(Circular) (Six-base) MAPSORT of: pFN21AB5882.seq Check: 9175 from: 1 to: 6193 pFN21AB5882 6193 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4003 278 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3536 1937 Size: 1599 4594 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2564 2747 2785 4256 5640 5908 1632 Size: 932 183 38 1471 1384 268 1917 Fragments arranged by size: 1917 1471 1384 932 268 183 38 AfeI AGC'GCT Cuts at: 2561 3332 2561 Size: 771 5422 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 5092 6069 1203 Size: 3889 977 1327 Fragments arranged by size: 3889 1327 977 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 2382 4922 2382 Size: 2540 3653 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6193 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6193 AlwNI CAG_nnn'CTG Cuts at: 2677 3936 5508 2677 Size: 1259 1572 3362 Fragments arranged by size: 3362 1572 1259 ApaI G_GGCC'C Cuts at: 2270 2820 3426 2270 Size: 550 606 5037 Fragments arranged by size: 5037 606 550 ApaLI G'TGCA_C Cuts at: 4250 5406 4250 Size: 1156 5037 ApoI r'AATT_y Cuts at: 1493 2948 3502 3728 3880 5989 1493 Size: 1455 554 226 152 2109 1697 Fragments arranged by size: 2109 1697 1455 554 226 152 AscI GG'CGCG_CC Cuts at: 2412 2412 Size: 6193 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2208 2272 2341 2354 2418 3334 Size: 73 80 185 64 69 13 64 916 Cuts at: 3334 3353 3518 1870 Size: 19 165 4545 Fragments arranged by size: 4545 916 185 165 80 73 69 64 64 19 13 BamHI G'GATC_C Cuts at: 3523 3890 3523 Size: 367 5826 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2231 2250 2626 3292 3514 Size: 325 217 627 444 19 376 666 222 Cuts at: 3514 4969 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 666 627 444 376 325 222 217 19 BanII G_rGCy'C Cuts at: 1391 2270 2312 2820 3195 3360 3426 3512 1391 Size: 879 42 508 375 165 66 86 4072 Fragments arranged by size: 4072 879 508 375 165 86 66 42 BbeI G_GCGC'C Cuts at: 2235 2254 2235 Size: 19 6174 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3145 3179 3388 3422 4384 4418 1903 Size: 34 1208 34 209 34 962 34 3678 Fragments arranged by size: 3678 1208 962 209 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4085 5295 4085 Size: 1210 4983 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2147 2447 2652 Size: 107 122 71 631 605 475 300 205 Cuts at: 2652 4804 136 Size: 2152 1525 Fragments arranged by size: 2152 1525 631 605 475 300 205 122 107 71 BglII A'GATC_T Cuts at: 6188 6188 Size: 6193 BlpI GC'TnA_GC Cuts at: 2133 3182 3610 2133 Size: 1049 428 4716 Fragments arranged by size: 4716 1049 428 Bme1580I G_kGCm'C Cuts at: 1790 2270 2820 3297 3426 4254 5410 1790 Size: 480 550 477 129 828 1156 2573 Fragments arranged by size: 2573 1156 828 550 480 477 129 BmgBI CAC'GTC Cuts at: 1318 2323 1318 Size: 1005 5188 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3022 3924 4882 Size: 416 53 83 399 24 1605 902 958 Cuts at: 4882 442 Size: 1753 Fragments arranged by size: 1753 1605 958 902 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2474 2528 2600 3143 3488 4853 1239 Size: 1235 54 72 543 345 1365 2579 Fragments arranged by size: 2579 1365 1235 543 345 72 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2537 4318 5183 5481 2537 Size: 1781 865 298 3249 Fragments arranged by size: 3249 1781 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4856 882 Size: 617 3357 2219 Fragments arranged by size: 3357 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2532 3984 493 Size: 711 1328 1452 2702 Fragments arranged by size: 2702 1452 1328 711 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2232 2251 2403 2424 Size: 53 83 186 936 699 19 152 21 Cuts at: 2424 2958 2976 3363 4000 4382 5017 275 Size: 534 18 387 637 382 635 1451 Fragments arranged by size: 1451 936 699 637 635 534 387 382 186 152 83 53 21 19 18 BsaWI w'CCGG_w Cuts at: 2971 3021 4626 5024 5298 5445 5856 2971 Size: 50 1605 398 274 147 411 3308 Fragments arranged by size: 3308 1605 411 398 274 147 50 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5968 Fragments arranged by size: 5968 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2844 3467 1186 Size: 1658 623 3912 Fragments arranged by size: 3912 1658 623 BseYI C'CCAG_C Cuts at: 2303 3417 5396 5892 2303 Size: 1114 1979 496 2604 Fragments arranged by size: 2604 1979 1114 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3019 1163 Size: 573 1283 4337 Fragments arranged by size: 4337 1283 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2068 2173 2686 3271 3643 4404 Size: 275 186 67 105 513 585 372 761 Cuts at: 4404 5432 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 585 513 372 275 186 105 67 BsiHKAI G_wGCw'C Cuts at: 2312 3021 3228 4254 4339 5410 2312 Size: 709 207 1026 85 1071 3095 Fragments arranged by size: 3095 1071 1026 709 207 85 BsmI GAATG_Cn' Cuts at: 3704 3797 3704 Size: 93 6100 BsmBI CGTCTCn'nnnn_ Cuts at: 2363 2363 Size: 6193 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2270 2312 2820 3021 3195 3228 3297 Size: 399 480 42 508 201 174 33 69 Cuts at: 3297 3360 3426 3512 4254 4339 5410 1391 Size: 63 66 86 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 508 480 399 201 174 86 85 69 66 63 42 33 BspEI T'CCGG_A Cuts at: 2971 5856 2971 Size: 2885 3308 BspHI T'CATG_A Cuts at: 4082 5812 4082 Size: 1730 4463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2387 2715 3548 844 Size: 1543 328 833 3489 Fragments arranged by size: 3489 1543 833 328 BsrBI CCG'CTC Cuts at: 1394 1823 2175 2398 4080 1394 Size: 429 352 223 1682 3507 Fragments arranged by size: 3507 1682 429 352 223 BsrDI GCAATG_nn' Cuts at: 65 1248 4688 4862 65 Size: 1183 3440 174 1396 Fragments arranged by size: 3440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2137 2253 2297 2408 2458 2805 4837 Size: 110 327 116 44 111 50 347 2032 Cuts at: 4837 5024 1700 Size: 187 2869 Fragments arranged by size: 2869 2032 347 327 187 116 111 110 50 44 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6193 BssHII G'CGCG_C Cuts at: 1925 2257 2412 2486 2632 1925 Size: 332 155 74 146 5486 Fragments arranged by size: 5486 332 155 146 74 BssSI C'ACGA_G Cuts at: 4253 5265 4253 Size: 1012 5181 BstAPI GCAn_nnn'nTGC Cuts at: 794 2677 794 Size: 1883 4310 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 2374 1184 Size: 1190 5003 BstYI r'GATC_y Cuts at: 1972 2878 3471 3523 3890 4275 4292 5744 5830 Size: 906 593 52 367 385 17 1452 86 Cuts at: 5830 5842 6188 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 906 593 385 367 346 86 52 17 12 Bsu36I CC'TnA_GG Cuts at: 3087 3087 Size: 6193 BtgI C'CryG_G Cuts at: 513 1065 2144 2198 2596 2718 3217 513 Size: 552 1079 54 398 122 499 3489 Fragments arranged by size: 3489 1079 552 499 398 122 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5454 Fragments arranged by size: 5454 683 56 BtsI GCAGTG_nn' Cuts at: 2223 2934 3709 4503 4523 2223 Size: 711 775 794 20 3893 Fragments arranged by size: 3893 794 775 711 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6158 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DraIII CAC_nnn'GTG Cuts at: 2719 2719 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3062 5200 809 Size: 507 1746 2138 1802 Fragments arranged by size: 2138 1802 1746 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2170 2439 2653 Size: 54 1425 50 275 18 340 269 214 Cuts at: 2653 2683 3268 3640 4529 6158 8 Size: 30 585 372 889 1629 43 Fragments arranged by size: 1629 1425 889 585 372 340 275 269 214 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2170 2683 3268 3640 1537 Size: 275 358 513 585 372 4090 Fragments arranged by size: 4090 585 513 372 358 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4123 1353 Size: 2770 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2174 2175 2342 2488 2633 2817 Size: 1064 138 585 1 167 146 145 184 Cuts at: 2817 4780 5154 5300 387 Size: 1963 374 146 1280 Fragments arranged by size: 1963 1280 1064 585 374 184 167 146 146 145 138 1 EcoICRI GAG'CTC Cuts at: 2310 2310 Size: 6193 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2474 2528 2564 2600 2747 2785 3143 Size: 393 842 54 36 36 147 38 358 Cuts at: 3143 3488 4256 4853 5640 5908 1239 Size: 345 768 597 787 268 1524 Fragments arranged by size: 1524 842 787 768 597 393 358 345 268 147 54 38 36 36 EcoNI CCTnn'n_nnAGG Cuts at: 3086 3086 Size: 6193 EcoO109I rG'GnC_Cy Cuts at: 2266 2267 3422 3423 2266 Size: 1 1155 1 5036 Fragments arranged by size: 5036 1155 1 1 EcoRI G'AATT_C Cuts at: 2948 3502 3880 2948 Size: 554 378 5261 Fragments arranged by size: 5261 554 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3184 3184 Size: 6193 FspI TGC'GCA Cuts at: 3287 4699 3287 Size: 1412 4781 HaeII r_GCGC'y Cuts at: 1678 2235 2254 2304 2563 3334 5340 1678 Size: 557 19 50 259 771 2006 2531 Fragments arranged by size: 2531 2006 771 557 259 50 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2368 2400 4834 4866 4908 Size: 32 135 32 796 32 2434 32 42 Cuts at: 4908 4940 1373 Size: 32 2626 Fragments arranged by size: 2626 2434 796 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3241 3537 3783 669 Size: 988 281 1303 296 246 3079 Fragments arranged by size: 3079 1303 988 296 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6193 HpaI GTT'AAC Cuts at: 3783 3783 Size: 6193 KasI G'GCGC_C Cuts at: 2231 2250 2231 Size: 19 6174 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 3792 3792 Size: 6193 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 5307 5491 1305 Size: 960 3042 184 2007 Fragments arranged by size: 3042 2007 960 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4371 Fragments arranged by size: 4371 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3314 4151 4510 4669 518 Size: 648 2148 837 359 159 2042 Fragments arranged by size: 2148 2042 837 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2146 2200 2598 2674 3096 3219 3609 Size: 479 61 54 398 76 422 123 390 Cuts at: 3609 4286 5434 5679 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 479 422 398 390 245 123 76 61 54 NaeI GCC'GGC Cuts at: 2139 2255 2299 2410 2460 2807 2139 Size: 116 44 111 50 347 5525 Fragments arranged by size: 5525 347 116 111 50 44 NarI GG'CG_CC Cuts at: 2232 2251 2232 Size: 19 6174 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5641 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NgoMIV G'CCGG_C Cuts at: 2137 2253 2297 2408 2458 2805 2137 Size: 116 44 111 50 347 5525 Fragments arranged by size: 5525 347 116 111 50 44 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 2170 3268 3640 2170 Size: 1098 372 4723 Fragments arranged by size: 4723 1098 372 NruI TCG'CGA Cuts at: 2481 2859 2481 Size: 378 5815 NspI r_CATG'y Cuts at: 1132 1229 2968 3551 5096 1132 Size: 97 1739 583 1545 2229 Fragments arranged by size: 2229 1739 1545 583 97 PasI CC'CwG_GG Cuts at: 1348 2236 1348 Size: 888 5305 PciI A'CATG_T Cuts at: 5092 5092 Size: 6193 PflMI CCAn_nnn'nTGG Cuts at: 1108 3417 1108 Size: 2309 3884 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4262 4294 5775 5807 4262 Size: 32 1481 32 4648 Fragments arranged by size: 4648 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PspOMI G'GGCC_C Cuts at: 2266 2816 3422 2266 Size: 550 606 5037 Fragments arranged by size: 5037 606 550 PstI C_TGCA'G Cuts at: 830 2712 3345 3545 830 Size: 1882 633 200 3478 Fragments arranged by size: 3478 1882 633 200 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 PvuII CAG'CTG Cuts at: 2674 2674 Size: 6193 SacI G_AGCT'C Cuts at: 2312 2312 Size: 6193 SacII CC_GC'GG Cuts at: 2147 2201 2599 3220 2147 Size: 54 398 621 5120 Fragments arranged by size: 5120 621 398 54 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 4441 6153 4441 Size: 1712 4481 SfcI C'TryA_G Cuts at: 826 1045 2708 3341 3541 4676 5357 5548 826 Size: 219 1663 633 200 1135 681 191 1471 Fragments arranged by size: 1663 1471 1135 681 633 219 200 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2447 2447 Size: 6193 SfoI GGC'GCC Cuts at: 2233 2252 2233 Size: 19 6174 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 2253 2408 1700 Size: 553 155 5485 Fragments arranged by size: 5485 553 155 SmaI CCC'GGG Cuts at: 1872 2210 2343 2420 3520 1872 Size: 338 133 77 1100 4545 Fragments arranged by size: 4545 1100 338 133 77 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 4297 5198 5460 820 Size: 197 926 609 1745 901 262 1553 Fragments arranged by size: 1745 1553 926 901 609 262 197 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SrfI GCCC'GGGC Cuts at: 2343 2343 Size: 6193 SspI AAT'ATT Cuts at: 5 52 4117 5 Size: 47 4065 2081 Fragments arranged by size: 4065 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6193 StyI C'CwwG_G Cuts at: 513 1065 3411 3632 513 Size: 552 2346 221 3074 Fragments arranged by size: 3074 2346 552 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4220 4237 4390 4575 5020 325 Size: 1255 2640 17 153 185 445 1498 Fragments arranged by size: 2640 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2865 4439 6151 Size: 274 80 33 51 1443 888 1574 1712 Cuts at: 6151 96 Size: 138 Fragments arranged by size: 1712 1574 1443 888 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6153 6185 6153 Size: 32 6161 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6193 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2434 2434 Size: 6193 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 2208 2341 2418 3518 1870 Size: 338 133 77 1100 4545 Fragments arranged by size: 4545 1100 338 133 77 XmnI GAAnn'nnTTC Cuts at: 4322 4322 Size: 6193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4001 276 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoRV FseI FspAI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SwaI