(Linear) (Six-base) MAPSORT of: KIEE3308.seq Check: 2930 from: 1 to: 2409 KIEE3308.orf With 182 enzymes: SgfI * July 3, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1900 2409 Size: 1900 509 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 161 424 766 1130 1446 2409 Size: 37 124 263 342 364 316 963 Fragments arranged by size: 963 364 342 316 263 124 37 AfeI AGC'GCT Cuts at: 0 853 2409 Size: 853 1556 AflIII A'CryG_T Cuts at: 0 379 734 2409 Size: 379 355 1675 Fragments arranged by size: 1675 379 355 AgeI A'CCGG_T Cuts at: 0 2359 2409 Size: 2359 50 AleI CACnn'nnGTG Cuts at: 0 1291 2409 Size: 1291 1118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2272 2409 Size: 2272 137 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1142 1174 2409 Size: 1142 32 1235 Fragments arranged by size: 1235 1142 32 AlwNI CAG_nnn'CTG Cuts at: 0 999 1162 2139 2409 Size: 999 163 977 270 Fragments arranged by size: 999 977 270 163 ApaI G_GGCC'C Cuts at: 0 24 2347 2409 Size: 24 2323 62 Fragments arranged by size: 2323 62 24 ApoI r'AATT_y Cuts at: 0 40 103 1358 1369 1634 1822 1846 2409 Size: 40 63 1255 11 265 188 24 563 Fragments arranged by size: 1255 563 265 188 63 40 24 11 AvaI C'yCGr_G Cuts at: 0 863 2409 Size: 863 1546 BamHI G'GATC_C Cuts at: 0 2005 2409 Size: 2005 404 BanI G'GyrC_C Cuts at: 0 340 2409 Size: 340 2069 BanII G_rGCy'C Cuts at: 0 24 2347 2409 Size: 24 2323 62 Fragments arranged by size: 2323 62 24 BbsI GAAGACnn'nnnn_ Cuts at: 0 208 450 2409 Size: 208 242 1959 Fragments arranged by size: 1959 242 208 BbvCI CC'TCA_GC Cuts at: 0 28 2409 Size: 28 2381 BclI T'GATC_A Cuts at: 0 624 2409 Size: 624 1785 BfrBI ATG'CAT Cuts at: 0 546 1998 2409 Size: 546 1452 411 Fragments arranged by size: 1452 546 411 BglII A'GATC_T Cuts at: 0 2121 2409 Size: 2121 288 Bme1580I G_kGCm'C Cuts at: 0 24 2347 2409 Size: 24 2323 62 Fragments arranged by size: 2323 62 24 BmrI ACTGGGnnnn_n' Cuts at: 0 942 1720 2409 Size: 942 778 689 Fragments arranged by size: 942 778 689 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2270 2409 Size: 2270 139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 667 1529 2161 2409 Size: 667 862 632 248 Fragments arranged by size: 862 667 632 248 Bpu10I CC'TnA_GC Cuts at: 0 28 2409 Size: 28 2381 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 481 583 2409 Size: 481 102 1826 Fragments arranged by size: 1826 481 102 BsaI GGTCTCn'nnnn_ Cuts at: 0 1715 2409 Size: 1715 694 BsaAI yAC'GTr Cuts at: 0 380 735 2409 Size: 380 355 1674 Fragments arranged by size: 1674 380 355 BsaWI w'CCGG_w Cuts at: 0 2359 2409 Size: 2359 50 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 351 381 1142 1172 1712 1742 2409 Size: 351 30 761 30 540 30 667 Fragments arranged by size: 761 667 540 351 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1619 2409 Size: 1619 790 BseYI C'CCAG_C Cuts at: 0 1205 1499 2409 Size: 1205 294 910 Fragments arranged by size: 1205 910 294 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 224 2409 Size: 224 2185 BsiHKAI G_wGCw'C Cuts at: 0 695 870 2227 2409 Size: 695 175 1357 182 Fragments arranged by size: 1357 695 182 175 Bsp1286I G_dGCh'C Cuts at: 0 24 695 870 2227 2347 2409 Size: 24 671 175 1357 120 62 Fragments arranged by size: 1357 671 175 120 62 24 BspHI T'CATG_A Cuts at: 0 197 627 1799 2409 Size: 197 430 1172 610 Fragments arranged by size: 1172 610 430 197 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 771 1272 1900 2409 Size: 771 501 628 509 Fragments arranged by size: 771 628 509 501 BsrDI GCAATG_nn' Cuts at: 0 1581 1778 2409 Size: 1581 197 631 Fragments arranged by size: 1581 631 197 BsrFI r'CCGG_y Cuts at: 0 2207 2359 2409 Size: 2207 152 50 Fragments arranged by size: 2207 152 50 BstAPI GCAn_nnn'nTGC Cuts at: 0 779 2409 Size: 779 1630 BstXI CCAn_nnnn'nTGG Cuts at: 0 115 1767 2172 2409 Size: 115 1652 405 237 Fragments arranged by size: 1652 405 237 115 BstYI r'GATC_y Cuts at: 0 2005 2121 2409 Size: 2005 116 288 Fragments arranged by size: 2005 288 116 Bsu36I CC'TnA_GG Cuts at: 0 449 1163 2409 Size: 449 714 1246 Fragments arranged by size: 1246 714 449 BtgI C'CryG_G Cuts at: 0 108 1493 2165 2281 2409 Size: 108 1385 672 116 128 Fragments arranged by size: 1385 672 128 116 108 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1611 2409 Size: 1611 798 BtsI GCAGTG_nn' Cuts at: 0 236 1033 2409 Size: 236 797 1376 Fragments arranged by size: 1376 797 236 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1320 1355 2409 Size: 1320 35 1054 Fragments arranged by size: 1320 1054 35 DrdI GACnn_nn'nnGTC Cuts at: 0 2250 2409 Size: 2250 159 EaeI y'GGCC_r Cuts at: 0 2205 2409 Size: 2205 204 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 161 424 667 766 1130 1446 1529 Size: 37 124 263 243 99 364 316 83 Cuts at: 1529 2161 2409 Size: 632 248 Fragments arranged by size: 632 364 316 263 248 243 124 99 83 37 EcoO109I rG'GnC_Cy Cuts at: 0 813 996 2409 Size: 813 183 1413 Fragments arranged by size: 1413 813 183 EcoRI G'AATT_C Cuts at: 0 103 2409 Size: 103 2306 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 175 238 2409 Size: 175 63 2171 Fragments arranged by size: 2171 175 63 HaeII r_GCGC'y Cuts at: 0 855 2409 Size: 855 1554 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 351 383 1545 1577 1781 1813 2409 Size: 351 32 1162 32 204 32 596 Fragments arranged by size: 1162 596 351 204 32 32 32 HindIII A'AGCT_T Cuts at: 0 457 2409 Size: 457 1952 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1652 2409 Size: 1652 757 MslI CAynn'nnrTG Cuts at: 0 113 1291 1765 2170 2409 Size: 113 1178 474 405 239 Fragments arranged by size: 1178 474 405 239 113 MspA1I CmG'CkG Cuts at: 0 2136 2409 Size: 2136 273 NaeI GCC'GGC Cuts at: 0 2209 2409 Size: 2209 200 NcoI C'CATG_G Cuts at: 0 108 2165 2281 2409 Size: 108 2057 116 128 Fragments arranged by size: 2057 128 116 108 NgoMIV G'CCGG_C Cuts at: 0 2207 2409 Size: 2207 202 NsiI A_TGCA'T Cuts at: 0 548 2000 2409 Size: 548 1452 409 Fragments arranged by size: 1452 548 409 NspI r_CATG'y Cuts at: 0 550 792 1998 2409 Size: 550 242 1206 411 Fragments arranged by size: 1206 550 411 242 PflMI CCAn_nnn'nTGG Cuts at: 0 2015 2139 2409 Size: 2015 124 270 Fragments arranged by size: 2015 270 124 PfoI T'CCnGG_A Cuts at: 0 209 503 2409 Size: 209 294 1906 Fragments arranged by size: 1906 294 209 PmlI CAC'GTG Cuts at: 0 735 2409 Size: 735 1674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 627 659 1142 1174 2409 Size: 627 32 483 32 1235 Fragments arranged by size: 1235 627 483 32 32 PshAI GACnn'nnGTC Cuts at: 0 2083 2409 Size: 2083 326 PspOMI G'GGCC_C Cuts at: 0 20 2343 2409 Size: 20 2323 66 Fragments arranged by size: 2323 66 20 PspXI vC'TCGA_Gb Cuts at: 0 863 2409 Size: 863 1546 PstI C_TGCA'G Cuts at: 0 1283 2409 Size: 1283 1126 PvuII CAG'CTG Cuts at: 0 2136 2409 Size: 2136 273 SbfI CC_TGCA'GG Cuts at: 0 1283 2409 Size: 1283 1126 SfcI C'TryA_G Cuts at: 0 1262 1279 1913 2409 Size: 1262 17 634 496 Fragments arranged by size: 1262 634 496 17 SmlI C'TyrA_G Cuts at: 0 460 562 863 2409 Size: 460 102 301 1546 Fragments arranged by size: 1546 460 301 102 SphI G_CATG'C Cuts at: 0 1998 2409 Size: 1998 411 StuI AGG'CCT Cuts at: 0 1277 2192 2409 Size: 1277 915 217 Fragments arranged by size: 1277 915 217 StyI C'CwwG_G Cuts at: 0 108 2165 2281 2409 Size: 108 2057 116 128 Fragments arranged by size: 2057 128 116 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1122 1322 1829 2409 Size: 1122 200 507 580 Fragments arranged by size: 1122 580 507 200 TatI w'GTAC_w Cuts at: 0 836 1935 2409 Size: 836 1099 474 Fragments arranged by size: 1099 836 474 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 719 751 1712 1744 1775 1807 2409 Size: 719 32 961 32 31 32 602 Fragments arranged by size: 961 719 602 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 0 412 2409 Size: 412 1997 XhoI C'TCGA_G Cuts at: 0 863 2409 Size: 863 1546 XmnI GAAnn'nnTTC Cuts at: 0 205 499 656 2409 Size: 205 294 157 1753 Fragments arranged by size: 1753 294 205 157 Enzymes that do cut: AarI AcuI AfeI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HindIII MmeI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PflMI PfoI PmlI PpiI PshAI PspOMI PspXI PstI PvuII SbfI SfcI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AhdI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BglI BlpI BmgBI BmtI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI DraI DraIII EagI EarI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI MscI NarI NdeI NheI NotI NruI PacI PasI PciI PmeI PpuMI PsiI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XbaI XcmI XmaI ZraI