2244 GACGAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2205 TGGCCG [CT]^GGCC[GA]
Eco47III 851 AGCGCT AGC^GCT
EcoRI 103 GAATTC G^AATTC
FauI 2346 CCCGC CCCGC(4/6)
FbaI 624 TGATCA T^GATCA
HaeII 851 AGCGCT [GA]GCGC^[CT]
HhaI 852 GCGC GCG^C
HinP1I 852 GCGC G^CGC
HindIII 457 AAGCTT A^AGCTT
MspA1I 2134 CAGCTG C[AC]G^C[GT]G
NaeI 2207 GCCGGC GCC^GGC
NciI 356 CCCGG CC^[GC]GG
NgoMIV 2207 GCCGGC G^CCGGC
PaeR7I 863 CTCGAG C^TCGAG
PflFI 409 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2359 ACCGGT A^CCGGT
PmaCI 733 CACGTG CAC^GTG
PmlI 733 CACGTG CAC^GTG
PshAI 2079 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1279 CTGCAG CTGCA^G
PvuII 2134 CAGCTG CAG^CTG
SbfI 1278 CCTGCAGG CCTGCA^GG
SphI 1994 GCATGC GCATG^C
Sse8387I 1278 CCTGCAGG CCTGCA^GG
Tth111I 409 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 863 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 379 ACGTGT A^C[GA][CT]GT
734 ACGTGT
AlwI 2006 GATCC GGATC(4/5)
2005 GGATC
AocI 448 CCTGAGG CC^T[ACGT]AGG
1162 CCTGAGG
ApaI 20 GGGCCC GGGCC^C
2343 GGGCCC
BanII 20 GGGCCC G[GA]GC[CT]^C
2343 GGGCCC
BbsI 201 GAAGAC GAAGAC(2/6)
443 GAAGAC
BbvI 1209 GCAGC GCAGC(8/12)
2136 GCTGC
BpuAI 201 GAAGAC GAAGAC(2/6)
443 GAAGAC
BsaAI 378 TACGTG [CT]AC^GT[GA]
733 CACGTG
BsrDI 1779 CATTGC GCAATG(2/0)
1574 GCAATG
BsrFI 2207 GCCGGC [GA]^CCGG[CT]
2359 ACCGGT
BstYI 2005 GGATCC [GA]^GATC[CT]
2121 AGATCT
Bsu36I 448 CCTGAGG CC^T[ACGT]AGG
1162 CCTGAGG
Cfr10I 2207 GCCGGC [GA]^CCGG[CT]
2359 ACCGGT
DraII 812 AGGGCCT [GA]G^G[ACGT]CC[CT]
995 AGGGCCT
Eco81I 448 CCTGAGG CC^T[ACGT]AGG
1162 CCTGAGG
EcoO109I 812 AGGGCCT [GA]G^G[ACGT]CC[CT]
995 AGGGCCT
EcoT22I 544 ATGCAT ATGCA^T
1996 ATGCAT
MflI 2005 GGATCC [GA]^GATC[CT]
2121 AGATCT
NsiI 544 ATGCAT ATGCA^T
1996 ATGCAT
PflMI 2009 CCAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2133 CCAGCTGCTGG
PleI 1134 GACTC GAGTC(4/5)
2023 GAGTC
StuI 1275 AGGCCT AGG^CCT
2190 AGGCCT
TaqI 864 TCGA T^CGA
2046 TCGA
TseI 1209 GCAGC G^C[AT]GC
2136 GCTGC
TthHB8I 864 TCGA T^CGA
2046 TCGA
Van91I 2009 CCAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2133 CCAGCTGCTGG
XhoII 2005 GGATCC [GA]^GATC[CT]
2121 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI Asp700I AspHI AvaII BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I Tsp45I Tsp509I TspRI
XmnI