(Circular) (Six-base) MAPSORT of: pFN21AB6296.seq Check: 3728 from: 1 to: 7081 pFN21AB6296 7081 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4891 278 Size: 53 83 186 936 3355 2468 Fragments arranged by size: 3355 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2157 3146 4424 1937 Size: 220 989 1278 4594 Fragments arranged by size: 4594 1278 989 220 Acc65I G'GTAC_C Cuts at: 2246 4402 2246 Size: 2156 4925 AclI AA'CG_TT Cuts at: 1563 5208 5581 1563 Size: 3645 373 3063 Fragments arranged by size: 3645 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3198 3462 3687 5144 6528 6796 1632 Size: 1566 264 225 1457 1384 268 1917 Fragments arranged by size: 1917 1566 1457 1384 268 264 225 AflII C'TTAA_G Cuts at: 820 1017 3917 4214 820 Size: 197 2900 297 3687 Fragments arranged by size: 3687 2900 297 197 AflIII A'CryG_T Cuts at: 1203 5980 6957 1203 Size: 4777 977 1327 Fragments arranged by size: 4777 1327 977 AgeI A'CCGG_T Cuts at: 5912 5912 Size: 7081 AhdI GACnn_n'nnGTC Cuts at: 5810 5810 Size: 7081 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7081 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7081 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3511 3543 3511 Size: 32 7049 AlwNI CAG_nnn'CTG Cuts at: 4824 6396 4824 Size: 1572 5509 ApaLI G'TGCA_C Cuts at: 5138 6294 5138 Size: 1156 5925 ApoI r'AATT_y Cuts at: 1493 2929 3810 4010 4059 4390 4616 4768 6877 Size: 1436 881 200 49 331 226 152 2109 Cuts at: 6877 1493 Size: 1697 Fragments arranged by size: 2109 1697 1436 881 331 226 200 152 49 AseI AT'TA_AT Cuts at: 160 1032 5635 160 Size: 872 4603 1606 Fragments arranged by size: 4603 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7081 AvaI C'yCGr_G Cuts at: 1870 1943 4406 1870 Size: 73 2463 4545 Fragments arranged by size: 4545 2463 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2886 2919 3525 3558 2886 Size: 33 606 33 6409 Fragments arranged by size: 6409 606 33 33 BamHI G'GATC_C Cuts at: 4411 4778 4411 Size: 367 6714 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2246 3079 4402 5857 618 Size: 325 217 627 459 833 1323 1455 1842 Fragments arranged by size: 1842 1455 1323 833 627 459 325 217 BanII G_rGCy'C Cuts at: 1391 4045 4400 1391 Size: 2654 355 4072 Fragments arranged by size: 4072 2654 355 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6110 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5272 5306 1903 Size: 34 3335 34 3678 Fragments arranged by size: 3678 3335 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2872 3553 3941 4091 4973 6183 2872 Size: 681 388 150 882 1210 3770 Fragments arranged by size: 3770 1210 882 681 388 150 BclI T'GATC_A Cuts at: 1548 2109 3099 1548 Size: 561 990 5530 Fragments arranged by size: 5530 990 561 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4145 5692 Size: 107 122 71 631 605 337 2136 1547 Cuts at: 5692 136 Size: 1525 Fragments arranged by size: 2136 1547 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3928 7076 3928 Size: 3148 3933 BlpI GC'TnA_GC Cuts at: 4498 4498 Size: 7081 Bme1580I G_kGCm'C Cuts at: 1790 5142 6298 1790 Size: 3352 1156 2573 Fragments arranged by size: 3352 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2155 1318 Size: 837 6244 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4812 5770 442 Size: 416 53 83 399 24 3395 958 1753 Fragments arranged by size: 3395 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7081 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2050 2196 4059 5741 1239 Size: 811 146 1863 1682 2579 Fragments arranged by size: 2579 1863 1682 811 146 Bpu10I CC'TnA_GC Cuts at: 3452 3452 Size: 7081 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3072 5206 6071 6369 3072 Size: 2134 865 298 3784 Fragments arranged by size: 3784 2134 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5744 882 Size: 617 4245 2219 Fragments arranged by size: 4245 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4872 493 Size: 711 3668 2702 Fragments arranged by size: 3668 2702 711 BsaBI GATnn'nnATC Cuts at: 2111 7075 2111 Size: 4964 2117 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4888 5270 5905 275 Size: 53 83 186 936 3355 382 635 1451 Fragments arranged by size: 3355 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5514 5912 6186 6333 6744 5514 Size: 398 274 147 411 5851 Fragments arranged by size: 5851 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6856 Fragments arranged by size: 6856 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 2214 1186 Size: 826 202 6053 Fragments arranged by size: 6053 826 202 BseYI C'CCAG_C Cuts at: 2015 3579 4238 6284 6780 2015 Size: 1564 659 2046 496 2316 Fragments arranged by size: 2316 2046 1564 659 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 1163 Size: 573 399 6109 Fragments arranged by size: 6109 573 399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4531 5292 6320 1540 Size: 275 186 2530 761 1028 2301 Fragments arranged by size: 2530 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2656 4045 5142 5227 6298 2656 Size: 1389 1097 85 1071 3439 Fragments arranged by size: 3439 1389 1097 1071 85 BsmI GAATG_Cn' Cuts at: 2538 4592 4685 2538 Size: 2054 93 4934 Fragments arranged by size: 4934 2054 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2656 4045 4400 5142 5227 6298 1391 Size: 399 866 1389 355 742 85 1071 2174 Fragments arranged by size: 2174 1389 1071 866 742 399 355 85 BspEI T'CCGG_A Cuts at: 6744 6744 Size: 7081 BspHI T'CATG_A Cuts at: 2830 4194 4970 6700 2830 Size: 1364 776 1730 3211 Fragments arranged by size: 3211 1730 1364 776 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4436 844 Size: 3592 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2087 4968 1394 Size: 429 264 2881 3507 Fragments arranged by size: 3507 2881 429 264 BsrDI GCAATG_nn' Cuts at: 65 1248 2302 5576 5750 65 Size: 1183 1054 3274 174 1396 Fragments arranged by size: 3274 1396 1183 1054 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2056 3076 5725 5912 1700 Size: 110 246 1020 2649 187 2869 Fragments arranged by size: 2869 2649 1020 246 187 110 BsrGI T'GTAC_A Cuts at: 96 2775 96 Size: 2679 4402 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7081 BssSI C'ACGA_G Cuts at: 5141 6153 5141 Size: 1012 6069 BstAPI GCAn_nnn'nTGC Cuts at: 794 3416 3692 794 Size: 2622 276 4183 Fragments arranged by size: 4183 2622 276 BstBI TT'CG_AA Cuts at: 5885 5885 Size: 7081 BstEII G'GTnAC_C Cuts at: 1184 3574 1184 Size: 2390 4691 BstYI r'GATC_y Cuts at: 1972 3046 3928 4411 4778 5163 5180 6632 6718 Size: 1074 882 483 367 385 17 1452 86 Cuts at: 6718 6730 7076 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1074 882 483 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2227 513 Size: 552 939 223 5367 Fragments arranged by size: 5367 939 552 223 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2122 487 Size: 683 56 896 5446 Fragments arranged by size: 5446 896 683 56 BtsI GCAGTG_nn' Cuts at: 3686 4597 5391 5411 3686 Size: 911 794 20 5356 Fragments arranged by size: 5356 911 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7081 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3747 3782 3961 3996 4325 4360 529 Size: 35 3183 35 179 35 329 35 3250 Fragments arranged by size: 3250 3183 329 179 35 35 35 35 DraI TTT'AAA Cuts at: 2484 3367 3817 4015 4377 4385 2484 Size: 883 450 198 362 8 5180 Fragments arranged by size: 5180 883 450 362 198 8 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6088 809 Size: 507 4772 1802 Fragments arranged by size: 4772 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2953 3768 Size: 54 1425 50 275 18 261 862 815 Cuts at: 3768 4528 5417 7046 8 Size: 760 889 1629 43 Fragments arranged by size: 1629 1425 889 862 815 760 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4528 1537 Size: 275 2716 4090 Fragments arranged by size: 4090 2716 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2995 5011 1353 Size: 1642 2016 3423 Fragments arranged by size: 3423 2016 1642 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5668 6042 6188 387 Size: 1064 138 4079 374 146 1280 Fragments arranged by size: 4079 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4043 4043 Size: 7081 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2050 2196 3198 3462 3687 4059 5144 Size: 393 418 146 1002 264 225 372 1085 Cuts at: 5144 5741 6528 6796 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1085 1002 787 597 418 393 372 268 264 225 146 EcoO109I rG'GnC_Cy Cuts at: 2794 3467 3989 2794 Size: 673 522 5886 Fragments arranged by size: 5886 673 522 EcoRI G'AATT_C Cuts at: 4390 4768 4390 Size: 378 6703 EcoRV GAT'ATC Cuts at: 2206 4299 2206 Size: 2093 4988 FspI TGC'GCA Cuts at: 5587 5587 Size: 7081 HaeII r_GCGC'y Cuts at: 1678 6228 1678 Size: 4550 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2190 2222 2812 2844 3128 Size: 32 135 32 618 32 590 32 284 Cuts at: 3128 3160 3235 3267 4256 4288 5722 5754 5796 Size: 32 75 32 989 32 1434 32 42 Cuts at: 5796 5828 1373 Size: 32 2626 Fragments arranged by size: 2626 1434 989 618 590 284 135 75 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4184 4425 4671 669 Size: 988 281 2246 241 246 3079 Fragments arranged by size: 3079 2246 988 281 246 241 HindIII A'AGCT_T Cuts at: 748 748 Size: 7081 HpaI GTT'AAC Cuts at: 4671 4671 Size: 7081 KpnI G_GTAC'C Cuts at: 2250 4406 2250 Size: 2156 4925 MfeI C'AATT_G Cuts at: 4680 4680 Size: 7081 MluI A'CGCG_T Cuts at: 6957 6957 Size: 7081 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2481 2513 4127 6195 6379 1305 Size: 1176 32 1614 2068 184 2007 Fragments arranged by size: 2068 2007 1614 1176 184 32 MscI TGG'CCA Cuts at: 10 64 1489 1832 2093 2955 3770 10 Size: 54 1425 343 261 862 815 3321 Fragments arranged by size: 3321 1425 862 815 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2543 2835 5039 5398 5557 518 Size: 648 1377 292 2204 359 159 2042 Fragments arranged by size: 2204 2042 1377 648 359 292 159 MspA1I CmG'CkG Cuts at: 1606 2192 2588 3097 4497 5174 6322 6567 1606 Size: 586 396 509 1400 677 1148 245 2120 Fragments arranged by size: 2120 1400 1148 677 586 509 396 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2227 513 Size: 552 939 223 5367 Fragments arranged by size: 5367 939 552 223 NdeI CA'TA_TG Cuts at: 387 3682 387 Size: 3295 3786 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7081 NotI GC'GGCC_GC Cuts at: 4528 4528 Size: 7081 NspI r_CATG'y Cuts at: 1132 1229 3725 4439 5984 1132 Size: 97 2496 714 1545 2229 Fragments arranged by size: 2496 2229 1545 714 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7081 PciI A'CATG_T Cuts at: 5980 5980 Size: 7081 PflMI CCAn_nnn'nTGG Cuts at: 1108 2951 1108 Size: 1843 5238 PfoI T'CCnGG_A Cuts at: 2097 4036 2097 Size: 1939 5142 PmeI GTTT'AAAC Cuts at: 4385 4385 Size: 7081 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5150 5182 6663 6695 5150 Size: 32 1481 32 5536 Fragments arranged by size: 5536 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2794 3467 3989 2794 Size: 673 522 5886 Fragments arranged by size: 5886 673 522 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7081 PsiI TTA'TAA Cuts at: 4651 4651 Size: 7081 PstI C_TGCA'G Cuts at: 830 4054 4433 830 Size: 3224 379 3478 Fragments arranged by size: 3478 3224 379 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7081 PvuII CAG'CTG Cuts at: 2192 2588 3097 2192 Size: 396 509 6176 Fragments arranged by size: 6176 509 396 SacI G_AGCT'C Cuts at: 4045 4045 Size: 7081 SalI G'TCGA_C Cuts at: 1936 4423 1936 Size: 2487 4594 SanDI GG'GwC_CC Cuts at: 2794 2794 Size: 7081 SbfI CC_TGCA'GG Cuts at: 4433 4433 Size: 7081 ScaI AGT'ACT Cuts at: 3550 5329 7041 3550 Size: 1779 1712 3590 Fragments arranged by size: 3590 1779 1712 SfcI C'TryA_G Cuts at: 826 1045 2507 3147 3726 3907 4050 4429 5564 Size: 219 1462 640 579 181 143 379 1135 Cuts at: 5564 6245 6436 826 Size: 681 191 1471 Fragments arranged by size: 1471 1462 1135 681 640 579 379 219 191 181 143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7081 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7081 SmaI CCC'GGG Cuts at: 1872 4408 1872 Size: 2536 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3087 3917 4214 5185 6086 6348 Size: 197 926 1144 830 297 971 901 262 Cuts at: 6348 820 Size: 1553 Fragments arranged by size: 1553 1144 971 926 901 830 297 262 197 SnaBI TAC'GTA Cuts at: 493 4872 493 Size: 4379 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7081 SphI G_CATG'C Cuts at: 1132 4439 1132 Size: 3307 3774 SspI AAT'ATT Cuts at: 5 52 2256 4123 4133 5005 5 Size: 47 2204 1867 10 872 2081 Fragments arranged by size: 2204 2081 1867 872 47 10 StuI AGG'CCT Cuts at: 1517 4279 1517 Size: 2762 4319 StyI C'CwwG_G Cuts at: 513 1065 2004 2227 4520 513 Size: 552 939 223 2293 3074 Fragments arranged by size: 3074 2293 939 552 223 SwaI ATTT'AAAT Cuts at: 2484 2484 Size: 7081 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5108 5125 5278 5463 5908 325 Size: 1255 3528 17 153 185 445 1498 Fragments arranged by size: 3528 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2775 3091 3176 Size: 274 80 33 51 1443 798 316 85 Cuts at: 3176 3548 3608 5327 7039 96 Size: 372 60 1719 1712 138 Fragments arranged by size: 1719 1712 1443 798 372 316 274 138 85 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4192 4224 7041 7073 4192 Size: 32 2817 32 4200 Fragments arranged by size: 4200 2817 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7081 XbaI T'CTAG_A Cuts at: 4417 4417 Size: 7081 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7081 XmaI C'CCGG_G Cuts at: 1870 4406 1870 Size: 2536 4545 XmnI GAAnn'nnTTC Cuts at: 5210 5210 Size: 7081 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4889 276 Size: 53 83 186 936 3355 2468 Fragments arranged by size: 3355 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI XcmI