(Linear) (Six-base) MAPSORT of: KIBB6296.seq Check: 8061 from: 1 to: 2376 KIBB6296.orf With 182 enzymes: SgfI * July 3, 2012 16:20 .. AccI GT'mk_AC Cuts at: 0 152 1141 2376 Size: 152 989 1235 Fragments arranged by size: 1235 989 152 Acc65I G'GTAC_C Cuts at: 0 241 2376 Size: 241 2135 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1193 1457 1682 2376 Size: 1193 264 225 694 Fragments arranged by size: 1193 694 264 225 AflII C'TTAA_G Cuts at: 0 1912 2209 2376 Size: 1912 297 167 Fragments arranged by size: 1912 297 167 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1506 1538 2376 Size: 1506 32 838 Fragments arranged by size: 1506 838 32 ApoI r'AATT_y Cuts at: 0 924 1805 2005 2054 2376 Size: 924 881 200 49 322 Fragments arranged by size: 924 881 322 200 49 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 881 914 1520 1553 2376 Size: 881 33 606 33 823 Fragments arranged by size: 881 823 606 33 33 BanI G'GyrC_C Cuts at: 0 241 1074 2376 Size: 241 833 1302 Fragments arranged by size: 1302 833 241 BanII G_rGCy'C Cuts at: 0 2040 2376 Size: 2040 336 BciVI GTATCCnnnnn_n' Cuts at: 0 867 1548 1936 2086 2376 Size: 867 681 388 150 290 Fragments arranged by size: 867 681 388 290 150 BclI T'GATC_A Cuts at: 0 104 1094 2376 Size: 104 990 1282 Fragments arranged by size: 1282 990 104 BglI GCCn_nnn'nGGC Cuts at: 0 2140 2376 Size: 2140 236 BglII A'GATC_T Cuts at: 0 1923 2376 Size: 1923 453 BmgBI CAC'GTC Cuts at: 0 150 2376 Size: 150 2226 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 45 191 2054 2376 Size: 45 146 1863 322 Fragments arranged by size: 1863 322 146 45 Bpu10I CC'TnA_GC Cuts at: 0 1447 2376 Size: 1447 929 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1067 2376 Size: 1067 1309 BsaBI GATnn'nnATC Cuts at: 0 106 2376 Size: 106 2270 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 7 209 2376 Size: 7 202 2167 Fragments arranged by size: 2167 202 7 BseYI C'CCAG_C Cuts at: 0 10 1574 2233 2376 Size: 10 1564 659 143 Fragments arranged by size: 1564 659 143 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 2376 Size: 130 2246 BsiHKAI G_wGCw'C Cuts at: 0 651 2040 2376 Size: 651 1389 336 Fragments arranged by size: 1389 651 336 BsmI GAATG_Cn' Cuts at: 0 533 2376 Size: 533 1843 Bsp1286I G_dGCh'C Cuts at: 0 651 2040 2376 Size: 651 1389 336 Fragments arranged by size: 1389 651 336 BspHI T'CATG_A Cuts at: 0 825 2189 2376 Size: 825 1364 187 Fragments arranged by size: 1364 825 187 BsrBI CCG'CTC Cuts at: 0 82 2376 Size: 82 2294 BsrDI GCAATG_nn' Cuts at: 0 297 2376 Size: 297 2079 BsrFI r'CCGG_y Cuts at: 0 51 1071 2376 Size: 51 1020 1305 Fragments arranged by size: 1305 1020 51 BsrGI T'GTAC_A Cuts at: 0 770 2376 Size: 770 1606 BstAPI GCAn_nnn'nTGC Cuts at: 0 1411 1687 2376 Size: 1411 276 689 Fragments arranged by size: 1411 689 276 BstEII G'GTnAC_C Cuts at: 0 1569 2376 Size: 1569 807 BstYI r'GATC_y Cuts at: 0 1041 1923 2376 Size: 1041 882 453 Fragments arranged by size: 1041 882 453 BtgI C'CryG_G Cuts at: 0 222 2376 Size: 222 2154 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 117 2376 Size: 117 2259 BtsI GCAGTG_nn' Cuts at: 0 1681 2376 Size: 1681 695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1742 1777 1956 1991 2320 2355 2376 Size: 1742 35 179 35 329 35 21 Fragments arranged by size: 1742 329 179 35 35 35 21 DraI TTT'AAA Cuts at: 0 479 1362 1812 2010 2372 2376 Size: 479 883 450 198 362 4 Fragments arranged by size: 883 479 450 362 198 4 EaeI y'GGCC_r Cuts at: 0 86 948 1763 2376 Size: 86 862 815 613 Fragments arranged by size: 862 815 613 86 EarI CTCTTCn'nnn_ Cuts at: 0 990 2376 Size: 990 1386 EcoICRI GAG'CTC Cuts at: 0 2038 2376 Size: 2038 338 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 45 191 1193 1457 1682 2054 2376 Size: 45 146 1002 264 225 372 322 Fragments arranged by size: 1002 372 322 264 225 146 45 EcoO109I rG'GnC_Cy Cuts at: 0 789 1462 1984 2376 Size: 789 673 522 392 Fragments arranged by size: 789 673 522 392 EcoRV GAT'ATC Cuts at: 0 201 2294 2376 Size: 201 2093 82 Fragments arranged by size: 2093 201 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 185 217 807 839 1123 1155 1230 1262 Size: 185 32 590 32 284 32 75 32 Cuts at: 1262 2251 2283 2376 Size: 989 32 93 Fragments arranged by size: 989 590 284 185 93 75 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2179 2376 Size: 2179 197 KpnI G_GTAC'C Cuts at: 0 245 2376 Size: 245 2131 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 476 508 2122 2376 Size: 476 32 1614 254 Fragments arranged by size: 1614 476 254 32 MscI TGG'CCA Cuts at: 0 88 950 1765 2376 Size: 88 862 815 611 Fragments arranged by size: 862 815 611 88 MslI CAynn'nnrTG Cuts at: 0 538 830 2376 Size: 538 292 1546 Fragments arranged by size: 1546 538 292 MspA1I CmG'CkG Cuts at: 0 187 583 1092 2376 Size: 187 396 509 1284 Fragments arranged by size: 1284 509 396 187 NcoI C'CATG_G Cuts at: 0 222 2376 Size: 222 2154 NdeI CA'TA_TG Cuts at: 0 1677 2376 Size: 1677 699 NspI r_CATG'y Cuts at: 0 1720 2376 Size: 1720 656 PflMI CCAn_nnn'nTGG Cuts at: 0 946 2376 Size: 946 1430 PfoI T'CCnGG_A Cuts at: 0 92 2031 2376 Size: 92 1939 345 Fragments arranged by size: 1939 345 92 PpuMI rG'GwC_Cy Cuts at: 0 789 1462 1984 2376 Size: 789 673 522 392 Fragments arranged by size: 789 673 522 392 PstI C_TGCA'G Cuts at: 0 2049 2376 Size: 2049 327 PvuII CAG'CTG Cuts at: 0 187 583 1092 2376 Size: 187 396 509 1284 Fragments arranged by size: 1284 509 396 187 SacI G_AGCT'C Cuts at: 0 2040 2376 Size: 2040 336 SanDI GG'GwC_CC Cuts at: 0 789 2376 Size: 789 1587 ScaI AGT'ACT Cuts at: 0 1545 2376 Size: 1545 831 SfcI C'TryA_G Cuts at: 0 502 1142 1721 1902 2045 2376 Size: 502 640 579 181 143 331 Fragments arranged by size: 640 579 502 331 181 143 SmlI C'TyrA_G Cuts at: 0 1082 1912 2209 2376 Size: 1082 830 297 167 Fragments arranged by size: 1082 830 297 167 SspI AAT'ATT Cuts at: 0 251 2118 2128 2376 Size: 251 1867 10 248 Fragments arranged by size: 1867 251 248 10 StuI AGG'CCT Cuts at: 0 2274 2376 Size: 2274 102 StyI C'CwwG_G Cuts at: 0 222 2376 Size: 222 2154 SwaI ATTT'AAAT Cuts at: 0 479 2376 Size: 479 1897 TatI w'GTAC_w Cuts at: 0 770 1086 1171 1543 1603 2376 Size: 770 316 85 372 60 773 Fragments arranged by size: 773 770 372 316 85 60 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2187 2219 2376 Size: 2187 32 157 Fragments arranged by size: 2187 157 32 Enzymes that do cut: AccI Acc65I AcuI AflII AloI ApoI BaeI BanI BanII BciVI BclI BglI BglII BmgBI BpmI Bpu10I BpuEI BsaBI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstYI BtgI BtgZI BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI NdeI NspI PflMI PfoI PpuMI PstI PvuII SacI SanDI ScaI SfcI SmlI SspI StuI StyI SwaI TatI TstI Enzymes that do not cut: AarI AatII AclI AfeI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI BbsI BbvCI BcgI BfrBI BlpI Bme1580I BmrI BmtI BplI BsaI BsaAI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspMI BssHII BssSI BstBI BstXI BstZ17I Bsu36I ClaI DraIII DrdI EagI EciI EcoNI EcoRI FalI FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI