(Circular) (Six-base) MAPSORT of: pFN21AE3308.seq Check: 2028 from: 1 to: 7114 pFN21AE3308 7114 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3905 3905 Size: 7114 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4924 278 Size: 53 83 186 936 3388 2468 Fragments arranged by size: 3388 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4457 1937 Size: 2520 4594 Acc65I G'GTAC_C Cuts at: 4435 4435 Size: 7114 AclI AA'CG_TT Cuts at: 1563 5241 5614 1563 Size: 3678 373 3063 Fragments arranged by size: 3678 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 2166 2429 2771 3135 3451 5177 6561 Size: 410 124 263 342 364 316 1726 1384 Cuts at: 6561 6829 1632 Size: 268 1917 Fragments arranged by size: 1917 1726 1384 410 364 342 316 268 263 124 AfeI AGC'GCT Cuts at: 2858 2858 Size: 7114 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6917 AflIII A'CryG_T Cuts at: 1203 2384 2739 6013 6990 1203 Size: 1181 355 3274 977 1327 Fragments arranged by size: 3274 1327 1181 977 355 AgeI A'CCGG_T Cuts at: 4364 5945 4364 Size: 1581 5533 AhdI GACnn_n'nnGTC Cuts at: 5843 5843 Size: 7114 AleI CACnn'nnGTG Cuts at: 1166 3296 1166 Size: 2130 4984 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4277 1184 Size: 3093 4021 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3147 3179 3147 Size: 32 7082 AlwNI CAG_nnn'CTG Cuts at: 3004 3167 4144 4857 6429 3004 Size: 163 977 713 1572 3689 Fragments arranged by size: 3689 1572 977 713 163 ApaI G_GGCC'C Cuts at: 2029 4352 2029 Size: 2323 4791 ApaLI G'TGCA_C Cuts at: 5171 6327 5171 Size: 1156 5958 ApoI r'AATT_y Cuts at: 1493 2045 2108 3363 3374 3639 3827 3851 4423 Size: 552 63 1255 11 265 188 24 572 Cuts at: 4423 4649 4801 6910 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1255 572 552 265 226 188 152 63 24 11 AseI AT'TA_AT Cuts at: 160 1032 5668 160 Size: 872 4636 1606 Fragments arranged by size: 4636 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 AvaI C'yCGr_G Cuts at: 1870 1943 2868 4439 1870 Size: 73 925 1571 4545 Fragments arranged by size: 4545 1571 925 73 BamHI G'GATC_C Cuts at: 4010 4444 4811 4010 Size: 434 367 6313 Fragments arranged by size: 6313 434 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2345 4435 5890 618 Size: 325 217 627 558 2090 1455 1842 Fragments arranged by size: 2090 1842 1455 627 558 325 217 BanII G_rGCy'C Cuts at: 1391 2029 4352 4433 1391 Size: 638 2323 81 4072 Fragments arranged by size: 4072 2323 638 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2213 2455 928 Size: 971 314 242 5587 Fragments arranged by size: 5587 971 314 242 BbvCI CC'TCA_GC Cuts at: 2033 2033 Size: 7114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5305 5339 1903 Size: 34 3368 34 3678 Fragments arranged by size: 3678 3368 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5006 6216 5006 Size: 1210 5904 BclI T'GATC_A Cuts at: 1548 2629 1548 Size: 1081 6033 BfrBI ATG'CAT Cuts at: 2551 4003 2551 Size: 1452 5662 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5725 136 Size: 107 122 71 631 605 4053 1525 Fragments arranged by size: 4053 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4126 7109 4126 Size: 2983 4131 BlpI GC'TnA_GC Cuts at: 4531 4531 Size: 7114 Bme1580I G_kGCm'C Cuts at: 1790 2029 4352 5175 6331 1790 Size: 239 2323 823 1156 2573 Fragments arranged by size: 2573 2323 1156 823 239 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7114 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2947 3725 4845 Size: 416 53 83 399 24 1530 778 1120 Cuts at: 4845 5803 442 Size: 958 1753 Fragments arranged by size: 1753 1530 1120 958 778 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7114 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4275 4275 Size: 7114 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2672 3534 4166 5774 1239 Size: 1433 862 632 1608 2579 Fragments arranged by size: 2579 1608 1433 862 632 Bpu10I CC'TnA_GC Cuts at: 2033 2033 Size: 7114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2486 2588 5239 6104 6402 2486 Size: 102 2651 865 298 3198 Fragments arranged by size: 3198 2651 865 298 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3720 5777 882 Size: 617 2221 2057 2219 Fragments arranged by size: 2221 2219 2057 617 BsaAI yAC'GTr Cuts at: 493 1204 2385 2740 4905 493 Size: 711 1181 355 2165 2702 Fragments arranged by size: 2702 2165 1181 711 355 BsaBI GATnn'nnATC Cuts at: 7108 7108 Size: 7114 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4921 5303 5938 275 Size: 53 83 186 936 3388 382 635 1451 Fragments arranged by size: 3388 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4364 5547 5945 6219 6366 6777 4364 Size: 1183 398 274 147 411 4701 Fragments arranged by size: 4701 1183 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2356 2386 3147 3177 3717 Size: 30 165 30 953 30 761 30 540 Cuts at: 3717 3747 1178 Size: 30 4545 Fragments arranged by size: 4545 953 761 540 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3624 1186 Size: 2438 4676 BseYI C'CCAG_C Cuts at: 3210 3504 6317 6813 3210 Size: 294 2813 496 3511 Fragments arranged by size: 3511 2813 496 294 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2229 1163 Size: 573 493 6048 Fragments arranged by size: 6048 573 493 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4564 5325 6353 1540 Size: 275 186 2563 761 1028 2301 Fragments arranged by size: 2563 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2700 2875 4232 5175 5260 6331 2700 Size: 175 1357 943 85 1071 3483 Fragments arranged by size: 3483 1357 1071 943 175 85 BsmI GAATG_Cn' Cuts at: 4625 4718 4625 Size: 93 7021 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2700 2875 4232 4352 4433 5175 Size: 399 239 671 175 1357 120 81 742 Cuts at: 5175 5260 6331 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1357 1071 742 671 399 239 175 120 85 81 BspEI T'CCGG_A Cuts at: 6777 6777 Size: 7114 BspHI T'CATG_A Cuts at: 2202 2632 3804 5003 6733 2202 Size: 430 1172 1199 1730 2583 Fragments arranged by size: 2583 1730 1199 1172 430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2776 3277 3905 4469 844 Size: 1932 501 628 564 3489 Fragments arranged by size: 3489 1932 628 564 501 BsrBI CCG'CTC Cuts at: 1394 1823 5001 1394 Size: 429 3178 3507 Fragments arranged by size: 3507 3178 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3586 3783 5609 5783 65 Size: 1183 2338 197 1826 174 1396 Fragments arranged by size: 2338 1826 1396 1183 197 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4212 4364 5758 5945 1700 Size: 110 2402 152 1394 187 2869 Fragments arranged by size: 2869 2402 1394 187 152 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7114 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7114 BssSI C'ACGA_G Cuts at: 5174 6186 5174 Size: 1012 6102 BstAPI GCAn_nnn'nTGC Cuts at: 794 2784 794 Size: 1990 5124 BstBI TT'CG_AA Cuts at: 5918 5918 Size: 7114 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7114 BstXI CCAn_nnnn'nTGG Cuts at: 2120 3772 4177 2120 Size: 1652 405 5057 Fragments arranged by size: 5057 1652 405 BstYI r'GATC_y Cuts at: 1972 4010 4126 4444 4811 5196 5213 6665 6751 Size: 2038 116 318 367 385 17 1452 86 Cuts at: 6751 6763 7109 1972 Size: 12 346 1977 Fragments arranged by size: 2038 1977 1452 385 367 346 318 116 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2454 3168 2454 Size: 714 6400 BtgI C'CryG_G Cuts at: 513 1065 2113 3498 4170 4286 513 Size: 552 1048 1385 672 116 3341 Fragments arranged by size: 3341 1385 1048 672 552 116 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3616 487 Size: 683 56 2390 3985 Fragments arranged by size: 3985 2390 683 56 BtsI GCAGTG_nn' Cuts at: 2241 3038 4630 5424 5444 2241 Size: 797 1592 794 20 3911 Fragments arranged by size: 3911 1592 797 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3325 3360 529 Size: 35 2761 35 4283 Fragments arranged by size: 4283 2761 35 35 DraI TTT'AAA Cuts at: 4418 4418 Size: 7114 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4255 6121 809 Size: 507 2939 1866 1802 Fragments arranged by size: 2939 1866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4210 4561 5450 Size: 54 1425 50 275 18 2380 351 889 Cuts at: 5450 7079 8 Size: 1629 43 Fragments arranged by size: 2380 1629 1425 889 351 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4561 1537 Size: 275 2749 4090 Fragments arranged by size: 4090 2749 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5044 1353 Size: 3691 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5701 6075 6221 387 Size: 1064 138 4112 374 146 1280 Fragments arranged by size: 4112 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2166 2429 2672 2771 3135 3451 Size: 393 410 124 263 243 99 364 316 Cuts at: 3451 3534 4166 5177 5774 6561 6829 1239 Size: 83 632 1011 597 787 268 1524 Fragments arranged by size: 1524 1011 787 632 597 410 393 364 316 268 263 243 124 99 83 EcoO109I rG'GnC_Cy Cuts at: 2818 3001 2818 Size: 183 6931 EcoRI G'AATT_C Cuts at: 2108 4423 4801 2108 Size: 2315 378 4421 Fragments arranged by size: 4421 2315 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2180 2243 2180 Size: 63 7051 FspI TGC'GCA Cuts at: 5620 5620 Size: 7114 HaeII r_GCGC'y Cuts at: 1678 2860 6261 1678 Size: 1182 3401 2531 Fragments arranged by size: 3401 2531 1182 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2356 2388 3550 3582 3786 Size: 32 135 32 784 32 1162 32 204 Cuts at: 3786 3818 5755 5787 5829 5861 1373 Size: 32 1937 32 42 32 2626 Fragments arranged by size: 2626 1937 1162 784 204 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4458 4704 669 Size: 988 281 2520 246 3079 Fragments arranged by size: 3079 2520 988 281 246 HindIII A'AGCT_T Cuts at: 748 2462 748 Size: 1714 5400 HpaI GTT'AAC Cuts at: 4704 4704 Size: 7114 KpnI G_GTAC'C Cuts at: 4439 4439 Size: 7114 MfeI C'AATT_G Cuts at: 4713 4713 Size: 7114 MluI A'CGCG_T Cuts at: 6990 6990 Size: 7114 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3657 6228 6412 1305 Size: 2352 2571 184 2007 Fragments arranged by size: 2571 2352 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5292 Fragments arranged by size: 5292 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2118 3296 3770 4175 5072 5431 5590 Size: 648 952 1178 474 405 897 359 159 Cuts at: 5590 518 Size: 2042 Fragments arranged by size: 2042 1178 952 897 648 474 405 359 159 MspA1I CmG'CkG Cuts at: 1606 4141 4530 5207 6355 6600 1606 Size: 2535 389 677 1148 245 2120 Fragments arranged by size: 2535 2120 1148 677 389 245 NaeI GCC'GGC Cuts at: 4214 4214 Size: 7114 NcoI C'CATG_G Cuts at: 513 1065 2113 4170 4286 513 Size: 552 1048 2057 116 3341 Fragments arranged by size: 3341 2057 1048 552 116 NdeI CA'TA_TG Cuts at: 387 387 Size: 7114 NgoMIV G'CCGG_C Cuts at: 4212 4212 Size: 7114 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7114 NotI GC'GGCC_GC Cuts at: 4561 4561 Size: 7114 NsiI A_TGCA'T Cuts at: 2553 4005 2553 Size: 1452 5662 NspI r_CATG'y Cuts at: 1132 1229 2555 2797 4003 4472 6017 1132 Size: 97 1326 242 1206 469 1545 2229 Fragments arranged by size: 2229 1545 1326 1206 469 242 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7114 PciI A'CATG_T Cuts at: 6013 6013 Size: 7114 PflMI CCAn_nnn'nTGG Cuts at: 1108 4020 4144 1108 Size: 2912 124 4078 Fragments arranged by size: 4078 2912 124 PfoI T'CCnGG_A Cuts at: 2214 2508 2214 Size: 294 6820 PmeI GTTT'AAAC Cuts at: 4418 4418 Size: 7114 PmlI CAC'GTG Cuts at: 2740 2740 Size: 7114 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2632 2664 3147 3179 5183 5215 6696 6728 2632 Size: 32 483 32 2004 32 1481 32 3018 Fragments arranged by size: 3018 2004 1481 483 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 4088 1613 Size: 2475 4639 PsiI TTA'TAA Cuts at: 4684 4684 Size: 7114 PspOMI G'GGCC_C Cuts at: 2025 4348 2025 Size: 2323 4791 PspXI vC'TCGA_Gb Cuts at: 2868 2868 Size: 7114 PstI C_TGCA'G Cuts at: 830 3288 4466 830 Size: 2458 1178 3478 Fragments arranged by size: 3478 2458 1178 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7114 PvuII CAG'CTG Cuts at: 4141 4141 Size: 7114 SalI G'TCGA_C Cuts at: 1936 4456 1936 Size: 2520 4594 SbfI CC_TGCA'GG Cuts at: 3288 4466 3288 Size: 1178 5936 ScaI AGT'ACT Cuts at: 5362 7074 5362 Size: 1712 5402 SfcI C'TryA_G Cuts at: 826 1045 3267 3284 3918 4462 5597 6278 6469 Size: 219 2222 17 634 544 1135 681 191 Cuts at: 6469 826 Size: 1471 Fragments arranged by size: 2222 1471 1135 681 634 544 219 191 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7114 SmaI CCC'GGG Cuts at: 1872 4441 1872 Size: 2569 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2465 2567 2868 5218 6119 6381 Size: 197 926 522 102 301 2350 901 262 Cuts at: 6381 820 Size: 1553 Fragments arranged by size: 2350 1553 926 901 522 301 262 197 102 SnaBI TAC'GTA Cuts at: 493 4905 493 Size: 4412 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7114 SphI G_CATG'C Cuts at: 1132 4003 4472 1132 Size: 2871 469 3774 Fragments arranged by size: 3774 2871 469 SspI AAT'ATT Cuts at: 5 52 5038 5 Size: 47 4986 2081 Fragments arranged by size: 4986 2081 47 StuI AGG'CCT Cuts at: 1517 3282 4197 1517 Size: 1765 915 4434 Fragments arranged by size: 4434 1765 915 StyI C'CwwG_G Cuts at: 513 1065 2113 4170 4286 4553 513 Size: 552 1048 2057 116 267 3074 Fragments arranged by size: 3074 2057 1048 552 267 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3127 3327 3834 5141 5158 5311 5496 Size: 1255 1547 200 507 1307 17 153 185 Cuts at: 5496 5941 325 Size: 445 1498 Fragments arranged by size: 1547 1498 1307 1255 507 445 200 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2841 3940 5360 Size: 274 80 33 51 1443 864 1099 1420 Cuts at: 5360 7072 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1420 1099 864 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2724 2756 3717 3749 3780 3812 7074 7106 2724 Size: 32 961 32 31 32 3262 32 2732 Fragments arranged by size: 3262 2732 961 32 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 1316 2417 1316 Size: 1101 6013 XbaI T'CTAG_A Cuts at: 4450 4450 Size: 7114 XhoI C'TCGA_G Cuts at: 1943 2868 1943 Size: 925 6189 XmaI C'CCGG_G Cuts at: 1870 4439 1870 Size: 2569 4545 XmnI GAAnn'nnTTC Cuts at: 2210 2504 2661 5243 2210 Size: 294 157 2582 4081 Fragments arranged by size: 4081 2582 294 157 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4922 276 Size: 53 83 186 936 3388 2468 Fragments arranged by size: 3388 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PpuMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI