Restriction map for
pFN21AE3357
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsgI BsiEI BsiHKAI
BsiWI BsoBI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
FauI FseI FspI HaeII Hin1I
KasI KspI MamI MluI MluNI
MroI MscI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 700 GGTACC G^GTACC
AccI 2425 GTCTAC GT^[AC][GT]AC
AccII 33 CGCG CG^CG
AflII 745 CTTAAG C^TTAAG
AlwNI 1523 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 700 GGTACC G^GTACC
AvaII 1324 GGTCC G^G[AT]CC
BanII 1484 GAGCCC G[GA]GC[CT]^C
BciVI 1577 GGATAC GTATCC(6/5)
BclI 1839 TGATCA T^GATCA
BcnI 1574 CCCGG CC^[GC]GG
BfrI 745 CTTAAG C^TTAAG
BglII 993 AGATCT A^GATCT
BsaI 2207 GAGACC GGTCTC(1/5)
Bsp1407I 1958 TGTACA T^GTACA
BspHI 2870 TCATGA T^CATGA
BsrGI 1958 TGTACA T^GTACA
BstBI 2827 TTCGAA TT^CGAA
BstUI 33 CGCG CG^CG
BstYI 993 AGATCT [GA]^GATC[CT]
DraIII 1430 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 329 TGGCCG [CT]^GGCC[GA]
EcoRI 1056 GAATTC G^AATTC
EcoRV 2769 GATATC GAT^ATC
FbaI 1839 TGATCA T^GATCA
HpaI 1107 GTTAAC GTT^AAC
HphI 1513 TCACC GGTGA(8/7)
KpnI 700 GGTACC GGTAC^C
MflI 993 AGATCT [GA]^GATC[CT]
MvnI 33 CGCG CG^CG
NciI 1574 CCCGG CC^[GC]GG
NcoI 881 CCATGG C^CATGG
NspV 2827 TTCGAA TT^CGAA
PflMI 1429 CCACCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 22 CTGCAG CTGCA^G
RcaI 2870 TCATGA T^CATGA
SapI 1900 GAAGAGC GCTCTTC(1/4)
ScaI 921 AGTACT AGT^ACT
SfuI 2827 TTCGAA TT^CGAA
SspBI 1958 TGTACA T^GTACA
Van91I 1429 CCACCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2220 TCTAGA T^CTAGA
XcmI 1803 CCAAGCTAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 993 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 2816 CCGC CCGC(-3/-1)
34 GCGG
AlwI 721 GATCC GGATC(4/5)
2841 GGATC
AseI 88 ATTAAT AT^TAAT
1734 ATTAAT
AsnI 88 ATTAAT AT^TAAT
1734 ATTAAT
BbvCI 184 CCTCAGC CCTCAGC(-5/-2)
672 GCTGAGG
BmyI 1484 GAGCCC G[AGT]GC[ACT]^C
1816 GGGCAC
BsaWI 1321 TCCGGT [AT]^CCGG[AT]
2046 ACCGGA
BsmBI 2153 CGTCTC CGTCTC(1/5)
853 GAGACG
BsmI 171 GAATGC GAATGC(1/-1)
346 GCATTC
Bsp1286I 1484 GAGCCC G[AGT]GC[ACT]^C
1816 GGGCAC
BspDI 1772 ATCGAT AT^CGAT
2960 ATCGAT
BsrDI 227 GCAATG GCAATG(2/0)
1018 GCAATG
BstAPI 12 GCAGAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2113 GCACAACTTGC
CfoI 32 GCGC GCG^C
37 GCGC
Cfr13I 1324 GGTCC G^G[ACGT]CC
2373 GGCCC
ClaI 1772 ATCGAT AT^CGAT
2960 ATCGAT
DsaI 5 CCGTGG C^C[GA][CT]GG
881 CCATGG
EcoT22I 1361 ATGCAT ATGCA^T
1597 ATGCAT
HgaI 1417 GACGC GACGC(5/10)
2152 GCGTC
HhaI 32 GCGC GCG^C
37 GCGC
HinP1I 32 GCGC G^CGC
37 GCGC
HincII 1107 GTTAAC GT[CT]^[GA]AC
1405 GTTGAC
HindII 1107 GTTAAC GT[CT]^[GA]AC
1405 GTTGAC
HindIII 2129 AAGCTT A^AGCTT
2143 AAGCTT
MaeII 17 ACGT A^CGT
55 ACGT
MfeI 314 CAATTG C^AATTG
1727 CAATTG
MunI 314 CAATTG C^AATTG
1727 CAATTG
NsiI 1361 ATGCAT ATGCA^T
1597 ATGCAT
PshBI 88 ATTAAT AT^TAAT
1734 ATTAAT
Sau96I 1324 GGTCC G^G[ACGT]CC
2373 GGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BanI BbsI BbvI BfaI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cac8I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI