(Circular) (Six-base) MAPSORT of: pFN21AE3357.seq Check: 6567 from: 1 to: 7870 pFN21AE3357 7870 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5680 278 Size: 53 83 186 936 4144 2468 Fragments arranged by size: 4144 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4431 5213 1937 Size: 2494 782 4594 Fragments arranged by size: 4594 2494 782 Acc65I G'GTAC_C Cuts at: 2705 5191 2705 Size: 2486 5384 AclI AA'CG_TT Cuts at: 1563 5997 6370 1563 Size: 4434 373 3063 Fragments arranged by size: 4434 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 2871 2963 3344 3483 4593 5933 7317 Size: 662 577 92 381 139 1110 1340 1384 Cuts at: 7317 7585 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1340 1110 662 577 381 268 139 92 AflII C'TTAA_G Cuts at: 820 1017 2750 820 Size: 197 1733 5940 Fragments arranged by size: 5940 1733 197 AflIII A'CryG_T Cuts at: 1203 6769 7746 1203 Size: 5566 977 1327 Fragments arranged by size: 5566 1327 977 AgeI A'CCGG_T Cuts at: 6701 6701 Size: 7870 AhdI GACnn_n'nnGTC Cuts at: 6599 6599 Size: 7870 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7870 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7870 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4989 5021 4989 Size: 32 7838 AlwNI CAG_nnn'CTG Cuts at: 3533 5613 7185 3533 Size: 2080 1572 4218 Fragments arranged by size: 4218 2080 1572 ApaLI G'TGCA_C Cuts at: 5927 7083 5927 Size: 1156 6714 ApoI r'AATT_y Cuts at: 1493 2579 2656 2745 2942 3061 3137 3168 3185 Size: 1086 77 89 197 119 76 31 17 Cuts at: 3185 3374 4501 4622 4663 5179 5405 5557 7666 Size: 189 1127 121 41 516 226 152 2109 Cuts at: 7666 1493 Size: 1697 Fragments arranged by size: 2109 1697 1127 1086 516 226 197 189 152 121 119 89 77 76 41 31 17 AseI AT'TA_AT Cuts at: 160 1032 2094 3740 6424 160 Size: 872 1062 1646 2684 1606 Fragments arranged by size: 2684 1646 1606 1062 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7870 AvaI C'yCGr_G Cuts at: 1870 1943 5195 1870 Size: 73 3252 4545 Fragments arranged by size: 4545 3252 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2233 2259 2266 2292 2233 Size: 26 7 26 7811 Fragments arranged by size: 7811 26 26 7 BamHI G'GATC_C Cuts at: 5200 5567 5200 Size: 367 7503 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2705 3610 4450 5191 6646 Size: 325 217 627 918 905 840 741 1455 Cuts at: 6646 618 Size: 1842 Fragments arranged by size: 1842 1455 918 905 840 741 627 325 217 BanII G_rGCy'C Cuts at: 1391 3493 5189 1391 Size: 2102 1696 4072 Fragments arranged by size: 4072 2102 1696 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3163 4083 4392 928 Size: 971 1264 920 309 4406 Fragments arranged by size: 4406 1264 971 920 309 BbvCI CC'TCA_GC Cuts at: 2190 2678 2190 Size: 488 7382 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6061 6095 1903 Size: 34 4124 34 3678 Fragments arranged by size: 4124 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3576 5762 6972 3576 Size: 2186 1210 4474 Fragments arranged by size: 4474 2186 1210 BclI T'GATC_A Cuts at: 1548 3844 1548 Size: 2296 5574 BfrBI ATG'CAT Cuts at: 3368 3604 3368 Size: 236 7634 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6481 136 Size: 107 122 71 631 605 4809 1525 Fragments arranged by size: 4809 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2998 7865 2998 Size: 4867 3003 BlpI GC'TnA_GC Cuts at: 5287 5287 Size: 7870 Bme1580I G_kGCm'C Cuts at: 1790 3825 5931 7087 1790 Size: 2035 2106 1156 2573 Fragments arranged by size: 2573 2106 2035 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7870 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3838 4026 5601 Size: 416 53 83 399 24 2421 188 1575 Cuts at: 5601 6559 442 Size: 958 1753 Fragments arranged by size: 2421 1753 1575 958 416 399 188 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7870 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2484 2484 Size: 7870 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2310 3573 3688 4860 6530 1239 Size: 1071 1263 115 1172 1670 2579 Fragments arranged by size: 2579 1670 1263 1172 1071 115 Bpu10I CC'TnA_GC Cuts at: 2190 2678 2190 Size: 488 7382 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2347 2607 3367 4530 5995 6860 7158 2347 Size: 260 760 1163 1465 865 298 3059 Fragments arranged by size: 3059 1465 1163 865 760 298 260 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4206 6533 882 Size: 617 2707 2327 2219 Fragments arranged by size: 2707 2327 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5661 493 Size: 711 4457 2702 Fragments arranged by size: 4457 2702 711 BsaBI GATnn'nnATC Cuts at: 7864 7864 Size: 7870 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5677 6059 6694 275 Size: 53 83 186 936 4144 382 635 1451 Fragments arranged by size: 4144 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3326 4051 6303 6701 6975 7122 7533 3326 Size: 725 2252 398 274 147 411 3663 Fragments arranged by size: 3663 2252 725 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4989 5019 1178 Size: 30 165 30 3586 30 4029 Fragments arranged by size: 4029 3586 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2213 2870 4013 4613 1186 Size: 1027 657 1143 600 4443 Fragments arranged by size: 4443 1143 1027 657 600 BseYI C'CCAG_C Cuts at: 3818 7073 7569 3818 Size: 3255 496 4119 Fragments arranged by size: 4119 3255 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5320 6081 7109 1540 Size: 275 186 3319 761 1028 2301 Fragments arranged by size: 3319 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5931 6016 7087 5931 Size: 85 1071 6714 Fragments arranged by size: 6714 1071 85 BsmI GAATG_Cn' Cuts at: 2182 2351 5381 5474 2182 Size: 169 3030 93 4578 Fragments arranged by size: 4578 3030 169 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2852 4164 2852 Size: 1312 6558 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3493 3825 5189 5931 6016 7087 1391 Size: 399 1703 332 1364 742 85 1071 2174 Fragments arranged by size: 2174 1703 1364 1071 742 399 332 85 BspEI T'CCGG_A Cuts at: 7533 7533 Size: 7870 BspHI T'CATG_A Cuts at: 4875 5759 7489 4875 Size: 884 1730 5256 Fragments arranged by size: 5256 1730 884 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5225 844 Size: 4381 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5757 1394 Size: 429 3934 3507 Fragments arranged by size: 3934 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2239 3030 6365 6539 65 Size: 1183 991 791 3335 174 1396 Fragments arranged by size: 3335 1396 1183 991 791 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6514 6701 1700 Size: 110 4704 187 2869 Fragments arranged by size: 4704 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3963 96 Size: 3867 4003 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7870 BssSI C'ACGA_G Cuts at: 5930 6942 5930 Size: 1012 6858 BstAPI GCAn_nnn'nTGC Cuts at: 794 2023 4124 794 Size: 1229 2101 4540 Fragments arranged by size: 4540 2101 1229 BstBI TT'CG_AA Cuts at: 4833 6674 4833 Size: 1841 6029 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7870 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 7870 BstYI r'GATC_y Cuts at: 1972 2998 5200 5567 5952 5969 7421 7507 7519 Size: 1026 2202 367 385 17 1452 86 12 Cuts at: 7519 7865 1972 Size: 346 1977 Fragments arranged by size: 2202 1977 1452 1026 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2010 2886 513 Size: 552 945 876 5497 Fragments arranged by size: 5497 945 876 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3647 487 Size: 683 56 2421 4710 Fragments arranged by size: 4710 2421 683 56 BtsI GCAGTG_nn' Cuts at: 2160 5386 6180 6200 2160 Size: 3226 794 20 3830 Fragments arranged by size: 3830 3226 794 20 ClaI AT'CG_AT Cuts at: 1554 3778 4966 1554 Size: 2224 1188 4458 Fragments arranged by size: 4458 2224 1188 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4038 4073 529 Size: 35 3474 35 4326 Fragments arranged by size: 4326 3474 35 35 DraI TTT'AAA Cuts at: 5174 5174 Size: 7870 DraIII CAC_nnn'GTG Cuts at: 3440 3440 Size: 7870 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6877 809 Size: 507 5561 1802 Fragments arranged by size: 5561 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2334 5317 6206 Size: 54 1425 50 275 18 504 2983 889 Cuts at: 6206 7835 8 Size: 1629 43 Fragments arranged by size: 2983 1629 1425 889 504 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5317 1537 Size: 275 3505 4090 Fragments arranged by size: 4090 3505 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2432 2847 2862 3249 3900 4783 4835 5161 Size: 1079 415 15 387 651 883 52 326 Cuts at: 5161 5800 1353 Size: 639 3423 Fragments arranged by size: 3423 1079 883 651 639 415 387 326 52 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6457 6831 6977 387 Size: 1064 138 4868 374 146 1280 Fragments arranged by size: 4868 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2310 2871 2963 3344 3483 3573 Size: 393 662 16 561 92 381 139 90 Cuts at: 3573 3688 4593 4860 5933 6530 7317 7585 1239 Size: 115 905 267 1073 597 787 268 1524 Fragments arranged by size: 1524 1073 905 787 662 597 561 393 381 268 267 139 115 92 90 16 EcoRI G'AATT_C Cuts at: 3061 5179 5557 3061 Size: 2118 378 5374 Fragments arranged by size: 5374 2118 378 EcoRV GAT'ATC Cuts at: 4776 4776 Size: 7870 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2324 2324 Size: 7870 FspI TGC'GCA Cuts at: 6376 6376 Size: 7870 HaeII r_GCGC'y Cuts at: 1678 7017 1678 Size: 5339 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4220 4252 6511 6543 6585 Size: 32 135 32 2648 32 2259 32 42 Cuts at: 6585 6617 1373 Size: 32 2626 Fragments arranged by size: 2648 2626 2259 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3114 3412 5214 5460 669 Size: 988 281 1176 298 1802 246 3079 Fragments arranged by size: 3079 1802 1176 988 298 281 246 HindIII A'AGCT_T Cuts at: 748 4134 4148 748 Size: 3386 14 4470 Fragments arranged by size: 4470 3386 14 HpaI GTT'AAC Cuts at: 3114 5460 3114 Size: 2346 5524 KpnI G_GTAC'C Cuts at: 2709 5195 2709 Size: 2486 5384 MfeI C'AATT_G Cuts at: 2319 3732 5469 2319 Size: 1413 1737 4720 Fragments arranged by size: 4720 1737 1413 MluI A'CGCG_T Cuts at: 7746 7746 Size: 7870 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4332 6984 7168 1305 Size: 3027 2652 184 2007 Fragments arranged by size: 3027 2652 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6048 Fragments arranged by size: 6048 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2249 2957 4305 5828 6187 6346 Size: 648 843 240 708 1348 1523 359 159 Cuts at: 6346 518 Size: 2042 Fragments arranged by size: 2042 1523 1348 843 708 648 359 240 159 MspA1I CmG'CkG Cuts at: 1606 5286 5963 7111 7356 1606 Size: 3680 677 1148 245 2120 Fragments arranged by size: 3680 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2886 513 Size: 552 1821 5497 Fragments arranged by size: 5497 1821 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7870 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7870 NotI GC'GGCC_GC Cuts at: 5317 5317 Size: 7870 NsiI A_TGCA'T Cuts at: 3370 3606 3370 Size: 236 7634 NspI r_CATG'y Cuts at: 1132 1229 2433 3220 3788 5228 6773 1132 Size: 97 1204 787 568 1440 1545 2229 Fragments arranged by size: 2229 1545 1440 1204 787 568 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7870 PciI A'CATG_T Cuts at: 6769 6769 Size: 7870 PflMI CCAn_nnn'nTGG Cuts at: 1108 3440 1108 Size: 2332 5538 PfoI T'CCnGG_A Cuts at: 3578 3578 Size: 7870 PmeI GTTT'AAAC Cuts at: 5174 5174 Size: 7870 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4989 5021 5939 5971 7452 7484 4989 Size: 32 918 32 1481 32 5375 Fragments arranged by size: 5375 1481 918 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7870 PsiI TTA'TAA Cuts at: 5440 5440 Size: 7870 PstI C_TGCA'G Cuts at: 830 2031 5222 830 Size: 1201 3191 3478 Fragments arranged by size: 3478 3191 1201 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7870 SalI G'TCGA_C Cuts at: 1936 5212 1936 Size: 3276 4594 SapI GCTCTTCn'nnn_ Cuts at: 3900 3900 Size: 7870 SbfI CC_TGCA'GG Cuts at: 5222 5222 Size: 7870 ScaI AGT'ACT Cuts at: 2928 6118 7830 2928 Size: 3190 1712 2968 Fragments arranged by size: 3190 2968 1712 SfcI C'TryA_G Cuts at: 826 1045 2027 2111 2448 4947 5218 6353 7034 Size: 219 982 84 337 2499 271 1135 681 Cuts at: 7034 7225 826 Size: 191 1471 Fragments arranged by size: 2499 1471 1135 982 681 337 271 219 191 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7870 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7870 SmaI CCC'GGG Cuts at: 1872 5197 1872 Size: 3325 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2326 2622 2750 3382 4545 5974 Size: 197 926 383 296 128 632 1163 1429 Cuts at: 5974 6875 7137 820 Size: 901 262 1553 Fragments arranged by size: 1553 1429 1163 926 901 632 383 296 262 197 128 SnaBI TAC'GTA Cuts at: 493 5661 493 Size: 5168 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7870 SphI G_CATG'C Cuts at: 1132 5228 1132 Size: 4096 3774 SspI AAT'ATT Cuts at: 5 52 5794 5 Size: 47 5742 2081 Fragments arranged by size: 5742 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7870 StyI C'CwwG_G Cuts at: 513 1065 2886 3345 3614 4290 4981 5309 513 Size: 552 1821 459 269 676 691 328 3074 Fragments arranged by size: 3074 1821 691 676 552 459 328 269 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2117 5897 5914 6067 6252 6697 325 Size: 1255 537 3780 17 153 185 445 1498 Fragments arranged by size: 3780 1498 1255 537 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2926 3744 3870 Size: 274 80 33 51 1443 949 818 126 Cuts at: 3870 3963 6116 7828 96 Size: 93 2153 1712 138 Fragments arranged by size: 2153 1712 1443 949 818 274 138 126 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7830 7862 7830 Size: 32 7838 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7870 XbaI T'CTAG_A Cuts at: 4225 5206 4225 Size: 981 6889 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3815 3815 Size: 7870 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7870 XmaI C'CCGG_G Cuts at: 1870 5195 1870 Size: 3325 4545 XmnI GAAnn'nnTTC Cuts at: 3168 4275 4665 5999 3168 Size: 1107 390 1334 5039 Fragments arranged by size: 5039 1334 1107 390 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5678 276 Size: 53 83 186 936 4144 2468 Fragments arranged by size: 4144 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI