################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BspMI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 241 GGTACC G^GTACC AlwI 1041 GGATC GGATC(4/5) Asp718I 241 GGTACC G^GTACC BanII 2036 GAGCTC G[GA]GC[CT]^C BfaI 895 CTAG C^TAG BglI 2134 GCCTACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1923 AGATCT A^GATCT BsaBI 102 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 145 CTGCAC GTGCAG(16/14) BsmI 533 GCATTC GAATGC(1/-1) Bsp1407I 770 TGTACA T^GTACA BsrBI 80 GAGCGG CCGCTC(-3/-3) BsrDI 290 GCAATG GCAATG(2/0) BsrGI 770 TGTACA T^GTACA BstEII 1569 GGTAACC G^GT[ACGT]ACC BstPI 1569 GGTAACC G^GT[ACGT]ACC CfoI 62 GCGC GCG^C DsaI 222 CCATGG C^C[GA][CT]GG EarI 995 GAAGAG CTCTTC(1/4) EcoO65I 1569 GGTAACC G^GT[ACGT]ACC EcoT14I 222 CCATGG C^C[AT][AT]GG HhaI 62 GCGC GCG^C HinP1I 62 GCGC G^CGC HincII 2177 GTCAAC GT[CT]^[GA]AC HindII 2177 GTCAAC GT[CT]^[GA]AC KpnI 241 GGTACC GGTAC^C Ksp632I 995 GAAGAG CTCTTC(1/4) MaeI 895 CTAG C^TAG MaeII 149 ACGT A^CGT MamI 102 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 222 CCATGG C^CATGG NdeI 1676 CATATG CA^TATG NspI 1716 ACATGC [GA]CATG^[CT] PflMI 940 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 2045 CTGCAG CTGCA^G SacI 2036 GAGCTC GAGCT^C ScaI 1543 AGTACT AGT^ACT SspBI 770 TGTACA T^GTACA StuI 2272 AGGCCT AGG^CCT StyI 222 CCATGG C^C[AT][AT]GG SwaI 476 ATTTAAAT ATTT^AAAT TaqI 975 TCGA T^CGA Tsp45I 541 GTGAC ^GT[GC]AC TthHB8I 975 TCGA T^CGA Van91I 940 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 151 GTCTAC GT^[AC][GT]AC 1140 GTCTAC AccII 61 CGCG CG^CG 135 CGCG AflII 1912 CTTAAG C^TTAAG 2209 CTTAAG AspHI 647 GTGCTC G[AT]GC[AT]^C 2036 GAGCTC BanI 241 GGTACC G^G[CT][GA]CC 1074 GGCACC BclI 104 TGATCA T^GATCA 1094 TGATCA BcnI 65 CCCGG CC^[GC]GG 93 CCGGG BfrI 1912 CTTAAG C^TTAAG 2209 CTTAAG BmyI 647 GTGCTC G[AGT]GC[ACT]^C 2036 GAGCTC BseRI 16 CTCCTC GAGGAG(10/8) 194 GAGGAG BsiHKAI 647 GTGCTC G[AT]GC[AT]^C 2036 GAGCTC Bsp1286I 647 GTGCTC G[AGT]GC[ACT]^C 2036 GAGCTC BspHI 825 TCATGA T^CATGA 2189 TCATGA BsrFI 51 GCCGGT [GA]^CCGG[CT] 1071 ACCGGC BstAPI 1405 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1681 GCACTGCCTGC BstUI 61 CGCG CG^CG 135 CGCG BstYI 1041 GGATCT [GA]^GATC[CT] 1923 AGATCT Cfr10I 51 GCCGGT [GA]^CCGG[CT] 1071 ACCGGC EcoRV 199 GATATC GAT^ATC 2292 GATATC FauI 59 CCCGC CCCGC(4/6) 1777 GCGGG FbaI 104 TGATCA T^GATCA 1094 TGATCA MflI 1041 GGATCT [GA]^GATC[CT] 1923 AGATCT MslI 534 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 826 CATGATTGTG MvnI 61 CGCG CG^CG 135 CGCG NciI 65 CCCGG CC^[GC]GG 93 CCGGG PleI 1746 GACTC GAGTC(4/5) 1232 GAGTC RcaI 825 TCATGA T^CATGA 2189 TCATGA XhoII 1041 GGATCT [GA]^GATC[CT] 1923 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BalI BbvI BciVI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TfiI Tru9I TseI Tsp509I TspRI